Effects of an Autoregulatory Senescence-inhibitor Gene Construct on Nicotiana alata Link and Otto.

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 519d-519 ◽  
Author(s):  
Kenneth R. Schroeder ◽  
Dennis P. Stimart
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Height ◽  
Dry Weight ◽  
Nicotiana Alata ◽  
Wild Type ◽  
Gene Encoding ◽  
Delayed Senescence ◽  
Gene Construct ◽  
Shoot Dry Weight ◽  
Inhibitor System

Nicotiana alata Link and Otto. was transformed via Agrobacterium tumefaciens encoding a senescence-specific promoter SAG12 cloned from Arabidopsis thaliana fused to a Agrobacterium tumefaciens gene encoding isopentenyl transferase (IPT) that catalyzes cytokinin synthesis. This was considered an autoregulatory senescence-inhibitor system. In 1996, we reported delayed senescence of intact flowers by 2 to 6 d and delayed leaf senescence of transgenic vs. wild-type N. alata. Further evaluations in 1997 revealed several other interesting effects of the SAG12-IPT gene construct. Measurement of chlorophyll content of mature leaves showed higher levels of both chlorophyll a and b in transgenic material under normal fertilization and truncated fertilization regimes. At 4 to 5 months of age transgenic plants expressed differences in plant height, branching, and dry weight. Plant height was reduced by 3 to 13 cm; branch counts increased 2 to 3 fold; and shoot dry weight increased up to 11 g over wild-type N. alata. These observations indicate the system is not tightly autoregulated and may prove useful to the floriculture industry for producing compact and more floriferous plants.

scholarly journals Effect of Salinity Levels (NaCl) on Yield, Yield Components and Quality Content of Sesame (Sesamum Indicum L.) Cultivars

2016 ◽  
Vol 5 (2) ◽  
pp. 104
Author(s):  
Helale Bahrami ◽  
Amir Ostadi Jafari ◽  
Jamshid Razmjoo
Keyword(s):  
Plant Height ◽  
Yield Components ◽  
Seed Oil ◽  
Tap Water ◽  
Dry Weight ◽  
Salinity Level ◽  
Shoot Dry Weight ◽  
Weight Yield ◽  
Yield And Yield Components ◽  
Salinity Levels

<p class="emsd-body"><span lang="EN-GB">Seeds of ten sesame cultivars (Karaj, Darab, Safiabad, Jiroft, Borazjan, Yellow-white, Felestin, Ultan, Isfahan and Abpakhsh) were sown into soil filled pots in 2008 and 2009. Pots were watered with six levels of salts (0.0038 (tap water as control), 4.89, 8.61, 10.5, 14.54, 17.74 ds.m<sup>-1</sup> NaCl) until full maturity. Plant height, root and shoot dry weight, yield and yield components, seed oil and protein contents of cultivars were measured. Increasing salinity caused significant reduction in plant height, root and shoot dry weight, yield and yield components, seed oil and protein contents of all cultivars. However, there were significant differences among the cultivars for measured traits for each salinity level. Based on seed oil yield data, Safiabad and Kraj at 0.0038ds.m<sup>-1</sup>, Safiabad and Ultan at 4.89ds.m<sup>-1</sup>, Ultan, Safiabad and Darab at 8.61 salinity levels were the superior cultivars. High variability in tolerance to salinity among the tested sesame cultivars suggests that selection of more salt tolerant cultivars for planting or breeding purposes is possible.</span></p><p class="emsd-body"><span lang="EN-GB">Highlights</span></p><p class="emsd-body"><span lang="EN-GB">Effects of contrasting salinity levels (0.0038 (tap water as control), 4.89, 8.61, 10.5, 14.54, 17.74 ds.m<sup>-1</sup>NaCl) on sesame cultivars were tested. Salinity reduced plant growth and yield and seed oil and protein contents. However, there were significant differences among the cultivars for measured traits for each salinity level. </span></p>

scholarly journals EFEKTIFITAS FUNGI MIKORIZA ARBUSKULA (FMA) SERTA PUPUK NITROGEN DAN FOSFAT TERHADAP PERTUMBUHAN SEMAI JABON (Anthocephallus cadamba Roxb.) Effectiveness of Arbuscula Mycorrhizal Fungi (AMF) Nitrogen and Phosphate Fertilizer toward Jabon ........

2017 ◽  
Vol 8 (1) ◽  
pp. 20-25
Author(s):  
Fransisca Yosina Regina Luturmas ◽  
Sri Wilarso Budi R. ◽  
Irdika Mansur
Keyword(s):  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Dry Weight ◽  
Phosphate Fertilizer ◽  
Soil Types ◽  
Shoot Dry Weight ◽  
Main Plot ◽  
Fast Growing Species ◽  
Glomus Sp ◽  
Plot Design

Jabon (Anthocephallus cadamba Roxb.) is one of fast-growing species with high economical value, and well adapted on some of soil types. The aims of this research were to analyze the effectiveness of AMF species isolated from Samama (Anthocephallus macrophyllus (Roxb.) Havil) and to determine the favorable dose of nitrogen and phosphate fertilizer for Jabon growth. The research was conducted CRD-split plot design main plot was Acaulospora sp.1 (M1), Glomus sp.1 (M2), Acaulospora sp.2 (M3), Acaulospora sp.3 (M4), Glomus sp.2 (M5). While the fertilizer as the sub-plot, consist of control (P0), urea 0.5g+rockphosphat 2g (P1), urea 1g+rockphosphat 4g (P2). The result showed that AMF and fertilizer applications effectively improved Jabon grown, especially in height, stem diameter, and shoot dry weight. The interaction (M4P1) increased plant height 154.73%, diameter 75.38% and shoot dry weight 376.09% compared with control. P1 treatment was better for growth of Jabon that was inoculated by mycorrhiza. Acaulospora sp.1 (M1) originally from Samama without fertilizer had the best effectiveness for Jabon growth.Key words: Acaulospora, indigenous

scholarly journals MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Ana Cristina Portugal Pinto De Carvalho ◽  
Fabrina Bolzan Martins
Keyword(s):  
Plant Height ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Salt Mixture ◽  
Musa Spp ◽  
Fresh Biomass ◽  
Shoot Dry Weight ◽  
Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

HEIGHT CONTROL OF POT CHRYSANTHEMUMS WITH PRE- AND POST-PLANT TREATMENTS OF DAMINOZIDE AND UNICONAZOLE

1990 ◽  
Vol 70 (3) ◽  
pp. 925-930 ◽  
Author(s):  
PETER R. HICKLENTON
Keyword(s):  
Plant Height ◽  
Foliar Spray ◽  
Dry Weight ◽  
Chrysanthemum Morifolium ◽  
Growth Retardants ◽  
Dendranthema Grandiflora ◽  
Height Control ◽  
Shoot Dry Weight ◽  
Butanedioic Acid ◽  
Higher Doses

This study investigated the effects of growth retardants uniconazole [(E) - (p-chlorohenyl) -4, 4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol] and daminozide (butanedioic acid mono 2,2-dimethylhydrazide) on three chrysanthemum (Dendranthema grandiflora Tzvelev.) cultivars. Uniconazole applied as a soil drench (0.02 mg a.i. pot−1) or foliar spray (0.014 mg a.i. pot−1) 10 d after removal of the shoot tip reduced plant height at harvest in cultivars Deep Luv, Tip and Tara. Higher doses of uniconazole resulted in further plant height reduction in Tip and Tara but not in Deep Luv. Daminozide spray (14 mg a.i. pot) and uniconazole spray (0.028 or 0.056 mg a.i. pot−1) produced plants of similar height. Pre-plant dips of both growth retardants were less effective than sprays or drenches in controlling height. Flower area and flower dry weight were reduced with uniconazole drench and spray, and daminozide spray in each cultivar. Shoot dry weight was similarly affected in Tip and Tara but not in Deep Luv. Flowering was delayed in each cultivar by post-plant treatments of uniconazole drench and spray and by daminozide spray (0.08, 0.014 and 14 mg a.i. pot−1, respectively), and by daminozide and uniconazole pre-plant dips (5.0 mg L−1 and 4000 mg L−1, respectively).Key words: Sumagic, XE-1019, B-Nine, Alar, Chrysanthemum × morifolium, Dendanthema grandiflora

scholarly journals Grafted eggplant yield, quality and growth in infested soil with Verticillium dahliae and Meloidogyne incognita

2009 ◽  
Vol 44 (12) ◽  
pp. 1673-1681 ◽  
Author(s):  
Sebahattin Çürük ◽  
H. Yıldız Dasgan ◽  
Sedat Mansuroğlu ◽  
Şener Kurt ◽  
Meltem Mazmanoğlu ◽  
...  
Keyword(s):  
Plant Height ◽  
Verticillium Dahliae ◽  
Meloidogyne Incognita ◽  
Solanum Melongena ◽  
Dry Weight ◽  
Fruit Weight ◽  
Infested Soil ◽  
Solanum Torvum ◽  
Shoot Dry Weight ◽  
Soil Infestation

The objective of this work was to evaluate the effect of grafting (onto Solanum torvum Sw.) on plant growth, yield and fruit quality of the Pala and Faselis eggplant (Solanum melongena L.) cultivars, grown in a soil infested with Verticillium dahliae Kleb. and Meloidogyne incognita, or in noninfested soil. Soil infestation decreased yield, plant height, final above-ground biomass, and also reduced fruit mean weight and shoot dry weight depending on cultivar or grafting. Grafting decreased fruit oxalic acid and the soluble solid contents, and increased mean fruit weight, depending on cultivar and soil infestation. Grafting also reduced the negative effects of the pathogens on disease index, plant height and shoot dry weight. Cultivar Pala was more vigorous than Faselis, and S. torvum was a vigorous rootstock. The combination of a vigorous rootstock with a weak cultivar (Faselis) is more profitable than that of a vigorous rootstock and a vigorous cultivar (Pala). Using S. torvum as a rootstock for cultivar Faselis, grown in soil infested with the pathogens, is most likely to be useful in conventional and low-input sustainable horticulture, since grafting increases protection against the pathogens, and reduces the losses in quality and yield.

scholarly journals Formate-Dependent H2 Production by the Mesophilic Methanogen Methanococcus maripaludis

2008 ◽  
Vol 74 (21) ◽  
pp. 6584-6590 ◽  
Author(s):  
Boguslaw Lupa ◽  
Erik L. Hendrickson ◽  
John A. Leigh ◽  
William B. Whitman
Keyword(s):  
Dry Weight ◽  
Maximal Activity ◽  
Growth Conditions ◽  
H2 Production ◽  
Resting Cells ◽  
Wild Type ◽  
Methanococcus Maripaludis ◽  
Gene Encoding ◽  
Major Pathway ◽  
Key Enzyme

ABSTRACT Methanococcus maripaludis, an H2- and formate-utilizing methanogen, produced H2 at high rates from formate. The rates and kinetics of H2 production depended upon the growth conditions, and H2 availability during growth was a major factor. Specific activities of resting cells grown with formate or H2 were 0.4 to 1.4 U�mg−1 (dry weight). H2 production in formate-grown cells followed Michaelis-Menten kinetics, and the concentration of formate required for half-maximal activity (Kf ) was 3.6 mM. In contrast, in H2-grown cells this process followed sigmoidal kinetics, and the Kf was 9 mM. A key enzyme for formate-dependent H2 production was formate dehydrogenase, Fdh. H2 production and growth were severely reduced in a mutant containing a deletion of the gene encoding the Fdh1 isozyme, indicating that it was the primary Fdh. In contrast, a mutant containing a deletion of the gene encoding the Fdh2 isozyme possessed near-wild-type activities, indicating that this isozyme did not play a major role. H2 production by a mutant containing a deletion of the coenzyme F420-reducing hydrogenase Fru was also severely reduced, suggesting that the major pathway of H2 production comprised Fdh1 and Fru. Because a Δfru-Δfrc mutant retained 10% of the wild-type activity, an additional pathway is present. Mutants possessing deletions of the gene encoding the F420-dependent methylene-H4MTP dehydrogenase (Mtd) or the H2-forming methylene-H4MTP dehydrogenase (Hmd) also possessed reduced activity, which suggested that this second pathway was comprised of Fdh1-Mtd-Hmd. In contrast to H2 production, the cellular rates of methanogenesis were unaffected in these mutants, which suggested that the observed H2 production was not a direct intermediate of methanogenesis. In conclusion, high rates of formate-dependent H2 production demonstrated the potential of M. maripaludis for the microbial production of H2 from formate.

scholarly journals Simulated mesotrione drift followed by glyphosate, imazethapyr, bentazon or glyphosate plus chlorimuron in soybean

2009 ◽  
Vol 89 (2) ◽  
pp. 265-272
Author(s):  
L. R. Brown ◽  
D. E. Robinson ◽  
K. Chandler ◽  
C. J. Swanton ◽  
R. E. Nurse ◽  
...  
Keyword(s):  
Glycine Max ◽  
Key Words ◽  
Plant Height ◽  
Seed Yield ◽  
Plant Density ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Glycine Max L ◽  
Herbicide Drift

There have been anecdotal accounts of increased crop sensitivity due to herbicide drift followed by an in-crop herbicide. An experiment was conducted from 2005 to 2007 at Elora, Ridgetown, and Woodstock, Ontario, to determine the effects of simulated mesotrione drift followed by in-crop applications of glyphosate, imazethapyr, bentazon and glyphosate plus chlorimuron on glyphosate-resistant soybean [Glycine max (L.) Merr.] visual injury, plant height, plant density, shoot dry weight, and seed yield. As the rate of simulated mesotrione drift increased, there was an increase in soybean injury and a decrease in shoot dry weight, height, and yield. Simulated mesotrione drift followed by bentazon resulted in synergistic responses in injury shortly after application in some environments. This increase in injury was transient, with no synergistic responses in density, shoot dry weight, and yield. In contrast, antagonistic responses were observed when glyphosate, imazethapyr, or glyphosate plus chlorimuron were applied after simulated mesotrione drift in some environments. Further research is required to develop a better understanding of the interactions of drift followed by the application of an in-crop herbicide. Key words: Bentazon, chlorimuron, glyphosate, imazethapyr, mesotrione, synergism

Identification of a system that allows a Rhizobium tropici dctA mutant to grow on succinate, but not on other C4-dicarboxylates

2001 ◽  
Vol 47 (6) ◽  
pp. 509-518 ◽  
Author(s):  
Silvia Batista ◽  
Ana I Catalán ◽  
Ismael Hernández-Lucas ◽  
Esperanza Martínez-Romero ◽  
O Mario Aguilar ◽  
...  
Keyword(s):  
Transport System ◽  
Biochemical Characterization ◽  
Dry Weight ◽  
Acetylene Reduction Activity ◽  
Insertion Mutant ◽  
Rhizobium Tropici ◽  
Gene Encoding ◽  
Shoot Dry Weight ◽  
Reduction Activity ◽  
Bean Plants

A defined insertion mutant of a gene encoding a homolog of the rhizobial C4-dicarboxylate permease (dctA) was constructed in Rhizobium tropici strain CIAT899. This mutant (GA1) was unable to grow on fumarate or malate; however, in contrast with other rhizobial dctA mutants, it retained a limited ability to grow on succinate with ammonia as a nitrogen source. Our results suggest the presence of a novel succinate-specific transport system in R. tropici. Biochemical characterization indicated that this alternative transport system in GA1 is active and dependent on an energized membrane. It was also induced by succinate and aspartate, and was repressed by glucose and glycerol. Bean plants inoculated with GA1 showed a reduced nitrogen-fixing ability, achieving only 29% of the acetylene reduction activity determined in CIAT899 strain nodules, 33 days after inoculation. Also, bean plants inoculated with GA1 had reduced shoot dry weight compared with plants inoculated with the wild-type strain.Key words: succinate transport, Rhizobium tropici, C4-dicarboxylate uptake.

scholarly journals Effect of water hyacinth compost on the morpho-physiological parameters of Soybean (Glycine max l.)

2021 ◽  
Vol 2 (1) ◽  
pp. 084-095
Author(s):  
Ogbuehi HC ◽  
Ibe PK
Keyword(s):  
Glycine Max ◽  
Leaf Area ◽  
Plant Height ◽  
Water Hyacinth ◽  
Seed Weight ◽  
Dry Weight ◽  
Effect Of Water ◽  
Shoot Dry Weight ◽  
Glycine Max L ◽  
Number Of Leaves

A pot experiment was conducted under rainfed condition to study the effect of water hyacinth compost on the morpho-physiological parameters of soybean (Glycine max L.) at the Teaching and Research Farm of Faculty of Agriculture and Veterinary Medicine, Imo State University, Owerri. The treatments were control (T1) 100g (T2), 150g (T3) and 200g (T4) of water hyacinth compost and replicated four times. The treatments were arranged in Complete Randomized Design (CRD). The parameters measured were plant height, number of leaves, leaf area (cm2), leaf area index, relative growth rate (RGR), Net assimilation rate (NAR), shoot dry weight(g), yield and yield components (Number of pods, pods weight, 100 seed weight). The results obtained indicated that T3 significantly produced highest plant height (57.6cm) compare to control. While it was observed that T4 (200g) significantly produced the highest number of leaves (233.25), leaf area (631.80cm2), shoot dry weight (15.445g), number of pods (129.75), pod weights (25.38g) seed weight (7.23g) and yield (0.72kg/ha) relative to control and other treatment levels. Root parameters were also significantly improved by the rates of water hyacinth application compared to control. It will be worthy to note that there was no nodulation perhaps that was why the yield was poor. The results showed that soybean growth can effectively be improved with incorporation of water hyacinth into soil.

scholarly journals Moisture Stress: An Alternative Method of Height Control to B-nine (daminozide)

1992 ◽  
Vol 10 (4) ◽  
pp. 232-235
Author(s):  
David R. Brown ◽  
D. Joseph Eakes ◽  
Bridget K. Behe ◽  
Charles H. Gilliam
Keyword(s):  
Plant Height ◽  
Control Method ◽  
Dry Weight ◽  
Moisture Stress ◽  
Node Number ◽  
Height Control ◽  
Shoot Dry Weight ◽  
Bedding Plant ◽  
Medium Type ◽  
Pepper Plants

Abstract Moisture stress was compared to B-nine (daminozide) as a method of height control for annual bedding plant transplants. Three plant species, ‘Big Boy’ tomato, ‘California Wonder’ pepper and ‘Janie Gold’ marigold, were grown in 132 cm3 (8.05 in3) cell packs containing one of 2 commercial media, Fafard #3 or Pro-Mix BX. Treatments included moisture stress (MS), 2 concentrations of B-nine (2500 ppm applied twice and 5000 ppm once), and an untreated control. Method of height control and medium type had an interactive influence on height for each of the 3 species. Moisture stress tomato and marigold were shorter in the Fafard #3 medium compared to those in the Pro-Mix BX medium. Regardless of medium, MS tomato and marigold transplants were shorter or similar in size to the most effective B-nine treatment, 2500 ppm applied twice. Moisture stress and the B-nine treatments for pepper plants grown in the Fafard #3 medium reduced plant height similarly compared to the controls. However, when pepper plants were grown in the Pro-Mix BX medium, only B-nine treatments reduced plant height compared to the controls. Treatments producing short plants did not reduce node number, hence plants appeared fuller than treatments with tall plants. Shoot dry weights for MS tomato and marigold were less than those of plants receiving the other height control treatments, regardless of medium type. Plants of all 3 species grown in the Fafard #3 medium had less shoot dry weight than Pro-Mix BX plants across the 4 height control treatments.

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