scholarly journals 091 Cytogenetics of Inland Saltgrass

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 457B-457
Author(s):  
Scott Reid ◽  
Judy Harrington ◽  
Harrison Hughes
Keyword(s):  
Chromosome Number ◽  
Seed Production ◽  
Chromosome Numbers ◽  
Seed Set ◽  
Distichlis Spicata ◽  
Root Tip ◽  
Limiting Factor ◽  
Good Seed ◽  
Poor Seed

Inland saltgrass, Distichlis spicata var. stricta (Torr.) Beetle, is a native western U.S. grass that has potential in turf situations. Elite clones with outstanding potential have been selected. Poor seed production appears to be the main limiting factor for its use as turf in saline conditions. In order to better understand seed set, 40 genotypes were examined for chromosome number and morphology. Chromosome numbers of root tip metaphase spreads varied with the most common being 2n = 38. However, chromosome numbers of 39, 40, 42, and 74 were also observed. Meiotic examination of anthers revealed 19 bivalents for those with 38 chromosomes while those with 42 had 20 bivalents with 2 unpaired chromosomes. The unpaired chromosomes lagged at anaphase. Crosses among genotypes of different chromosome number have resulted in good seed set. Harvested seed are germinable and plants from these crosses are being grown for further studies.

Izmir subterranean clover (Trifolium subterraneum L. var. subterraneum)

2007 ◽  
Vol 47 (2) ◽  
pp. 226 ◽  
Author(s):  
P. G. H. Nichols ◽  
G. A. Sandral ◽  
B. S. Dear ◽  
C. T. de Koning ◽  
D. L. Lloyd ◽  
...  
Keyword(s):  
Seed Production ◽  
Seed Set ◽  
New South ◽  
South Australia ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Good Seed ◽  
Improvement Program ◽  
South Wales ◽  
Pasture Legume

Izmir is a hardseeded, early flowering, subterranean clover of var. subterraneum (Katz. et Morley) Zohary and Heller collected from Turkey and developed by the collaborating organisations of the National Annual Pasture Legume Improvement Program. It is a more hardseeded replacement for Nungarin and best suited to well-drained, moderately acidic soils in areas with a growing season of less than 4.5 months. Izmir seed production and regeneration densities in 3-year pasture phases were similar to Nungarin in 21 trials across southern Australia, but markedly greater in years following a crop or no seed set. Over all measurements, Izmir produced 10% more winter herbage and 7% more spring herbage than Nungarin. Its greater hardseededness and good seed production, makes it better suited to cropping rotations than Nungarin. Softening of Izmir hard seeds occurs later in the summer–autumn period than Nungarin, giving it slightly greater protection from seed losses following false breaks to the season. Izmir is recommended for sowing in Western Australia, New South Wales, Victoria, South Australia and Queensland. Izmir has been granted Plant Breeders Rights in Australia.

scholarly journals Developmental sequence of chromosome number in a cytologically unstable Rubus hybrid

1961 ◽  
Vol 2 (1) ◽  
pp. 10-24 ◽  
Author(s):  
G. Haskell ◽  
N. N. Tun
Keyword(s):  
Chromosome Number ◽  
Virus Infection ◽  
Chromosome Numbers ◽  
Mother Cell ◽  
Root Tip ◽  
Developmental Sequence ◽  
General Increase ◽  
Incremental Change ◽  
Rubus Species

1. Serial cytological observations were made on one abnormal seedling from the thornless segregants of the F2 progeny from crossing two tetraploid (2n = 28) Rubus species. The number of root-tip chromosomes was found to vary between 9 and 46 per cell, with the mode always at 35. The wide variation in chromosome number decreased with time, until there was almost stability at 2n = 35. A vegetative (stipule) cell was 2n = 39. The numbers lower than 35 were not due to terminal fusion.2. Evidence from the number of satellites per cell suggests that not always were the same chromosomes being eliminated, although there is a general increase in satellite number with increased numbers of chromosomes per cell.3. There was a correlation between the chromosome numbers and corresponding cell sizes within the aneuploid series. This may be associated with the incremental change of DNA following the addition or subtraction of individual chromosomes, which are highly similar in Rubus.4. The cause of the instability was not definitely established. Its initiation is probably attributable to the egg mother cell, as Rubus pollen reacts sensitively to chromosome unbalance. No abnormalities were observed in the mitoses, and virus infection was not responsible for the instability.5. Chromosome number instability of this plant is discussed in relation to other examples from the same and different genera: it is suggested that the changes in chromosome number known in various Rubi may have arisen somatically.

STUDIES ON THE CYTOLOGY OF BROMEGRASS, BROMUS INERMIS LEYSS.

1958 ◽  
Vol 38 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Björn Sigurbjörnsson ◽  
Akira Mochizuki ◽  
John D. Truscott
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Somatic Chromosome ◽  
Seed Set ◽  
Bromus Inermis ◽  
Somatic Chromosome Number ◽  
Simultaneous Selection ◽  
Selection For ◽  
Type Strains ◽  
Selection Of

A population of 222 plants of 16 varieties and strains of northern and southern bromegrass was studied for somatic chromosome number. The following observations were noted: Aneuploid plants, including the chromosome numbers 54, 55, 57 and 58, were found within the species, Bromus inermis. One plant was found to have 49 chromosomes. A cytological difference was found between certain varieties of northern and southern types of bromegrass. The northern type had a significantly higher number of aneuploid plants with chromosome numbers in excess of 56, while the southern type had more aneuploid plants with fewer than 56 chromosomes. Some evidence was found suggesting that selection of northern type strains may bring about a simultaneous selection for additional chromosomes, whereas selection for southern type strains may involve a simultaneous selection for plants with missing chromosomes. Average seed set was found to be lowered in the presence of two additional chromosomes or absence of two chromosomes from the euploid complement.

scholarly journals Production of Interspecific Hybrids between Hexaploid Fragaria moschata and the diploid species F. nubicola and F. viridis.

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 610b-610
Author(s):  
Robert H. Bors ◽  
J. Alan Sullivan
Keyword(s):  
Seed Germination ◽  
Ploidy Level ◽  
Interspecific Hybrids ◽  
Leaf Morphology ◽  
Seed Set ◽  
Embryo Rescue ◽  
Diploid Species ◽  
Interspecific Crosses ◽  
Good Seed ◽  
Poor Seed

Interspecific crosses with Fragaria moschata (6x) have been hampered by ploidy level differences, poor seed set, and extremely poor seed germination. Modification of pollination practices, embryo rescue, and use of several genotypes has allowed over 80 synthetic tetraploids to be created from 14 cross combinations. Germplasm for the experiment consisted of eight selections of F. moschata (6x), two of F. nubicola (2x), and two of F. viridis (2x). Both 2x × 6x and 6x × 2x crosses were performed. Initially, negligible seed set occurred on F. nubicola and F. viridis when multiple flowers per truss were pollinated. When only one cross was performed per truss, with other flowers removed, seed set was greatly enhanced. F. moschata was much more tolerant of multiple crosses per truss. The crossing combination of F. moschata × F. nubicola gave the worst seed production. Other species combinations were capable of producing good seed set with noticeable differences between individual selections. When achenes were halved, only 1% appeared normal, 2% were underdeveloped or shrunken, the remainder were empty. Many of the malformed and most of the normal embryos germinated using the cut achene method. Achenes were surface-sterilized, cut in half, and placed on MS media with activated charcoal (3g·L–1), sucrose (30g·L–1), and no hormones. Germination occurred only from achenes from fully ripened fruit. Viable hybrids were obtained from 2x × 6x as well as 6x × 2x crosses. Fragaria viridis–F. moschata hybrids closely resembled F. moschata while F. nubicola–F. moschata hybrids were more intermediate in leaf morphology.

Apomixis in diploid × triploid Tripsacum dactyloides hybrids

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 528-532 ◽  
Author(s):  
R. A. Sherman ◽  
P. W. Voigt ◽  
B. L. Burson ◽  
C. L. Dewald
Keyword(s):  
Chromosome Number ◽  
Ploidy Level ◽  
Chromosome Numbers ◽  
Germ Plasm ◽  
Seed Set ◽  
Tripsacum Dactyloides ◽  
Mode Of Reproduction ◽  
Selected Subset ◽  
Diploid Level ◽  
High Level

Apomixis can hinder plant improvement if sexual or apomictic germ plasm of the appropriate ploidy level, required for effective manipulation of apomixis, is not available. To develop needed germ plasm and increase knowledge of apomixis in Tripsacum dactyloides, diploid (2n = 2x = 36) sexual plants were crossed with a highly apomictic triploid (2n = 3x = 54) accession in an attempt to transfer apomixis from a polyploid to the diploid level. The fertility of most hybrids was very low, 69% had 10% or less seed set. A selected subset of 48 hybrids, including most of the more fertile plants, was studied cytologically. The chromosome number of these hybrids ranged from 2n = 36 to 2n = 54. All but 3 of 46 of the 48 hybrids showed indications of apomictic development. Those with near-diploid chromosome numbers were primarily sexual. Highly apomictic hybrids, based on percent diplosporous ovules, had 43 or more chromosomes. Fertility of these hybrids was not related to chromosome number. Fertility of sexual to moderately apomictic plants was reduced as chromosome number increased. Apomixis in T. dactyloides is facultative. Backcrosses are needed to develop germ plasm with a high level of apomixis and near-diploid chromosome numbers.Key words: eastern gama-grass, chromosome number, fertility, mode of reproduction, germ plasm.

FLORAL INITIATION AND SEED PRODUCTION IN FESTUCA SCABRELLA TORR.

1967 ◽  
Vol 47 (5) ◽  
pp. 577-583 ◽  
Author(s):  
A. Johnston ◽  
M. D. MacDonald
Keyword(s):  
Seed Production ◽  
Shoot Apex ◽  
Floral Development ◽  
Seed Set ◽  
Growth Habit ◽  
Floral Initiation ◽  
Developmental Morphology ◽  
Seed Crop ◽  
Factors Associated ◽  
Poor Seed

Three thousand seven hundred and eighty shoot apices of Festuca scabrella Torr. were examined over a three-year period. Stages of development and height of apices above the root-stem transition were recorded. Initiation and partial floral development occurred in autumn, 1963 and 1965; seed set in native stands was good in 1964 and 1966. Initiation failed to occur in 1964 and 1966; seed set was poor in 1965 and a poor seed crop in 1967 appeared likely. Causes of induction and later initiation of floral development were not determined. The tendency of F. scabrella to decrease in abundance when grazed heavily was attributed to its erect growth habit and lack of lateral tillers rather than to factors associated with the developmental morphology of the shoot apex.

Cytogenetic and Pollination Studies in the Genus Verticordia DC

1991 ◽  
Vol 39 (3) ◽  
pp. 261 ◽  
Author(s):  
AP Tyagi ◽  
J Mccomb ◽  
J Considine
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Seed Set ◽  
Interspecific Crosses ◽  
Root Tips ◽  
Chromosome Counts ◽  
Flower Buds ◽  
Taxonomic Distance ◽  
Self Compatibility ◽  
High Level

Aspects of the genetic and physiological barriers to hybridisation have been assessed for the genus Verticordia which contains many species with significant potential for ornamental horticulture. The chromosome numbers of 52 species were analysed using flower buds and root tips. Previously published reports on chromosome numbers were confirmed for 21 species and new numbers reported in a further 31. Self compatibility was present in 20 species and only three species were shown to possess barriers to self pollination. Seed set occurred in two of six interspecific crosses carried out within sections. In 21 wider crosses the degree of pollen tube development was increasingly repressed with taxonomic distance. The chromosome counts also support the reclassification of the genus Verticordia into three subgenera: Chrysoma, Verticordia and Eperephes. Species within each section of a subgenus generally had the same chromosome number. Exceptions occurred in the subgenus Verticordia, sections Verticordia, Intricata and Micrantha. In species with one or two ovules, the chromosome number was also low (6, 7, 8 or 9) while species with six or more ovules had a higher chromosome number (11 or 22). There was, however, no relationship between chromosome number and chromosome size, nor between chromosome number and any observed feature which might be attributable to polyploidy or allopolyploidy. With the possible exception of V. grandis, which displayed loss of seasonality in flowering, all putative polyploids behaved developmentally as diploids and displayed a high level of male fertility.

REDUCTION OF CHROMOSOME NUMBER IN ROOT TIP CELLS OF MEDICAGO

1974 ◽  
Vol 16 (1) ◽  
pp. 219-227 ◽  
Author(s):  
R. S. Sadasivaiah ◽  
K. Lesins
Keyword(s):  
Chromosome Number ◽  
Ploidy Level ◽  
Chromosome Numbers ◽  
Plant Evolution ◽  
Root Tip ◽  
Aneuploid Chromosome ◽  
Tip Cells ◽  
Root Tip Cells ◽  
In Cells

Cytological studies on progenies obtained from intercrosses between colchicine-induced octoploid (2n = 8x = 64) plants of alfalfa showed normal, haploid and aneuploid chromosome numbers in cells of the same root tip. Critical observations on cells at different divisional stages revealed the occurrence of meiosis-like mitotic divisions, resulting in a reduction of chromosome number. The frequency of cells showing reductional divisions appeared to vary with the ploidy level of the material. The possible significance of chromosome reduction in plant evolution is indicated.

scholarly journals New Contribution to the Knowledge on the Chromosome Numbers of Turkish Cerambycidae (Coleoptera)

2021 ◽  
Vol 69 (2) ◽  
pp. 93-99
Author(s):  
Yavuz Koçak ◽  
Elmas Yağmur
Keyword(s):  
Chromosome Number ◽  
Sex Determination ◽  
Chromosome Numbers ◽  
Chromosome Count ◽  
Chromosomal Number ◽  
The Poor ◽  
Karyological Data ◽  
First Time

Information on the karyotypes of Turkish species of Cerambycidae is scanty. Our study contributes to the knowledge of the karyological data (chromosomal number and mechanism of sex determination) of five Turkish longicorn beetles; karyotypes of four taxa, one endemic, are described for the first time and for the remaining one, Purpuricenus budensis (Götz, 1783), the previously published chromosome count is confirmed. The chromosome number of Purpuricenus desfontainii inhumeralis Pic, 1891 and Purpuricenus budensis (Götz, 1783) (Cerambycinae, Trachyderini) was found to be 2n = 28 (13 + Xyp); Clytus rhamni Germar, 1817 and Plagionotus floralis (Pallas, 1773) (Cerambycinae, Clytini) 2n = 20 (9 + Xyp); and the endemic Dorcadion triste phrygicum Peks, 1993 (Lamiinae, Dorcadionini) 2n = 24 (11 + Xyp). In view of the paucity of data available until now, our study is important for both to improve the poor karyological knowledge of Turkish Cerambycidae and to provide an incentive for other researchers.

scholarly journals Karyotype Analysis of Alnus Mill. (Betulaceae) Species Originating From Northeastern Asia

2010 ◽  
Vol 59 (1-6) ◽  
pp. 219-223 ◽  
Author(s):  
Liu Jun ◽  
Ren Bao-Qing ◽  
Luo Peigao ◽  
Ren Zhenglong
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Karyotype Analysis ◽  
Full Potential ◽  
Northeastern Asia ◽  
Ploidy Levels ◽  
Genetic Makeup ◽  
Fossil Records

Abstract Alnus Mill. (alder) is an ecologically valuable tree genus. It is essential to study its genetic makeup in order to use alder trees to their full potential. Five specimens from four Alnus species (A. mandshurica, A. pendula, A. sibirica, and A. sieboldiana), found in northeastern Asia, were subjected to karyotype analysis. The analysis showed that these tree samples could be divided into three categories based on chromosome numbers or ploidy levels: viz., 2n = (4x) = 28, 2n = (8x) = 56 and 2n = (16x) = 112. The differences in chromosome number and karyotype parameters among Alnus species and even within the same species possibly resulted from natural polyploidization. Comparing the chromosome numbers of Alnus species in China with those in Japan showed that there appear to be only two categories in China, whereas there are up to five categories in Japan. The earliest fossil records of Alnus pollen were also discovered in Japan. We conclude that the center origin of Alnus spp. is Japan rather than China.

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