scholarly journals 315 Alstroemeria Plants Free of Alstroemeria Mosaic Potyvirus (AIMN) through in Vitro Culture Shoots and Thermotherapy

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 446C-446
Author(s):  
Lourdes Cervantes-Dl̀az ◽  
Emma Zavaleta-Mejl̀a ◽  
Alejandra Gutièrrez-Espinosa ◽  
J. Antonio Santizo-Rincan
Keyword(s):  
In Vitro Culture ◽  
Cut Flowers ◽  
Indole Butyric Acid ◽  
Cytoplasmic Inclusions ◽  
Virus Particles ◽  
Murashige Skoog ◽  
Isopentenyl Adenine ◽  
Propagation Methods ◽  
Viral Inclusions

Alstroemeria (Alstroemeria spp.) is cultivated for cut flowers. Traditional propagation methods are by division of rhizomes from mature plants, so that viruses occurring in the crop can be multiplied and cause a decrease in the quality and production. The objective of this work was to obtain Alstroemeria cv. Rosario plants free of Alstromeria Mosaic Potyvirus (AlMV) by in vitro culture of shoots and thermotherapy. The best percentage of explants without contamination was obtained when adding the disinfectant PPM (1%) to the medium Murashige-Skoog (MS) while the best induction of buds was obtained when using explants of 1.5 cm. in length. In vitro multiplication of shoots was best in treatments with 2iP (isopentenyl adenine), BA (benzyladenine), and zeatin (4.4, 6.1, and 6.6 buds per explant, respectively). Rhizogenesis was observed in rhizomes growing in MS with 4.9 μM AIB (indole butyric acid) and 1.5 g·L-1 of sugar. Sixty-seven percent of plants growing in vitro did not react to AlMV antiserum and did not show particles and viral inclusions. Thermotherapy treatments of 45, 50, and 55 °C during different periods of time produced from 25% to 87.5% of plants that did not react to AlMV antiserum and did not show virus particles or cytoplasmic inclusions.

scholarly journals An Electron Microscope Study of Polyoma Virus in Hamster Kidney

1960 ◽  
Vol 7 (4) ◽  
pp. 753-760 ◽  
Author(s):  
Allan F. Howatson ◽  
June D. Almeida
Keyword(s):  
Electron Microscope ◽  
Stromal Cells ◽  
Polyoma Virus ◽  
Tubular Structures ◽  
Cytoplasmic Inclusions ◽  
Virus Particles ◽  
Large Numbers ◽  
Mouse Cells ◽  
Newborn Animals

Electron microscope studies were made of hamster kidneys taken at daily intervals after injection of a variant of polyoma virus into newborn animals. Particular attention was paid to the period 5 to 6 days after injection at which time the necrotizing response was at its peak and virus particles were seen in greatest numbers. The most numerous particles were about 28 mµ in diameter. They were observed mainly within nuclei of stromal cells and are similar to the particles seen in large numbers in polyoma-infected mouse cells growing in vitro. They were not observed in cells of fully developed tumors. Filamentous or tubular structures closely associated with the 28 mµ particles and probably concerned in their formation are described. Considerable quantities of viral material were contained within cytoplasmic inclusions. In some of the inclusions larger particles of diameter 60 mµ were observed. The origin of these particles and their relation to the 28 mµ particles is discussed.

scholarly journals 883 PB 279 REGENERATING ADVENTITIOUS PLANTS FROM IN VITRO CULTURE OF LIATRIS SPICATA (L.) WILLD. COTYLEDONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560d-560
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Adventitious Shoot Formation ◽  
Murashige Skoog ◽  
Micropropagated Plants

Cotyledons from developing embryos 6 to 8 weeks old of Liatris spicata (blazing star) were cultured on Murashige-Skoog (MS) medium containing 0, 0.4, 4.4, and 44.4 μ M benzyladenine (BA) or 0, 0.2, 2.2, and 22.2 μ M thidiazuron (TDZ) to induce adventitious shoot formation. The highest percent of cotyledons forming shoots with highest shoot counts was on medium containing 2.2 μ M TDZ. Vitreous shoots formed on medium with 22.2 μ M TDZ. Callus derived from cotyledons and cultured on medium containing 4.44 μ M BA or 2.2 μ M TDZ formed adventitious shoots with highest shoot counts on 4.44 μ M BA. Adventitious shoots derived from cotyledons and callus were rooted on MS medium with 5.0 μ Mindole-3-butyric acid, acclimatized and grown ex vitro. All micropropagated plants appeared similar to each other.

scholarly journals ACCUMULATION OF CHEMICAL ELEMENTS IN TISSUES OF MEDICINAL PLANTS IN VITRO CULTURE

2020 ◽  
pp. 261-270
Author(s):  
Lina Viktorovna Zatonskaya ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Yekaterina Olegovna Kozlova ◽  
Viktor Anatol'yevich Petukhov
Keyword(s):  
Trace Elements ◽  
Medicinal Plants ◽  
In Vitro Culture ◽  
Nutrient Medium ◽  
Chemical Elements ◽  
Biologically Active ◽  
Accumulation Coefficient ◽  
Plant Materials ◽  
Murashige Skoog

Development of theoretical bases of optimization of the production process of biologically active substances in the cultivation of medicinal plants by modern methods of biotechnology is one of the main tasks of fundamental research in this area. The aim of this study was to study the content and features of the accumulation of elements in regenerants of medicinal plants grown on the nutrient medium Murashige-Skoog with different content of phytohormones. Regenerant plants in vitro culture actively accumulate elements (K, P, Ca, Mg, Na, Zn, Mn, Fe, B, Al, Cu, Pb, Sr, V, Cr, Ag, Mo, Ti, Ni, Co , Se, Cd). The content of the trace elements Mn, Zn and Cu that we studied was at the level of normal values for the vegetation of the continents, Fe in P. alba regenerants – much higher. The content of heavy and toxic metals Pb, Cd, As, Cr, Ni did not exceed the normal level in plants and the acceptable level for dietary supplements, plant-based tea, and medicinal plant materials. The high dependence of the accumulation of elements in plants-regenerants Iris spuria Art And Soul variety on the content of hormones in the nutrient medium was noted. Representatives of all three groups (macronutrients, trace elements and ultramicroelements) had a similar curve diagram. The maximum peak was located at a concentration of 2.5 µm BAP with auxins, then the coefficient decreased by 5 µm BAP, and the next lower peak was observed at 5.0 µm BAP+A. the Lowest value of KN was at a concentration of 7.5 µm BAP. The introduction of auxins at this BAP concentration stimulated another peak, and then the accumulation coefficient tended to increase at a concentration of 10.0 µm BAP. The revealed dependence makes possible the regulation and directed accumulation of elements necessary for the researcher in plant tissues.

scholarly journals Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Miguel Contreras Ramírez ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Contreras ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

scholarly journals Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions

2007 ◽  
Vol 59 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Tijana Cvetic ◽  
Aneta Sabovljevic ◽  
M. Sabovljevic ◽  
D. Grubisic
Keyword(s):  
In Vitro Culture ◽  
Callus Culture ◽  
Nutrient Medium ◽  
Culture Conditions ◽  
Prolonged Treatment ◽  
Gametophyte Development ◽  
Skoog Medium ◽  
Murashige Skoog ◽  
Half Strength

Pogonatum urnigerum (Polytrichaceae) in vitro culture was established from spores collected in nature. Both protonema and gametophore stages of gametophyte development were obtained. Also, a stable callus culture was established using hormone-free nutrient medium. The best nutrient medium for development was half-strength Murashige- Skoog medium supplemented with 1.5% sucrose. Auxin treatment enabled some gametophores to develop, but prolonged treatment induced early senescence. Tissues grown on cytokinin did not produce any gametophytes and did not survive prolonged treatment.

scholarly journals Morphogenesis in vitro Flower Pedicel of Lilium longiflorum with NAA and BAP

2020 ◽  
Author(s):  
Ni Kadek Dwipayani Lestari ◽  
Ni Wayan Deswiniyanti ◽  
Ida Astarini ◽  
Luh Made Arpiwi
Keyword(s):  
In Vitro Culture ◽  
Lilium Longiflorum ◽  
Growth Time ◽  
Cut Flowers ◽  
Flower Bud ◽  
Shoot Initiation ◽  
Best Response ◽  
Morphogenesis In Vitro ◽  
The Media

Lilies are one of the cut flowers most favored by consumers. Each lily plant can produce several flower stem, and each flower consists of many flower buds. Flower bud stems have the potential as explants in vitro culture. Propagation in vitro is a very promising technique for plant propagation. It has the advantage of being able to produce a lot of seedlings in a short time. Pedicel explants are reported to be the best explants after the petal to produce shoots and callus, but it is not yet known which pedicel age is best for explants. The aim of the study was to obtain information about the effect of NAA and BAP concentrations on Vacin & Went (VW) media and age of explant of flower stalks (pedicels) on morphogenesis of lilies in vitro. Method of this study is in vitro technique on VW media with complete randomized trial design. The results showed that explants of flower stems (pedicels) that were used in vitro culture were pedicel buds compared to pedicels with blooming flowers and withered flowers. Younger plants still have meristem tissue. The meristem tissue produces the auxin hormone which helps in cell division for faster bud growth The best concentrations produced in this study were 1 ppm NAA and BAP in percentage of growth, time of callus initiation, number of shoots, number of browning and browning time and 0,5 ppm NAA + 1 ppm BAP for time of shoot initiation. The result of 1 ppm NAA and BAP showed the best response because the composition of the media by using a combination of growth regulators from a group of auxins and cytokinin in a balanced amount can initiate cell enlargement and induction callus. Keywords: lily, pedicel, age, explant, stem

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