Root Cell Proliferation from Broccoli Root Tips in vitro Culture Using Indole Acetic Acid (IAA), Indole Butyric Acid (IBA) and Benzylaminopurine (BAP)

2016 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
A. Sharif Hossain ◽  
Nasir Ibrahim ◽  
Mohammed Aleissa
Keyword(s):  
Cell Proliferation ◽  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Indole Acetic Acid ◽  
Root Cell ◽  
Root Tips ◽  
Indole Butyric Acid

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

scholarly journals Effect of Hormones (IBA & IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach

2020 ◽  
Author(s):  
Javaid Iqbal ◽  
Bushra Khan ◽  
Sardar Khan ◽  
Nasreen Ghaffar ◽  
Ishaq Ahmad Mian ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Medicinal Plants ◽  
Butyric Acid ◽  
Indole Acetic Acid ◽  
Average Length ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Number Of Leaves ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

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