scholarly journals 340 The Plant Tissue Culture Information Exchange Media Database

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 450E-450
Author(s):  
April S. Herring ◽  
R. Daniel Lineberger
Keyword(s):  
Tissue Culture ◽  
Public Schools ◽  
Information Exchange ◽  
Web Site ◽  
Public Access ◽  
Tissue Cultures ◽  
User Input ◽  
Link Type ◽  
Micropropagated Plants ◽  
Types Of Information

The Univ. of Minnesota hosts the PLANT-TC Listserv as a service to the international tissue culture community (http://www.agro.agri.umn.edu/plant-tc/listserv/). One of the most frequently sought types of information is a recommendation for a “beginning point” for culturing a wide variety of plant species. Many of these inquiries come from individuals without ready access to extensive library holdings, including those in industry, public schools, and international sites. A Web site prototype that includes a searchable database of tissue culture recipes is being constructed and offered for user input. The database currently is located at http://webtutor.tamu.edu/students/herring/project/, but will be redirected to its own URL if user feedback is positive. The database also includes information about equipment and materials, media suppliers and domestic and foreign sources for tissue cultures and micropropagated plants. Other educational resources, including a virtual tour of a commercial tissue culture lab, are available on the site. The Web site and database will be reviewed by a panel of experts and modified according to their input prior to being posted for public access.

scholarly journals RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay

1986 ◽  
Vol 81 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Marilda M. Siqueira ◽  
Vanja Ferreira ◽  
Jussara P. Nascimento
Keyword(s):  
Tissue Culture ◽  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Bacterial Contamination ◽  
Virus Isolation ◽  
Rapid Diagnosis ◽  
Tissue Cultures ◽  
Virus Diagnosis ◽  
Under Five ◽  
Syncytial Virus

Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolaion in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the originl nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the call suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

scholarly journals “Stop Talking to People; Talk with Them”: A Qualitative Study of Information Needs and Experiences Among Genetic Research Participants in Pakistan and Denmark

2018 ◽  
Vol 14 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Zainab Afshan Sheikh ◽  
Klaus Hoeyer
Keyword(s):  
Qualitative Study ◽  
Information Exchange ◽  
Information Needs ◽  
Research Collaboration ◽  
Genetic Research ◽  
Research Participation ◽  
Research Participants ◽  
Mutual Engagement ◽  
Information Practices ◽  
Types Of Information

This article explores how research participants experienced information practices in an international genetic research collaboration involving the collection of biomaterial and clinical data in both Pakistan and Denmark. We investigated how people make sense of their research participation and the types of information they need and desire. We found great variation in what information exchange does and what participants experience as meaningful. For example, information practices could serve as a source of respect and recognition (in Denmark) or of hope, understanding or help when dealing with suffering (in Pakistan). Policies aimed at harmonizing ethics standards for international research do not encapsulate some of the most important aspects of information practices for the research participants involved. We suggest shifting the focus from standards of one-way information delivery to a more process-oriented form of research ethics, where the contextual exploration of local needs through a mutual engagement with participants gains more ground.

scholarly journals Micro-operations on cells in tissue cultures

1931 ◽  
Vol 109 (763) ◽  
pp. 380-403 ◽  
Keyword(s):  
Tissue Culture ◽  
Continuous Control ◽  
Tissue Cultures ◽  
Special Paper ◽  
Long Time

The application of the micromanipulative technique to the study of cells in tissue culture has for a long time offered an interesting, though difficult, field of research. Thus far, comparatively little has been done, the most notable contributions being those of Levi and of Peterfi and co-workers (Levi, 1926 ; Peterfi and Olivo, 1925 ; Peterfi, 1927 ; Peterfi and Kapel, 1928). Peterfi has also written a special paper on the technique (1927). The main deterrent in this work has been the lack of sufficient ease in the accurate and continuous control of the microneedles under the conditions required.

scholarly journals Sonication Culture of Antimicrobial Agent-Containing Cement Spacers Removed during Staged Revisions for Arthroplasty Infection

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Eric Gomez-Urena ◽  
Rafael J. Sierra ◽  
Kerryl E. Greenwood-Quiantance ◽  
Melissa J. Karau ◽  
James M. Steckelberg ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Antimicrobial Agent ◽  
Persistent Infection ◽  
Joint Infection ◽  
Revision Arthroplasty ◽  
Similar Proportion ◽  
Tissue Cultures ◽  
Periprosthetic Tissue ◽  
Prosthetic Joint ◽  
Sonication Culture

ABSTRACT Diagnosis of persistent infection at the time of reimplantation for staged revision of infected arthroplasties is challenging. Implant sonication culture for the diagnosis of prosthetic joint infection (PJI) has improved sensitivity compared to standard periprosthetic tissue culture. We report our experience with periprosthetic tissue culture and sonication culture of antimicrobial agent-containing cement spacers (ACSs) collected during second stages of staged revisions for arthroplasty infection. We studied 87 ACSs from 66 patients undergoing two-stage revision arthroplasty for PJI submitted for sonication culture, along with conventional periprosthetic tissue cultures. Two or more positive periprosthetic tissue cultures with the same organism were considered a positive tissue culture. For sonication culture, ≥20 CFU of bacteria per 10 ml of sonicate fluid was considered positive. The sensitivity and specificity of periprosthetic tissue and ACS sonication culture in detecting persistent infection, as well as their association with outcome, were assessed. Persistent infection occurred in 26% of cases. Periprosthetic tissue and sonicate fluid culture had specificities of 96.3 and 100% (P = 0.50), respectively, and sensitivities of 31.6 and 26.3% (P = 1.00), respectively, for the diagnosis of persistent infection. Thirteen subjects deemed not to have persistent infection at time of reimplantation and who had negative periprosthetic tissue and sonicate fluid cultures subsequently developed overt infection. Sonication culture of cement spacers identifies a similar proportion of patients with persistent infection during staged revisions, as detected by periprosthetic tissue cultures; both have low sensitivities to detect persistent infection.

scholarly journals The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2620
Author(s):  
Dmitry Miroshnichenko ◽  
Anna Klementyeva ◽  
Sergey Dolgov
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Copper Ions ◽  
Induction Medium ◽  
Tissue Cultures ◽  
Triticum Timopheevii ◽  
Dark Light ◽  
Green Plants ◽  
Callus Induction Medium

Triticum timopheevii Zhuk. is a tetraploid wheat that is utilized worldwide as a valuable breeding source for wheat improvement. Gene-based biotechnologies can contribute to this field; however, T. timopheevii exhibits recalcitrance and albinism in tissue cultures, making this species of little use for manipulation through genetic engineering and genome editing. This study tested various approaches to increasing in vitro somatic embryogenesis and plant regeneration, while reducing the portion of albinos in cultures derived from immature embryos (IEs) of T. timopheevii. They included (i) adjusting the balance between 2,4-D and daminozide in callus induction medium; (ii) cultivation using various darkness/illumination schedules; and (iii) inclusion of additional concentrations of copper ions in the tissue culture medium. We achieved a 2.5-fold increase in somatic embryogenesis (up to 80%) when 50 mg L−1 daminozide was included in the callus induction medium together with 3 mg L−1 2,4-D. It was found that the dark cultivation for 20–30 days was superior in terms of achieving maximum culture efficiency; moreover, switching to light in under 2 weeks from culture initiation significantly increased the number of albino plants, suppressed somatic embryogenesis, and decreased the regeneration of green plants. Media containing higher levels of copper ions did not have a positive effect on the regeneration of green plants; contrarily, the elevated concentrations caused albinism in plantlets. The results and relevant conclusions of the present study might be valuable for establishing an improved protocol for the regeneration of green plants in tissue cultures of T. timopheevii.

scholarly journals The impact of sonication cultures when the diagnosis of prosthetic joint infection is inconclusive

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0252322
Author(s):  
Taiana Cunha Ribeiro ◽  
Emerson Kiyoshi Honda ◽  
Daniel Daniachi ◽  
Ricardo de Paula Leite Cury ◽  
Cely Barreto da Silva ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Diagnostic Criteria ◽  
Gold Standard ◽  
Joint Infection ◽  
Clinical Criterion ◽  
Tissue Cultures ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
The Impact ◽  
Sonication Culture

Background In the absence of a gold standard criterion for diagnosing prosthetic joint infections (PJI), sonication of the removed implant may provide superior microbiological identification to synovial fluid and peri-implant tissue cultures. The aim of this retrospective study was to assess the role of sonication culture compared to tissue cultures for diagnosing PJI, using different consensus and international guidelines for PJI definition. Methods Data of 146 patients undergoing removal of hip or knee arthroplasties between 2010 and 2018 were retrospectively reviewed. The International Consensus Meeting (ICM-2018), Musculoskeletal Infection Society (MSIS), Infectious Diseases Society of America (IDSA), the European Bone and Joint Infection Society (EBJIS), and a modified clinical criterion, were used to compare the performance of microbiological tests. McNemar´s test and proportion comparison were employed to calculate p-value. Results Overall, 56% (82/146) were diagnosed with PJI using the clinical criteria. Out of these cases, 57% (47/82) tested positive on tissue culture and 93% (76/82) on sonication culture. Applying this clinical criterion, the sensitivity of sonication fluid and tissue cultures was 92.7% (95% CI: 87.1%- 98.3%) and 57.3% (95% CI: 46.6%-68.0%) (p<0.001), respectively. When both methods were combined for diagnosis (sonication and tissue cultures) sensitivity reached 96.3% (95% CI: 91.5%-100%). Sonication culture and the combination of sonication with tissue cultures, showed higher sensitivity rates than tissue cultures alone for all diagnostic criteria (ICM-18, MSIS, IDSA and EBJIS) applied. Conversely, tissue culture provided greater specificity than sonication culture for all the criteria assessed, except for the EBJIS criteria, in which sonication and tissue cultures specificity was 100% and 95.3% (95% CI: 87.8–100%), respectively (p = 0.024). Conclusions In a context where diagnostic criteria available have shortcomings and tissue cultures remain the gold standard, sonication cultures can aid PJI diagnosis, especially when diagnostic criteria are inconclusive due to some important missing data (joint puncture, histology).

School Districts Stumbled on Data Privacy

2012 ◽  
pp. 12-15 ◽  
Author(s):  
Irene Chen
Keyword(s):  
Public Schools ◽  
School Districts ◽  
Middle Schools ◽  
Social Security ◽  
Private Information ◽  
Web Site ◽  
Data Privacy ◽  
Personal Information ◽  
Personal Data ◽  
Short Period

The story describes how three school institutes are grappling with the loss of private information, each through a unique set of circumstances. Pasadena City Public Schools discovered that it had sold several computers containing the names and Social Security numbers of employees as surplus. Stephens Public Schools learned that personal information about students at one of its middle schools was lost when a bag containing a thumb drive was stolen. Also, Woodlands Public Schools accidentally exposed employee personal data on a public Web site for a short period of time. How should each of the institutes react?

Studies on the Biosynthesis of Pseudoisoeugenols in Tissue Cultures of Pimpinella anisum

1988 ◽  
Vol 43 (5-6) ◽  
pp. 328-336 ◽  
Author(s):  
Rainer Martin ◽  
Gerhard Schilling ◽  
Jürgen Reichling
Keyword(s):  
Tissue Culture ◽  
Cinnamic Acid ◽  
Tissue Cultures ◽  
Coumaric Acid ◽  
Pimpinella Anisum

A leaf-differentiating tissue culture which produced substantial amounts of pseudoisoeugenol- (2-methylbutyrate) has been used to examine the origin of the pseudoisoeugenol skeleton. 14Cand 13C-labelling revealed ʟ-phenylalanine, trans-cinnamic acid and p-coumaric acid as precursors. 13C-labelled precursors proved to be especially useful.

scholarly journals 1423. Antibiotic Utilization and Outcomes in Patients with Sacral Osteomyelitis and Decubitus Ulcers

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S518-S519
Author(s):  
Kelly Carpenter Byrge ◽  
Amanda Bailey ◽  
Bryan Harris ◽  
Thomas Talbot ◽  
Patty Wright ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Bone Biopsy ◽  
Antibiotic Use ◽  
Decubitus Ulcer ◽  
Advanced Stage ◽  
Tissue Cultures ◽  
Decubitus Ulcers ◽  
Clinical Concern ◽  
Antibiotic Utilization ◽  
Sacral Osteomyelitis

Abstract Background Osteomyelitis is a common complication of advanced stage pressure ulcers and known to be associated with increased mortality, length of stay (LOS), and treatment costs. However, limited data and lack of guidelines regarding appropriate diagnosis and treatment result in variability in management. We sought to investigate whether microbiology data are associated with reduced antibiotic utilization in patients with osteomyelitis secondary to decubitus ulcers. Methods This retrospective cohort study included hospitalized patients from 2007 to 2015 with an advanced stage (IV or unstageable) sacral decubitus ulcer and clinical concern for osteomyelitis. The exposure group was those who underwent tissue culture (superficial swab, deep bedside or surgical tissue, or bone biopsy). The primary outcome was antibiotic days of therapy (DOT). Additional secondary outcomes including clinical characteristics at presentation, LOS, readmission rates, and antibiotic-related complications were evaluated using Fisher’s exact or Wilcoxon-Mann–Whitney test for continuous variables. Results A total of 220 cases of advanced-stage decubitus ulcer with clinical concern for osteomyelitis were identified. Data abstracted from 40 cases show that tissue cultures were obtained in 22 (55%). Bacterial growth was identified from 100% of samples sent for culture. Antibiotic use prior to admission was the most significant predictor of failure to obtain tissue cultures (P = 0.0002). MRI was performed in 15% of abstracted cases, with radiographic evidence of osteomyelitis noted in 100%. Bone biopsy was performed in 4 cases; bone pathology was not sent in any of these instances. Median antibiotic DOT was 84 days in both groups. Conclusion In cases of sacral osteomyelitis secondary to decubitus ulcers, antibiotic use prior to admission was inversely related to the likelihood of obtaining a tissue culture. When tissue cultures were obtained, they were uniformly positive; however, in our preliminary analysis of 40 cases, this did not appear to influence antibiotic utilization as determined by DOT. Bone biopsy was rarely performed, and when done, was not sent for pathology despite this being recognized as the gold standard in the diagnosis of osteomyelitis. Disclosures All authors: No reported disclosures.

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