550 Mapping of QTL for Partial Physiological Resistance and Field Reaction to White Mold, Plant Architecture, and Plant Height in Common Bean

The objective was to detect molecular markers associated with QTL for partial physiological resistance (PPR) to two white mold (WM) isolates, partial field resistance (PFR), plant architecture (PA), and plant height (PH) in a genetic linkage map constructed using recombinant inbred lines (RILs) from the cross `PC-50' (resistant to WM) × XAN-159 (susceptible to WM). Significant correlations (+0.39 and +0.47) were noted between the WM reactions in the greenhouse and field. A significant but negative correlation (–0.33) was observed between the WM reaction and PH in the field. Six QTL affecting PPR to isolate 152 were found on LGs 4, 5, 7, and 8. Six QTL affecting PPR to isolate 279 were found on LGs 2, 3, 4, 7, and 8. Five QTL for PFR were observed on LGs 2, 5, 7, 8, and 11. Two QTL affecting PA were detected on LGs 7 and 8. Two QTL affecting PH were identified on LGs 7 and 8. On one end of LG 8 marker H19.1250 was significant for PPR to both isolates. On the other end of LG 8 the region closely linked to the C locus was significantly associated with PPR to both isolates, PFR, PA and PH. Marker J09.950 on LG 7 was significantly associated with PPR to both isolates, PFR, PH and seed weight. Marker J01.2000 on LG 2 was the most significant locus for both PPR to the isolate 279 and PFR. QTL on LG 5 were found for PPR to the isolate 152 and PFR. Overall, four of the five QTL affecting PFR were also found for PPR to one or both isolates.

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A high-resolution genetic linkage map of soybean based on 357 recombinant inbred lines genotyped with BARCSoySNP6K

Molecular Breeding ◽

10.1007/s11032-015-0209-5 ◽

2015 ◽

Vol 35 (2) ◽

Cited By ~ 16

Author(s):

Sungwoo Lee ◽

Keith R. Freewalt ◽

Leah K. McHale ◽

Qijian Song ◽

Tae-Hwan Jun ◽

...

Keyword(s):

High Resolution ◽

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Recombinant Inbred Lines ◽

Inbred Lines

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Screening for Partial Physiological Resistance to White Mold in Dry Bean Using Excised Stems

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.117.2.321 ◽

1992 ◽

Vol 117 (2) ◽

pp. 321-327 ◽

Cited By ~ 16

Author(s):

P.N. Miklas ◽

K.F. Grafton ◽

B.D. Nelson

Keyword(s):

Phaseolus Vulgaris ◽

Sclerotinia Sclerotiorum ◽

Field Resistance ◽

White Mold ◽

Lesion Length ◽

Laboratory Procedure ◽

Dry Bean ◽

Snap Bean ◽

Breeding Lines ◽

Physiological Resistance

A laboratory procedure was tested to determine whether excised stems would allow a reliable indication of partial physiological resistance (PPR) to white mold [Sclerotinia sclerotiorum (Lib.) deBary] in dry bean (Phaseolus vulgaris L.). Excised stems from 11- and 28-day-old plants were inoculated with growing mycelium of S. sclerotiorum, incubated for 4 to 7 days (11- and 28-day assays, respectively), then assayed for lesion length (LL). A total of 15 bean genotypes were screened for PPR, as indicated by LL. Significant (P < 0.05) differences among LL means of small- and medium-seeded bean genotypes were detected in the 28-day assay, whereas only LL means among medium-seeded genotypes. differed significantly (P < 0.05) in the n-day assay. `Bunsi', `C-20', `Sierra', `Topaz', and snap bean breeding lines NY 5262, NY 5394, and NY 5403 had the highest PPR and `Upland', D76125, and `UI-114' the lowest. The results from both assays were repeatable. A moderately high correlation (r = 0.68, P < 0.02) was observed between PPR and field resistance. The 28-day assay has potential for evaluating dry bean germplasm for PPR to white mold disease caused by S. sclerotiorum. A 28-day assay also was used to measure virulence of 18 isolates of S. sclerotiorum. The 18 isolates did not differ (P < 0.05) for virulence when measured by LL. The lack of any genotype × isolate interaction for LL indicated lack of host-pathogen specificity.

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Genetic resolution and verification of quantitative trait loci for flowering and plant height with recombinant inbred lines of maize

Genome ◽

10.1139/g96-120 ◽

1996 ◽

Vol 39 (5) ◽

pp. 957-968 ◽

Cited By ~ 43

Author(s):

David F. Austin ◽

Michael Lee

Keyword(s):

Quantitative Trait Loci ◽

Plant Height ◽

Quantitative Trait ◽

Recombinant Inbred ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Length Polymorphisms ◽

Trait Loci ◽

Ear Height ◽

Single Factor Analysis

Recombinant inbred (RI) lines offer several advantages for detecting quantitative trait loci (QTLs), including increased precision of trait measurements, power for detection of additive effects, and resolution of linked QTLs. This study was conducted to detect and characterize QTLs in maize for flowering and plant height and to compare QTL detection in an early (F2:3) generation of the same population. One hundred and eighty-six RIs from a cross between inbred lines Mo17 and H99 were evaluated in a replicated field experiment and analyzed at 101 loci detected by restriction fragment length polymorphisms. QTLs were identified by single-factor analysis of variance. A total of 59 QTLs were detected for plant height, ear height, top height, anthesis, silk emergence, and anthesis to silk interval. Individual QTLs explained 2.2–15.4% of trait variation, and multiple models including all QTLs detected for a trait explained up to 52.5% of the phenotypic variation. Comparison of QTLs detected with 150 F2:3 lines from the same population indicated that 16 (70%) of the 23 F2:3 QTLs were also observed in the F6:7 generation. Parental effects were consistent across generations. At 14 of the 16 QTLs detected in both generations, genetic effects were smaller in the F6:7. Also, some QTLs detected in the F2:3 were resolved into multiple linked QTLs in the F6:7, indicating the additional power of RI populations for mapping, with important implications for marker-assisted selection as well as map-based cloning of QTLs. Key words : Zea mays, RFLP, plant breeding, genetics, recombination.

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A genetic linkage map for Tribolium confusum based on random amplified polymorphic DNAs and recombinant inbred lines

Insect Molecular Biology ◽

10.1046/j.1365-2583.2003.00438.x ◽

2003 ◽

Vol 12 (5) ◽

pp. 517-526 ◽

Cited By ~ 5

Author(s):

A. Yezerski ◽

L. Stevens ◽

J. Ametrano

Keyword(s):

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Tribolium Confusum

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Genetic dissection of plant height by molecular markers using a population of recombinant inbred lines in maize

Euphytica ◽

10.1007/s10681-006-9312-3 ◽

2006 ◽

Vol 155 (1-2) ◽

pp. 117-124 ◽

Cited By ~ 47

Author(s):

Tang Ji-hua ◽

Teng Wen-tao ◽

Yan Jian-bing ◽

Ma Xi-qing ◽

Meng Yi-jiang ◽

...

Keyword(s):

Molecular Markers ◽

Plant Height ◽

Recombinant Inbred ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Genetic Dissection

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Genetic analysis and QTL mapping of growth period traits and plant height traits in soybean recombinant inbred lines from Dongnong 47 × PI 317334-B

Oil Crop Science ◽

10.1016/j.ocsci.2021.04.001 ◽

2021 ◽

Author(s):

Jing Li ◽

Jiafan Sun ◽

Minmin Li ◽

Xue Zhao ◽

Lin Zhao

Keyword(s):

Genetic Analysis ◽

Qtl Mapping ◽

Plant Height ◽

Recombinant Inbred ◽

Recombinant Inbred Lines ◽

Growth Period ◽

Inbred Lines

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Quantitative Trait Loci Mapping of Stem oBrix Content and Stem Diameter in Sorghum Recombinant Inbred Lines Using Genotype-By-Sequencing

10.21203/rs.3.rs-721686/v1 ◽

2021 ◽

Author(s):

Abera Takele Deressa ◽

Tileye Feyisa ◽

Tesfaye Disasa

Keyword(s):

Sweet Sorghum ◽

Significant Contribution ◽

Genetic Linkage Map ◽

Recombinant Inbred Lines ◽

Sugar Content ◽

Growing Season ◽

Inbred Lines ◽

Snp Markers ◽

Stem Diameter ◽

Genotype By Sequencing

Abstract Sweet sorghum has the ability to store sugar in its stem. Many sugar content genes have been discovered and through breeding worldwide. However, some of these genes are unstable. This study aimed to detect and validate QTL for stem oBrix content from sorghum RILs. In two seasons, QTL linked with stem brix content and related traits were examined using 139 F8RILs from a grain and sweet sorghum. A genetic linkage map with 128 SNP markers was created and several QTLs were identified. Phenotypic variation between 6.33 and 14% was identified for a given trait. Over two seasons, four QTLs for stem brix content (qBrix2-1, qBrix4-1, qBrix4-2, and qBrix10-1) and three QTLs for stem diameter (qSD1-1, qSD8-1 and qSD9-1) were detected. The detected QTL could be useful for improving stem brix content in different growing season. Furthermore, it makes a significant contribution to marker-assisted brix selection and sorghum biofuel improvement.

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Identification of QTLs for capsaicinoids, fruit quality, and plant architecture-related traits in an interspecific Capsicum RIL population

Genome ◽

10.1139/gen-2012-0083 ◽

2013 ◽

Vol 56 (1) ◽

pp. 61-74 ◽

Cited By ~ 32

Author(s):

Shawn C. Yarnes ◽

Hamid Ashrafi ◽

Sebastian Reyes-Chin-Wo ◽

Theresa A. Hill ◽

Kevin M. Stoffel ◽

...

Keyword(s):

Fruit Quality ◽

Plant Architecture ◽

Mapping Population ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Quality Traits ◽

Capsicum Frutescens ◽

Fruit Quality Traits ◽

Ril Population ◽

Tight Linkage

Quantitative trait loci (QTL) analyses in pepper are common for horticultural, disease resistance, and fruit quality traits; although none of the studies to date have used sequence-based markers associated with genes. In this study we measured plant architectural, phenological, and fruit quality traits in a pepper mapping population consisting of 92 recombinant inbred lines derived from a cross between Capsicum frutescens acc. 2814-6 and C. annuum var. NuMexRNAKY. Phenotypic measurements were correlated to loci in a high-density EST-based genetic map. In total, 96 QTL were identified for 38 traits, including 12 QTL associated with capsaicinoid levels. Twenty-one loci showed correlation among seemingly unrelated phenotypic categories, highlighting tight linkage or shared genetics between previously unassociated traits in pepper.

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Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker

Genetika ◽

10.2298/gensr1502425c ◽

2015 ◽

Vol 47 (2) ◽

pp. 425-438 ◽

Cited By ~ 2

Author(s):

Li-Jing Chen ◽

Hong-Mei Li ◽

Shao-Kun Sun ◽

Muhammad Irfan ◽

Jing-Wei Lin ◽

...

Keyword(s):

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Mapping Population ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Linkage Groups ◽

Marker Interval ◽

Interval Distance

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.

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