scholarly journals Transgenic Resistance to the Colorado Potato Beetle in Bt-expressing Eggplant Fields

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 722-725 ◽  
Author(s):  
Nazareno Acciarri ◽  
Gabriele Vitelli ◽  
Salvatore Arpaia ◽  
Giuseppe Mennella ◽  
Francesco Sunseri ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
Transgenic Line ◽  
Block Design ◽  
Total Yield ◽  
Solanum Melongena ◽  
Fruit Production ◽  
Lower Amount ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Beetle ◽  
Transgenic Lines

Colorado potato beetle (CPB; Leptinotarsa decemlineata-Say) is a serious pest because it has developed resistance against insecticides. Three transgenic eggplant (Solanum melongena L.) lines bearing a mutagenized Bacillus thuringiensis Berl. gene coding for the Cry3B toxin, and the nontransformed control DR2-line were tested in field trials to assess their insect resistance. The transgenic lines 3-2, 6-1, and 9-8 were tested at two different locations in a randomized complete-block design. Samples were taken biweekly to assess the level of CPB and the presence of other insects. At harvest, total yield and fruit number per plot were recorded. Two transgenic lines showed high levels of resistance at both locations, as measured by CPB abundance and yield. Fruit production was almost twice as great in the highly resistant lines (3-2 and 9-8) as in the nontransformed control. The 6-1 transgenic line showed an intermediate level of resistance; it was similar to the control under heavy CPB pressure and was comparable to the other transgenic lines under milder infestations. Analysis by double antibody sandwich–enzyme linked immunosorbent assay (DAS–ELISA), performed on different tissues, revealed a lower amount of Cry3B protein in the 6-1 transgenic line than in lines 3-2 and 9-8. No detrimental effects on nontarget arthropods (including the chrysomelid Altica) were evident. Field observations confirmed that Bt may be able to control CPB infestation in eggplant, representing a potential effective and environmentally safe means of pest control.

scholarly journals Accumulation of degree days and their effect on the potential yield of 15 eggplant (Solanum melongena L.) accessions in the Colombian Caribbean

2019 ◽  
Vol 72 (3) ◽  
pp. 8917-8926
Author(s):  
Rommel Igor León Pacheco ◽  
Ender Manuel Correa Álvarez ◽  
Jorge Luis Romero Ferrer ◽  
Heriberto Arias Bonilla ◽  
Juan Camilo Gómez-Correa ◽  
...  
Keyword(s):  
Block Design ◽  
Total Yield ◽  
Solanum Melongena ◽  
Principal Component ◽  
Fruit Production ◽  
Caribbean Region ◽  
Degree Days ◽  
Potential Yield ◽  
The Caribbean ◽  
Time Required

The growing degree-days (GDD) provide an estimate of the accumulated thermal energy available for the development of a crop. The use of GDD allows measuring the heat requirements associated with the phenological stages of the crop, which allows in turn, to predict when a certain plant stage will occur knowing the daily temperatures. The aim of this study was to establish relationships among the effect of degree days (DD) to vegetative growth, first flowering and fructification (VG, Fl and Fr), on total yield per plant (TY/P) of eggplant grown under open-field conditions employing a randomized complete block design with 15 genotypes and four replicates. The results showed that: 1) The genotypes that initiated fruit production in less time required fewer GDD (892.14-1,077.71 °C) for this phenological phase, obtaining higher productivity. 2) The genotypes C035 and C040 had an average yield higher than the national average with values of 83.75 and 84.86 t ha-1, being identified as future varieties to be produced in the Caribbean region. 3) The Caribbean region is suitable for the establishment of the crop as there were no events with limiting temperatures for this species (higher than 35 °C and lower than 15 °C). 4) The principal component analysis showed associations among the variable YT/P with the genotypes C011, C042, and C015; meanwhile, C032, C025, and C028 were associated with the variables DD to VG, Fl, and Fr. These results would be useful in developing a model to estimate yield with DD.

scholarly journals Expression of heterosis for productive traits in F1 eggplant (Solanum melongena L.) hybrids

1998 ◽  
Vol 21 (1) ◽  
pp. 99-103
Author(s):  
João Alencar de Sousa ◽  
Wilson Roberto Maluf
Keyword(s):  
Block Design ◽  
Total Yield ◽  
Solanum Melongena ◽  
Fruit Production ◽  
Fruit Weight ◽  
Fruit Yield ◽  
F1 Hybrids ◽  
Breeding Lines ◽  
Early Production ◽  
Productive Traits

This study was carried out to obtain estimates of heterosis in crosses between seven eggplant cultivars (Embu = E; Santa Genebra = SG; Viserba = V; Aubergine de Barbentane = AB; Florida Market 10 = FM; Black Beauty = BB, and Melitino = M) and two breeding lines (B-14-07 = B1 and B-31-06 = B2). The F1 hybrids used were: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 and M x FM. Cultivars, lines and hybrids were evaluated at the ESAL experimental field in Lavras, MG, from February to October 1992. A randomized complete block design with three replications was used. Significant heterosis relative to the parental means was detected for all traits studied. Their values ranged from +41.23% to +113.31% for total fruit yield, from -11.45% to +26.17% for average fruit weight, and from +27.98% to +141.81% for early production. Heterosis relative to the superior parent ranged from +13.89% to +92.51% for total fruit yield. Hybrid pairs: SG x FM and AB x B1, V x FM and AB x FM, E x M and AB x B1 were the most heterotic relative to the parental mean for total fruit production, mean fruit weight and early production, respectively. The hybrids displaying highest heterosis relative to the superior parent for total yield were AB x B1 and SG x FM.

scholarly journals COLORADO POTATO BEETLE CONTROL WITH CYROMAZINE

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 628e-628 ◽  
Author(s):  
J.D. Abbott ◽  
L. T. Thetford
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Total Yield ◽  
Insect Growth Regulator ◽  
Insect Feeding ◽  
Potato Leaf ◽  
Egg Hatch ◽  
Potato Beetle ◽  
Untreated Check ◽  
Small Plot

Cyromazine is a triazine molecule with insect growth regulator properties being developed for control of Colorado potato beetle (Leptinotarsa decemlineata Say) (CPB) in vegetables. Research presented focuses primarily on results with potato (Solanum tuberosum L.), however, crop safety has been observed in other crops within the Solanaceae. Several trials were conducted in PA and NY during 1991 to examine the rates and timing necessary to control CPB in potatoes. Data from replicated small plot trials and non-replicated large block trials are included. Rates examined ranged 70 to 560 g ai na-1 applied alone or in combination with a pyrethroid or Bt. Comparisons were made with insecticides presently registered for CPB control in potatoes and cyromazine compared quite favorably. Two applications per CPB generation were made, the first at the beginning of CPB egg hatch and a second 7-16 days later for each generation. This application schedule provided excellent (90%) control of CPB larvae. The reduction in larvae also resulted in a reduction in adult CPB and potato leaf area damaged through insect feeding. In the test conducted in PA, an increase in size and number of tubers was observed when plants were treated with cyromazine. -These increases resulted in a 23-28% increase in total yield compared to that obtained from the untreated check plots.

scholarly journals Field Evaluation of Natural, Engineered, and Combined Resistance Mechanisms in Potato for Control of Colorado Potato Beetle

2003 ◽  
Vol 128 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Joseph J. Coombs ◽  
David S Douches ◽  
Wenbin Li ◽  
Edward J. Grafius ◽  
Walter L. Pett
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Resistance Mechanisms ◽  
Potato Beetle ◽  
Transgenic Lines ◽  
Number Of Individuals

The colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance has the potential use in an integrated pest management program for control of colorado potato beetle. During the 1998 and 1999 seasons, field studies were conducted to compare natural (leptine glycoalkaloids and glandular trichomes), engineered (Bt-cry3A and Bt-cry5 transgenic potato lines), and combined (Bt-cry5+glandular trichomes) plant resistance mechanisms of potato for control of colorado potato beetle. Nine different potato clones representing five different host plant resistance mechanisms were evaluated under natural colorado potato beetle infestation at the Montcalm Research Farm in Entrican, Michigan. The Bt-cry3A transgenic lines, the high leptine line (USDA8380-1), and the high foliar glycoalkaloid line (ND5873-15) were most effective for controlling defoliation by colorado potato beetle adults and larvae. The Bt-cry5 line (SPc5-G2) was not as effective as the Bt-cry3A transgenic lines ('Russet Burbank Newleaf,' RBN15, and YGc3.1). The glandular trichome (NYL235-4) and Bt-cry5+glandular trichome lines proved to be ineffective. Significant rank correlations for the potato lines between the two years were observed for egg masses, second and third instar, and fourth instar seasonal cumulative mean number of individuals per plant, and defoliation. Egg mass and first instar seasonal cumulative mean number of individuals per plant were not strong indicators of host plant resistance in contrast to second and third instars or adults. Based on these results, the Bt-cry3A transgenic lines, the high leptine line, and the high total glycoalkaloid line are effective host plant resistance mechanisms for control of colorado potato beetle.

scholarly journals Combining Natural and Engineered Host Plant Resistance Mechanisms in Potato for Colorado Potato Beetle: Choice and No-choice Field Studies

2005 ◽  
Vol 130 (6) ◽  
pp. 857-864 ◽  
Author(s):  
Joseph J. Coombs ◽  
David S. Douches ◽  
Susannah G. Cooper ◽  
Edward J. Grafius ◽  
Walter L. Pett ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Glandular Trichomes ◽  
Field Studies ◽  
Resistance Mechanisms ◽  
Field Cage ◽  
Potato Beetle ◽  
Transgenic Lines

Colorado potato beetle (Leptinotarsa decemlineata Say) is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance is an important tool in an integrated pest management program for controlling insect pests. Field studies were conducted to compare natural host plant resistance mechanisms (glandular trichomes and Solanum chacoense Bitter-derived resistance), engineered [Bacillus thuringiensis (Bt) Berliner Bt-cry3A], and combined (glandular trichomes + Bt-cry3A and S. chacoense-derived resistance + Bt-cry3A transgenic potato lines) sources of resistance for control of colorado potato beetle. Six different potato clones representing five different host plant resistance mechanisms were evaluated for 2 years in a field situation under natural colorado potato beetle pressure in Michigan and New York, and in a no-choice field cage study in Michigan. In the field studies, the S. chacoense-derived resistance line, Bt-cry3A transgenic, and combined resistance lines were effective in controlling defoliation by colorado potato beetle adults and larvae. Effectively no feeding was observed in the Bt-cry3A transgenic lines. The glandular trichome line suffered less defoliation than the susceptible control, but had greater defoliation than the Bt-cry3A transgenic lines and the S. chacoense-derived resistance line. In the no-choice cage study, the Bt-cry3A transgenic lines and the combined resistance lines were effective in controlling feeding by colorado potato beetle adults and larvae with no defoliation observed. The S. chacoense-derived resistance line and the glandular trichome line suffered less defoliation than the susceptible control. Based on the results of the field trials and no-choice field cage studies, these host plant resistance mechanisms could be used to develop potato varieties for use in a resistance management program for control of colorado potato beetle.

scholarly journals Colorado Potato Beetle Control on Potatoes with In-Furrow Treatments, 1994

1995 ◽  
Vol 20 (1) ◽  
pp. 115-115
Author(s):  
James J. Linduska ◽  
Marylee Ross ◽  
Karen Mulford ◽  
John Nottingham
Keyword(s):  
Colorado Potato Beetle ◽  
Rating Scale ◽  
Block Design ◽  
Compressed Air ◽  
Single Treatment ◽  
Egg Masses ◽  
Row Crop ◽  
Potato Beetle ◽  
Randomized Complete Block Design ◽  
Direct Counts

Abstract ‘Kennebec’ potatoes were planted in 20 foot rows on 25 Apr, ‘94. Plots consisted of a single treatment row. The distance between rows was 64 inches and the distance between plants within the row was 10 inches. Plots were arranged in a randomized complete block design with 4 replications. The soil was a Norfolk "A" loamy sand. Initial furrow treatments were applied to the seed at the rate of 20 gal per acre with a small watering can. Admire foliar treatments were applied at a rate of 50 gal per acre on 8, 23 and 29 Jun with a custom built compressed air row crop sprayer with each row being covered by 6 drop nozzles delivering 50 gal/acre at 30 psi. In evaluating the treatments, direct counts of the number of Colorado potato beetle adults, larvae and egg masses/10 plants and the % defoliation were recorded. Defoliation categories were assigned using a pretransformed defoliation rating scale, based on dividing 90° into 13 steps corresponding to arcsin transformation values (Little & Hills 1978). Yields were taken on 20 Jul from 20-ft of row.

scholarly journals Colorado Potato Beetle Control, 1993

1994 ◽  
Vol 19 (1) ◽  
pp. 110-110
Author(s):  
Casey W. Hoy ◽  
Michael J. Dunlap
Keyword(s):  
Seed Piece ◽  
Colorado Potato Beetle ◽  
First Generation ◽  
Block Design ◽  
Plant Spacing ◽  
Single Application ◽  
Hollow Cone ◽  
Potato Beetle ◽  
Randomized Complete Block Design ◽  
Vine Killer

Abstract ‘Gemchip’ potatoes were planted 26 May at the Fry Farm, OARDC, Wooster, OH. Plant spacing was 36 inches between rows and 9 inches between plants. Three Admire 2F seed piece treatments were applied at planting using a CO2 compression sprayer at 40 psi with an 8004 flat fan nozzle delivering the insecticide into the seed furrow. The field was treated on 16 Jun with 0.75 lb/ acre of Sencor DF. Fungicide, Dithane at 2.0 lb/acre, was applied 9 Aug. Paraquat, 1 pt/acre, was applied 7 Sep as a vine killer. Plots were two rows wide and 40 ft in length, with 10 ft alleys separating plots and 6 ft alleys separating blocks. Treatments were arranged in a randomized complete block design. Foliar treatments were initiated when the first generation larvae were increasing in the plots in early Jul. Foliar applications were made 3 and 12 Jul and 3 and 12 Aug. The Trigard single application treatment was applied only on 3 Jul and 3 Aug. Foliar treatments were applied with a tractor mounted drop nozzle boom sprayer delivering 50 gal/acre at 50 psi with three D-4 hollow cone nozzles/row. Bond spreader sticker was added at 4 oz/ 100 gal solution to all rates of the following insecticides: Prokill Cryolite 96, AC303,630, M-Trak, and Novodor. Number of eggs, small larvae (instar 1-2), large larvae (instar 3-4), and adults were counted, and percent defoliation was visually estimated, in five 1-m lengths of row per plot approximately five days after each application.

scholarly journals Control of Resistant Colorado Potato Beetle in Early Market Potato, Big Lake, Mn, 1993

1994 ◽  
Vol 19 (1) ◽  
pp. 115-115
Author(s):  
David Noetzle ◽  
Jennifer Miller
Keyword(s):  
Colorado Potato Beetle ◽  
Block Design ◽  
Potato Field ◽  
Synthetic Materials ◽  
Potato Beetle ◽  
Commercial Potato ◽  
Row Width ◽  
Randomized Complete Block Design ◽  
The Individual ◽  
Early Market

Abstract Continued failure of most insecticides for control of CPB in the Anoka Sand Plains growing area stimulated further comparisons of biologicals, inorganics, and synthetic materials for CPB management. Soils are sands at this location. Plots were established in an irrigated commercial potato field. The individual plot consisted of 4—25 ft rows with a row width of 3 ft. Treatments were arranged in a randomized complete block design and were replicated 4 times. The grower treated all of the field, including the plots, with Thiodan and Asana XL at 1.0 lb and 0.5 lb Al/acre respectively on 8 Jul and 16 Jul. Plot treatments were applied on 25 Jun, 6 and 19 Jul using a CO2 hand sprayer with about 20 gals total material/acre and 40 psi pressure. Larval ratings and defoliation estimates were taken on 6 and 19 Jul. Vines were killed on 3 Aug and yields collected on 18 Aug. The center 2 rows of each plot were harvested for yield.

scholarly journals Production of paulista gherkin using trellis net support

2004 ◽  
Vol 61 (1) ◽  
pp. 43-46
Author(s):  
Valéria A. Modolo ◽  
Cyro Paulino da Costa
Keyword(s):  
Fruit Quality ◽  
Block Design ◽  
Total Yield ◽  
Fruit Production ◽  
Fruit Weight ◽  
Yield And Quality ◽  
Randomized Block Design ◽  
Plot Yield ◽  
Length Width ◽  
Single Cross Hybrids

Paulista gherkin is a new gherkin type obtained by crossing Cucumis anguria var. anguria x C. anguria var. longaculeatus. It differs from common gherkin in its fruits and leaves. Elite lines of Paulista gherkin present similar performance for total yield and fruit weight but some have distinctive characteristics, including fruit and leaf attributes. The combination of these characteristics through production of Paulista gherkin hybrids could be an alternative for fruit quality and/or yield improvement. The purpose of the present work was to compare the yield of Paulista gherkin lines and their hybrids grown on a trellis net under field conditions. Four lines and their six single-cross hybrids were evaluated for yield and fruit production using the trellised net production system. Seedlings were produced in polystyrene trays with 128 cells, and transplanted to the middle of 1.20 m wide beds. Plants were trained without pruning on netting having 0.1 x 0.1 m openings. The experiment was conducted in a randomized block design with four replicates and seven plants per plot. Yield was expressed as number of fruits and total weight of fruits per plot. Length, width and fruit flesh thickness were also evaluated, with five samples per plot in two harvesting times. Hybrids and their parental lines were similar in fruit yield and quality. The trellised net was suitable for Paulista gherkin production and provided adequate support to the plants. The trellis technique is suitable to make harvesting easier and to improve fruit quality.

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