scholarly journals Prevalence of Citrus Tristeza Virus in Florida Citrus Nurseries and Scion Groves

HortScience ◽  
2003 ◽  
Vol 38 (2) ◽  
pp. 244-245 ◽  
Author(s):  
Charles A. Powell ◽  
Robert R. Pelosi ◽  
Phyllis A. Rundell
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nursery Trees ◽  
Florida Citrus ◽  
High Level ◽  
Individual Trees ◽  
Tristeza Virus ◽  
Composite Samples ◽  
Citrus Tristeza

None of 4190 sweet orange [Citrus sinensis (L.) Osb.] nursery trees of `Hamlin', `Midsweet', `Navel', and `Valencia' sampled from five Florida citrus nurseries were infected with a decline-inducing isolate of citrus tristeza virus (CTV) as judged by enzyme-linked immunosorbent assay (ELISA) using isolate-specific monoclonal antibodies. Two of the nurseries had a relatively high level of infection (37% to 100% of composite samples containing tissue from 10 trees) with nondecline-inducing (mild) isolates of CTV, depending on the cultivar. Three of the nurseries had a lower incidence of mild CTV (0% to 22% of 10 tree composite samples). No nursery was CTV-free. ELISA of individual trees used as budwood sources by the nurseries revealed that one tree out of 260 tested contained decline-inducing CTV, and 83 contained mild CTV. These results suggest that the budwood certification program adopted in 1997 has virtually eliminated decline-inducing CTV from commercial budwood supplies.

scholarly journals First Report of Citrus tristeza virus in Greece

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 329-329 ◽  
Author(s):  
D. Dimou ◽  
J. Drossopoulou ◽  
E. Moschos ◽  
C. Varveri ◽  
F. Bem
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Vein Clearing ◽  
Carrizo Citrange ◽  
Sweet Lime ◽  
Tristeza Virus ◽  
Propagation Material ◽  
Citrus Tristeza

Large-scale surveys of Citrus spp. for the presence of Citrus tristeza virus (CTV) by the Ministry of Agriculture in Greece began in 1995. Over 26,000 trees have been tested by enzyme-linked immunosorbent assay and immunoprinting (2). In summer 2000, the first CTV-infected sweet orange cv. Lane Late tree grafted on CTV-tolerant Carrizo citrange was found in Argolis County, Peloponnese. This tree belonged to a batch of CAC propagation material (20 trees) illegally introduced from Spain in 1994, which was subsequently traced and found to be infected (45%). A follow-up search of trees grafted with the above material was undertaken in the two concerned regions (Argolis and Chania-Crete), and more than 3,500 trees have been removed. Extensive surveys continue to identify and destroy new infections. Few cases (15 of 16,800) of natural transmission to cultivars other than cv. Lane Late have been found. All of these have been close to the initially infected trees in the Argolis area. Surveys in spring 2001 were extended to certified propagation material of Clemenpons mandarin on Carrizo citrange imported from Spain, and 7 of 1,038 plants were infected (0.64%). The virus was successfully graft-transmitted to sweet orange cv. Madame Vinous and sweet lime seedlings, where it was identified by immunoprinting and reverse transcription-polymerase chain reaction (1). Mild vein clearing symptoms appeared on both indicators. Vein clearing on sweet lime was also accompanied by leaf cupping. To our knowledge, this is the first report of CTV in Greece. References: (1) A. Sambade et al. J. Virol. Methods 87:25, 2000. (2) C. Vela et al. J. Gen. Virol. 67:91, 1986.

scholarly journals Serological Differentiation of the Citrus Tristeza Virus Isolates Causing Stem Pitting in Sweet Orange

Plant Disease ◽  
1998 ◽  
Vol 82 (11) ◽  
pp. 1276-1280 ◽  
Author(s):  
Olga V. Nikolaeva ◽  
Alexander V. Karasev ◽  
Stephen M. Garnsey ◽  
Richard F. Lee
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Format ◽  
Strong Positive Signal ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Polyclonal Serum ◽  
Important Disease ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) complex comprises a number of isolates or strains producing several economically important disease syndromes in commercial Citrus spp. The stem pitting syndrome is the most important, and causes substantial losses in many citrus-producing regions of the world. In an attempt to develop a serological tool to rapidly differentiate stem pitting isolates of CTV, we evaluated many combinations of trapping and detecting antibodies in an indirect double-antibody sandwich (I-DAS) enzyme-linked immunosorbent assay (ELISA). Two combinations of trapping and detecting antibodies were found suitable for differentiating stem pitting isolates in extracts of infected sweet orange plants. One used a polyclonal serum raised against bacterially expressed CTV coat protein (CP) for trapping and a conformational monoclonal antibody 3E10 for detection, and the other used two polyclonal antisera generated against bacterially expressed CTV CP. Seventy-six CTV isolates from 20 countries, including 35 that cause stem pitting in sweet orange plants, were analyzed in I-DAS-ELISA using different combinations of polyclonal and monoclonal antibodies for trapping and as intermediate detecting antibodies. The ELISA format developed produces a strong positive signal for CTV isolates that cause stem pitting in sweet orange plants and a negative ELISA signal for CTV isolates that do not cause stem pitting. When combined with data on a universal ELISA format, i.e., reacting with a broad range of CTV isolates, these selective ELISA formats allowed reliable serological differentiation of CTV isolates that caused stem pitting in infected sweet orange plants.

scholarly journals Detection and Isolate Differentiation of Citrus tristeza virus in Infected Field Trees Based on Reverse Transcription-Polymerase Chain Reaction

Plant Disease ◽  
2004 ◽  
Vol 88 (6) ◽  
pp. 625-629 ◽  
Author(s):  
Zhipeng Huang ◽  
Phyllis A. Rundell ◽  
Xiong Guan ◽  
Charles A. Powell
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tristeza Virus ◽  
Citrus Tristeza

Reverse transcription-polymerase chain reaction (RT-PCR) was compared with enzyme-linked immunosorbent assay (ELISA) and direct tissue blot immunoassay (DTBIA) for detection of non-decline-inducing and decline-inducing isolates of Citrus tristeza virus (CTV) in 21 field sweet orange and grapefruit plants on sour orange rootstock in Fort Pierce, FL. Among these samples, seven, six, and eight were infected with decline-inducing, non-decline-inducing, and both decline-inducing and non-decline-inducing isolates of CTV, respectively. However, there was not a good correlation between field symptoms and detection of the decline-inducing isolate. The results confirmed that RT-PCR is not only able to detect and differentiate decline-inducing and non-decline-inducing isolates of CTV in Florida, but also can detect both isolate types in a single field sweet orange or grapefruit tree. For most samples, results from RT-PCR, ELISA, and DTBIA were the same. However, the 320-bp fragments produced only from decline-inducing isolates were amplified from two sweet orange and two grapefruit samples that did not react with decline-inducing CTV-specific monoclonal antibody MCA13 in ELISA or DTBIA, indicating that RT-PCR has a higher sensitivity than these immunological tests for field sweet orange or grapefruit samples. Thus, RT-PCR is a simple, rapid, and specific procedure for CTV identification applicable to both research and diagnostic needs.

scholarly journals Suppression of Decline-inducing Isolates of Citrus Tristeza Virus by Nondecline-inducing Isolates

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 62-64 ◽  
Author(s):  
Charles A. Powell ◽  
Phyllis A. Rundell ◽  
Robert R. Pelosi
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Minor Component ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sour Orange ◽  
Citrus Paradisi ◽  
Indicator Plants ◽  
Tristeza Virus ◽  
Swingle Citrumelo ◽  
Citrus Tristeza

Bark chips from six container-grown citrus trees, infected with nondecline-inducing citrus tristeza virus (CTV) isolates and maintained in a vector-free greenhouse for 10 years, 15 commercial grapefruit (Citrus paradisi Macf.) trees, and 16 commercial sweet orange [C. sinensis (L.) Osbeck] trees were used to inoculate three indicator plants each of `Madam Vinous' sweet orange [C. sinensis (L.) Osbeck], sour orange (C. aurantium L.), `Duncan' grapefruit (C. paradisi Macf.), `Mexican' lime [C. aurantifolia (Christm.)], Swingle citrumelo [C. paradisi Macf. × Poncirus trifoliota (L.) Raf.], and sour orange grafted with `Hamlin' sweet orange [C. sinensis (L.) Osbeck]. All plants providing bark chips had repeatedly tested positive by enzyme-linked immunosorbent assay (ELISA) for CTV [reacted with monoclonal antibody (MAb) 17G11], but tested negative for Florida decline-inducing isolates of CTV (did not react with MAb MCA13). After 6 months in vector-free greenhouses, all in oculated trees (except Swingle citrumelo, which is considered CTV resistant) were positive for CTV by 17G11 ELISA. In addition, some indicator plants inoculated from nine (two container, two commercial grapefruit, and five commercial orange trees) of the 37 bark chip source trees also were positive for decline-inducing CTV by MCA13 ELISA. Some of these positive indicators also showed vein-clearing symptoms characteristic of infection with a severe isolate of CTV. No control, noninoculated indicators in the same greenhouse, became infected with either decline-inducing or nondecline-inducing CTV. These results indicate that decline-inducing isolates of CTV can be present as a minor component of a mixture at levels undetectable by ELISA, and that these decline-inducing isolates can become detectable by ELISA and sometimes by symptoms when inoculated into indicator plants.

scholarly journals First Report from Syria of Citrus tristeza virus in Citrus spp.

Plant Disease ◽  
2008 ◽  
Vol 92 (10) ◽  
pp. 1468-1468
Author(s):  
R. Abou Kubaa ◽  
K. Djelouah ◽  
A. M. D'Onghia ◽  
R. Addante ◽  
M. Jamal
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Citrus Limon ◽  
Potential Threat ◽  
First Report ◽  
Mexican Lime ◽  
Vein Clearing ◽  
Citrus Varieties ◽  
Tristeza Virus ◽  
Citrus Tristeza

During the spring of 2006, the main Syrian citrus-growing areas of Lattakia (Jableh, Aledyye, Eseelya, Siano, and Hresoon provinces) and Tartous (Almintar, Aljammase, Karto, Majdaloonelbahr, Yahmour, Amreet, Althawra, and Safita provinces) were surveyed to assess the presence of Citrus tristeza virus (CTV). Eight nurseries (approximately 130 plants per nursery), two budwood source fields (approximately 230 trees per field), and 19 groves (approximately 60 trees per grove) containing the main citrus varieties were visually inspected and sampled for serological assays. The hierarchical sampling method was carried out in each selected grove (2). Infected samples were collected from two nurseries, two budwood source fields, and six groves. Stems and leaf petioles from nursery trees and flower explants from the groves were collected and analyzed for CTV by direct tissue blot immunoassay (DTBIA) with the commercial kit from Plantprint (Valencia, Spain). Of 2,653 samples tested, 89 (4%) CTV-infected plants were detected. Five citrus varieties were found to be infected and Meyer lemon (Citrus limon ‘Meyer’) had the highest incidence at 16%. Numerous sweet orange varieties (Citrus sinensis L.) were found to be highly infected in the field, but only the Washington navel sweet orange was found to be infected in the nurseries. No clear CTV symptoms were observed during the survey. Samples that were positive for CTV by DTBIA were also positive by biological indexing on Mexican lime (C. aurantifolia) and immunocapture-reverse transcription-PCR as described by Nolasco et al. (3). Coat protein gene sequences obtained from five selected clones of a Syrian CTV isolate (GenBank Accession No. EU626555) showed more than 99 and 98% nucleotide sequence identity to a Jordanian CTV isolate (GenBank Accession No. AY550252) and the VT isolate (GenBank Accession No. U56902), respectively. Almost all infected samples induced moderate vein clearing symptoms when grafted to Mexican lime. Symptoms of vein clearing, leaf cupping, stunting, and stem pitting on Mexican lime were induced by graft transmission of CTV from one Valencia sample from the Tartous area. The viral inoculum is widely and randomly distributed in commercial groves, especially in the southern Tartous area and in some nurseries. To our knowledge, this is the first report of CTV in Syria. However, CTV was reported from the neighboring citrus-growing countries of Lebanon, Turkey, and Jordan (1), and the severe seedling yellows strain is present in this area, which poses a potential threat to Syrian citriculture. References: (1) G. H. Anfoka et al. Phytopathol. Mediterr. 44:17, 2005. (2) G. Hughes and T. R. Gottwald, Phytopathology 88:715, 1998. (3) G. Nolasco et al. Eur. J. Plant Pathol. 108:293, 2002.

scholarly journals Agronomic performance of Pera and related sweet orange accessions naturally infected with Citrus tristeza virus in northern Paraná State, Brazil

2014 ◽  
Vol 39 (6) ◽  
pp. 442-448 ◽  
Author(s):  
Viviane Y. Baba ◽  
José S. Giampani ◽  
Zuleide H. Tazima ◽  
Inês F. U. Yada ◽  
Luzia D. Paccola-Meirelles ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Agronomic Performance ◽  
Tristeza Virus ◽  
Citrus Tristeza

Exotic Citrus Diseases: Early Detection is the Solution to Protecting Florida Citrus

EDIS ◽  
2009 ◽  
Vol 2009 (3) ◽  
Author(s):  
Jamie D. Burrow (Yates) ◽  
Megan M. Dewdney ◽  
Ronald H. Brlansky
Keyword(s):  
Early Detection ◽  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Contact Information ◽  
Citrus Diseases ◽  
Citrus Leprosis ◽  
Florida Citrus ◽  
Pdf Format ◽  
Tristeza Virus ◽  
Stem Pitting

CH202, a 2-page illustrated trifold brochure by J.D. Burrow, M.M. Dewdney, and R.H. Brlansky, is best viewed in PDF format. It provides key information about citrus blackspot, sweet orange scab, citrus leprosis virus, citrus variegated chlorosis (CVC), and citrus tristeza virus (CTV) stem pitting. Includes contact information for UF/IFAS Extension citrus experts. Published by the UF Department of Plant Pathology, November 2008. CH202/CH202: Exotic Citrus Diseases: Early Detection is the Solution to Protecting Florida Citrus (ufl.edu)

scholarly journals Breakdown of Cross-Protection of Grapefruit from Decline-Inducing Isolates of Citrus tristeza virus Following Introduction of the Brown Citrus Aphid

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1116-1118 ◽  
Author(s):  
C. A. Powell ◽  
R. R. Pelosi ◽  
P. A. Rundell ◽  
M. Cohen
Keyword(s):  
Immunosorbent Assay ◽  
Citrus Tristeza Virus ◽  
Cross Protection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Experimental Area ◽  
Toxoptera Citricida ◽  
The Cross ◽  
Tristeza Virus ◽  
Citrus Tristeza

A 21-year-old replicated field planting of 84 ‘Ruby Red’ grapefruit trees cross-protected with three mild isolates of Citrus tristeza virus (CTV) was assessed for decline-inducing and non-decline-inducing isolates of the virus 5 years after the brown citrus aphid (BrCA) (Toxoptera citricida Kirkaldy) first was established in the experimental area. Prior to the introduction of the BrCA, the cross-protecting mild isolates had significantly reduced detectable infection with decline-inducing isolates of CTV for 16 years (average infection of 13% in cross-protected trees compared with 67% in unprotected trees). After the introduction of the BrCA, infections with decline-inducing CTV (measured by enzyme-linked immunosorbent assay) were 57, 81, and 71% for trees protected with three mild isolates, respectively, compared with 95% in unprotected trees. These results suggest that the introduction of BrCA accelerated the breakdown of cross-protection against decline-inducing isolates of CTV in grapefruit.

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