scholarly journals Germination of Phlox pilosa L. Seeds Is Improved by Gibberellic Acid and Light but Not Stratification, Potassium Nitrate, or Surface Disinfestation

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1263-1267 ◽  
Author(s):  
Angela M. Madeiras ◽  
Thomas H. Boyle ◽  
Wesley R. Autio
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Nitrate Concentration ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Promising Method ◽  
Cold Stratification ◽  
Additive Effects ◽  
Surface Sterilization ◽  
Growth Of Fungi

The effects of warm stratification and cold stratification, gibberellin-3 (GA3) concentration, potassium nitrate concentration, light, and duration of surface sterilization on the germination of downy phlox (Phlox pilosa L.) seeds were studied. Germination after 21 days (G21), days to 50% germination (T50), and number of days between 10% and 90% final germination (T90–T10) were calculated for each treatment. Total germination percentage was most significantly improved by cold stratification at 5 ± 2 °C for 10 weeks after warm stratification at 20 °C for 2 weeks; however, a substantial amount of germination occurred during the prestratification period, thus resulting in a crop with poor uniformity. A total of 10 mg·L−1 GA3 significantly improved the G21, T50, and T90–T10 values. Although GA3 concentration and duration of cold stratification period interacted significantly when the two were combined, the additive effects of GA3 and cold stratification did not significantly improve G21 values over those obtained with GA3 alone nor were T50 values improved over those obtained with cold stratification alone. Potassium nitrate did not influence the T50 and T90–T10 values and improved G21 only slightly. Light was found to be necessary for germination. Surface sterilization with 10% bleach decreased the growth of fungi on seeds but had no significant effect on the germination responses of P. pilosa seeds. Application of GA3 at 10 mg·L−1 is a promising method for improving seed germination in perennial Phlox species.

scholarly journals Use of growth regulators as priming agent for improvement of seed vigour in tomato (Lycopersicum esculentum)

2016 ◽  
Vol 8 (1) ◽  
pp. 84-87
Author(s):  
Bhim Jyoti ◽  
S. S. Gaurav ◽  
Usha Pant
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Root Length ◽  
Dry Weight ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Shoot Length ◽  
Seed Vigour

Seeds of tomato cv. Keabi were primed with of three important growth regulators viz Gibberellic acid (GA3), Napthlene acetic acid (NAA) and potassium nitrate (KNO3). Four concentration of each, Gibberellic acid and Napthlene acitic acid (25ppm, 50ppm, 75ppm.100ppm) and two concentration of KNO3 (1% and 2%) were compared with the distilled water as control. For each treatment seeds were soaked in growth regulator for 24 hrs before put for the germination test. Fifty seeds in four replications were germinated on top of paper at 200C in seed germinator for 14 days. Normally germinated seedlings were counted which gave an estimation of germination percentage. Data was recorded on the germination percentage, shoot length, seed vigour index. Most of the treatments had significant positive effect on all the quality parameters. NAA had showed adverse effect on the root length while other growth regulators were found to be significant role to improve the root length. Maximum seed germination (74%) was observed at 50 and 75ppm GA3. Highest shoot length (4.83cm) was found at 25ppm of GA3 whereas enhancement of root length occurred with the priming of 1% KNO3 (3.52cm ). Seed vigour-I, on the basis of seedling length was observed higher at 25ppm. GA3 (720) and seed vigour –II on the basis of seedling dry weight was also observed maximum priming with 100ppm GA3 (1460). From this study it was suggested that GA3 priming has important growth regulator to enhance the seed germination as well as seed vigour. Before sowing seed should be priming with GA3 for obtaining high % germination and vigorous seedling that survive under adverse condition it also increase the uniformity of field plant stand.

scholarly journals Factors Affecting Seed Dormancy and Germination of Greater Bur-Parsley (Turgenia latifolia)

2018 ◽  
Vol 36 ◽  
Author(s):  
M. REZVANI ◽  
S.A. SADATIAN ◽  
H. NIKKHAHKOUCHAKSARAEI
Keyword(s):  
Seed Germination ◽  
Environmental Factors ◽  
Seed Dormancy ◽  
Seedling Emergence ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Planting Depth ◽  
Factors Affecting ◽  
Drought And Salt Stresses

ABSTRACT: Our knowledge about seed dormancy breaking and environmental factors affecting seed germination of greater bur-parsley (Turgenia latifolia) is restricted. This study has addressed some seed dormancy breaking techniques, including different concentrations of gibberellic acid (GA3) and potassium nitrate (KNO3), leaching duration, physical scarification as well as some environmental factors effective on seed germination such as salt and drought stresses, pH and seed planting depth. Seed germination was promoted with lower concentrations of KNO3 (0.01 to 0.02 g L-1), while higher concentrations reduced germination percentage. Seed dormancy was declined by low concentrations of GA3 up to 100 ppm. Seeds of greater bur-parsley germinated in a range of pH from 3 to 7. With enhancement of drought and salt stresses, seed germination decreased. Also, there was no seed germination in a high level of stresses. Seedling emergence reduced as planting depth increased. Use of GA3, KNO3, leaching and physical scarification had a positive effect on seed dormancy breaking of greater bur-parsley. The information from the study increases our knowledge about seed dormancy breaking techniques, response of germination to drought and salt stresses and also determination of distribution regions of greater bur-parsley in the future.

scholarly journals Effect of Gibberellic Acid (GA3) and Kinetin on Seed Germination of Sesbania sesban L. and Sesbania rostrata L. (Fabaceae)

2020 ◽  
pp. 32-41
Author(s):  
S. I. Mensah ◽  
C. Ekeke ◽  
N. K. Ibeagi
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Water Absorption ◽  
Glass Container ◽  
Germination Percentage ◽  
International Institute ◽  
Tropical Agriculture ◽  
Percentage Germination ◽  
Seed Coats ◽  
Ibadan Nigeria

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.

scholarly journals Effect of pre-germinative treatments on Nothofagus glauca seed germination and seedling growth

2019 ◽  
Vol 49 ◽  
Author(s):  
Ángel Cabello ◽  
Nicolás Espinoza ◽  
Sergio Espinoza ◽  
Antonio Cabrera ◽  
Rómulo Santelices
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Growth ◽  
Iucn Red List ◽  
Cold Stratification ◽  
Natural Forests ◽  
Sowing Time ◽  
High Germination ◽  
Thiourea Solution ◽  
Quality Stock

Background: Nothofagus glauca (Phil.) Krasser (Nothofagaceae, “Hualo”) is an endemic tree of the Mediterranean zone of Chile. The natural forests in this area have been severely fragmented as a result of human causes such as replacement by agricultural crops and fast-growing tree species. From 1975, these forests have declined from 900,000 ha to 145,000 ha, so it is categorised on the IUCN Red List as ‘vulnerable’. In restoring this ecosystem, efforts should focus, in part, on the propagation of quality stock. However, information on propagation systems is still insufficient. Methods: We aimed to analyse the effect of different pre-germinative treatments and sowing times on seed germination, and seedling growth and quality. The pre-germinative treatments were: (i) cold stratification; (ii) soaking in gibberellic acid (GA3) and thiourea solution; and (iii) nursery cultivation, while the sowing times were July, August and September. Results: A high germination capacity was achieved by: soaking the seeds in GA3 solution irrespective of concentration; stratifying, irrespective of period; or soaking in 7.5 mg L-1 thiourea solution, values significantly varied from that of the control treatments. The sowing time was not relevant in terms of the percentage of germination or seedling development. Stratification at 5°C for 60 days produced the best quality indices for N. glauca seedlings but no significant differences were found in any of the morphological attributes tested as a result of the pre-germinative treatments. Conclusions: The pre-germinative treatments significantly improved the germination and seedlings growth of N. glauca. Cold stratification at 5°C for 60 days is recommended as it produced suitable seedlings for field establishment. Gibberellic acid and thiourea did not produce important effects on seedling growth. Our results suggest the presence of endogenous physiological dormancy of the N. glauca seeds. The results of this study provide important information on propagation and nursery techniques of N. glauca, which can be used in restoration programmes.

scholarly journals Methods of breaking seed dormancy for ornamental passion fruit species

2017 ◽  
Vol 23 (1) ◽  
pp. 72 ◽  
Author(s):  
Thalita Neves Marostega ◽  
Petterson Baptista Da Luz ◽  
Armando Reis Tavares ◽  
Leonarda Grillo Neves ◽  
Severino De Paiva Sobrinho
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Potassium Nitrate ◽  
Plant Production ◽  
Commercial Plant ◽  
Fruit Species ◽  
Breaking Seed Dormancy ◽  
And Control

The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high ornamental potential. This study was conducted to determine the best pre-germination treatments to overcome seed dormancy for Passiflora quadrangularis, P. nitida, P. foetida, P. eichleriana, P. alata, P. cincinnata, P. mucronata, P. micropetala, P. suberosa, P. morifolia and P. tenuifila. The experimental design was completely randomized, with five treatments and four replicates, with 25 seeds per plot. Pre-germination treatments were: seeds soaked in 1,000 mg L- 1 GA3 (gibberellic acid) for 6 hours, seeds soaked in 0.2 % KNO3 (potassium nitrate) for 24 hours, seeds soaked in 1 % KNO3 for 24 hours, partial seedcoat scarification with sandpaper number 120 and control (seeds untreated). Percentage of germination, germination velocity index and radicle length were evaluated for all species. The results showed that GA3 was effective to overcome seed dormancy in P. suberosa (86%), P. morifolia (68 %) and P. tenuifila (54%). KNO3 1% had significant effect on overcoming dormancy in seeds of P. eichleriana (66%) and scarification with sandpaper increased seed germination of P. micropetala (38%).

scholarly journals Temperature Requirements for Seed Germination of Three Penstemon Species'

HortScience ◽  
1990 ◽  
Vol 25 (2) ◽  
pp. 191-193 ◽  
Author(s):  
Phil S. Allen ◽  
Susan E. Meyer
Keyword(s):  
Seed Germination ◽  
Incubation Temperature ◽  
Marked Decrease ◽  
Rocky Mountain ◽  
Germination Percentage ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Primary Dormancy ◽  
Temperature Requirements ◽  
Seed Propagation

To determine optimum germination temperatures and effective dormancy-breaking procedures, field-grown (1983-85) seeds of `Bandera' Rocky Mountain penstemon (Penstemon strictus Benth), `Cedar' Palmer penstemon (Penstemon palmeri Gray), and firecracker penstemon (Penstemon eatonii Gray) were subjected to various cold stratification and incubation temperature treatments. Increased germination following an 8-week stratification occurred in seed lots containing dormant seeds, but a 2-week stratification generally failed to break dormancy. Older (1983) seeds of `Bandera' and `Cedar' penstemon germinated to full viability without stratification. All species showed a marked decrease in germination percentage above 20C; 15C consistently produced maximum germination after 4 weeks. At 15C, mean times to 90% of total germination were 11, 22, and 29 days for `Bandera', `Cedar', and firecracker penstemon, respectively. Transfer of seeds failing to germinate at warm temperatures (25 and 30C) to 15C and applying 720 μm gibberellic acid (GA3) solution was effective in breaking primary dormancy of firecracker penstemon and secondary dormancy of `Bandera' penstemon. Our findings suggest that incubation below 20C, combined with 8 weeks of stratification or the use of after-ripened seed, may improve seed propagation efforts for these species.

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