scholarly journals In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge

HortScience ◽  
2012 ◽  
Vol 47 (8) ◽  
pp. 1123-1128 ◽  
Author(s):  
Marija Perić ◽  
Slavica Dmitrović ◽  
Suzana Živković ◽  
Biljana Filipović ◽  
Marijana Skorić ◽  
...  
Keyword(s):  
Ex Situ Conservation ◽  
Fungal Infections ◽  
Survival Rates ◽  
Growth Conditions ◽  
Ex Situ ◽  
In Vitro Growth ◽  
Free Medium ◽  
Leaf Elongation ◽  
Primary Explants

Rindera umbellata (Waldst. & Kit.) Bunge is a rare, critically endangered and horticulturally appealing plant with unexplored pharmaceutical potential. Its distribution is restricted to sandy habitats, whereas propagation in nature is limited by fungal infections of the seeds. To initiate its ex situ conservation and provide material for metabolomic studies, we have introduced R. umbellata into in vitro culture using immature embryos as primary explants. A 72% of the embryos germinated on growth regulator-free medium. The optimization of growth conditions was based on varying carbohydrates (sucrose, glucose, and fructose) in the medium. In vitro growth and development of R. umbellata plants were significantly affected by both the type and concentration of the applied sugars. For most recorded parameters, including leaf elongation, biomass production, rooting percentage, and the number and length of roots, 0.1 m sucrose was optimal. The highest percentage of explants with developed buds was achieved on 0.06 m sucrose (38.77%) or 0.3 m glucose (27.43%). The plantlets obtained on 0.1 m sucrose were successfully acclimatized to greenhouse and field conditions with survival rates of 71.43% and 42.86%, respectively. To our best knowledge, this is the first publication dedicated to this species.

scholarly journals Cryopreservation of Woody Crops: The Avocado Case

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 934
Author(s):  
Chris O’Brien ◽  
Jayeni Hiti-Bandaralage ◽  
Raquel Folgado ◽  
Alice Hayward ◽  
Sean Lahmeyer ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Recalcitrant Seed ◽  
In Vitro Storage ◽  
Germplasm Collections ◽  
Seed Crops

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

scholarly journals Influence of in vitro growth conditions in the production of defence compounds in Mentha pulegium L.

2014 ◽  
Vol 8 ◽  
pp. 233-244 ◽  
Author(s):  
Khadija H. Benlarbi ◽  
Nouriddine Elmtili ◽  
Francisco A. Macías ◽  
Juan Carlos G. Galindo
Keyword(s):  
Growth Conditions ◽  
In Vitro Growth ◽  
Mentha Pulegium

scholarly journals Ex situ conservation of genetic resources of field elm (Ulmus minor Mill) and European white elm (Ulmus laevis Pall)

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 221-227
Author(s):  
Jelena Aleksic ◽  
Sasa Orlovic
Keyword(s):  
Genetic Resources ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
In Vitro Production ◽  
Ulmus Minor ◽  
Conservation Of Genetic Resources ◽  
Ulmus Laevis ◽  
Vitro Production

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.

Optimization of in vitro growth conditions of Pyrenophora teres for production of the phytotoxin aspergillomarasmine A

2002 ◽  
Vol 60 (3) ◽  
pp. 131-140 ◽  
Author(s):  
I. Weiergang ◽  
H.J. Lyngs JØrgensen ◽  
I.M. MØller ◽  
P. Friis ◽  
V. Smedegaard-Petersen
Keyword(s):  
Growth Conditions ◽  
Pyrenophora Teres ◽  
In Vitro Growth

Influence of in vitro growth conditions on the photosynthesis and survival of Castanea sativa plantlets during ex vitro transfer

2014 ◽  
Vol 75 (3) ◽  
pp. 625-639 ◽  
Author(s):  
Patricia L. Sáez ◽  
León A. Bravo ◽  
Mirtha I. Latsague ◽  
Marcelo J. Toneatti ◽  
Rafael E. Coopman ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
Growth Conditions ◽  
In Vitro Growth ◽  
Ex Vitro ◽  
Ex Vitro Transfer

scholarly journals Antibody Targeting the Ferritin-Like Protein Controls Listeria Infection

2010 ◽  
Vol 78 (7) ◽  
pp. 3306-3314 ◽  
Author(s):  
Walid Mohamed ◽  
Shneh Sethi ◽  
Ayub Darji ◽  
Mobarak A. Mraheil ◽  
Torsten Hain ◽  
...  
Keyword(s):  
Bacterial Load ◽  
Survival Rates ◽  
Brain Heart Infusion ◽  
Growth Conditions ◽  
Infection Process ◽  
Iron Storage ◽  
Growth Initiation ◽  
Listeria Infection

ABSTRACT The acquisition of iron during the infection process is essential for the growth of pathogenic microorganisms (S. C. Andrews, Adv. Microb. Physiol. 40:281-351, 1998; H. M. Baker, B. F. Anderson, and E. N. Baker, Proc. Natl. Acad. Sci. U. S. A. 100:3579-3583, 2003). Since the solubility of iron is low and it is toxic at low concentrations, following uptake, iron is stored in subcellular microenvironments in the iron storage protein ferritin (C. Cheers and M. Ho, J. Reticuloendothel. Soc. 34:299-309, 1983). Here, we show that ferritin-like proteins (Frl) are highly conserved in the genus Listeria and demonstrate that these proteins are present in both the cytoplasm and cell wall fractions of these bacteria. Even though Frl is expressed under different growth conditions, transcriptional mapping revealed that its regulation is complex. When bacteria are grown in brain heart infusion medium, extracellular expression involves both sigma A (SigA)- and sigma B (SigB)-dependent promoters; however, during intracellular growth, initiation of transcription is additionally SigB dependent. The expression of Frl is greatly enhanced in bacteria grown in the presence of blood, and a mutant strain lacking the frl gene was defective for growth in this medium. Using the monoclonal antibody (MAb) specific for Frl, we demonstrate that administration of anti-Frl MAb prior to infection confers antilisterial resistance in vivo, evidenced in reduced bacterial load and increased survival rates, thereby demonstrating the in vivo significance of upregulated cell surface-associated Frl expression. In vitro studies revealed that the antilisterial resistance is due to increased listerial phagocytosis.

GIS-facilitated in vitro propagation and ex situ conservation of Achillea occulta

2011 ◽  
Vol 107 (3) ◽  
pp. 531-540 ◽  
Author(s):  
Katerina Grigoriadou ◽  
Nikos Krigas ◽  
Eleni Maloupa
Keyword(s):  
In Vitro Propagation ◽  
Ex Situ Conservation ◽  
Ex Situ

scholarly journals Optimization of in vitro growth conditions for enterotropic murine coronavirus strains

1995 ◽  
Vol 52 (3) ◽  
pp. 301-307 ◽  
Author(s):  
S.R. Compton ◽  
D.F. Winograd ◽  
D.J. Gaertner
Keyword(s):  
Growth Conditions ◽  
In Vitro Growth ◽  
Murine Coronavirus

Rapid melanization of bomirski amelanotic melanoma cells in cell culture

1983 ◽  
Vol 3 (2) ◽  
pp. 189-194 ◽  
Author(s):  
A. Słominski
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Growth Conditions ◽  
Melanoma Cells ◽  
Amelanotic Melanoma ◽  
Primary Cell ◽  
In Vitro Growth

Transfer of Bomirski amelanotic melanoma ceils from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized ceils from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma.

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