scholarly journals Random Amplified Polymorphic DNA Analysis of Olive (Olea europaea L.) Cultivars

1995 ◽  
Vol 120 (3) ◽  
pp. 538-542 ◽  
Author(s):  
A. Fabbri ◽  
J.I. Hormaza ◽  
V.S. Polito
Keyword(s):  
Olea Europaea ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mediterranean Area ◽  
Polymorphic Markers ◽  
Geographic Origins ◽  
Olive Cultivars ◽  
High Degree ◽  
Olea Europaéa

Seventeen olive (Olea europaea L.) cultivars, including oil and table olive cultivars originating from throughout the Mediterranean area, were screened using random amplified polymorphic DNA (RAPD) markers. The results indicate that a high degree of polymorphism is evident in the olive germplasm reexamined. Forty random decamer primers were screened; seventeen of these produced 47 reproducible amplification fragments useful as polymorphic markers. Each of the 17 cultivars can be discriminated with a few primers. Results were analyzed for similarity among the cultivars and a cluster analysis was performed. These analyses revealed two main groups: one comprising primarily small-fruited cultivars grown mainly for oil production, and the other characterized by having large fruit. There was no apparent clustering of olive cultivars according to their geographic origins.

scholarly journals 677 PB 183 IDENTIFICATION OF OLIVE (Olea europaea L.) CULTIVARS WITH RAPD MARKERS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 529g-529 ◽  
Author(s):  
A. Fabbri ◽  
J.I. Hormaza ◽  
V.S. Polito
Keyword(s):  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Mediterranean Area ◽  
Early Results ◽  
The World ◽  
Olive Cultivars ◽  
Olea Europea ◽  
Table Olive ◽  
The Mediterranean ◽  
High Degree

We have been screening olive (Olea europea L.) cultivars using the Random Amplified Polymorphic DNA (RAPD) technique. We examined 23 olive cultivars selected to represent the important olive-growing regions of the world. These include oil and table olive cultivars originating from throughout the Mediterranean area. A high degree of polymorphisms is evident in the olive germplasm we examined. Early results indicate that polymorphisms that exist within the species are sufficient to enable efficient development of RAPD markers for distinguishing olive cultivars.

scholarly journals Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an Olive Germplasm Bank

2001 ◽  
Vol 126 (1) ◽  
pp. 64-71 ◽  
Author(s):  
A. Belaj ◽  
I. Trujillo ◽  
R. de la Rosa ◽  
L. Rallo ◽  
M.J. Giménez
Keyword(s):  
Gel Electrophoresis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Group Method ◽  
Polymorphic Markers ◽  
Germplasm Bank ◽  
Banding Patterns ◽  
Olive Cultivars

Random amplified polymorphic DNA (RAPD) analysis was performed on the main Mediterranean cultivars of olive (Olea europaea L.) from the Germplasm Bank of the Centro de Investigación y Formación Agraria “Alameda del Obispo” in Cordoba, Spain. One hundred and ninety reproducible amplification fragments were identified using 46 random primers followed by agarose gel electrophoresis. Some 63.2% of the amplification products were polymorphic, with an average of 2.6 RAPD markers obtained for each primer. The combination of polymorphic markers resulted in 244 banding patterns. The high degree of polymorphism detected made identification of all the cultivars (51) possible by combining the RAPD banding patterns of just only four primers: OPA-01, OPK-08, OPX-01, and OPX-03. Cultivar-specific RAPD markers and banding patterns were also found. A dendrogram based on unweighted pair-group method cluster analysis was constructed using a similarity matrix derived from the RAPD amplification products generated by the 46 primers. Three major groups of cultivars could be distinguished by RAPD analysis: 1) cultivars from east and northeast Spain, 2) Turkish, Syrian, and Tunisian cultivars, and 3) the majority of common olive cultivars in Spain. The dendrogram thus showed a good correlation between the banding patterns of olive cultivars and their geographic origin. A higher level of polymorphism was observed when polyacrylamide gel electrophoresis was used to separate the amplification products. Thus, adequate use of RAPD technology offers a valuable tool to distinguish between olive cultivars.

scholarly journals How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1474
Author(s):  
Sara Sion ◽  
Michele Antonio Savoia ◽  
Susanna Gadaleta ◽  
Luciana Piarulli ◽  
Isa Mascio ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Olea Europaea ◽  
Dna Analysis ◽  
Olive Tree ◽  
Olea Europaea L ◽  
Varietal Identification ◽  
Olive Cultivars ◽  
History Of ◽  
The Impact ◽  
Olea Europaéa

The olive tree (Olea europaea L.) is one of the most cultivated crops in the Mediterranean basin. Its economic importance is mainly due to the intense production of table olives and oil. Cultivated varieties are characterized by high morphological and genetic variability and present a large number of synonyms and homonyms. This necessitates the introduction of a rapid and accurate system for varietal identification. In the past, the recognition of olive cultivars was based solely on analysis of the morphological traits, however, these are highly influenced by environmental conditions. Therefore, over the years, several methods based on DNA analysis were developed, allowing a more accurate and reliable varietal identification. This review aims to investigate the evolving history of olive tree characterization approaches, starting from the earlier morphological methods to the latest technologies based on molecular markers, focusing on the main applications of each approach. Furthermore, we discuss the impact of the advent of next generation sequencing and the recent sequencing of the olive genome on the strategies used for the development of new molecular markers.

scholarly journals Characterization of Olive (Olea Europaea L.) Genetic Resources via PCR-Based Molecular Marker Systems

2021 ◽  
Vol 2 (1) ◽  
pp. 26-33
Author(s):  
S. Galatali ◽  
N. Abdul Ghafoor ◽  
E. Kaya
Keyword(s):  
Molecular Marker ◽  
Genetic Resources ◽  
Olea Europaea ◽  
Oil Quality ◽  
Mediterranean Area ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olive Varieties ◽  
Olea Europaéa

Olea europaea L., which is one of the ancient culture species cultivated in the Mediterranean area, has approximately 1200 cultivars. Its wild thype forms from O. europaea subsp. europaea var. sylvestris and its culture type forms from O. europaea subsp. europaea var. europaea. Olive cultivation is multiplied by grafting or cutting whereas wild type olive seedlings derived from seeds of O. europaea L. var. sylvestris. Because they have very big level heterozygosities, the genetic diversity of olive cultivars is quite high that they are predominantly allogamus. This genetic variability causes many confusions for identification of olive cultivars and since both oil quality and olive productivity are traits inherited to a variety, it is urgently needed to solve characterization and evaluation of olive genetic resources. The molecular marker systems are independent from environmental factors and effective technology to both detect homonymous and synonymous of cultivars and identify olive varieties. The current review is aimed to present molecular marker systems for identification and characterization of olive cultivars and emphasize their application for conservation of olive germplasm.

scholarly journals Molecular Characterization of Olive Cultivars Using RAPD Markers

2001 ◽  
Vol 126 (1) ◽  
pp. 7-12 ◽  
Author(s):  
F. Sanz-Cortés ◽  
M.L. Badenes ◽  
S. Paz ◽  
A. Íñiguez ◽  
G. Llácer
Keyword(s):  
Morphological Traits ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Fruit Size ◽  
Geographic Origin ◽  
Group Method ◽  
Polymorphic Markers ◽  
Pair Group ◽  
Olive Cultivars

Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified-polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis of their similarity values revealed two main groups divided according to geographic origin within Valencia. A third group, which included two Spanish cultivars from regions outside of Valencia, was clustered separately from the Valencian cultivars. RAPD technology proved useful in discriminating closely related cultivars. There was no apparent clustering of cultivars by fruit size or other morphological traits.

Morphology and discrimination features of pollen from Italian olive cultivars (Olea europaea L.)

Grana ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 204-214 ◽  
Author(s):  
Rita Messora ◽  
Assunta Florenzano ◽  
Paola Torri ◽  
Anna Maria Mercuri ◽  
Innocenzo Muzzalupo ◽  
...  
Keyword(s):  
Olea Europaea ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olea Europaéa

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

scholarly journals CHANGES IN FRUIT YIELD AND PHOTOSYNTHESIS PARAMETERS IN DIFFERENT OLIVE CULTIVARS (Olea europaea L.) UNDER CON-TRASTING WATER REGIMES

2020 ◽  
Vol 19 (3) ◽  
pp. 135-147
Author(s):  
Majid Golmohammadi ◽  
Omid Sofalian ◽  
Mehdi Taheri ◽  
Alireza Ghanbari ◽  
Valiollah Rasoli
Keyword(s):  
Drought Stress ◽  
Olea Europaea ◽  
Principal Component ◽  
Fruit Yield ◽  
Photosynthetic Parameters ◽  
Olive Cultivar ◽  
Drought Treatment ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olea Europaéa

The evergreen tree olive (Olea europaea L.) is the only species of the genus Olea that produces edible fruits with high ecological and economic value. This tree species has developed a series of physiochemical mechanisms to tolerate drought stress and grow under adverse climatic environments. One of these mechanisms is photosynthesis activities, so that as yet little information achieved about the relations between olive production and photosynthetic parameters under drought conditions. An experiment was carried out during two consecutive years (2015–2017) to study the response of 20 different olive tree cultivars (Olea europaea L.) to drought stress. Several parameters like net photosynthetic rate (PN), stomatal conductance (GS), transpiration rate (TE), photosynthetic pigments (total chlorophyll, chlorophyll a, b and carotenoid) and fruit yield were measured. The results of combined analysis of variance for fruit yield and other measured traits showed that year, drought treatment, cultivar main effects and their interactions were highly significant. The results indicated that drought stress reduced all traits, however GS (42.80%), PN (37.21%) and TE (37.17%) significantly affected by drought. Lower reduction in photosynthetic performance (PN, GS and TE) in the cultivar T7 compared to other olive cultivars allowed them to maintain better fruit yield. Principal component analysis (PCA) identified two PCs that accounted for 82.04 and 83.27% of the total variation in photosynthetic parameters under optimal and drought stress conditions, respectively. Taken together, mean comparison, relative changes due to drought and biplot analysis revealed that cultivars ‘T7’, ‘Roghani’, ‘Koroneiki’, ‘Korfolia’ and ‘Abou-satl’ displayed better response against drought stress. According to our results, one olive cultivar namely ‘T7’, could be used in olive breeding programs to improve new high yielding cultivars with drought tolerance for use in the drought-prone environments.

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