scholarly journals 677 PB 183 IDENTIFICATION OF OLIVE (Olea europaea L.) CULTIVARS WITH RAPD MARKERS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 529g-529 ◽  
Author(s):  
A. Fabbri ◽  
J.I. Hormaza ◽  
V.S. Polito

We have been screening olive (Olea europea L.) cultivars using the Random Amplified Polymorphic DNA (RAPD) technique. We examined 23 olive cultivars selected to represent the important olive-growing regions of the world. These include oil and table olive cultivars originating from throughout the Mediterranean area. A high degree of polymorphisms is evident in the olive germplasm we examined. Early results indicate that polymorphisms that exist within the species are sufficient to enable efficient development of RAPD markers for distinguishing olive cultivars.

1995 ◽  
Vol 120 (3) ◽  
pp. 538-542 ◽  
Author(s):  
A. Fabbri ◽  
J.I. Hormaza ◽  
V.S. Polito

Seventeen olive (Olea europaea L.) cultivars, including oil and table olive cultivars originating from throughout the Mediterranean area, were screened using random amplified polymorphic DNA (RAPD) markers. The results indicate that a high degree of polymorphism is evident in the olive germplasm reexamined. Forty random decamer primers were screened; seventeen of these produced 47 reproducible amplification fragments useful as polymorphic markers. Each of the 17 cultivars can be discriminated with a few primers. Results were analyzed for similarity among the cultivars and a cluster analysis was performed. These analyses revealed two main groups: one comprising primarily small-fruited cultivars grown mainly for oil production, and the other characterized by having large fruit. There was no apparent clustering of olive cultivars according to their geographic origins.


Caryologia ◽  
2021 ◽  
Author(s):  
Majid KHAYYATNEZHAD ◽  
Sayed Afzal Shah2

Salvia has a high degree of environmental compatibility and is widespread around the world, especially in tropical and temperate regions. It is represented by 61 species in Iran including 19 endemic species. Salvia species are mostly shrubs or subshrubs, occasionally herbs, typically perennial, sometimes biennial or annual, and often aromatic. The genus has high medicinal, commercial and horticultural value. It is the largest and one of the taxonomically complicated genus of Lamiaceae. To determine the genetic diversity and understand the species’ limits within the Iranian Salvia, we produced both morphological and molecular data using 145 randomly collected plants representing 30 species from 18 provinces of Iran. A total of 107 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 10.7 bands/primer and 44% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. reuterana. Our data depicted highest similarity between S. suffruticosa and S. hydrangea and lowest between S. aristata and S. oligphylla. Salvia limbata showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.


2007 ◽  
Vol 55 (3) ◽  
pp. 375-382 ◽  
Author(s):  
S. Mamo ◽  
A. Ayana ◽  
T. Tesso

A study on the extent and pattern of genetic variability in late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces collected from the Wello and Hararge areas of Ethiopia was conducted using random amplified polymorphic DNA (RAPD) markers for 70 individuals representing 14 populations. Four oligonucleotide primers generated a total of 55 polymorphic bands with 13–19 bands per primer and a mean of 16 bands across the 70 individuals. The value of the Shannon diversity index among the populations (0.26) and between the two regions (0.24) was low to moderate, despite the high degree of polymorphic bands per primer. The mean genetic distance (0.25) between the populations was found to be low. The low genetic variation may be due to the reduced population size of late-maturing sorghum landraces in the two regions of Ethiopia because of farmers’ decisions in the process of planting, managing, harvesting and processing their crops. Partitioning of the genetic variation into variation between and within the population revealed that 92.9% and 7.10% of the variation was found to be between and within the populations, respectively. Cluster analysis of genetic distance estimates further confirmed a low level of differentiation in late-maturing sorghum populations both between and within the regions. The implications of the results for genetic conservation purposes are discussed.


Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1765
Author(s):  
Stefano Musacchi ◽  
Ignasi Iglesias ◽  
Davide Neri

In 2018, 23.2 Mt of pears were produced in the world across 1.3 million hectares (ha) of cultivated land. This review analyzes different training systems and management styles that have been adopted worldwide, emphasizing the European pear’s economic and environmental sustainability for the Mediterranean area of cultivation. Despite a reduced number of cultivars utilized around the world, pear presents a plethora of innovative training systems. In Europe, dwarfing rootstocks have led to reduced planting distances and a subsequent increase in planting density. Still, the economic sustainability of these systems is now questionable. Many of the quince rootstocks have made it possible to considerably reduce the size of the tree and introduce the concept of continuous row planting, with the management of orchards from the ground (i.e., pedestrian orchard). The planting distance must be chosen according to the soil fertility, the vigor of the grafting combination, and the training system. The planting distance dramatically affects the pruning and the management of soil, fertilization, and irrigation. The reduction of tree size also lowers the volume of spray necessary when applying pesticides. The variability in yield worldwide results from the interaction amongst cultivar/rootstock/training system/climate/management.


2001 ◽  
Vol 126 (1) ◽  
pp. 64-71 ◽  
Author(s):  
A. Belaj ◽  
I. Trujillo ◽  
R. de la Rosa ◽  
L. Rallo ◽  
M.J. Giménez

Random amplified polymorphic DNA (RAPD) analysis was performed on the main Mediterranean cultivars of olive (Olea europaea L.) from the Germplasm Bank of the Centro de Investigación y Formación Agraria “Alameda del Obispo” in Cordoba, Spain. One hundred and ninety reproducible amplification fragments were identified using 46 random primers followed by agarose gel electrophoresis. Some 63.2% of the amplification products were polymorphic, with an average of 2.6 RAPD markers obtained for each primer. The combination of polymorphic markers resulted in 244 banding patterns. The high degree of polymorphism detected made identification of all the cultivars (51) possible by combining the RAPD banding patterns of just only four primers: OPA-01, OPK-08, OPX-01, and OPX-03. Cultivar-specific RAPD markers and banding patterns were also found. A dendrogram based on unweighted pair-group method cluster analysis was constructed using a similarity matrix derived from the RAPD amplification products generated by the 46 primers. Three major groups of cultivars could be distinguished by RAPD analysis: 1) cultivars from east and northeast Spain, 2) Turkish, Syrian, and Tunisian cultivars, and 3) the majority of common olive cultivars in Spain. The dendrogram thus showed a good correlation between the banding patterns of olive cultivars and their geographic origin. A higher level of polymorphism was observed when polyacrylamide gel electrophoresis was used to separate the amplification products. Thus, adequate use of RAPD technology offers a valuable tool to distinguish between olive cultivars.


2019 ◽  
Vol 118 (3) ◽  
pp. 654-660
Author(s):  
Marta Bellingreri

Migration and revolution in the Mediterranean area are inextricably connected. In this paper, I bring the stories of young Tunisian and Syrian revolutionaries of the 2008 and 2011 uprisings who were later forced into displacement and migration and who—both in their countries of origin and at European borders—demand freedom from their regimes’ oppression and freedom of movement. As European youth can mostly move freely in the world, Arab youth share the dream of doing the same. Both local tyrannies and their international allies, as well as unjust socioeconomic and migration policies, prevent these young people from living in dignity, from choosing where they live, and from being actors of change. The letters they share and the movement they found address an international audience and it to listen to their demands.


Phytotaxa ◽  
2015 ◽  
Vol 233 (1) ◽  
pp. 94 ◽  
Author(s):  
PEDRO PABLO FERRER-GALLEGO ◽  
Ángel Romo ◽  
Roberto Roselló ◽  
Emilio Laguna ◽  
Juan Bautista Peris

The genus Jasione Linnaeus (1753: 163) (Campanulaceae Juss.) is represented by ca. 16 species distributed throughout Europe and the Mediterranean Region, from coastal dunes to alpine zones, and growing on a wide variety of substrates as well (Sales & Hedge 2001b). The genus shows a high degree of polymorphism, which can be partially caused by its representation accross a wide range of ecological niches. This variability reaches its maximum expression within the Iberian Peninsula (Bokhari & Sales 2001).


Phytotaxa ◽  
2015 ◽  
Vol 236 (3) ◽  
pp. 296
Author(s):  
Fabrizio Bartolucci ◽  
DUILIO IAMONICO ◽  
ROBERT P. WAGENSOMMER

The genus Linum Linnaeus (1753: 277) consists of about 200 species distributed in temperate and tropical regions of the world. The Mediterranean area can be considered one of the centers of diversity of this genus (Greuter et al. 1989, Yilmaz et al. 2003, Yilmaz & Kaynak 2008, 2010, Tugay et al. 2010, Peruzzi 2011, Ruiz-Martin et al. 2015).


1983 ◽  
Vol 12 (1) ◽  
pp. 34-39 ◽  
Author(s):  
R. D. Buxton

Pine forests of the Mediterranean area are subject to very serious depredations by the caterpillar of the Pine Processionary Moth. Although there are suitable habitats in other parts of the world, the moth has so far remained within this region because of its poor power of dispersal. Traditional methods of containing it are unsatisfactory and better results are to be expected from measures designed to stabilise the population at an acceptable level rather than to eradicate it entirely.


Phytotaxa ◽  
2020 ◽  
Vol 432 (3) ◽  
pp. 296-300
Author(s):  
TIAN-CHUAN HSU ◽  
CHUN-KUEI LIAO ◽  
SHIH-WEN CHUNG ◽  
WEI-JIE HUANG

Silene Linnaeus (1753: 416) (Caryophyllaceae Juss.) is one of the largest genera of flowering plants in the world, consisting of about 700 species (Melzheimer 1988, Morton 2005) the majority of which are distributed in the Mediterranean area (Greuter 1995). The genus is critical from both taxonomic and nomenclatural points of views (see e.g., Petri et al. 2011, Rautenberg et al. 2011, Naciri et al. 2017, Đurović et al. 2018, Iamonico 2018).


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