scholarly journals Sequence Analysis of the Chalcone Synthase Gene Intron in Four Petunia Jussieu Taxa

2005 ◽  
Vol 130 (3) ◽  
pp. 360-365 ◽  
Author(s):  
R.J. Griesbach ◽  
R.M. Beck
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene ◽  
Unrooted Phylogenetic Tree ◽  
The Town ◽  
Petunia Integrifolia

The sequence of the intron within the chalcone synthase A gene (ChsA) was used to characterize Petunia integrifolia subsp. integrifolia var. depauperata (Fries) Smith et Downs, P. altiplana Ando et Hashimoto, P. littoralis Smith et Downs, and an unknown taxon from the town of Torres in Brazil. Based upon the intron, the Torres taxon most closely resembled P. integrifolia. The unrooted phylogenetic tree suggested that P. integrifolia was more closely related to P. littoralis than P. altiplana.

Cloning and sequence analysis of chalcone synthase gene in Curcuma alismatifolia

2017 ◽  
pp. 299-304
Author(s):  
S. Chanapan ◽  
B. Tontiworachai ◽  
R. Deewatthanawong ◽  
A. Suwanagul
Keyword(s):  
Sequence Analysis ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene

scholarly journals Molecular Heterogeneity of the Chalcone Synthase Intron in Petunia

HortScience ◽  
2000 ◽  
Vol 35 (7) ◽  
pp. 1347-1349 ◽  
Author(s):  
R.J. Griesbach ◽  
R.M. Beck ◽  
J.R. Stehmann
Keyword(s):  
Species Complex ◽  
Chalcone Synthase ◽  
Molecular Heterogeneity ◽  
Chain Reaction ◽  
Chalcone Synthase Gene ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Species Specific ◽  
Degree Of Heterogeneity ◽  
Petunia Integrifolia

A method was developed to characterize the genetic heterogeneity of the chalcone synthase gene intron within the Petunia integrifolia (Hook.) Schinz & Thell. species complex. The DNA from wild species collected from known locations was used to amplify the chalcone synthase gene intron through the polymerase chain reaction (PCR). The resulting PCR product was then characterized by Rsa 1 restriction, revealing a degree of heterogeneity that could be used to characterize the species genetically. Of the four different species that were characterized, two could be placed in the same genetic grouping. This study shows that the variation in the intron of the Chs A gene may be species-specific.

Molecular Evolution of Chalcone Synthase Gene Superfamily in Plants

2015 ◽  
Vol 50 (1) ◽  
pp. 55
Author(s):  
Bao Ying ◽  
Guo Changfeng ◽  
Chen Shaohua ◽  
Liu Mei
Keyword(s):  
Molecular Evolution ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

scholarly journals Multiple Sequence Alignment Reveals Diversity among Eight African Bush Mango (Irvingia gabonensis Aubry-Lecomte ex O’Rorke) Cultivars

2021 ◽  
pp. 91-96
Author(s):  
U. G. Adebo ◽  
J. O. Matthew
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Genetic Resources ◽  
Data Base ◽  
Sequence Alignment ◽  
Multiple Sequence Alignment ◽  
Multiple Sequence ◽  
Bush Mango ◽  
Irvingia Gabonensis ◽  
Cluster 2

Multiple sequence analysis is one of the most widely used model in estimating similarity among genotypes. In a bid to access useful information for the utilization of bush mango genetic resources, nucleotide sequences of eight bush mango (Irvingia gabonensis) cultivars were sourced for and retrieved form NCBI data base, and evaluated for diversity and similarity using computational biology approach. The highest alignment score (26.18), depicting the highest similarity, was between two pairs of sequence combinations; BM07:BM58 and BM12:BM69 respectively, while the least score (19.43) was between BM01: BM13. The phylogenetic tree broadly divided the cultivars into four distinct groups; BM07, BM58 (cluster one), BM01 (cluster 2), BM15, BM13 and BM35 (cluster 3), and BM12, BM69 (cluster 4), while the sequences obtained from the analysis revealed only few fully conserved regions, with the single nucleotides A, and T, which were consistent throughout the evolution. Results obtained from this study indicate that the bush mango cultivars are divergent and can be useful genetic resources for bush mango improvement through breeding.

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