scholarly journals Microsatellite Loci Transferability in Chestnut

2008 ◽  
Vol 133 (5) ◽  
pp. 692-700 ◽  
Author(s):  
Ying Wang ◽  
Ming Kang ◽  
Hongwen Huang
Keyword(s):  
Microsatellite Loci ◽  
Castanea Sativa ◽  
Breeding Programs ◽  
Genetic Studies ◽  
European Chestnut ◽  
Expected Heterozygosity ◽  
Conservation Genetic

Cross-species amplification of 55 microsatellite loci developed in european chestnut (Castanea sativa Mill.) and japanese chestnut (C. crenata Sieb & Zucc.) was tested in three chestnut species from China [C. mollissima Blume, C. seguinii Dode, and C. henryi (Skan.) Rehder & Wilson]. Among all the tested loci, 47 (85.5%), 47 (85.5%), and 44 (80%) were successfully amplified in each of the three Chinese species, respectively. All microsatellite loci tested from C. crenata successfully amplified in the Chinese species, while only 80.5%, 80.5%, and 73.2% of the loci originating from C. sativa amplified in the three Chinese species. The level of polymorphism and mean number of alleles was 58.2% and 4.12 for C. mollissima, 60% and 4.64 for C. seguinii, and 60% and 4.76 for C. henryi, with mean observed heterozygosity ranging from 0.440 to 0.549 and mean expected heterozygosity ranging from 0.506 to 0.615. Transferability of Castanea Mill. microsatellites provides a powerful tool for chestnut breeding programs and conservation genetic studies of Castanea species.

Identification of seventeen microsatellite loci for conservation genetic studies of the endemic wrasse Coris bulbifrons

2012 ◽  
Vol 5 (2) ◽  
pp. 363-366 ◽  
Author(s):  
Martin H. van der Meer ◽  
Michael G. Gardner ◽  
Michael L. Berumen ◽  
Jean-Paul A. Hobbs ◽  
Lynne van Herwerden
Keyword(s):  
Microsatellite Loci ◽  
Genetic Studies ◽  
Conservation Genetic

scholarly journals Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Agata Kostro-Ambroziak ◽  
Anna Siekiera ◽  
Magdalena Czajkowska ◽  
Jan J. Pomorski ◽  
Hanna Panagiotopoulou
Keyword(s):  
Microsatellite Loci ◽  
Population Genetic ◽  
Null Allele ◽  
Natural Populations ◽  
Multiplex Assay ◽  
Paper Wasps ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
Null Allele Frequency ◽  
Polymorphic Microsatellite

Abstract Microsatellite loci are commonly used markers in population genetic studies. In this study, we present 40 novel and polymorphic microsatellite loci elaborated for the ichneumonid parasitoid Latibulus argiolus (Rossi, 1790). Reaction condition optimisation procedures allowed 14 of these loci to be co-amplified in two PCRs and loaded in two multiplex panels onto a genetic analyser. The assay was tested on 197 individuals of L. argiolus originating from ten natural populations obtained from the host nests of paper wasps. The validated loci were polymorphic with high allele numbers ranging from eight to 27 (average 17.6 alleles per locus). Both observed and expected heterozygosity values were high, ranging between 0.75 and 0.92 for HO (mean 0.83) and from 0.70 to 0.90 for HE (mean 0.85). The optimized assay showed low genotyping error rate and negligible null allele frequency. The designed multiplex panels could be successfully applied in relatedness analyses and genetic variability studies of L. argiolus populations, which would be particularly interesting considering the coevolutionary context of this species with its social host.

Characterization of 22 microsatellite loci for conservation genetic studies of an endemic anemonefish, Amphiprion latezonatus

2014 ◽  
Vol 7 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Rosemary Steinberg ◽  
Martin van der Meer ◽  
Jean Paul Hobbs ◽  
Michael L. Berumen ◽  
Lynne van Herwerden
Keyword(s):  
Microsatellite Loci ◽  
Genetic Studies ◽  
Conservation Genetic

Identification of twenty one microsatellite loci for conservation genetic studies of the endemic butterflyfish Chaetodon tricinctus

2011 ◽  
Vol 4 (2) ◽  
pp. 243-246 ◽  
Author(s):  
Martin H. van der Meer ◽  
Michael G. Gardner ◽  
Jean-Paul A. Hobbs ◽  
Morgan Pratchett ◽  
Lynne van Herwerden
Keyword(s):  
Microsatellite Loci ◽  
Genetic Studies ◽  
Conservation Genetic

scholarly journals Microsatellite Loci for the Threatened Pearl Darter and Cross Amplification in Channel and Coal Darters

2021 ◽  
Vol 12 (1) ◽  
pp. 257-262
Author(s):  
Brian R. Kreiser ◽  
Scott R. Clark ◽  
Jacob F. Schaefer
Keyword(s):  
Microsatellite Loci ◽  
Management Decisions ◽  
Genetic Studies ◽  
River Drainage ◽  
Cross Amplification ◽  
Expected Heterozygosity ◽  
Pascagoula River ◽  
Imperiled Species ◽  
Future Management

Abstract The Pearl Darter Percina aurora is an imperiled species of fish currently only found in the Pascagoula River drainage in southern Mississippi. We tested 60 microsatellite loci identified by Illumina pair-ended sequencing. Forty of these loci were polymorphic in Pearl Darters with a mean number of alleles per locus of 11.5 and mean observed and expected heterozygosity values of 0.818 and 0.805, respectively. Eleven to 17 of these loci were also polymorphic in the closely related Channel Darter P. copelandi and Coal Darter P. brevicauda. These loci should prove useful in genetic studies associated with informing future management decisions for Pearl Darters and conservation efforts in other species of darter.

scholarly journals Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1212-1216 ◽  
Author(s):  
P. Boccacci ◽  
A. Akkak ◽  
D. Torello Marinoni ◽  
G. Bounous ◽  
R. Botta
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Castanea Sativa ◽  
Quercus Petraea ◽  
Sequence Repeat ◽  
European Chestnut ◽  
Expected Heterozygosity ◽  
Castanea Sativa Mill ◽  
Simple Sequence ◽  
The Ideal

Microsatellite or simple sequence repeat (SSR) markers show many characteristics of the ideal molecular marker, and recent studies have shown that loci developed in one species may allow analysis in taxonomically related species. In this study, 52 primer pairs developed in two oak species—Quercus robur L. and Quercus petraea (Matt.) Lieb.—were used to amplify DNA of 5 chestnut cultivars; 28 of them yielded amplicons and 12 polymorphic loci were selected and used to fingerprint 12 european chestnut (Castanea sativa Mill.) cultivars grown in the Piedmont region of northwestern Italy. The number of alleles per locus ranged from 3 to 8, mean expected heterozygosity was 0.592 (range: 0.288 to 0.868), and mean observed heterozygosity was 0.667 (range: 0.333 to 1.000). The results demonstrate the usefulness of some SSR markers isolated in Quercus for the fingerprinting and genetic mapping of Castanea cultivars.

scholarly journals Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing

2019 ◽  
Author(s):  
Daphne Amaral Fraga ◽  
Anderson Figueiredo de Carvalho ◽  
Ricardo Souza Santana ◽  
Marlon Câmara Machado ◽  
Gustavo Augusto Lacorte
Keyword(s):  
Microsatellite Loci ◽  
Population Studies ◽  
Null Alleles ◽  
Structure Population ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
Whole Genome Shotgun Sequencing ◽  
Scoring Errors ◽  
Generation Sequencing ◽  
Testing Group

AbstractTen microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main grouping were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C. purpureus testing group with clean and reproducible amplification. No evidence for scoring errors, null alleles or linkage disequilibrium was detected. Number of alleles per locus ranged from 3 to 6 and expected heterozygosity ranged from 0.78 to 0.99. These new microsatellite loci are suitable to be used in future diversity and structure population studies of C. purpureus.

scholarly journals Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

2021 ◽  
Vol 43 ◽  
pp. e51983
Author(s):  
Luiz Henrique Tolentino Santos ◽  
Cibelle Santos Dias ◽  
Lucas Amorim Silveira ◽  
Messulan Rodrigues Meira ◽  
Elisa Susilene Lisboa dos Santos ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Genetic Improvement ◽  
Prosopis Juliflora ◽  
Future Research ◽  
Resistance Gene Analogs ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
The City ◽  
Selection Of

The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.

scholarly journals A New Clonal Propagation Protocol Develops Quality Root Systems in Chestnut

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 826
Author(s):  
Patrícia Fernandes ◽  
Sara Tedesco ◽  
Inês Vieira da Silva ◽  
Carmen Santos ◽  
Helena Machado ◽  
...  
Keyword(s):  
Clonal Propagation ◽  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
Breeding Programs ◽  
Continuous Growth ◽  
European Chestnut ◽  
High Incidence ◽  
Castanea Sativa Mill ◽  
Large Production

There is, at the present time, a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European chestnut breeding programs and the production of thriving plants to restore old orchards with low yields due to a high incidence of diseases, namely root rot, is necessary. Micropropagation is a useful technique for clonal propagation. Nevertheless, in vitro culture propagation is genotype-dependent. Consequently, the existing protocols may demonstrate poor reproducibility and low efficacy. Thus, the need to contribute to the development of new micropropagation protocols suitable for large production of emerging genotypes. As a contribution to fill this gap, a three-step protocol was developed by using new combinations of Murashige & Skoog, Woody Plant, and adapted modified Melin-Norkrans media in different stages of the propagation process. About 90% of shoots were rooted, and after three months of acclimatization, 85% of these plants survived and were capable of continuous growth in the field. Currently, this protocol is being used in the production of several hybrid genotypes (with improved resistance to P. cinnamomi), selected from our ongoing breeding program and also in Castanea sativa Mill. and Castanea crenata Siebold and Zucc. species.

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