scholarly journals Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

2021 ◽  
Vol 43 ◽  
pp. e51983
Author(s):  
Luiz Henrique Tolentino Santos ◽  
Cibelle Santos Dias ◽  
Lucas Amorim Silveira ◽  
Messulan Rodrigues Meira ◽  
Elisa Susilene Lisboa dos Santos ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Genetic Improvement ◽  
Prosopis Juliflora ◽  
Future Research ◽  
Resistance Gene Analogs ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
The City ◽  
Selection Of

The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.

scholarly journals Assessing Mating Designs Utilized in Cassava Population Improvement

2021 ◽  
Author(s):  
Prince Emmanuel Norman ◽  
Daniel K. Dzidzienyo ◽  
Kumba Yannah Karim
Keyword(s):  
Genetic Improvement ◽  
Current Knowledge ◽  
Floral Biology ◽  
Genetic Studies ◽  
Relevant Stakeholder ◽  
Good Guide ◽  
Population Improvement ◽  
Or Genes ◽  
Selection Of

Cassava breeders are curious about appropriate breeding strategies utilized to generate elite genotypes with desired complimentary traits or genes from parents used in crossing. Use of appropriate mating design is influenced by a good understanding of the flower biology of the putative parent plants, type of pollination, crossing technique, pollen dissemination, the presence of male-sterility system, the purpose of the project (that is either breeding or genetic studies), and the size of population needed. The objective of this book chapter is to assess the current knowledge on mating designs, their applications and limitations in cassava improvement. This book chapter discusses the floral biology, genetic improvement, breeding procedures and mating designs in cassava. The information utilized in this study were obtained from various sources including documentary search of the journals, books and websites of relevant stakeholder organizations. Empirical findings of selected mating designs in cassava and their impacts were discussed. Findings serve as a good guide for selection of appropriate mating arrangement to obtain useful information on parents and progenies. Findings are relevant to scientists, researchers, scholars, lecturers and other relevant stakeholders.

scholarly journals Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira
Keyword(s):  
High Performance ◽  
Genetic Material ◽  
Chemical Characterization ◽  
Array Detector ◽  
Germplasm Bank ◽  
Medicinal Species ◽  
Breeding Programs ◽  
The City ◽  
Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

scholarly journals Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

2014 ◽  
Vol 34 (1) ◽  
pp. 98-102 ◽  
Author(s):  
Graziela Vieira Fonteque ◽  
Jaqueline Battilana ◽  
Ediane Paludo ◽  
Carlos André da Veiga Lima-Rosa
Keyword(s):  
Genetic Polymorphism ◽  
Polymorphic Information Content ◽  
Marker Locus ◽  
Chain Reaction ◽  
Base Pairs ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Hardy Weinberg Equilibrium ◽  
Polymerase Chain ◽  
The City

The purpose of this study was to investigate the genetic polymorphism of fifteen microsatellites loci in Brazilian (blue-egg Caipira) chickens. Samples were collected from 100 blue eggs of Caipira chickens from rural properties in the city of Dois Lajeados, RS. After DNA extraction, the fragments related to molecular markers LEI0248, LEI0221, LEI0214, LEI0192, LEI0217, LEI0254, LEI0194, LEI0212, MCW0371, ADL0278, LEI0234, MCW0183, MCW0216, MCW0330 and MCW0081 were obtained by polymerase chain reaction (PCR). The statistical analysis were carried out with the softwares ARLEQUIN 3.5 version and CERVUS 3.0.3 version. The allelic and genotypic frequencies, deviations from Hardy-Weinberg equilibrium, estimates of observed (HO) and expected (HE) heterozygosity and polymorphic information content (PIC) were obtained for each marker locus. A total of 186 alleles from 15 loci were obtained, with sizes ranging of 83 to 490 base pairs. The medium number of alleles was 12.4, the HE was 0.76±0.14 and HO was 0.49±0.21 and PIC was 0.706. The first conclusion is that the microsatellites used are polymorphic and can be used to genetic studies in chickens. The second is that the "Caipira" chicken (blue eggs) population investigated has a great genic variability, which makes than an important source of genetic resources for future animal breeding programs.

scholarly journals Advances in Genetic Improvement of Meat Quality Traits in Pig

2018 ◽  
Vol 4 (2) ◽  
pp. 177-185
Author(s):  
Yoya Vashi ◽  
Soumen Naskar ◽  
Santanu Banik ◽  
Prabhat Kumar Mandal
Keyword(s):  
Molecular Genetics ◽  
Meat Quality ◽  
Genetic Improvement ◽  
Genetic Selection ◽  
Integrated Approach ◽  
Breeding Programs ◽  
Genomics And Proteomics ◽  
Genomic Studies ◽  
Opening Up ◽  
Selection Of

This article reviews the advances in molecular genetics that have led to the genetic improvement in meat quality in pigs. During the past few decades, genes, or markers associated with genes have been identified that affects meat quality. The huge amount of information emerging from genomic studies is opening up new opportunities for the genetic selection of pigs. Several genes that influence meat quality have already been, or are close to being identified. Some of them have already been implemented into breeding programs by marker assisted selection. Fields like functional genomics and proteomics are helping in better understanding of the function and regulation of genes and how these participate in complex networks controlling the phenotypic characteristics of a trait. An integrated approach of genomics and proteomics with bioinformatics tools will further exploit the available molecular genetics information. This will allow animal breeders to make progress more rapidly to meet the demand for high quality pork.

scholarly journals Microsatellite Loci Transferability in Chestnut

2008 ◽  
Vol 133 (5) ◽  
pp. 692-700 ◽  
Author(s):  
Ying Wang ◽  
Ming Kang ◽  
Hongwen Huang
Keyword(s):  
Microsatellite Loci ◽  
Castanea Sativa ◽  
Breeding Programs ◽  
Genetic Studies ◽  
European Chestnut ◽  
Expected Heterozygosity ◽  
Conservation Genetic

Cross-species amplification of 55 microsatellite loci developed in european chestnut (Castanea sativa Mill.) and japanese chestnut (C. crenata Sieb & Zucc.) was tested in three chestnut species from China [C. mollissima Blume, C. seguinii Dode, and C. henryi (Skan.) Rehder & Wilson]. Among all the tested loci, 47 (85.5%), 47 (85.5%), and 44 (80%) were successfully amplified in each of the three Chinese species, respectively. All microsatellite loci tested from C. crenata successfully amplified in the Chinese species, while only 80.5%, 80.5%, and 73.2% of the loci originating from C. sativa amplified in the three Chinese species. The level of polymorphism and mean number of alleles was 58.2% and 4.12 for C. mollissima, 60% and 4.64 for C. seguinii, and 60% and 4.76 for C. henryi, with mean observed heterozygosity ranging from 0.440 to 0.549 and mean expected heterozygosity ranging from 0.506 to 0.615. Transferability of Castanea Mill. microsatellites provides a powerful tool for chestnut breeding programs and conservation genetic studies of Castanea species.

scholarly journals Reaction of sugarcane genotypes to brown and to orange rust by leaf whorl inoculation

2020 ◽  
Vol 50 (7) ◽  
Author(s):  
Lívia Maria Lemos Hoepers ◽  
Ana Cristina Grade Fiori-Tutida ◽  
Lucimeris Ruaro ◽  
Renata Faier Calegario ◽  
Ricardo Augusto Oliveira ◽  
...  
Keyword(s):  
Rating Scale ◽  
Brown Rust ◽  
Breeding Programs ◽  
Advanced Stages ◽  
Leaf Whorl ◽  
Moderate Resistance ◽  
Moderately Resistant ◽  
The City ◽  
Selection Of

ABSTRACT: In this research eleven sugarcane genotypes were classified in relation to their resistance to brown rust, and eleven to their resistance to orange rust. Artificial inoculation was carried out in the leaf whorl of 165-day-old plants in the city of Paranavaí, Paraná State, Brazil, in 2017. The evaluation was performed 30 days after inoculation, using a rating scale. Among the genotypes tested for brown rust, four were classified as susceptible, six as moderately susceptible and one presented moderate resistance. For orange rust, three genotypes were classified as susceptible, seven as moderately susceptible and one as moderately resistant. The evaluation and classification of the reaction of sugarcane genotypes to the rusts is an important tool that assist in preliminary trials and selection of promising genotypes for more advanced stages of breeding programs and provides information to producers on the choice of cultivars to be planted.

ESTIMASI NILAI HERITABILITAS BOBOT IKAN MAS VARIETAS PUNTEN DALAM PROGRAM SELEKSI INDIVIDU

2016 ◽  
Vol 11 (3) ◽  
pp. 217
Author(s):  
Estu Nugroho ◽  
Budi Setyono ◽  
Mochammad Su’eb ◽  
Tri Heru Prihadi
Keyword(s):  
Body Weight ◽  
Selection Response ◽  
Base Population ◽  
Breeding Programs ◽  
Male And Female ◽  
Body Weight Trait ◽  
Population Selection ◽  
Selection For ◽  
Weight Trait ◽  
Selection Of

Program pemuliaan ikan mas varietas Punten dilakukan dengan seleksi individu terhadap karakter bobot ikan. Pembentukan populasi dasar untuk kegiatan seleksi dilakukan dengan memijahkan secara massal induk ikan mas yang terdiri atas 20 induk betina dan 21 induk jantan yang dikoleksi dari daerah Punten, Kepanjen (delapan betina dan enam jantan), Kediri (tujuh betina dan 12 jantan), Sragen (27 betina dan 10 jantan), dan Blitar (15 betina dan 11 jantan). Larva umur 10 hari dipelihara selama empat bulan. Selanjutnya dilakukan penjarangan sebesar 50% dan benih dipelihara selama 14 bulan untuk dilakukan seleksi dengan panduan hasil sampling 250 ekor individu setiap populasi. Seleksi terhadap calon induk dilakukan saat umur 18 bulan pada populasi jantan dan betina secara terpisah dengan memilih berdasarkan 10% bobot ikan yang terbaik. Calon induk yang terseleksi kemudian dipelihara hingga matang gonad, kemudian dipilih sebanyak 150 pasang dan dipijahkan secara massal. Didapatkan respons positif dari hasil seleksi berdasarkan bobot ikan, yaitu 49,89 g atau 3,66% (populasi ikan jantan) dan 168,47 g atau 11,43% (populasi ikan betina). Nilai heritabilitas untuk bobot ikan adalah 0,238 (jantan) dan 0,505 (betina).Punten carp breeding programs were carried out by individual selection for body weight trait. The base population for selection activities were conducted by mass breeding of parent consisted of 20 female and 21 male collected from area Punten, eight female and six male (Kepanjen), seven female and 12 male (Kediri), 27 female and 10 male (Sragen), 15 female and 11 male (Blitar). Larvae 10 days old reared for four moths. Then after spacing out 50% of total harvest, the offspring reared for 14 months for selection activity based on the sampling of 250 individual each population. Selection of broodstock candidates performed since 18 months age on male and female populations separately by selecting based on 10% of fish with best body weight. Candidates selected broodstocks were then maintained until mature. In oder to produce the next generation 150 pairs were sets and held for mass spawning. The results revealed that selection response were positive, 49.89 g (3.66%) for male and 168.47 (11.43%) for female. Heritability for body weight is 0.238 (male) and 0.505 (female).

A Selection of Bandung City Travel Route Using The FLOYD-WARSHALL Algorithm

2020 ◽  
Vol 12 (7) ◽  
Author(s):  
Muhammad Ghifari Arfananda ◽  
◽  
Surya Michrandi Nasution ◽  
Casi Setianingsih ◽  
◽  
...  
Keyword(s):  
Traffic Congestion ◽  
Rapid Development ◽  
Route Selection ◽  
Tourist Attractions ◽  
Tourist Destinations ◽  
A Value ◽  
Distance Data ◽  
Simple Additive Weighting ◽  
The City ◽  
Selection Of

The rapid development of information and technology, the city of Bandung tourism has also increased. However, tourists who visit the city of Bandung have problems with a limited time when visiting Bandung tourist attractions. Traffic congestion, distance, and the number of tourist destinations are the problems for tourists travel. The optimal route selection is the solution for those problems. Congestion and distance data are processed using the Simple Additive Weighting (SAW) method. Route selection uses the Floyd-Warshall Algorithm. In this study, the selection of the best route gets the smallest weight with a value of 5.127 from the Algorithm process. Based on testing, from two to five tourist attractions get an average calculation time of 3 to 5 seconds. This application is expected to provide optimal solutions for tourists in the selection of tourist travel routes.

Caracterização da Peneira Média em Clones de Coffea canephora

REVISTA FIMCA ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 28-31
Author(s):  
Darlan Darlan Sanches Barbosa Alves ◽  
Victor Mouzinho Spinelli ◽  
Marcos Santana Moraes ◽  
Carolina Augusto De Souza ◽  
Rodrigo da Silva Ribeiro ◽  
...  
Keyword(s):  
Genetic Improvement ◽  
Block Design ◽  
Coffea Canephora ◽  
Coffee Production ◽  
Randomized Block Design ◽  
Significant Difference ◽  
The Mean ◽  
Four Blocks ◽  
Selection Of

Introdução: O estado de Rondônia se destaca como tradicional produtor de café, sendo o segundo maior produtor brasileiro de C. canephora. No melhoramento genético de C. canephora, a seleção de plantas de elevada peneira média está associada à bebida de qualidade superior. Objetivos: O objetivo desse estudo foi avaliar a variabilidade genética de clones de C. canephora para o tamanho dos grãos, mensurado a partir da avaliação da peneira média (PM). Materiais e Métodos: Para isso, foi conduzido ao longo de dois anos agrícolas experimento no campo experimental da Embrapa no município de Ouro Preto do Oeste-RO, para a avaliação da peneira média de 130 genótipos (clones) com características das variedades botânicas Conilon, Robusta e híbridos intervarietais. O delineamento experimental utilizado foi de blocos ao acaso, com quatro repetições de quatro plantas por parcela. Resultados: Não houve resultados significativos para a interação clones X anos, indicando uma maior consistência no comportamento das plantas ao longo do tempo. Porém foram observadas diferenças significativas para o tamanho dos grãos entre os genótipos avaliados, possibilitando selecionar genótipos superiores. Conclusão: Os genótipos agruparam-se em cinco classes de acordo com o teste de média, subsidiando a caracterização de um gradiente de variabilidade da característica avaliada ABSTRACTIntroduction: Coffea canephora accounts for approximately 35% of the world's coffee production. The state of Rondônia stands out as a traditional coffee producer, being the second largest Brazilian producer of C. canephora. In the classical genetic improvement of C. anephora, the selection of plants of high average sieve is associated with a drink of superior quality. Objectives: The objective of this udy was to evaluate the genetic variability of Coffea canephora clones for the agronomic medium sieve (PM). Materials and Methods: The experiment was conducted in the experimental field of Embrapa, municipality of OuroPreto do Oeste-RO, located at coordinates 10º44'53 "S and 62º12'57". One hundred thirty genotypes (clones) of botanical characteristics Conilon, Robusta and intervarietal hybrids were evaluated in the agricultural years 2013-2014 and 2014-2015. The experimental design was a randomized block design with four blocks and four plants per plot, spacing 3.5 x 1.5 meters between plants. Results: Significant difference was found for the grain size. According to the F test, at 5% probability, the genotypes were grouped into five classes according to the mean test. Conclusion: The results obtained subsidized the characterization of a variability gradient of the evaluated trait.

scholarly journals An Online Reputation Analysis of the Tourism Industry in Marbella: A Preliminary Study on Open Innovation

2021 ◽  
Vol 7 (2) ◽  
pp. 111
Author(s):  
María Jesús Carrasco-Santos ◽  
Antonio Manuel Ciruela-Lorenzo ◽  
Juan Gabriel Méndez Pavón ◽  
Carmen Cristófol Rodríguez
Keyword(s):  
Quantitative Research ◽  
Tourism Industry ◽  
Future Research ◽  
Full Data ◽  
Tourist Destination ◽  
Specific Advice ◽  
Sociodemographic Profile ◽  
The One ◽  
Online Reputation ◽  
The City

This research analyzed the online reputation of Marbella as a tourist destination and the profiles of the reviewers according to sociodemographic characteristics. A correlational, quantitative research technique was used in this study based on the manual extraction of more than 4000 reviews generated on TripAdvisor. The data used in this study were collected from the TripAdvisor website, taking, as a sample, tourists who had visited the city in the last three years. Ratings that did not provide full data on the variables were excluded. The findings show that Marbella is considered a luxury shopping destination. The preliminary conclusions allow us to generalize about the sociodemographic profile of its tourists. The findings of the study will provide valuable information for Marbella’s Destination Management Organization (DMO). On the one hand, this study highlights the importance of ranking the attractions of the city to create better communication strategies and enhance the appeal of those attractions that receive the best ratings, establishing the true vocation of Marbella as a tourist destination. On the other hand, it provides information on what tourists perceive to be negative elements, allowing the administration to create an improvement plan. The novelty of this research paper is that it delves into Marbella’s online reputation through an analysis of specific attractions’ ratings. Areas that require further attention in future research have been highlighted, along with specific advice on each attraction that contributes to the tourist offerings of the city.

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