scholarly journals Genetic Mapping of Chartreuse Bulb Color in Onion

2020 ◽  
Vol 145 (2) ◽  
pp. 110-119
Author(s):  
Michael J. Havey
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genetic Mapping ◽  
Allium Cepa ◽  
Genetic Map ◽  
Commercial Production ◽  
Chromosome 7 ◽  
Snp Marker ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Bulb Color

The most common bulb colors of onion (Allium cepa) are red, yellow, and white; chartreuse is a relatively rare bulb color conditioned by the homozygous recessive genotype at the G locus. In this research, plants with chartreuse bulbs were crossed with inbreds with yellow bulbs to develop segregating families for genetic mapping of the G locus. For all of 17 F2 families, segregations for yellow vs. chartreuse bulbs fit the expected 3:1 ratio (P > 0.05). DNAs were isolated from one F2 family and genotyped for single nucleotide polymorphisms (SNPs) to produce a genetic map of the G locus and 380 SNPs, of which 119 SNPs have not been previously mapped. Segregations for yellow vs. chartreuse bulbs placed the G locus at the end of chromosome 7 at 6.7 cM from the nearest SNP (isotig28625_2789). This codominant SNP marker linked to the G locus should be useful for introgression of recessive chartreuse bulb color into diverse onion populations for commercial production of this uniquely colored onion.

scholarly journals Segregations for Onion Bulb Colors Reveal That Red Is Controlled by at Least Three Loci

2008 ◽  
Vol 133 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Anil Khar ◽  
Jernej Jakse ◽  
Michael J. Havey
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Allium Cepa ◽  
Simple Sequence Repeats ◽  
Regulatory Genes ◽  
Nucleotide Polymorphisms ◽  
Anthocyanidin Synthase ◽  
Single Nucleotide ◽  
The Family ◽  
Simple Sequence ◽  
Bulb Color

Onion (Allium cepa L.) bulb color is controlled by at least five major loci (I, C, G, L, and R) and seedcoat color by one locus (B). The authors developed families segregating for bulb and seedcoat colors, simple sequence repeats (SSRs), and single nucleotide polymorphisms (SNPs) in genomic amplicons of dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). The B and C loci were linked to SSRs on chromosomes 1 and 6 respectively. For all of three families, SNPs in DFR cosegregated with the R locus conditioning red bulb color. In the family from B2246 × B11159, red bulbs versus yellow bulbs were controlled by DFR and a locus (L2) linked at 6.3 cM to ANS. The authors propose that yellow bulb onions have been independently selected numerous times and that yellow populations carry independent mutations in structural or regulatory genes controlling the production of red bulb color in onion.

scholarly journals Identification of Pyrus Single Nucleotide Polymorphisms (SNPs) and Evaluation for Genetic Mapping in European Pear and Interspecific Pyrus Hybrids

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77022 ◽  
Author(s):  
Sara Montanari ◽  
Munazza Saeed ◽  
Mareike Knäbel ◽  
YoonKyeong Kim ◽  
Michela Troggio ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genetic Mapping ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
European Pear

scholarly journals High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]

2022 ◽  
Vol 12 ◽  
Author(s):  
Hui Yan ◽  
Meng Ma ◽  
Muhammad Qadir Ahmad ◽  
Mohamed Hamed Arisha ◽  
Wei Tang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Sweet Potato ◽  
Genetic Map ◽  
Quantitative Trait ◽  
Ipomoea Batatas ◽  
Anthocyanin Content ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Positive Correlation ◽  
High Heritability

Flesh color (FC), skin color (SC), and anthocyanin content (AC) are three important traits being used for commodity evaluation in purple-fleshed sweet potato. However, to date, only a few reports are available on the inheritance of these traits. In this study, we used a biparental mapping population of 274 F1 progeny generated from a cross between a dark purple-fleshed (Xuzishu8) and white-fleshed (Meiguohong) sweet potato variety for genetic analyses. Correlation analysis showed a significant positive correlation among AC, SC, and FC. Medium-to-high heritability was observed for these traits. We detected single nucleotide polymorphisms (SNPs) by specific length amplified fragment sequencing (SLAF-seq) with the average sequencing depth of 51.72 and 25.76 for parents and progeny, respectively. Then we constructed an integrated genetic map consisting of 15 linkage groups (LGS) of sweet potato spanning on 2,233.66 cm with an average map distance of 0.71 cm between adjacent markers. Based on the linkage map, ten major quantitative trait loci (QTLs) associated to FC, SC, and AC were identified on LG12 between 0 and 64.97 cm distance, such as one QTL for SC and FC, respectively, which explained 36.3 and 45.9% of phenotypic variation; eight QTLs for AC, which explained 10.5–28.5% of the variation. These major QTLs were highly consistent and co-localized on LG12. Positive correlation, high heritability, and co-localization of QTLs on the same LG group confirm the significance of this study to establish a marker-assisted breeding program for sweet potato improvement.

Efficient Genetic Mapping of Single Nucleotide Polymorphisms Based Upon DNA Mismatch Digestion

2005 ◽  
Vol 16 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Dainis Rungis ◽  
Britta Hamberger ◽  
Yanik Bérubé ◽  
Jennifer Wilkin ◽  
Jörg Bohlmann ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genetic Mapping ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Dna Mismatch
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