In vitro acaricidal effects of ethanolic extract and its fractions of Ageratum conyzoides L. against common cattle tick, Rhipicephalus (Boophilus) annulatus

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

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In vitro Analysis of the Efficacy of Selected Commercial Acaricides on The Cattle Tick Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae)

Egyptian Journal of Veterinary Sciences ◽

10.21608/ejvs.2020.21560.1144 ◽

2020 ◽

Vol 51 (2) ◽

pp. 153-161

Author(s):

FOLUKE AKANDE ◽

OLUBUKOLA ADENUBI ◽

Ahmed GARBA

Keyword(s):

Cattle Tick ◽

Boophilus Annulatus ◽

Vitro Analysis ◽

In Vitro Analysis

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In vitro Efficacy of Verticillium lecanii and Beauveria bassiana of Commercial Source Against Cattle Tick, Rhipicephalus (Boophilus) annulatus

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2018/6.3.139.147 ◽

2018 ◽

Vol 6 (3) ◽

Cited By ~ 2

Author(s):

Shawky Mohamed Aboelhadid ◽

Samar Mahmoud Ibrahium ◽

Walid Mahmoud Arafa ◽

Lilian Nagy Maahrous ◽

Abdel-Azeem Shaban Abdel-Baki ◽

...

Keyword(s):

Beauveria Bassiana ◽

Cattle Tick ◽

Verticillium Lecanii ◽

Boophilus Annulatus ◽

Commercial Source

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Acaricidal Activity of Artemisia nilagirica Leaves Against Rhipicephalus (Boophilus) annulatus Ticks

Planta Medica ◽

10.1055/a-1161-0220 ◽

2020 ◽

Vol 86 (18) ◽

pp. 1335-1344

Author(s):

Darsana Udayan ◽

Suresh Narayanan Nair ◽

Sanis Juliet ◽

Reghu Ravindran ◽

Sunil Athalathil ◽

...

Keyword(s):

Biological Activities ◽

Ethanolic Extract ◽

Acaricidal Activity ◽

Meat Production ◽

Phytochemical Analysis ◽

Synthetic Pyrethroids ◽

Human Beings ◽

Ageratum Conyzoides ◽

Boophilus Annulatus ◽

Artemisia Nilagirica

AbstractSince time immemorial, human beings have used various parts of plants in either prevention or treatment of ailments. Plants are rich sources of secondary metabolites such as alkaloids, steroids, terpenoids, flavonoids, and phenolic compounds with a high structural diversity. Many plants/herbs with specific biological activities such as antitumor, antioxidant, anti-inflammatory, antifungal, sedative, and acaricidal activity have been reported. Artemisia nilagirica (C. B. Clarke) Pamp. (Compositae) is a plant traditionally used for insect control in the southern part of India. Previous studies have demonstrated the activity of Artemisia species against pests. The present study thus evaluates the acaricidal activity of crude ethanolic extract of A. nilagirica leaves and its fractions against Rhipicephalus (Boophilus) annulatus. Ticks are ectoparasites that transmit several protozoal, viral, and rickettsial diseases. In south India, R. (B.) annulatus is the commonly observed tick species. Control of these acarine parasites that adversely affect milk and meat production is a tough task. Chemical acaricides such as organophosphates, synthetic pyrethroids, amitraz, and ivermectin are commonly used in tick control. The high cost, environmental hazards, and development of acaricidal resistance are some of the drawbacks of these chemical acaricides. Plant-based formulations are one of the promising approaches for the control of ectoparasites. Previously, extracts from various medicinal/aromatic plants were reported for acaricidal activity from our laboratory, such as Tetrastigma leucostaphylum (Dennst.) Alston, Chassalia curviflora (Wall.) Thwaites, Jatropha curcas L., and Ageratum conyzoides Hieron. Biochemical quantification, fluorescence analysis, and primary phytochemical analysis are already reported for the ethanolic extract and its fractions of areal parts of A. nilagirica. Phytochemical characterization of ethanolic extract of A. nilagirica from Kerala, India was shown to have the presence of terpenoids, flavonoids, steroids, saponins, fixed oils and fats, tannins, and glycosides.

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In vitro study of ivermectin efficiency against the cattle tick, Rhipicephalus (Boophilus) annulatus, among cattle herds in El-Beheira, Egypt

Veterinary World ◽

10.14202/vetworld.2019.1319-1326 ◽

2019 ◽

Vol 12 (8) ◽

pp. 1319-1326 ◽

Cited By ~ 5

Author(s):

Gaber E. Batiha ◽

Ali H. El-Far ◽

Amany A. El-Mleeh ◽

Abdelwahab A. Alsenosy ◽

Eman K. Abdelsamei ◽

...

Keyword(s):

Mortality Rate ◽

In Vitro Study ◽

Aminobutyric Acid ◽

Cattle Tick ◽

Glutathione S Transferase ◽

Boophilus Annulatus ◽

Cattle Herds ◽

Cattle Farms ◽

Gst Activity

Background and Aim: Ivermectin (IVM) has been used in veterinary practice to control different parasitic infestations over the past two decades. This study aimed to re-assess the acaricidal effects of IVM, as well as to evaluate its efficacy against Rhipicephalus (Boophilus) annulatus by determining the mortality rate, γ-aminobutyric acid (GABA) level, and oxidative/ antioxidative homeostasis (malondialdehyde [MDA] levels and glutathione S-transferase [GST] activities). Materials and Methods: Adult females of Rhipicephalus (Boophilus) annulatus were picked from cattle farms in El-Beheira Governorate, Egypt. Ticks were equally allocated to seven experimental groups to assess the acaricidal potential of IVM chemotherapeutics in controlling R. (B.) annulatus. IVM was prepared at three concentrations (11.43, 17.14, and 34.28 μM of IVM). Results: Mortality rate was calculated among the treated ticks. In addition, GABA, GST, and MDA biomarker levels were monitored. The data revealed a noticeable change in GST activity, a detoxification enzyme found in R. (B.) annulatus, through a critical elevation in mortality percentage. Conclusion: IVM-induced potent acaricidal effects against R. (B.) annulatus by repressing GST activity for the initial 24 h after treatment. Collectively, this paper reports the efficacy of IVM in a field population of R. (B.) annulatus in Egypt. Keywords: acaricidal, glutathione S-transferase, ivermectin, malondialdehyde, Rhipicephalus (Boophilus) annulatus, γ-aminobutyric acid.

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TheIn VitroEffects of Aqueous and Ethanolic Extracts of the Leaves ofAgeratum conyzoides(Asteraceae) on Three Life Cycle Stages of the Parasitic NematodeHeligmosomoides bakeri(Nematoda: Heligmosomatidae)

Veterinary Medicine International ◽

10.4061/2011/140293 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

J. Wabo Poné ◽

Olivia Fossi Tankoua ◽

Jeannette Yondo ◽

Marie Claire Komtangi ◽

Mpoame Mbida ◽

...

Keyword(s):

Ethanolic Extract ◽

Larval Survival ◽

Ageratum Conyzoides ◽

Larval Stages ◽

Life Cycle Stages ◽

Ethanolic Extracts ◽

Heligmosomoides Bakeri ◽

Negative Controls ◽

Dose Dependent

A comparativein vitrostudy was carried out to determine the ovicidal and larvicidal activity of aqueous and ethanolic extracts ofAgeratum conyzoides(Asteraceae) leaves on the eggs (unembryonated and embryonated), first and second larval stages ofHeligmosomoides bakeri. Four different concentrations (0.625, 1.25, 2.5, and 3.75 mg·mL−1) of both aqueous and ethanolic extracts were tested. Distilled water and 5% tween were used as negative controls in the bioassay. In fact, they did not affect development of eggs, hatching, and larval survival. The extract activities were dose dependent. The ethanolic extract was more potent against embryonation (39.6 ± 2.9%) than the aqueous extract (53.3 ± 10.9%) at the highest concentration (3.75 mg·ml−1). Both types of extracts killed larvae. Mebendazole proved more lethal (EC50of 0.745 and 0.323 mg·mL−1, resp., for L1and L2larvae). The aqueous extracts were the least lethal (EC50of 4.76 and 2.29 mg·mL−1, resp., for L1and L2larvae). The ethanolic extracts showed intermediate activity (EC50of 1.323 and 1.511 mg·mL−1, resp., for L1and L2larvae). It is concluded that the ovicidal and larvicidal properties of aqueous and ethanolic extracts ofAgeratum conyzoidesleaves are demonstrated in this work.

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Antifungal Activity on the Strain of Lasiodiplodia theobromae and Phytochemical Study of Ageratum conyzoides and Newbouldia laevis from the Kisangani Region / DR Congo

International Journal of Pathogen Research ◽

10.9734/ijpr/2020/v5i430138 ◽

2020 ◽

pp. 1-10

Author(s):

J. T. K. Kwembe ◽

J. P. Mbula ◽

O. Onautshu ◽

P. T. Mpiana ◽

G. Haesaert

Keyword(s):

Antifungal Activity ◽

Culture Medium ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Ageratum Conyzoides ◽

Phytochemical Study ◽

Lasiodiplodia Theobromae ◽

Chemical Screening ◽

Crude Extracts

Aims: To extract, identify and evaluate in vitro the antifungal activity of the phytochemical groups of Ageratum conyzoides and Newbouldia laevis on the strain of Lasiodiplodia theobromae. Study Design: Exploitation of medicinal plants to combat the growth of L. theobromae, responsible for the decline of cocoa cultivation. Location and Duration of Studies: Faculty of Sciences, University of Kisangani, between April 2017 and February 2018. Methodology: The crude extracts of the dry leaves of A. conyzoides and N. laevis were tested (at 100 mg/mL). Potato dextrose agar was used as the culture medium. After chemical screening, abundant phytochemical groups were isolated and tested. Results: The aqueous, 95% ethanolic and ethereal crude extracts of A. conyzoides are more antifungal (respective percentages of inhibition PI: 80.74; 84.10 and 85.64%) than those of N. laevis (63.28; 72.64 and 75.23%). The minimum inhibitory concentration (MIC) of the aqueous crude extract of A. conyzoides is lower (25 mg/mL) than that of the ethanolic extract (50 mg/mL). Tannins are very abundant in A. conyzoides and in N. laevis. Saponins, sterols and terpenes are abundant in both plants. The extraction yields of tannins and saponins are respectively 20.67 and 2.43% in A. conyzoides and 10.47 and 2.38% in N. laevis. A. conyzoides contains the gallic tannins while N. laevis, the condensates and catechics. The saponins and tannins of A. conyzoides are more antifungal (respective PI: 84.40 and 54.44%) than those of N. laevis (PI: 75.56 and 32.96%). Discussion: The saponins of A. conyzoides and N. laevis are more active on the strain of L. theobromae than the tannins. Saponins are surfactants that can destabilize membrane structure of microorganisms including fungi. Conclusion: The saponins of the two plants have shown a very interesting antifungal power on the strain of L. theobromae. The identification of their active molecules is ongoing.

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In vitro evaluation of antifungal activity of Ageratum conyzoides, Basella alba and Mitracarpus villosus on the strain of Lasiodiplodia theobromae in the Kisangani Region / D R Congo

10.1101/2020.12.09.418350 ◽

2020 ◽

Author(s):

J.T.K. Kwembe ◽

M.K. Asumani ◽

O. Onautshu ◽

P.T. Mpiana ◽

G. Haesaert

Keyword(s):

Antifungal Activity ◽

Ethanolic Extract ◽

Brown Rot ◽

Ageratum Conyzoides ◽

In Vitro Evaluation ◽

Lasiodiplodia Theobromae ◽

Basella Alba ◽

Inhibitory Power ◽

High Inhibition

ABSTRACTFollowing our previous investigations relating to the in vitro evaluation of antifungal activity, this study focused on the demonstration of the inhibitory power of extracts of fresh and dry leaves of Ageratum conyzoides, Basella alba and Mitracarpus villosus on the strain of Lasiodiplodia theobromae, fungus responsible for brown rot of cocoa pod in the Kisangani region. In six repetitions on the Patato dextrose agar medium, the strain of L. theobromae was inhibited up to 68.1% by the aqueous extract of B. alba; 60.0% by the ethanolic extract of A. conyzoides and 55.2% by the ethanolic extract of M. villosus (all obtained from dry leaves). Only the fresh leaves of the aqueous (56.7%) and ethereal (51.1%) extracts of A. conyzoides showed high inhibition percentages compared to those of the extracts of the fresh leaves of B. alba and M. villosus. The extracts of the dry leaves showed high inhibition percentages followed by those of the fresh leaves and finally those of crude extracts after two days of incubation. Thus, in addition to the expected results, the plants studied are all active. These inhibitory powers could be very high for the secondary metabolites of the respective plants.

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