scholarly journals FLOW CYTOMETRIC ANALYSES ON LINEAGE-SPECIFIC CELL SURFACE ANTIGENS OF RAT BONE MARROW TO SEEK POTENTIAL MYELOTOXIC BIOMARKERS: STATUS AFTER REPEATED DOSE OF 5-FLUOROURACIL

2004 ◽  
Vol 29 (2) ◽  
pp. 101-111 ◽  
Author(s):  
Satoko KAKIUCHI ◽  
Sachiko OHARA ◽  
Shoko OGATA ◽  
Daishiro MIURA ◽  
Yoshinori KASAHARA ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Surface ◽  
Surface Antigens ◽  
Repeated Dose ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Flow Cytometric ◽  
Specific Cell Surface ◽  
Rat Bone

Monoclonal antibodies to tissue-specific cell surface antigens

1984 ◽  
Vol 33 (2) ◽  
pp. 268-281 ◽  
Author(s):  
Ann M. Carroll ◽  
Michael Zalutsky ◽  
Sam Schatten ◽  
Atul Bhan ◽  
Linda L. Perry ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Surface Antigens ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Tissue Specific ◽  
Specific Cell Surface

Identification of New Multiple Myeloma-Specific Cell Surface Antigens As Immunotherapeutic Targets

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 3252-3252
Author(s):  
Naoki Hosen ◽  
Kana Hasegawa ◽  
Yasutaka Aoyama ◽  
Hiroyoshi Ichihara ◽  
Atsuko Mugitani ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
T Cells ◽  
Cell Surface ◽  
Surface Antigens ◽  
Expression Cloning ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Car T Cells ◽  
Car T ◽  
Specific Cell Surface

Abstract Cancer-specific cell surface antigens are ideal targets for therapies using monoclonal antibodies (mAbs) and their derivatives, such as chimeric antigen receptor (CAR)-T cells. However, such antigens are not likely to remain unidentified following extensive searching by transcriptome or proteome analyses. However, we hypothesized that cancer-specific antigens formed by post-translational events, such as glycosylation, complex formation, or conformational changes, might have been missed in previous screens. Such antigens could be discovered by thoroughly searching for cancer-specific mAbs and characterizing the antigens recognized by these mAbs. To test our hypothesis, we applied this strategy to identify novel therapeutic targets specific for multiple myeloma (MM), a major hematological cancer. We first identified two MM-specific mAbs designated as MMG49 or R8H283 after screening more than 10,000 anti-MM mAb clones. Then, we identified the antigens recognized by these mAbs by an expression cloning method. Interestingly, genes identified as antigens for both mAbs were not specific to MM cells, suggesting that both mAbs recognize MM-specific epitopes formed by post-translational events. Finally, we showed that these mAbs or CAR-T cells derived from them could reduce tumor burden in MM-xenograft models, but did not damage normal hematopoietic cells. These results not only demonstrate that these mAb or CAR-T cell therapy is promising for MM, but also suggest that MM-specific immunotherapetic target antigens formed by post translational events may be still missed. Disclosures Aoyama: Alexion: Honoraria.

scholarly journals Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry

Blood ◽  
1990 ◽  
Vol 76 (9) ◽  
pp. 1739-1747 ◽  
Author(s):  
LW Terstappen ◽  
S Johnsen ◽  
IM Segers-Nolten ◽  
MR Loken
Keyword(s):  
Bone Marrow ◽  
Light Scattering ◽  
Cell Surface ◽  
Plasma Cells ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Specific Cell Surface

The low frequency of plasma cells and the lack of specific cell surface markers has been a major obstacle for a detailed characterization of plasma cells in normal human bone marrow. Multiparameter flow cytometry enabled the identification of plasma cells in normal bone marrow aspirates. The plasma cells were located in a unique position in the correlation of forward light scattering, orthogonal light scattering, and immunofluorescent-labeled CD38. The identity of the sorted cell populations was confirmed by microscopic examination of Wright's stained slides and slides stained for cytoplasmic immunoglobulin using polyclonal antibodies reactive with light chains; ie, anti-kappa fluorescein isothiocyanate and anti lambda phycoerythrin (PE). The purity of the sorted plasma cells was greater than 97% (n = 4). The average frequency of plasma cells in normal bone marrow aspirates was low--0.25% of the nucleated cells (n = 7)--but surprisingly consistent between individuals (SD = .05; range 0.14% to 0.30%). A detailed analysis showed two distinct populations of plasma cells: (1) A population relatively smaller by forward light scattering expressed CD22, CD35, and sigE and was identified as early plasma cells (ie, lymphoplasmacytoid), and (2) a population larger by forward light scattering lacked these markers and was identified as mature plasma cells. The antigenic profile of the normal plasma cells was determined in two-color immunofluorescence studies. The expression of cell surface immunoglobulin G (IgG), IgA, IgE, IgD, IgM, and the cell surface antigens CD10, CD11b, CD13, CD11c, CD14, CD15, CD16, CD19, CD22, CD20, CD33, CD35, CD45, and HLA-DR was determined on the plasma cells. A significant heterogeneity in cell surface antigen expression was observed within the plasma cell population. Unexpectedly, myeloid- specific cell surface antigens such as CD33 and CD13 and the early B- cell antigen identified by CD10 were expressed on a proportion of plasma cells. These observations imply that the association of myeloid and early B-cell markers described in multiple myeloma may not be associated with the neoplasia but is a normal phenomenon.

scholarly journals Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry

Blood ◽  
1990 ◽  
Vol 76 (9) ◽  
pp. 1739-1747 ◽  
Author(s):  
LW Terstappen ◽  
S Johnsen ◽  
IM Segers-Nolten ◽  
MR Loken
Keyword(s):  
Bone Marrow ◽  
Light Scattering ◽  
Cell Surface ◽  
Plasma Cells ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Specific Cell Surface

Abstract The low frequency of plasma cells and the lack of specific cell surface markers has been a major obstacle for a detailed characterization of plasma cells in normal human bone marrow. Multiparameter flow cytometry enabled the identification of plasma cells in normal bone marrow aspirates. The plasma cells were located in a unique position in the correlation of forward light scattering, orthogonal light scattering, and immunofluorescent-labeled CD38. The identity of the sorted cell populations was confirmed by microscopic examination of Wright's stained slides and slides stained for cytoplasmic immunoglobulin using polyclonal antibodies reactive with light chains; ie, anti-kappa fluorescein isothiocyanate and anti lambda phycoerythrin (PE). The purity of the sorted plasma cells was greater than 97% (n = 4). The average frequency of plasma cells in normal bone marrow aspirates was low--0.25% of the nucleated cells (n = 7)--but surprisingly consistent between individuals (SD = .05; range 0.14% to 0.30%). A detailed analysis showed two distinct populations of plasma cells: (1) A population relatively smaller by forward light scattering expressed CD22, CD35, and sigE and was identified as early plasma cells (ie, lymphoplasmacytoid), and (2) a population larger by forward light scattering lacked these markers and was identified as mature plasma cells. The antigenic profile of the normal plasma cells was determined in two-color immunofluorescence studies. The expression of cell surface immunoglobulin G (IgG), IgA, IgE, IgD, IgM, and the cell surface antigens CD10, CD11b, CD13, CD11c, CD14, CD15, CD16, CD19, CD22, CD20, CD33, CD35, CD45, and HLA-DR was determined on the plasma cells. A significant heterogeneity in cell surface antigen expression was observed within the plasma cell population. Unexpectedly, myeloid- specific cell surface antigens such as CD33 and CD13 and the early B- cell antigen identified by CD10 were expressed on a proportion of plasma cells. These observations imply that the association of myeloid and early B-cell markers described in multiple myeloma may not be associated with the neoplasia but is a normal phenomenon.

Sign in / Sign up

Export Citation Format

Share Document