Development of DNA Markers Suitable for Marker Assisted Selection of Three Pi Genes Conferring Resistance to Multiple Pyricularia grisea Pathotypes

Crop Science ◽  
2004 ◽  
Vol 44 (5) ◽  
pp. 1790-1798 ◽  
Author(s):  
Robert Fjellstrom ◽  
Concetta A. Conaway-Bormans ◽  
Anna M. McClung ◽  
Marco A. Marchetti ◽  
A. Robert Shank ◽  
...  
Keyword(s):  
Dna Markers ◽  
Marker Assisted Selection ◽  
Pyricularia Grisea ◽  
Selection Of

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Molecular marker-assisted selection of the ae alleles in maize

2010 ◽  
Vol 9 (2) ◽  
pp. 1074-1084
Author(s):  
F. Chen ◽  
S.W. Zhu ◽  
Y. Xiang ◽  
H.Y. Jiang ◽  
B.J. Cheng
Keyword(s):  
Molecular Marker ◽  
Marker Assisted Selection ◽  
Selection Of

scholarly journals Development of KASP Markers for Germplasm Characterization and Fingerprinting Identification of Broccoli in China

2020 ◽  
Author(s):  
Yusen Shen ◽  
Jiansheng Wang ◽  
Huifang Yu ◽  
Xiaoguang Sheng ◽  
Zhenqing Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Marker Assisted Selection ◽  
Genetic Relationships ◽  
Cost Effective ◽  
Germplasm Characterization ◽  
2D Barcode ◽  
New Type ◽  
Generation Sequencing ◽  
Kasp Markers ◽  
Selection Of

Abstract Background: Broccoli (Brassica oleracea var. italica) is a vegetable widely cultivated in China. Many new-type broccoli cultivars were bred and developed by Chinese breeders during the recent three decades. However, the broccoli cultivar nomenclature and detailed information of genetic relationships among broccoli germplasms are unclear. Results: The present study identified millions of SNPs by next-generation sequencing of 23 representative broccoli lines. Through several steps of selection, 100 SNPs were successfully converted into KASP markers, and used to evaluate the genetic diversity, genetic relationship, and population structure of 392 broccoli accessions, which represent the mainly broccoli breeding materials in China. The initial, introduced and improved accessions were well clustered, though some accessions were overlapped between groups, probably reflecting the fact that breeding activities led to genetic similarities. To make the KASP genotyping more efficient and cost-effective, 25 of the 100 KASPs were selected for fingerprinting of all accessions, and the 2D barcode contained fingerprinting information were generated for elite varieties. Conclusion: The KASP markers developed in this study provided an efficient way for germplasm characterization, DNA fingerprinting, seed purity identification, and marker-assisted selection of broccoli in China.

scholarly journals Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

2016 ◽  
Vol 135 (5) ◽  
pp. 636-642 ◽  
Author(s):  
Ya‐Ying Wang ◽  
Chien‐Hua Chen ◽  
Annika Hoffmann ◽  
Yun‐Che Hsu ◽  
Shu‐Fen Lu ◽  
...  
Keyword(s):  
Late Blight ◽  
Marker Assisted Selection ◽  
Specific Marker ◽  
Selection Of

Marker-Assisted Selection Efficiency in Populations of Finite Size

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1353-1365
Author(s):  
Laurence Moreau ◽  
Alain Charcosset ◽  
Frédéric Hospital ◽  
André Gallais
Keyword(s):  
Relative Efficiency ◽  
Marker Assisted Selection ◽  
Analytical Approach ◽  
Type I Error ◽  
Genetic Model ◽  
Finite Size ◽  
Type I ◽  
Selection Efficiency ◽  
Molecular Information ◽  
Selection Of

Abstract The efficiency of marker-assisted selection (MAS) based on an index incorporating both phenotypic and molecular information is evaluated with an analytical approach that takes into account the size of the experiment. We consider the case of a population derived from a cross between two homozygous lines, which is commonly used in plant breeding, and we study the relative efficiency of MAS compared with selection based only on phenotype in the first cycle of selection. It is shown that the selection of the markers included in the index leads to an overestimation of the effects associated with these markers. Taking this bias into account, we study the influence of several parameters, including experiment size and heritability, on MAS efficiency. Even if MAS appears to be most interesting for low heritabilities, we point out the existence of an optimal heritability (~0.2) below which the low power of quantitative trait loci detection and the bias caused by the selection of markers reduce the efficiency. In this situation, increasing the power of detection by using a higher probability of type I error can improve MAS efficiency. This approach, validated by simulations, gives results that are generally consistent with those previously obtained by simulations using a more sophisticated biological model than ours. Thus, though developed from a simple genetic model, our approach may be a useful tool to optimize the experimental means for more complex genetic situations.

Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis)

Aquaculture ◽  
2020 ◽  
Vol 517 ◽  
pp. 734783 ◽  
Author(s):  
Shuqing Zheng ◽  
Xiaoshuang Wang ◽  
Shuai Zhang ◽  
Juan Long ◽  
Wenjing Tao ◽  
...  
Keyword(s):  
Dna Markers ◽  
Marker Assisted Selection ◽  
Silurus Meridionalis
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