scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Mapping QTL for spike fertility related traits in two double haploid wheat (Triticum aestivum L.) populations

2020 ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Double Haploid ◽  
Snp Array ◽  
Triticum Aestivum L ◽  
Yield Potential ◽  
Single Nucleotide ◽  
Breeding Programs ◽  
Grain Number Per Spike ◽  
Fertility Traits ◽  
Major Qtl ◽  
Selection Of

AbstractIn breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with a higher grain number per spike (GN) and occasionally higher grain weight (GW) (main numerical components of the yield). This task could be facilitated with the use of molecular markers such us single nucleotide polymorphism (SNP). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two double haploid (DH) populations (Baguette Premium 11 x BioINTA 2002 and Baguette 19 x BioINTA 2002, BP11xB2002 and B19xB2002). Both populations were genotyped with the iSelect 90K SNP array and evaluated in four (BP11xB19) or five (B19xB2002) environments. We identify a total of 305 QTL for 14 traits, however 28 QTL for 12 traits were considered significant with an R2 > 10% and stable for being present at least in three environments. There were detected eight hotspot regions on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B were at least two major QTL sheared confident intervals. QTL on two of these regions have previously been described, but the other six regions were never observed, suggesting that these regions would be novel. The R5A1 (QSL.perg-5A, QCN.perg-5A,QGN.perg-5A) and R5A.2 (QFFTS.perg-5A, QGW.perg-5A) regions together with the QGW.perg-6B resulted in a final higher yield suggesting them to have high relevance as candidates to be used in MAS to improve yield.Author contribution statementKey message28 stable and major QTL for 12 traits associated to spike fertility, GN and GW were detected. Two regions on 5A Ch., and QGW.perg-6B showed direct pleiotropic effects on yield.

scholarly journals Diurnal Variations in Physiological Characteristics, Photoassimilates, and Total Ascorbate in Early and Late Sown Indian Wheat Cultivars under Exposure to Elevated Ozone

Atmosphere ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1568
Author(s):  
Durgesh Singh Yadav ◽  
Bhavna Jaiswal ◽  
Shashi Bhushan Agrawal ◽  
Madhoolika Agrawal
Keyword(s):  
Ascorbic Acid ◽  
Ascorbic Acid Content ◽  
Triticum Aestivum L ◽  
Diurnal Changes ◽  
Wheat Cultivars ◽  
Negative Effects ◽  
Breeding Programs ◽  
Elevated O3 ◽  
Elevated Ozone ◽  
Selection Of

Rising tropospheric ozone (O3) in the atmosphere is detrimental to crop’s productivity and is one of the reasons for a warmer climate. The present study describes diurnal changes in gaseous exchange, chlorophyll fluorescence, ascorbic acid, and photoassimilate parameters in flag leaves of four Indian wheat (Triticum aestivum L.) cultivars (two early sown and two late sown cultivars) under ambient and elevated O3 treatments, using the open-top chambers (OTCs). Results showed that the diurnal pattern of photosynthetic rate (Ps), sucrose, and ascorbic acid content varied according to changes in photosynthetically active radiation (PAR) and O3 concentrations during the daytime and were maximum between 10:00 to 12:00. The present study suggested that elevated O3 caused more negative effects on photosystem II in early sown compared to late sown cultivars. The greater loss of photosynthesis led to lower production of photoassimilates in early sown cultivars, which utilized more assimilates in ascorbic acid formation for detoxification of ROS formed due to elevated O3. This work will also help to identify the robustness of physiological machinery in different wheat cultivars under elevated levels of O3, and may be used for selection of suitable cultivars during future breeding programs.

scholarly journals Characterization of the Common japonica-Originated Genomic Regions in the High Yielding Varieties Developed from Inter-Subspecific Crosses in Rice (Oryza sativa L.)

2020 ◽  
Author(s):  
Jeonghwan Seo ◽  
So-Myeong Lee ◽  
Jae-Hyuk Han ◽  
Na-Hyun Shin ◽  
Yoon Kyung Lee ◽  
...  
Keyword(s):  
Molecular Breeding ◽  
Snp Markers ◽  
Yield Potential ◽  
Breeding Programs ◽  
Wx Gene ◽  
High Yielding Varieties ◽  
Indica And Japonica Subspecies ◽  
Genomic Regions ◽  
Further Development

The inter-subspecific crossing between indica and japonica subspecies in rice have been utilized to improve yield potential in temperate rice. In this study, a comparative study of the genomic regions in the eight high yielding varieties (HYVs) was conducted with those of the four non-HYV varieties. NGS mapping on the Nipponbare reference genome identified a total of 14 common genomic regions of japonica-originated alleles. Interestingly, the HYVs shared the japonica-originated genomic regions on the nine chromosomes, although they were developed from different breeding programs. A panel of 94 varieties was classified into four varietal groups with the 39 SNP markers from 39 genes residing the japonica-originated genomic regions and 16 additional trait-specific SNPs. As expected, the japonica originated genomic regions were present only in JAP and HYV groups with exceptions for Chr4-1 and Chr4-2. The Wx gene located within Chr6-1 was present in HYV and JAP variety groups, while the yield-related genes were conserved as indica alleles in HYVs. The japonica-originated genomic regions and alleles shared by HYVs can be employed in molecular breeding programs for further development of HYVs in rice.

scholarly journals Agronomic potential and selection of okra hybrids to obtain potential genitors

2018 ◽  
Vol 36 (1) ◽  
pp. 112-117 ◽  
Author(s):  
Gabriel M Maciel ◽  
Rafael R Finzi ◽  
Alexandre William C Marra ◽  
Fábio J Carvalho ◽  
Ana Paula O Nogueira
Keyword(s):  
Genetic Divergence ◽  
Canonical Analysis ◽  
Index Number ◽  
Clustering Methods ◽  
Germplasm Bank ◽  
Breeding Programs ◽  
Agronomic Potential ◽  
Per Se ◽  
Fruit Mass ◽  
Selection Of

ABSTRACT Evaluation of pre-commercial hybrids in a germplasm bank is essential for determining its commercial potential or its utility as a potential genitor in a breeding program. The objective of this study was to determine genetic divergence and per se behavior of 47 pre-commercial hybrids from okra germplasm bank of the Universidade Federal de Uberlândia. Precocity index (%), number of fruits (fruits per plant), average fruit mass (g) and productivity (g per plant) were evaluated. Analysis of genetic divergence was performed by multivariate analysis using Mahalanobis distance with different clustering methods (UPGMA and canonical analysis). The performance of hybrids was compared by Scott-Knott (p= 0.05). A significant genetic variability among okra hybrids was observed. UPGMA and canonical analysis grouped the hybrids similarly, being satisfactory to represent genetic divergence. Ten hybrids presented higher performance than the commercial hybrids. Among them, UFU-QB16 stood out as the most promising hybrid for being used as a potential parent in breeding programs after auto pollination.

scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Short Communication: Selection of doubled haploid lines of rainfed lowland rice in preliminary yield trial

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
Miftahur Rizqi Akbar ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Willy Bayuardi Suwarno ◽  
Sugiyanta
Keyword(s):  
Doubled Haploid ◽  
Short Communication ◽  
Lowland Rice ◽  
Yield Potential ◽  
High Yield ◽  
Agronomic Characters ◽  
Doubled Haploid Lines ◽  
Yield Trial ◽  
Rainfed Lowland ◽  
Selection Of

Abstract. Akbar MR, Purwoko BS, Dewi IS, Suwarno WB, Sugiyanta. 2019. Short Communication: Selection of doubled haploid lines of rainfed lowland rice in preliminary yield trial. Biodiversitas 20: 2796-2801. Yield trial is an important step in rice breeding program. This research was aimed at evaluating agronomic characters and selecting the best doubled haploid rainfed rice lines for next advanced yield trial. An experiment was conducted in Indramayu from March to August 2017. The research was arranged in randomized complete block design (RCBD) with three replications. Materials used were fifty-eight doubled haploid lines and two check varieties namely Ciherang and Inpari 18. The results revealed that all observed characters had broad genetic variability and high heritability. The number of productive tillers and the number of filled grains per panicle had a positive correlation and direct effect on productivity. Based on index selection, thirty lines were selected to have good agronomic characters and high yield. These lines had characteristics of plant height (85.7-124.7 cm), number of productive tillers (8.6-14.8 tillers), day to harvest (104.0-117.3 days), number of filled grains per panicle (136.7-264.7 grains), number of total grains per panicle (152.0-305.7 grains), weight of 1000 grain (21.5-31.3 g), and productivity (4.1-6.8 tons ha-1). Keywords: good agronomic trait, high yield potential, selection index

scholarly journals QTL detection of yield-related traits of cashew

2012 ◽  
Vol 12 (1) ◽  
pp. 60-66 ◽  
Author(s):  
José Jaime Vasconcelos Cavalcanti ◽  
Francisco Herbeth Costa dos Santos ◽  
Fanuel Pereira da Silva ◽  
Cássia Renata Pinheiro
Keyword(s):  
Quantitative Trait ◽  
Marker Assisted Selection ◽  
Interval Mapping ◽  
Phenotypic Variance ◽  
Qtl Detection ◽  
Total Phenotypic Variance ◽  
Breeding Programs ◽  
Multiple Qtl ◽  
Male Flowers ◽  
Selection Of

The identification of quantitative trait loci (QTL) and marker-assisted selection with a view to breeding programs have aroused great interest, including for cashew improvement. This study identified QTL for yield-related traits: nut weight, male and hermaphrodite flowers. The traits were evaluated in 71 F1 genotypes of the cross CCP 1001 x CP 96. The methods of interval mapping and multiple QTL mapping were applied to identify QTL. Eleven QTL were detected: three for nut weight, four for male flowers and four for hermaphrodite flowers. The QTL accounted for 3.79 to 12.98 % of the total phenotypic variance and had phenotypic effects of -31.81 to 34.25 %. The potential for marker-assisted selection of the QTL hf-2f and hf-3m is great and the phenotypic effects and percentage of phenotypic variation higher than of the others.

scholarly journals Selection of Chilean potato clones for tuber yield, frying quality, plant vigor and vegetative cycle

2020 ◽  
Vol 38 (2) ◽  
pp. 217-223
Author(s):  
Giovani O da Silva ◽  
Caroline M Castro ◽  
Fernanda Q Azevedo ◽  
Arione S Pereira ◽  
Agnaldo DF de Carvalho ◽  
...  
Keyword(s):  
Tuber Yield ◽  
Agricultural Research ◽  
Block Design ◽  
Yield Potential ◽  
Breeding Programs ◽  
Marketable Tuber ◽  
Plant Vigor ◽  
Randomized Complete Block Design ◽  
Vegetative Cycle ◽  
Selection Of

ABSTRACT The objective of this study was to evaluate the yield potential, frying quality, plant vigor, and vegetative cycle of Chilean potato clones, aiming to identify those with potential to become new cultivars or to be used in breeding programs. The experiments were conducted in Pelotas-RS and Canoinhas-SC, Brazil, in spring 2017. A set of 37 advanced potato clones belonging to the Agricultural Research Institute of Chile (INIA) was compared to commercial cultivars Agata, Asterix and Atlantic. A randomized complete block design with three replications of two rows with 10 plants each was used. Tuber yield, frying quality, plant vigor and vegetative cycle traits were evaluated. The data were submitted to analysis of variance, test for grouping means, and the selection differential. In this set of clones there are some presenting superior quality in relation to marketable tuber yield and frying quality, but the vegetative cycle was not shorter nor plant vigor was lower. Clones CH 27 and CH 44 were the best for marketable tuber yield and frying quality at both locations.

scholarly journals Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
KARIMA KARA ◽  
MALIKA RACHED-KANOUNI ◽  
SAMAH MNASRI ◽  
Hichem Khammar ◽  
M’BAREK BEN NACEUR
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variability ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Breeding Programs ◽  
High Level ◽  
Derived Data ◽  
Selection Of

Abstract. Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB. 2020. Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers. Biodiversitas 21: 2638-2644. The assessment of genetic diversity is a key step in evaluating the adaptation of populations to new environmental conditions, and thus in the selection of new cultivars. The present investigation aimed to assess genetic variability of bread wheat (Triticum aestivum L.) genotypes grown in Algeria. The 17 hexaploid genotypes of bread wheat were assessed using 16 molecular microsatellites of SSR (Simple Sequence Repeat). Among the 16 microsatellite markers tested, only 11 markers were the most polymorphic and reproducible. The Polymorphism Information Content (PIC) values per locus varied from 0.14 to 0.70 with an average of 0.48 and 0.49. Genetic similarity between genotypes varied from 0.27 and 0.92 with an average of 0.60. The highest genetic distance value of 0.92 has been scored between Kauz/Pastor/Fiscal and Wbll1*2/Brambling. The lowest value of 0.27 has been scored between Cham6 and Pastor/Wbll1. Genetic similarity was calculated by molecular derived data and used to produce a dendrogram. The genotypes were grouped in two clear clusters according to their origin and pedigree. The first cluster included wheat genotypes Ain Abid (local genotypes), Attila/2 Pastor, and 5119 (introduced genotypes). The second group revealed high polymorphism and was subdivided into four sub-groups. This high level of diversity revealed among the accessions of wheat, grown in Algeria could be used in breeding programs.

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