Evaluation of Target Preparation Methods for Single‐Feature Polymorphism Detection in Large Complex Plant Genomes

Crop Science ◽  
2007 ◽  
Vol 47 (S2) ◽  
Author(s):  
Michael Gore ◽  
Peter Bradbury ◽  
René Hogers ◽  
Matias Kirst ◽  
Esther Verstege ◽  
...  
BMC Genomics ◽  
2010 ◽  
Vol 11 (1) ◽  
pp. 315 ◽  
Author(s):  
Youko Horiuchi ◽  
Yoshiaki Harushima ◽  
Hironori Fujisawa ◽  
Takako Mochizuki ◽  
Masanori Kawakita ◽  
...  

2009 ◽  
Vol 131 (3) ◽  
Author(s):  
Stefan Martens ◽  
Walter Mack ◽  
Frédéric Courtade ◽  
Philippe Perdu ◽  
Juergen Wilde ◽  
...  

The trend toward 3D integration in electronic packaging requires that failure analysis procedures and target preparation methods are adapted from conventional discrete packages to these emerging packaging technologies. This paper addresses the feasibility of laser-based target preparation in 3D integrated devices, especially stacked-die packages. Various laser technologies such as ultrashort-pulse lasers, excimer lasers, and diode-pumped solid-state (DPSS) lasers with different wavelengths and pulse durations were evaluated. In particular, it was found that ultrashort-pulse lasers with pulse durations in the femtosecond range were not suitable for ablation of the molding compound (MC). Picosecond lasers were applicable with certain constraints. It was found that for MCs with high filler content, DPSS lasers with pulse durations in the nanosecond range were the best choice. For the removal of stacked silicon dies, the laser wavelength was the most important factor in artifact-free thinning. Laser cross sections through several silicon dies with remarkably small heat-affected zones were also demonstrated. The distinct removal of the MC, silicon dies, and metal interconnected with a single laser source offers new opportunities for laser-based target preparation in 3D integrated electronic packaging devices.


2007 ◽  
Vol 104 (29) ◽  
pp. 12057-12062 ◽  
Author(s):  
J. O. Borevitz ◽  
S. P. Hazen ◽  
T. P. Michael ◽  
G. P. Morris ◽  
I. R. Baxter ◽  
...  

2009 ◽  
Vol 2 (2) ◽  
Author(s):  
Travis W. Banks ◽  
Mark C. Jordan ◽  
Daryl J. Somers

2012 ◽  
Vol 18 (2) ◽  
pp. 379-384 ◽  
Author(s):  
Lorenz Lechner ◽  
Johannes Biskupek ◽  
Ute Kaiser

AbstractSpecimen quality is vital to (scanning) transmission electron microscopy (TEM) investigations. In particular, thin specimens are required to obtain excellent high-resolution TEM images. Conventional focused ion beam (FIB) preparation methods cannot be employed to reliably create high quality specimens much thinner than 20 nm. We have developed a method forin situtarget preparation of ultrathin TEM lamellae by FIB milling. With this method we are able to routinely obtain large area lamellae with coplanar faces, thinner than 10 nm. The resulting specimens are suitable for low kV TEM as well as scanning TEM. We have demonstrated atomic resolution byCs-corrected high-resolution TEM at 20 kV on a FIB milled Si specimen only 4 nm thick; its amorphous layer measuring less than 1 nm in total.


Genetics ◽  
2006 ◽  
Vol 173 (4) ◽  
pp. 2257-2267 ◽  
Author(s):  
Rong Jiang ◽  
Paul Marjoram ◽  
Justin O. Borevitz ◽  
Simon Tavaré

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