Castration-Insensitive Luminal Cells of the Proximal Prostate are Urethral in Origin

2020 ◽  
Author(s):  
Diya Binoy Joseph ◽  
Gervaise Harcourt Henry ◽  
Alicia Malewska ◽  
Nida S. Iqbal ◽  
Hannah M. Ruetten ◽  
...  
Keyword(s):  
Luminal Cells

Induction of nucleolar changes in rat luminal cells by single injection or low-dose infusion of estradiol

Steroids ◽  
1988 ◽  
Vol 51 (1-2) ◽  
pp. 123-141 ◽  
Author(s):  
D.K. Roberts ◽  
L.A. Lavia ◽  
N. Walker ◽  
K. Anderson
Keyword(s):  
Low Dose ◽  
Single Injection ◽  
Dose Infusion ◽  
Luminal Cells

2. Ultrastructural Development of the Early Rat Otocyst

1977 ◽  
Vol 86 (1_suppl) ◽  
pp. 9-28 ◽  
Author(s):  
William F. Marovitz ◽  
Khalid M. Khan ◽  
Trina Schulte
Keyword(s):  
Stages Of Development ◽  
Surface Ectoderm ◽  
Related Function ◽  
Cellular Death ◽  
Golgi Membranes ◽  
Evolutionary Advantage ◽  
Endolymphatic Duct ◽  
Development And Differentiation ◽  
Luminal Cells

The ultrastructural development and differentiation of cells forming the rat otocyst were studied from the 9th to the 13th postcoital day (PCD). The earliest stage investigated was a simple ovoid structure with a connecting stalk to the surface ectoderm. A process of programmed cellular death involving surface ectoderm, connecting stalk, and lateral otocyst wall rapidly detached the otocyst. The cells forming the otocyst were roughly columnar, the organelles were polarized; mitochondria occurred in greatest number basally and in the supranuclear area; Golgi membranes when present were supranuclear. Luminal cells had many microvilli and cilia of various lengths were detected. The shorter, incompletely formed cilia terminated in small knob-like blebs. With each day the otocysts became more complicated and the endolymphatic duct made its appearance as an evagination of the otocyst. Many more cells were seen to have cilia in various stages of development, and by the 12th PCD possibly each cell of the main otocystic cavity had a kinocilium. Growth of the otocyst due to mitosis occurred to a great extent from a single ventromedial center. Cells in mitosis, although seen at other sites, were in greatest abundance in this area; cellular involution apparently was a related function. Together the process of over-production and programmed cellular involution of supranumerary cells not lost to other causes ( e.g., environmental) may represent an evolutionary advantage.

High Density Culture of Immuno-Magnetically Separated Human Mammary Luminal Cells

1995 ◽  
pp. 75-76
Author(s):  
Catherine Clarke ◽  
Paul Monaghan ◽  
Michael J. O’Hare
Keyword(s):  
High Density ◽  
High Density Culture ◽  
Luminal Cells

scholarly journals Epithelial Phenotypes in the Developing Human Prostate

2007 ◽  
Vol 55 (9) ◽  
pp. 885-890 ◽  
Author(s):  
Guy Letellier ◽  
Marie-José Perez ◽  
Mokrane Yacoub ◽  
Pierre Levillain ◽  
Olivier Cussenot ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Growth Factor ◽  
Regulatory Function ◽  
Basal Cells ◽  
Normal Prostate ◽  
Prostate Development ◽  
Proliferation And Apoptosis ◽  
Normal Prostate Tissue ◽  
Epidermal Growth ◽  
Luminal Cells

An intermediate population has been identified among prostate glands called transiently amplifying (TA) cells, which are characterized by coexpression of basal and luminal cytokeratins (CKs), high proliferation, and lack of p27 expression. These cells are rare in the normal adult prostate and increase in pretumoral conditions, but their importance in the developing gland remains unknown. We analyzed fetal prostates for the expression of CKs (5/6, 18, 19) and factors involved in proliferation and apoptosis: p63, Ki67, p27, epidermal growth factor (EGFR), Bcl2, androgen receptor (AR). Immunostaining was performed on a tissue microarray, including 40 prostates from fetuses aged 13-42 weeks and normal prostate tissue from 10 adults. In both solid buds and the basal compartment of canalized glands, cells expressed p63, CK5/6, CK19, CK18, BCL2, EGFR and were p27 negative. Luminal cells of fetal canalized glands continue to express CK19, EGFR, and BCL2, without p27 expression. In contrast, adult epithelial luminal cells showed diffuse AR and p27 expression, without CK19, BCL2, and EGFR staining. Proliferation was high and diffuse in fetal glands and rare and restricted to basal cells in adult glands. These results indicate that most fetal epithelial prostatic cells exhibit the phenotype of TA cells, suggesting their regulatory function in prostate development.

scholarly journals Diagnosis of Prostatic Intraepithelial Neoplasia in Luminal Cells Using Raman Spectroscopy

10.5772/32684 ◽  
2012 ◽  
Author(s):  
Suneetha Devpura ◽  
Jagdish Thakur ◽  
Seema Sethi ◽  
Vaman M. ◽  
Fazlul Sarkar ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Intraepithelial Neoplasia ◽  
Prostatic Intraepithelial Neoplasia ◽  
Luminal Cells

In situ lineage tracking of human prostatic epithelial stem cell fate reveals a common clonal origin for basal and luminal cells

2011 ◽  
Vol 225 (2) ◽  
pp. 181-188 ◽  
Author(s):  
John K Blackwood ◽  
Stuart C Williamson ◽  
Laura C Greaves ◽  
Laura Wilson ◽  
Anastasia C Rigas ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Stem Cell Fate ◽  
Epithelial Stem Cell ◽  
Clonal Origin ◽  
Luminal Cells ◽  
Lineage Tracking

Abstract 2563: LSD1 maintains differentiation and survival of mammary luminal cells and suppresses invasion of luminal breast cancer cells via GATA3

2017 ◽  
Author(s):  
Xin Hu ◽  
Dongxi Xiang ◽  
Luwei Tao ◽  
Ying Xie ◽  
Yue Jin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Luminal Breast Cancer ◽  
Luminal Cells

scholarly journals Histone Demethylase KDM6A Controls the Mammary Luminal Lineage through Enzyme-Independent Mechanisms

2016 ◽  
Vol 36 (16) ◽  
pp. 2108-2120 ◽  
Author(s):  
Kyung Hyun Yoo ◽  
Sumin Oh ◽  
Keunsoo Kang ◽  
Chaochen Wang ◽  
Gertraud W. Robinson ◽  
...  
Keyword(s):  
Biological Significance ◽  
Cell Lineage ◽  
Mammary Epithelium ◽  
Luminal Cell ◽  
Mammary Tissue ◽  
Basal Cells ◽  
Functionally Impaired ◽  
Luminal Cells

Establishment of the mammary luminal cell lineage is controlled primarily by hormones and through specific transcription factors (TFs). Previous studies have linked histone methyltransferases to the differentiation of mammary epithelium, thus opening the possibility of biological significance of counteracting demethylases. We have now demonstrated an essential role for the H3K27me3 demethylase KDM6A in generating a balanced alveolar compartment. Deletion ofKdm6ain the mammary luminal cell lineage led to a paucity of luminal cells and an excessive expansion of basal cells, bothin vivoandin vitro. The inability to form structurally normal ducts and alveoli during pregnancy resulted in lactation failure. Mutant luminal cells did not exhibit their distinctive transcription factor pattern and displayed basal characteristics. The genomic H3K27me3 landscape was unaltered in mutant tissue, and support for a demethylase-independent mechanism came from mice expressing a catalytically inactive KDM6A. Mammary tissue developed normally in these mice. Chromatin immunoprecipitation sequencing (ChIP-seq) experiments demonstrated KDM6A binding to putative enhancers enriched for key mammary TFs and H3K27ac. This study demonstrated for the first time that the mammary luminal lineage relies on KDM6A to ensure a transcription program leading to differentiated alveoli. Failure to fully implement this program results in structurally and functionally impaired mammary tissue.

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