A Single Weak Cation Exchange Liquid Chromatographic Method ((WCX)HPLC/DAD) for the of Study of Five Marketed Therapeutic Monoclonal Antibodies

2021 ◽  
Author(s):  
Antonio Martínez-Ortega ◽  
Agustin Herrera ◽  
Antonio Salmerón-Garcia ◽  
Jose Cabeza ◽  
Raquel Perez-Robles ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cation Exchange ◽  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Weak Cation Exchange ◽  
Therapeutic Monoclonal Antibodies ◽  
Liquid Chromatographic

Improved rapid procedure for simultaneous determinations of hemoglobins A1a, A1b, A1c, F, C, and S, with indication for acetylation or carbamylation by cation-exchange liquid chromatography

1993 ◽  
Vol 39 (12) ◽  
pp. 2514-2518 ◽  
Author(s):  
J W Brunnekreeft ◽  
H H Eidhof
Keyword(s):  
Liquid Chromatography ◽  
Blood Glucose ◽  
Cation Exchange ◽  
Glucose Control ◽  
Clinical Utility ◽  
Chromatographic Method ◽  
Screening Method ◽  
Blood Glucose Control ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract Although the clinical utility of glycohemoglobin (HbA1c) as an indicator of blood glucose control is generally accepted, the use of cation-exchange liquid chromatography for its quantification is controversial, given numerous studies that show no correlation between HbA1c and other indicators of blood glucose control in uremic subjects. We report a specific cation-exchange liquid-chromatographic method that measures simultaneously HbA1a, HbA1b, HbA1c, HbF, HbA0, HbC, and HbS quickly and reproducibly. Carbamylation or acetylation is indicated by extra peaks. Carbamylated hemoglobin increased the result for HbA1c by as much as 1% above the nonuremic value. The method is a fast (approximately 15 min per sample), easy to perform, reproducible, and sensitive screening method for abnormal hemoglobins. Within-assay CVs were < 0.7% in all cases and between-assay CVs were < 1.4%.

Liquid Chromatographic Fluorescence Method for the Determination of Thiabendazole Residues in Green Bananas and Banana Pulp

1994 ◽  
Vol 77 (3) ◽  
pp. 710-713 ◽  
Author(s):  
Rene V Arenas ◽  
Nelson A Johnson
Keyword(s):  
Column Liquid Chromatography ◽  
Cation Exchange ◽  
Chromatographic Method ◽  
Solid Phase ◽  
Extraction Column ◽  
Phase Extraction ◽  
Liquid Chromatographic Method ◽  
Cation Exchange Column ◽  
Liquid Chromatographic

Abstract A novel liquid chromatographic method was developed for the determination of thiabendazole (TBZ) residues in whole green bananas and ripe banana pulp. TBZ is extracted from the banana matrix with ethyl acetate, followed by cleanup of extract on a cation-exchange, solid-phase extraction column. The extract is analyzed for TBZ residues by column liquid chromatography using a cation-exchange column with fluorescence detection. Recoveries of TBZ from whole green bananas fortified with TBZ at 0.05-10 ppm and from ripe banana pulp fortified with TBZ at 0.01-2 ppm averaged 93 and 95%, respectively. The following method for monitoring TBZ residues in whole green bananas and ripe banana pulp is simple, rapid, and sensitive.

Assessment of liquid chromatographic method robustness by use of Plackett-Burman design

2010 ◽  
Vol 22 (2) ◽  
pp. 281-296 ◽  
Author(s):  
M. Mašković ◽  
B. Jančić-Stojanović ◽  
A. Malenović ◽  
D. Ivanović ◽  
M. Medenica
Keyword(s):  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Burman Design ◽  
Liquid Chromatographic ◽  
Plackett Burman Design
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