Quantitative ELISA for SERPINA4/kallistatin

Background: SERPINA4/kallistatin is a multifunctional protein expressed from the liver; its concentration in blood circulation reflects the degree of liver dysfunction and may serve as a diagnostic/prognostic biomarker for chronic liver diseases (CLD). Materials & methods: Antibodies specific for SERPINA4/kallistatin were used for the development of an enzyme-linked immunosorbent assay (ELISA). For correlative studies, blood samples from patients with cirrhotic liver and healthy patients were collected from R.L. Jalappa Hospital & Research Centre, Kolar. Results: Interference of other SERPINs was ruled out using western blot analysis. Quantitative ELISA was developed using monospecific antibodies as capture antibodies. Conclusion: The accuracy of the developed ELISA was determined by inter- and intra-assay precision. Linearity was defined using a spiked sample with serial dilutions. Reduced levels of SERPINA4/kallistatin were observed in patients with CLD compared with healthy controls.

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Cryptosporidiosis in patients with diarrhea and chronic liver diseases

The Journal of Infection in Developing Countries ◽

10.3855/jidc.5166 ◽

2014 ◽

Vol 8 (12) ◽

pp. 1584-1590 ◽

Cited By ~ 4

Author(s):

Nasser Mousa ◽

Ahmed Abdel-Razik ◽

Hala El-Nahas ◽

Atef El-Shazly ◽

Mohammad Abdelaziz ◽

...

Keyword(s):

Hepatic Encephalopathy ◽

Liver Diseases ◽

Meld Score ◽

Chronic Liver ◽

Enzyme Linked Immunosorbent Assay ◽

Cryptosporidium Infection ◽

Chronic Liver Diseases ◽

Pugh Class ◽

Class C ◽

Precipitating Factor

Introduction: The aim of this study was to evaluate the epidemiology and clinical significance of Cryptosporidium in patients with diarrhea and chronic liver diseases. Methodology: The study included 150 patients with chronic liver diseases and diarrhea, and 50 subjects with diarrhea as a control group. Stool samples were screened for the presence of Cryptosporidium by microscopic examination after modified Ziehl-Neelsen staining and detection of Cryptosporidium coproantigen by enzyme-linked immunosorbent assay (ELISA). Results: The prevalence of Cryptosporidium infection in patients with chronic liver diseases was 30% (45/150) versus 14% (7/50) in controls. Cryptosporidium infection increased with the progression of chronic liver diseases from Child-Pugh class A to Child-Pugh class C (p< 0.001) and from model for end-stage liver disease (MELD) score ≤ 9 to MELD score > 9 (p< 0.031). Nine patients in Child-Pugh class C with diarrhea associated with Cryptosporidium infection developed hepatic encephalopathy, and only diarrhea was identified as a precipitating factor for hepatic encephalopathy. Conclusions: Cryptosporidium is one of the important causes of diarrhea in patients with chronic liver diseases. The infection significantly increased with the progression of chronic liver diseases. In patients with advanced chronic liver diseases, Cryptosporidium infection may be a precipitating factor of hepatic encephalopathy.

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Prevalence and Clinical Significance of Immunoglobulin A Antibodies against Tissue Transglutaminase in Patients with Diverse Chronic Liver Diseases

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.12.8.941-948.2005 ◽

2005 ◽

Vol 12 (8) ◽

pp. 941-948 ◽

Cited By ~ 15

Author(s):

Anastasios E. Germenis ◽

Efthalia E. Yiannaki ◽

Kalliopi Zachou ◽

Violeta Roka ◽

Sotirios Barbanis ◽

...

Keyword(s):

Liver Disease ◽

Clinical Significance ◽

Liver Diseases ◽

Tissue Transglutaminase ◽

Immunoglobulin A ◽

Chronic Liver ◽

Intestinal Biopsy ◽

Enzyme Linked Immunosorbent Assay ◽

Chronic Liver Diseases ◽

Hla Dq

ABSTRACT The prevalence of celiac disease (CD) and the prevalence and clinical significance of anti-tissue transglutaminase (tTG) antibodies (tTGAbs) in a large series of patients with chronic liver diseases were assessed. We studied 738 patients (462 with chronic viral hepatitis, 117 with autoimmune liver diseases, 113 with alcoholic or nonalcoholic fatty liver disease, and 46 with other liver disorders) and 1,350 healthy controls (HC). Immunoglobulin A (IgA) tTGAbs were measured by enzyme-linked immunosorbent assay and a microsphere-based flow cytometric assay. Positive sera were investigated for IgA antiendomysial antibodies (EmA). IgA tTGAb-positive subjects were invited to undergo a small-intestinal biopsy and HLA-DQ allele typing. Four of 1,350 HC (0.3%) tested tTGAb+ EmA+ and underwent a biopsy (CD confirmation in all). Four of 738 liver disease patients tested tTGAbs+ EmA+ (0.54%; not statistically significant). Two were HCV infected (1.24%; not statistically significant), and two had transaminasemia of unknown origin. Forty-three patients tested tTGAbs+ EmA− (5.8%; P < 0.001 compared to HC). Inhibition experiments verified the existence of specific IgA anti-tTG reactivity. Twenty-six of 43 patients underwent a biopsy (all negative for CD). Binary logistic regression analysis revealed age (P = 0.008), cirrhosis (P = 0.004), alkaline phosphatase (P = 0.026), and antinuclear antibodies (P = 0.012) as independent risk factors for tTGAb reactivity among the patients. It was concluded that CD prevalence is the same in HC and patients with chronic liver diseases. The prevalence of tTGAbs is higher in hepatic patients compared to HC, but their specificity for CD diagnosis in this group of patients is low. tTGAbs in patients appear to be associated with the presence of autoimmunity, cirrhosis, and cholestasis, irrespective of the origin of the liver disease.

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Immune Responses to Bile-Tolerant Helicobacter Species in Patients with Chronic Liver Diseases, a Randomized Population Group, and Healthy Blood Donors

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.6.1160-1164.2002 ◽

2002 ◽

Vol 9 (6) ◽

pp. 1160-1164 ◽

Cited By ~ 11

Author(s):

Olga Ananieva ◽

Ingrid Nilsson ◽

Tamara Vorobjova ◽

Raivo Uibo ◽

Torkel Wadström

Keyword(s):

Cell Surface ◽

Liver Diseases ◽

Blood Donors ◽

Population Group ◽

Cross Reactivity ◽

Chronic Liver ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Chronic Liver Diseases ◽

H Pylori

ABSTRACT Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (ELISA). The results were compared with the antibody responses to Helicobacter pylori. For analysis of a possible cross-reactivity between bile-tolerant Helicobacter species and H. pylori, sera from a subpopulation of each group were absorbed with a whole-cell extract of H. pylori and retested by ELISA. Results before absorption showed that the mean value of the ELISA units for H. pullorum was significantly higher in patients with CLD than in healthy blood donors (P = 0.01). Antibody reactivity to cell surface protein of H. hepaticus was also significantly higher in the CLD patients than in the healthy blood donors and the population group (P = 0.005 and P = 0.002, respectively). Following the absorption, antibody responses to H. pullorum decreased significantly in all three groups (P = 0.0001 for CLD patients, P = 0.0005 for the population group, and P < 0.0001 for the blood donors), indicating that cross-reactivity between H. pylori and other Helicobacter spp. occurs. The antibody responses to H. hepaticus and H. bilis in CLD patients remained high following absorption experiments compared to ELISA results before absorption. The significance of this finding requires further investigations.

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Detection of Anti-LKM-1(Anti-CYP2D6) by an: Enzyme-Linked Immunosorbent Assay in Adult Patients with Chronic Liver Diseases

Autoimmunity ◽

10.3109/08916939908994768 ◽

1999 ◽

Vol 30 (2) ◽

pp. 107-114 ◽

Cited By ~ 13

Author(s):

Hiroshi Miyakawa ◽

Eriko Kikazawa ◽

Kazuhiro Abe ◽

Kentaro Kikuchi ◽

Hirotoshi Fujikawa ◽

...

Keyword(s):

Immunosorbent Assay ◽

Liver Diseases ◽

Chronic Liver ◽

Adult Patients ◽

Enzyme Linked Immunosorbent Assay ◽

Chronic Liver Diseases

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A sensitive enzyme-linked immunosorbent assay for anti-recombinant interferon-.ALPHA. 2a antibodies in chronic liver diseases.

Kanzo ◽

10.2957/kanzo.30.381 ◽

1989 ◽

Vol 30 (3) ◽

pp. 381-382

Author(s):

Yusei IKEDA ◽

Kazuhiko MIYAKE ◽

Hiroko ARAI ◽

Masami YAMANAKA ◽

Haruki YAMADA ◽

...

Keyword(s):

Interferon Alpha ◽

Immunosorbent Assay ◽

Liver Diseases ◽

Chronic Liver ◽

Enzyme Linked Immunosorbent Assay ◽

Chronic Liver Diseases ◽

Recombinant Interferon ◽

Recombinant Interferon Alpha

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Histidine-rich glycoprotein as a prognostic biomarker for sepsis

Scientific Reports ◽

10.1038/s41598-021-89555-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Kosuke Kuroda ◽

Kenzo Ishii ◽

Yuko Mihara ◽

Naoya Kawanoue ◽

Hidenori Wake ◽

...

Keyword(s):

Predictive Power ◽

Prognostic Biomarker ◽

Enzyme Linked Immunosorbent Assay ◽

Newly Diagnosed ◽

Mice Model ◽

Icu Patients ◽

Blood Samples ◽

Mortality Hazard ◽

Mortality Hazard Ratio ◽

Previous Clinical Study

AbstractVarious biomarkers have been proposed for sepsis; however, only a few become the standard. We previously reported that plasma histidine-rich glycoprotein (HRG) levels decreased in septic mice, and supplemental infusion of HRG improved survival in mice model of sepsis. Moreover, our previous clinical study demonstrated that HRG levels in septic patients were lower than those in noninfective systemic inflammatory response syndrome patients, and it could be a biomarker for sepsis. In this study, we focused on septic patients and assessed the differences in HRG levels between the non-survivors and survivors. We studied ICU patients newly diagnosed with sepsis. Blood samples were collected within 24 h of ICU admission, and HRG levels were determined using an enzyme-linked immunosorbent assay. Ninety-nine septic patients from 11 institutes in Japan were included. HRG levels were significantly lower in non-survivors (n = 16) than in survivors (n = 83) (median, 15.1 [interquartile ranges, 12.7–16.6] vs. 30.6 [22.1–39.6] µg/ml; p < 0.01). Survival analysis revealed that HRG levels were associated with mortality (hazard ratio 0.79, p < 0.01), and the Harrell C-index (predictive power) for HRG was 0.90. These results suggested that HRG could be a novel prognostic biomarker for sepsis.

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