scholarly journals Challenges and strategy in treatment with exosomes for cell-free-based tissue engineering in dentistry

2021 ◽  
Author(s):  
Ika Dewi Ana ◽  
Anggraini Barlian ◽  
Atik Choirul Hidajah ◽  
Christofora Hanny Wijaya ◽  
Hari Basuki Notobroto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Large Scale ◽  
Clinical Applications ◽  
Regenerative Therapy ◽  
Research Needs ◽  
Dental Tissue ◽  
Alternative Approach ◽  
Regenerative Dentistry ◽  
Dental Tissue Engineering

In dentistry, problems of craniofacial, osteochondral, periodontal tissue, nerve, pulp or endodontics injuries, and osteoarthritis need regenerative therapy. The use of stem cells in dental tissue engineering pays a lot of increased attention, but there are challenges for its clinical applications. Therefore, cell-free-based tissue engineering using exosomes isolated from stem cells is regarded an alternative approach in regenerative dentistry. However, practical use of exosome is restricted by limited secretion capability of cells. For future regenerative treatment with exosomes, efficient strategies for large-scale clinical applications are being studied, including the use of ceramics-based scaffold to enhance exosome production and secretion which can resolve limited exosome secretory from the cells when compared with the existing methods available. Indeed, more research needs to be done on these strategies going forward.

scholarly journals From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Anne Baudry ◽  
Emel Uzunoglu ◽  
Benoit Schneider ◽  
Odile Kellermann ◽  
Michel Goldberg
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Tissue ◽  
Dental Tissue Engineering ◽  
Tooth Repair

scholarly journals Development of Synthetic and Natural Materials for Tissue Engineering Applications Using Adipose Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Yunfan He ◽  
Feng Lu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Regeneration ◽  
Large Scale ◽  
Clinical Applications ◽  
Adipose Stem Cells ◽  
Laboratory Research ◽  
Engineering Applications ◽  
Plastic And Reconstructive Surgery ◽  
Relative Ease

Adipose stem cells have prominent implications in tissue regeneration due to their abundance and relative ease of harvest from adipose tissue and their abilities to differentiate into mature cells of various tissue lineages and secrete various growth cytokines. Development of tissue engineering techniques in combination with various carrier scaffolds and adipose stem cells offers great potential in overcoming the existing limitations constraining classical approaches used in plastic and reconstructive surgery. However, as most tissue engineering techniques are new and highly experimental, there are still many practical challenges that must be overcome before laboratory research can lead to large-scale clinical applications. Tissue engineering is currently a growing field of medical research; in this review, we will discuss the progress in research on biomaterials and scaffolds for tissue engineering applications using adipose stem cells.

scholarly journals The Neurovascular Properties of Dental Stem Cells and Their Importance in Dental Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Jessica Ratajczak ◽  
Annelies Bronckaers ◽  
Yörg Dillen ◽  
Pascal Gervois ◽  
Tim Vangansewinkel ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Growth ◽  
Deciduous Teeth ◽  
Human Tooth ◽  
Dental Stem Cells ◽  
Dental Tissue ◽  
Periodontal Ligament Stem Cells ◽  
Essential Components ◽  
Dental Tissue Engineering

Within the field of tissue engineering, natural tissues are reconstructed by combining growth factors, stem cells, and different biomaterials to serve as a scaffold for novel tissue growth. As adequate vascularization and innervation are essential components for the viability of regenerated tissues, there is a high need for easily accessible stem cells that are capable of supporting these functions. Within the human tooth and its surrounding tissues, different stem cell populations can be distinguished, such as dental pulp stem cells, stem cells from human deciduous teeth, stem cells from the apical papilla, dental follicle stem cells, and periodontal ligament stem cells. Given their straightforward and relatively easy isolation from extracted third molars, dental stem cells (DSCs) have become an attractive source of mesenchymal-like stem cells. Over the past decade, there have been numerous studies supporting the angiogenic, neuroprotective, and neurotrophic effects of the DSC secretome. Together with their ability to differentiate into endothelial cells and neural cell types, this makes DSCs suitable candidates for dental tissue engineering and nerve injury repair.

scholarly journals Tooth Bioengineering and Regenerative Dentistry

2019 ◽  
Vol 98 (11) ◽  
pp. 1173-1182 ◽  
Author(s):  
P.C. Yelick ◽  
P.T. Sharpe
Keyword(s):  
Tissue Engineering ◽  
Current Knowledge ◽  
Basic Research ◽  
Dental Tissue ◽  
Dental Tissues ◽  
The Past ◽  
Tremendous Progress ◽  
Regenerative Dentistry ◽  
Dental Tissue Engineering ◽  
Made In

Over the past 100 y, tremendous progress has been made in the fields of dental tissue engineering and regenerative dental medicine, collectively known as translational dentistry. Translational dentistry has benefited from the more mature field of tissue engineering and regenerative medicine (TERM), established on the belief that biocompatible scaffolds, cells, and growth factors could be used to create functional, living replacement tissues and organs. TERM, created and pioneered by an interdisciplinary group of clinicians, biomedical engineers, and basic research scientists, works to create bioengineered replacement tissues that provide at least enough function for patients to survive until donor organs are available and, at best, fully functional replacement organs. Ultimately, the goal of both TERM and regenerative dentistry is to bring new and more effective therapies to the clinic to treat those in need. Very recently, the National Institutes of Health/National Institute of Dental and Craniofacial Research invested $24 million over a 3-y period to create dental oral and craniofacial translational resource centers to facilitate the development of more effective therapies to treat edentulism and other dental-related diseases over the next decade. This exciting era in regenerative dentistry, particularly for whole-tooth tissue engineering, builds on many key successes over the past 100 y that have contributed toward our current knowledge and understanding of signaling pathways directing natural tooth and dental tissue development—the foundation for current strategies to engineer functional, living replacement dental tissues and whole teeth. Here we use a historical perspective to present key findings and pivotal advances made in the field of translational dentistry over the past 100 y. We will first describe how this process has evolved over the past 100 y and then hypothesize on what to expect over the next century.

Dental tissue engineering: Future of regenerative dentistry

2016 ◽  
Vol 3 (1) ◽  
pp. 31 ◽  
Author(s):  
Rohan Shah ◽  
Darshan Hiremutt ◽  
Shweta Jajoo ◽  
Amol Kamble
Keyword(s):  
Tissue Engineering ◽  
Dental Tissue ◽  
Regenerative Dentistry ◽  
Dental Tissue Engineering

Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

2011 ◽  
Vol 11 ◽  
pp. 13-20 ◽  
Author(s):  
Tran Le Bao Ha ◽  
Doan Nguyen Vu ◽  
To Minh Quan ◽  
Ngoc Phan Kim ◽  
Hung Hoang Tu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Dental Tissue ◽  
Dentin Surface ◽  
Human Dental Pulp ◽  
Dental Tissue Engineering ◽  
High Level

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

scholarly journals Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

2016 ◽  
Vol 3 (4) ◽  
pp. 263-276 ◽  
Author(s):  
Fugui Zhang ◽  
Jinlin Song ◽  
Hongmei Zhang ◽  
Enyi Huang ◽  
Dongzhe Song ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bmp Signaling ◽  
Dental Stem Cells ◽  
Dental Tissue ◽  
Signaling Crosstalk ◽  
Dental Tissue Engineering
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