Green Synthesis of Silver Nanoparticles Using Waste Tea Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 1-14
Author(s):  
Darshana Rajput ◽  
Samrat Paul ◽  
Annika Gupta

Green synthesis of silver nanoparticles has gained momentum since the demand to synthesize nanoparticles in an eco-friendly way has increased significantly. Here we report, economic and cost-effective biosynthesis of silver nanoparticles using waste of tea leaves (Camelia sinesis). The aim of the study was to biosynthesize silver nanoparticles and to assess its potential applications such as antibacterial activity, plant growth induction and dye degradation. Standardization studies were done using UV- Spectroscopy to determine the optimum synthesis condition for synthesis of silver nanoparticles. The optimum conditions were found to be pH 6.0, ambient temperature condition and 5mM AgNO3 concentration. Characterization studies using UV-Visible Spectroscopy, TEM and AFM analysis show nanoscale range of the particles. The silver nanoparticles showed maximum antibacterial activity against K. pneumonia followed by E. coli and minimum activity against C. diptheriae. The nanoparticles showed significant effect on the growth of Vigna radiata seeds at 50% concentration of nanoparticles. The particles immobilized on cotton cloth showed antibacterial activity against Gram positive organisms. Dye degradation studies showed that the nanoparticles are able to degrade phenol red and blue textile dye effectively.

Author(s):  
Wilson Mbiti Njue ◽  
Jackson Kilonzo Kithokoi ◽  
Jane Mburu ◽  
Henry Mwangi ◽  
Sauda Swaleh

Metal nanoparticles in the field of nanotechnology are of great interest to modern scientific research due to their size effects, medical uses and, catalytic, electronic and optical properties. Green synthesis of metal nanoparticles is a feasible alternative to chemical methods as it is environmentally friendly and cost effective. In continuation with our research on green synthesis of silver nanoparticles using Kenyan medicinal plants, we here report the synthesis of novel silver nanoparticles (AgNPs) on ultrasonic bath using Adansonia digitata leaves extracts and analysis of their antibacterial activity. The nanoparticles were characterized by UV-Vis, High Resolution Transmission Electron Microscopy (HRTEM), FTIR spectroscopy and Energy Dispersive X-ray (EDX). EDX analysis affirmed the nanoparticles were pure silver. Crystalline nature of the nanoparticles was confirmed by bright circular spots in the Selected Area Electron Diffraction (SAED) in HRTEM image. The AgNPs were spherical with an average size 13 nm. FTIR analysis showed strong –C=C- and –OH stretching bands due to compounds capping the nanoparticles. The synthesized AgNPs showed high inhibition zones of 17.1±0.130 mm towards Gram-negative bacteria E. coli and 12.9±0.082mm towards Gram positive bacteria S. aureus. The aqueous A. digitata extract had no effect on growth inhibition of test bacteria. The study showed that the silver nanoparticles synthesized from the plant’s leaves extract had antibacterial activity against both Gram negative and positive pathogenic bacteria. The nanoparticles can be utilized towards developing novel drugs useful in combating pathogens.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.


2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).


Author(s):  
S J Mane Gavade ◽  
G H Nikam ◽  
R S Dhabbe ◽  
S R Sabale ◽  
B V Tamhankar ◽  
...  

2015 ◽  
Vol 33 (5) ◽  
pp. 445-455 ◽  
Author(s):  
Kiran Jadhav ◽  
Dinesh Dhamecha ◽  
Bhagyashree Dalvi ◽  
Mrityunjaya Patil

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