Carboxyl-modified single-wall carbon nanotubes improve bone tissue formation in vitro and repair in an in vivo rat model

Silica aerogels have attracted increasingly more attention due to their extraordinary properties and their existing and potential applications in a wide variety of technological areas. Materials that promote bone-tissue formation at their surface and bond to osseous tissues when implanted are called bioactive, such as pseudowollastonite particles. In this work, the synthesis of aerogels with pseudowollastonite particles was performed. The synthesis involved the preparation of an alcogel by a two step sol-gel route followed by ambient pressure drying. To promote a higher bioactivity the obtained aerogels were then biomimetically treated using simulated body fluids, SBF and 1.5 SBF. A high bioactivity was demonstrated by FT-IR, SEM, EDS, and XRD. The in vitro biocompatibility was assessed by testing cytotoxicity using rat osteoblasts cultures. The results obtained indicate that these materials are highly potential aerogels for bone tissue regeneration.

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Human bone tissue formation in diffusion chamber culture in vivo by bone-derived cells and marrow stromal fibroblastic cells

Bone ◽

10.1016/8756-3282(95)00112-q ◽

1995 ◽

Vol 16 (6) ◽

pp. 597-601 ◽

Cited By ~ 129

Author(s):

R. Gundle ◽

C.J. Joyner ◽

J.T. Triffitt

Keyword(s):

Bone Tissue ◽

Diffusion Chamber ◽

Human Bone ◽

Tissue Formation ◽

Human Bone Tissue ◽

Diffusion Chamber Culture ◽

Bone Tissue Formation ◽

Fibroblastic Cells

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The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation

Biomedical Materials ◽

10.1088/1748-605x/aa9717 ◽

2018 ◽

Vol 13 (2) ◽

pp. 025004 ◽

Cited By ~ 9

Author(s):

M Rampichová ◽

J Chvojka ◽

V Jenčová ◽

T Kubíková ◽

Z Tonar ◽

...

Keyword(s):

Bone Tissue ◽

Cell Infiltration ◽

Tissue Formation ◽

Bone Tissue Formation

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Creation of Three-dimensional Composites of Tricalcium Phosphate and Single-wall Carbon Nanotubes for Bone Tissue Engineering

2021 IEEE Conference of Russian Young Researchers in Electrical and Electronic Engineering (ElConRus) ◽

10.1109/elconrus51938.2021.9396648 ◽

2021 ◽

Author(s):

Svetlana Z. Zhovnir ◽

Artem V. Kuksin ◽

Mikhail S. Saveliev ◽

Ivan V. Pyanov ◽

Denis T. Murashko

Keyword(s):

Carbon Nanotubes ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tricalcium Phosphate ◽

Three Dimensional ◽

Single Wall Carbon Nanotubes ◽

Single Wall Carbon

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Conductive single-wall carbon nanotubes/extracellular matrix hybrid hydrogels promote the lineage-specific development of seeding cells for tissue repair through reconstructing an integrin-dependent niche

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00993-3 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Rui Bai ◽

Jianfeng Liu ◽

Jiao Zhang ◽

Jinmiao Shi ◽

Zhigeng Jin ◽

...

Keyword(s):

Carbon Nanotubes ◽

Stem Cells ◽

Extracellular Matrix ◽

Tissue Repair ◽

Single Wall Carbon Nanotubes ◽

Hybrid Hydrogel ◽

Hybrid Hydrogels ◽

Biophysical Cues

Abstract Background The niche of tissue development in vivo involves the growth matrix, biophysical cues and cell-cell interactions. Although natural extracellular matrixes may provide good supporting for seeding cells in vitro, it is evitable to destroy biophysical cues during decellularization. Reconstructing the bioactivities of extracellular matrix-based scaffolds is essential for their usage in tissue repair. Results In the study, a hybrid hydrogel was developed by incorporating single-wall carbon nanotubes (SWCNTs) into heart-derived extracellular matrixes. Interestingly, insoluble SWCNTs were well dispersed in hybrid hydrogel solution via the interaction with extracellular matrix proteins. Importantly, an augmented integrin-dependent niche was reconstructed in the hybrid hydrogel, which could work like biophysical cues to activate integrin-related pathway of seeding cells. As supporting scaffolds in vitro, the hybrid hydrogels were observed to significantly promote seeding cell adhesion, differentiation, as well as structural and functional development towards mature cardiac tissues. As injectable carrier scaffolds in vivo, the hybrid hydrogels were then used to delivery stem cells for myocardial repair in rats. Similarly, significantly enhanced cardiac differentiation and maturation(12.5 ± 2.3% VS 32.8 ± 5%) of stem cells were detected in vivo, resulting in improved myocardial regeneration and repair. Conclusions The study represented a simple and powerful approach for exploring bioactive scaffold to promote stem cell-based tissue repair. Graphic abstract

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Evaluation of the Skin Sensitization Potential of Carbon Nanotubes Using Alternative In Vitro and In Vivo Assays

Toxics ◽

10.3390/toxics8040122 ◽

2020 ◽

Vol 8 (4) ◽

pp. 122

Author(s):

Sung-Hyun Kim ◽

Dong Han Lee ◽

Jin Hee Lee ◽

Jun-Young Yang ◽

Hyo-Sook Shin ◽

...

Keyword(s):

Carbon Nanotubes ◽

Large Scale ◽

Test Method ◽

Single Wall Carbon Nanotubes ◽

Scale Production ◽

Skin Sensitization ◽

Alternative Test ◽

Alternative Test Method

Carbon nanotubes (CNTs) are one of the major types of nanomaterials that have various industrial and biomedical applications. However, there is a risk of accidental exposure to CNTs in individuals involved in their large-scale production and in individuals who use products containing CNTs. This study aimed to evaluate the skin sensitization induced by CNTs using two alternative tests. We selected single-wall carbon nanotubes and multi-walled carbon nanotubes for this study. First, the physiochemical properties of the CNTs were measured, including the morphology, size, and zeta potential, under various conditions. Thereafter, we assessed the sensitization potential of the CNTs using the ARE-Nrf2 Luciferase KeratinoSens™ assay, an in vitro alternative test method. In addition, the CNTs were evaluated for their skin sensitization potential using the LLNA: BrdU-FCM in vivo alternative test method. In this study, we report for the first time the sensitization results of CNTs using the KeratinoSens™ and LLNA: BrdU-FCM test methods in this study. This study found that both CNTs do not induce skin sensitization. These results suggest that the KeratinoSens™ and LLNA: BrdU-FCM assay may be useful as alternative assays for evaluating the potential of some nanomaterials that can induce skin sensitization.

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