scholarly journals Long Non-Coding RNA SNHG7 Alleviates Oxygen and Glucose Deprivation/Reoxygenation-Induced Neuronal Injury by Modulating miR-9/SIRT1 Axis in PC12 Cells: Potential Role in Ischemic Stroke

2020 ◽  
Vol Volume 16 ◽  
pp. 2837-2848
Author(s):  
Tao Zhou ◽  
Shuai Wang ◽  
Kai Lu ◽  
Chunhui Yin
Keyword(s):  
Ischemic Stroke ◽  
Pc12 Cells ◽  
Potential Role ◽  
Neuronal Injury ◽  
Glucose Deprivation ◽  
Oxygen And Glucose Deprivation ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Long non-coding RNA MEG3 alters neuronal apoptosis and is associated with poor prognosis in ischemic stroke: based on basic and prospective studies

2019 ◽  
Author(s):  
Meiping Wang ◽  
Wenjuan Chen ◽  
Yu Geng ◽  
Chenghua Xu ◽  
Xiaoxiao Tao ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Poor Prognosis ◽  
Infarct Volume ◽  
High Sensitivity ◽  
Glucose Deprivation ◽  
Expression Level ◽  
Control Group ◽  
Blood Leukocytes ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background: Ischemic stroke (IS) is a highly heterogeneous disease with variable pathogenesis. Due to the lack of early predictive markers, the mortality rate of IS remains high worldwide. This study focused on the expression pattern of long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) and its prognostic value in IS. Methods: Twenty-four ICR mice (30-35g, 12 males and 12 females) were involved in this study. Middle cerebral artery occlusion (MCAO) was established to simulate an IS environment. Oxygen-glucose deprivation/reoxygenation (OGD/R) in cells were also performed. A total of 215 IS patients and 153 age and gender-matched controls were included. Quantitative Real-time PCR (qRT-PCR) was performed to detect the expression of MEG3 in peripheral blood leukocytes. Results: The MEG3 expression in MCAO group was significantly higher than control group (P=0.004), and the survival time of high MEG3 group was significantly lower than that of the low MEG3 group (P=0.042). OGD/R promotes cell apoptosis, while si-MEG3 inhibits apoptosis. Results of WB showed that, expression level of Bax was down regulated by si-MEG3, while OGD/R increased its expression level. MEG3 was significantly up-regulated in IS patients and associated with the National Institutes of Health Stroke Scale (NIHSS) (r=0.347, P<0.001), modified Rankin Scale (mRS) (r=0.385, P<0.001), high sensitivity C-reactive protein (Hs-CRP) (r=0.221, P=0.002) level and infarct volume (r=0.201, P=0.006). Overall survival analysis showed that patients with higher MEG3 expression had a relatively poor prognosis (P<0.001). Meanwhile, multivariate analysis revealed that MEG3 was an independent prognostic marker of functional outcome and death in patients with IS. Conclusions: Current study suggested that MEG3 might be considered as a potential prognostic indicator in IS.

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