scholarly journals Lack of correlation between in silico projection of function and quantitative real-time PCR-determined gene expression levels in colon tissue

2013 ◽  
pp. 99
Author(s):  
Susan Kadlubar ◽  
Rosalind Penney ◽  
Abbie Lundgreen ◽  
Aiwei Yao-Borengassar ◽  
Vineetha Koroth-Edavana ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
In Silico ◽  
Quantitative Real Time Pcr ◽  
Colon Tissue ◽  
Expression Levels ◽  
Gene Expression Levels

Discrimination and evaluation of lactoferrin and delta-lactoferrin gene expression levels in cancer cells and under inflammatory stimuli using TaqMan real-time PCR

BioMetals ◽  
2010 ◽  
Vol 23 (3) ◽  
pp. 441-452 ◽  
Author(s):  
Esthelle Hoedt ◽  
Stephan Hardivillé ◽  
Christophe Mariller ◽  
E. Elass ◽  
Jean-Paul Perraudin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cancer Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Expression Levels ◽  
Inflammatory Stimuli ◽  
Lactoferrin Gene ◽  
Gene Expression Levels

scholarly journals Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber–pumpkin grafted plants

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6536 ◽  
Author(s):  
Li Miao ◽  
Xing Qin ◽  
Lihong Gao ◽  
Qing Li ◽  
Shuzhen Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Gene ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Stable Expression ◽  
Quantitative Real Time Pcr ◽  
Graft Union ◽  
Expression Levels ◽  
Qrt Pcr

Background Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput technique to measure mRNA transcript levels. The accuracy of this evaluation of gene expression depends on the use of optimal reference genes. Cucumber–pumpkin grafted plants, made by grafting a cucumber scion onto pumpkin rootstock, are superior to either parent plant, as grafting conveys many advantages. However, although many reliable reference genes have been identified in both cucumber and pumpkin, none have been obtained for cucumber–pumpkin grafted plants. Methods In this work, 12 candidate reference genes, including eight traditional genes and four novel genes identified from our transcriptome data, were selected to assess their expression stability. Their expression levels in 25 samples, including three cucumber and three pumpkin samples from different organs, and 19 cucumber–pumpkin grafted samples from different organs, conditions, and varieties, were analyzed by qRT-PCR, and the stability of their expression was assessed by the comparative ΔCt method, geNorm, NormFinder, BestKeeper, and RefFinder. Results The results showed that the most suitable reference gene varied dependent on the organs, conditions, and varieties. CACS and 40SRPS8 were the most stable reference genes for all samples in our research. TIP41 and CACS showed the most stable expression in different cucumber organs, TIP41 and PP2A were the optimal reference genes in pumpkin organs, and CACS and 40SRPS8 were the most stable genes in all grafted cucumber samples. However, the optimal reference gene varied under different conditions. CACS and 40SRPS8 were the best combination of genes in different organs of cucumber–pumpkin grafted plants, TUA and RPL36Aa were the most stable in the graft union under cold stress, LEA26 and ARF showed the most stable expression in the graft union during the healing process, and TIP41 and PP2A were the most stable across different varieties of cucumber–pumpkin grafted plants. The use of LEA26, ARF and LEA26+ARF as reference genes were further verified by analyzing the expression levels of csaCYCD3;1, csaRUL, cmoRUL, and cmoPIN in the graft union at different time points after grafting. Discussion This work is the first report of appropriate reference genes in grafted cucumber plants and provides useful information for the study of gene expression and molecular mechanisms in cucumber–pumpkin grafted plants.

scholarly journals Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56296 ◽  
Author(s):  
Yoshiki Fujii ◽  
Kazutaka Kitaura ◽  
Takaji Matsutani ◽  
Kenji Shirai ◽  
Satsuki Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Related Gene ◽  
Quantitative Real Time Pcr ◽  
Common Marmosets ◽  
Immune Related Gene
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