The influence of cultivation conditions on the level of ploidy of regenerated plants of Brassica oleracea L. obtained from the reproductive organs in vitro

2017 ◽  
Vol 1 (67) ◽  
pp. 115-119
Author(s):  
Rima Kirakosyan ◽  
◽  
Elena Kalashnikova ◽  
Keyword(s):  
Brassica Oleracea ◽  
Reproductive Organs ◽  
Cultivation Conditions ◽  
Regenerated Plants ◽  
Brassica Oleracea L

scholarly journals Cytological methods of analysis of haploid plants-regenerants of cabbage (Brassica oleracea L.) obtained in vitro

2019 ◽  
pp. 13-15
Author(s):  
Rima N. Kirakosyan ◽  
Elena A. Kalashnikovа
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploids ◽  
Reproductive Organs ◽  
Universal Method ◽  
Mineral Salts ◽  
Methods Of Analysis ◽  
White Cabbage ◽  
Regenerated Plants ◽  
Haploid Plants

Relevance Currently, in genetic studies and selection of cabbage cultures, biotechnological methods for creating clean lines — doubled haploids in the culture of anthers and in the culture of isolated microspores are widely used. A common feature of these technologies is that the plants obtained in vitro have different levels of ploidy and along with doubled haploids there are haploid, tetraploid and mixoploid forms. Therefore, the use of new cytological methods of analysis of haploid plants remains an urgent problem. Material and method The aim of this work is to establish the genetic nature of regenerated plants of Brassica oleracea L., obtained from reproductive organs in vitro. Isolated anthers and ovaries of white cabbage were cultivated on solid nutrient media containing mineral salts according to the recipe Murashige and Skoog (MS). The obtained regenerated plants were used to calculate the number of chromosomes in the root meristem, as well as the number of chloroplasts in the cells of the closing stomata of leaves using the new universal method of preparing preparations of plant chromosomes – “SteamDrop”. Results As a result of the research, the dependence of the level of ploidy on the cultivation conditions was studied. It has been shown that plants-regenerants of white cabbage, obtained in vitro from reproductive organs, had a different set of chromosomes (n, 3n, 5n). It was established that the number of chloroplasts in the stomatal cells of regenerated plants was from 9 to 45, while the original donor plants had 18–20.

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals PENGARUH EKSTRAK KUBIS (Brassica oleracea L. var. capitata L.) DALAM PEMBENTUKAN ZONA HAMBAT TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans PADA KARIES (In Vitro)

2019 ◽  
Vol 3 (2) ◽  
pp. 90-96
Author(s):  
Nisaummahmudah Nisaummahmudah ◽  
Kornialia Kornialia ◽  
Nurhamidah Nurhamidah
Keyword(s):  
Brassica Oleracea ◽  
Streptococcus Mutans ◽  
P Value ◽  
Disc Diffusion ◽  
Brassica Oleracea L

Gigi dan mulut merupakan investasi utama bagi kesehatan. Apabila kesehatan gigi dan mulut sering diabaikan, maka akan menimbulkan masalah pada gigi dan mulut maupun kesehatan secara umum. Masalah kesehatan gigi dan mulut yang banyak terjadi di masyarakat adalah karies gigi. Streptococcus mutans merupakan salah satu mikroorganisme spesifik penyebab karies gigi. Pencegahan karies dapat dilakukan dengan penggunaan bahan antimikroba yang berasal dari alam seperti kubis. Kubis (Brassica oleracea L.var capitata L.) merupakan salah satu hasil bumi Indonesia yang memiliki sifat antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kubis dalam pembentukan zona hambat terhadap pertumbuhan bakteri Streptococcus mutans pada karies (invitro). Metode penelitian yang digunakan adalah eksperimental laboratorium. Penelitian dilakukan di Laboratorium Kimia Kopertis wilayah X Padang, Sumatera Barat. Konsentrasi ekstrak kubis yang digunakan adalah 15%, 20%, 25% dan 30%. Pengujian antibakteri dilakukan dengan metode disc diffusion dengan mengukur zona hambat yang mengindikasikan adanya hambatan pertumbuhan bakteri Streptococcus mutans oleh ekstrak kubis. Hasil penelitian diuji menggunakan uji Anova, diketahui rerata diameter zona hambat paling besar adalah pada konsentrasi 30% (16,69 mm). Nilai p-value dari hasil tes uji Anova p-value

scholarly journals Embedding in Filter-Sterilized Alginate Enhances Brassica Oleracea L. Protoplast Culture

2015 ◽  
Vol 56 (2) ◽  
pp. 20-26
Author(s):  
Agnieszka Kiełkowska ◽  
Adela Adamus
Keyword(s):  
Brassica Oleracea ◽  
Sodium Alginate ◽  
Mitotic Activity ◽  
Protoplast Culture ◽  
Division Frequency ◽  
Hypocotyl Tissue ◽  
Brassica Oleracea L

Abstract The influence of sodium alginate sterilization on the viability and mitotic activity of embedded protoplasts was studied in protoplasts of Brassica oleracea subsp. alba and rubra isolated from hypocotyl tissue and leaves of seedlings or plants grown in vitro. Both leaf and hypocotyl-derived protoplasts were more viable and divided more frequently when embedded in filtrated alginate. Division frequency was highest in cv. Reball F1 and the mitotic activity of its protoplasts was three times higher when embedded in filtrated alginate (36.1 ± 6.8%) than when cultured in autoclaved alginate (10.9 ± 5.0%). Protoplast-derived calli colonies were transferred to solid regeneration media and plants of all tested accessions were obtained.

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