Cystic hygroma of the neck as an alternative sourse of cells for prenatal karyotyping

2018 ◽  
Author(s):  
L.A. Êhlevnaya, S.A. Malova, N.V. Mazurik, N.Z. Malykhina
Keyword(s):  
Cell Cultures ◽  
Standard Method ◽  
Cystic Hygroma ◽  
Monolayer Cell ◽  
Cultivation Period

The results of studies about the possibility of using liquid from the fetal cystic hygroma of the neck for prenatal karyotyping are presented. Aspiration of fetal cystic formations was carry out by five women. A standard method for producing monolayer cell cultures with their own modifications was used for the work. In all cases, the chromosomal pathology of the fetus was confirmed 45,X. There were noted the specific features of the cultivation of various fluid's samples. The reasons that could influence on the change in the cultivation period were analyzed.

An improved approach to freeze-fracture morphology of monolayer cell cultures

1998 ◽  
Vol 82 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Elisabeth Bugnard ◽  
Patrick Sors ◽  
Alain Bloc ◽  
Françoise Loctin ◽  
Yves Dunant
Keyword(s):  
Cell Cultures ◽  
Freeze Fracture ◽  
Fracture Morphology ◽  
Monolayer Cell

Studies on the renin-angiotensin system in primary monolayer cell cultures of the rat epididymis

1991 ◽  
Vol 131 (2) ◽  
pp. 287-293 ◽  
Author(s):  
P. Y. D. Wong ◽  
C. N. Uchendu
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Culture Medium ◽  
Renin Angiotensin System ◽  
Cell Culture Medium ◽  
Angiotensin System ◽  
Monolayer Cell ◽  
Renin Angiotensin ◽  
Cell Monolayers ◽  
Cell Lysate

ABSTRACT Monolayer cell cultures formed from the rat cauda epididymidis exhibited renin-like and angiotensin-converting enzyme (ACE) activities and contained immunoreactive angiotensin I (AI) and angiotensin II (AII). Renin-like activity, determined indirectly by radioimmunoassay of generated AI at a near-neutral pH of 6·0, was demonstrated in the cell lysate but was almost undetectable in the serum-free cell culture medium, suggesting that renin expression in epididymal cells is an intracellular phenomenon. In contrast, both AI and AII were detected in the cell lysate and cell culture medium. The level of AI was enhanced by pretreating the cells with the ACE inhibitor captopril (100 nmol/l). Incubating the cell monolayers with thoroughly washed sperm cells obtained from the intact cauda epididymides of rats increase (P < 0·01) the AII content of the cell culture medium, with a parallel decline (P < 0·01) in the AI concentration. However, adrenaline (0·23 μmol/l), which was found to stimulate electrogenic anion secretion by cell monolayers grown on pervious supports, was without effect on the renin-like activity or concentration of angiotensins. The ACE activity in cells was confirmed by its strong dependence on chloride ion and its susceptibility to inhibition by captopril (100 nmol/l). Enzyme activity was significantly (P < 0·005) higher in the culture medium than in the cell lysate and cell membrane fragments. Angiotensinogen, which is obligatory for an intrinsic renin-angiotensin system, is present in epididymal cells. Presumably, it is synthesized and processed in the cell cytosol by intracellular renin. These findings in cultured cells provide further evidence for a local renin–angiotensin system in epididymal tissue and suggest a possible role of endogenous AII in the regulation of epididymal function. Journal of Endocrinology (1991) 131, 287–293

Short??Term Effects of Radiographic Contrast Media on Monolayer Cell Cultures and Hepatocytes

1987 ◽  
Vol 22 (7) ◽  
pp. 603-607 ◽  
Author(s):  
ASBJ??RN NORDBY ◽  
JOSTEIN HALGUNSET ◽  
KETIL THORSTENSEN ◽  
OLAV A. HAUGEN
Keyword(s):  
Contrast Media ◽  
Cell Cultures ◽  
Short Term ◽  
Monolayer Cell ◽  
Radiographic Contrast ◽  
Radiographic Contrast Media ◽  
Short Term Effects

Effects of millimeter-wave radiation on monolayer cell cultures. III. A search for frequency-specific athermal biological effects on protein synthesis

1981 ◽  
Vol 2 (2) ◽  
pp. 151-159 ◽  
Author(s):  
L. G. Bush ◽  
D. W. Hill ◽  
A. Riazi ◽  
L. J. Stensaas ◽  
L. M. Partlow ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Millimeter Wave ◽  
Cell Cultures ◽  
Biological Effects ◽  
Wave Radiation ◽  
Monolayer Cell
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