PPARγ gene expression analysis in psoriasis treatment

Introduction. PPARγ is the most studied PPAR subtype and is expressed predominantly in adipose tissue, heart, colon, kidney, spleen, intestine, skeletal muscle, liver, macrophages, and skin. In the skin, PPARγ controls the genetic regulation of gene network expression involved in cell proliferation, differentiation, and inflammatory responses. PPARγ (Peroxisome proliferator-activated receptor gamma) has only recently come to be considered a key player in the development and pathogenesis of psoriasis and psoriatic inflammatory conditions.Aim of the study. To study PPARγ gene expression in the affected skin of psoriasis patients in comparison with visually unaffected skin. To study changes in PPARγ gene expression level in psoriasis affected skin in comparison with unaffected skin in patients before and after treatment with low-level laser radiation with a wavelength of 1.27 μm.Materials and methods. Twelve patients with psoriasis participated in the study. Biopsies from unaffected skin areas were taken at a distance of about 3 cm from the affected skin. Analysis was performed by real-time PCR.Results and Discussion. We quantitatively measured PPARγ gene expression using RT-PCR in the affected skin of patients with psoriasis in comparison with visually unaffected skin in the same patients before and after treatment with low-level laser radiation with a wavelength of 1.27 μm (the short-wave part of the infrared range). The study experimentally showed a 1.3 ± 0.27-fold decrease in PPARγ gene expression in the affected skin of psoriasis patients on average. Significant increase in over-expression of PPARγ gene up to 2,13 ± 0,47 times was observed after treatment of patients with low-level laser radiation.Conclusions. PPARγ gene expression may be an indicator of the efficacy of psoriasis treatment at the molecular level, as well as become a new therapeutic target.

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Alteration of STAT3 gene expression in psoriasis treatment

Medical Council ◽

10.21518/2079-701x-2020-12-71-74 ◽

2020 ◽

pp. 71-74

Author(s):

V. V. Sobolev ◽

E. V. Denisova ◽

I. M. Korsunskaya

Keyword(s):

Gene Expression ◽

Laser Radiation ◽

Short Wave ◽

Pcr Analysis ◽

Low Level ◽

Psoriasis Treatment ◽

Low Level Laser ◽

Inflammatory Conditions ◽

Level Laser ◽

Before And After

Introduction. Psoriasis is a typical complex multigenic and multifactorial disease with heterogeneous genetic heredity, which requires the interaction of genes both with each other and with environmental factors. STAT3 (Signal Transducer and Activator of Transcription 3) has only recently been considered a key player in the development and pathogenesis of psoriasis and psoriatic inflammatory conditions.Aim of the study. To study the expression of the STAT3 gene in the affected part of the skin of psoriasis patients in relation to the visually unaffected part. To study the change in the STAT3 gene expression level in psoriasis-affected skin as compared to nonaffected skin in patients before and after treatment with low-level laser radiation at a wavelength of 1.27 pm.Materials and methods. The study involved 12 psoriasis patients. Biopsies from the unaffected skin were taken at a distance of about 3 cm from the affected skin. Real-time PCR analysis was performed.Results and discussion. The expression of the STAT3 gene was quantitatively measured using RT-PCR in the affected part of the skin of psoriasis patients compared to the visually unaffected part of the skin of the same patients before and after treatment with low-level laser radiation with a wavelength of 1.27 gm (short-wave infrared). As a result of the study, an increase in the expression of the STAT3 gene in the affected part of the skin of psoriasis patients of an average of 3.96 ± 2 times was experimentally shown. A decrease in gene expression was observed in psoriasis affected skin compared to samples of non-affected areas. After treatment of patients with low-level laser radiation, a significant reduction in the expression of the overexpressed STAT3 gene to 1.75 ± 0.5 times was observed.Conclusions. The transcription activity of the STAT3 gene can be an indicator of the efficacy of psoriasis treatment at the molecular level and can also be a new therapeutic target.

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Low-Level Laser Therapy Techniques and Equipment for Occupational Disease Prevention in Precision Workers

ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT ◽

10.35627/2219-5238/2021-29-9-78-83 ◽

2021 ◽

pp. 78-83

Author(s):

NYu Mal’kova ◽

MD Petrova

Keyword(s):

Spinal Cord ◽

Laser Radiation ◽

Laser Therapy ◽

Blood Circulation ◽

Low Level Laser Therapy ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Before And After ◽

Spinal Cord Disorders

Background: The problem of protecting and maintaining health of workers in the microelectronics industry exposed to adverse occupational factors including tension and overstrain of the muscles of the upper extremities and visual strain, gains special relevance. Objective: Introduction of the developed and patented low-level laser therapy techniques to recover functions of vision and the musculoskeletal system impaired in the course of work. Materials and methods: The study involved 65 chip assemblers aged 26–41 years. Their eyes, the back of the hands, and the collar area were exposed to low-level laser radiation from the red region of the spectrum. All employees were examined by a neurologist and a surgeon and their working conditions were evaluated. The accommodation apparatus was tested using an AKA-01 type accommodation meter. Results: After five 10-minute sessions, we registered an increase in the volume of absolute accommodation during the observation period before and after the work shift by 28 % and 19 %, respectively. On subsequent days of observation, we noted an increase in the accommodation during 10 days, followed by a decrease by the 30th day of observation. Application of preventive techniques helped eliminate spinal cord disorders in all workers. Blood circulation in fingers increased by 66 % compared to the initial condition. Discussion: Low-level laser therapy improves blood supply, intensifies redox processes in the retina of the eye, skin cells, and blood vessels. Conclusions: Low-level laser radiation of the red region of the spectrum relieves the spasm of accommodation and normalizes the efficiency of the accommodation apparatus. Such laser therapy helps eliminate spinal cord disorders and improve blood circulation. Introduction of the developed techniques can prevent occupational diseases and reduce disability in patients.

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Clinical Efficacy of Fluoride Varnish and Low-Level Laser Radiation in Treating Dentin Hypersensitivity

Brazilian Dental Journal ◽

10.1590/0103-6440201602422 ◽

2016 ◽

Vol 27 (1) ◽

pp. 79-82 ◽

Cited By ~ 10

Author(s):

Euler Maciel Dantas ◽

Fernanda Kyarelly de Oliveira Amorim ◽

Fernando José de Oliveira Nóbrega ◽

Poliana Medeiros Cunha Dantas ◽

Rodrigo Gadelha Vasconcelos ◽

...

Keyword(s):

Laser Radiation ◽

Adult Population ◽

Fluoride Varnish ◽

Cervical Region ◽

Dentin Hypersensitivity ◽

Short Term Treatment ◽

Painful Condition ◽

Low Level ◽

Low Level Laser ◽

Level Laser

Abstract The objective of this study was to compare the efficacy of fluoride varnish (Fluorniz(r)) and irradiation with a gallium-arsenide-aluminum diode laser in the treatment of cervical dentin hypersensitivity. Cervical dentin hypersensitivity (CDH) is a painful condition that is highly prevalent in the world's adult population, with one in six patients presenting this symptom. Eighty-six teeth were divided into two groups: Group 1, teeth treated with Fluorniz; Group 2, teeth irradiated with a GaAlAs laser at a 4 J/cm2 dose. The two treatments were applied to the buccal cervical region in four sessions, at intervals of 72 to 96 h. The response of the patient to tactile and thermal-evaporative stimuli was rated on a visual analog scale. The results showed a reduction of hypersensitivity in response to tactile and thermal-evaporative stimulation at the end of treatment in both groups. In conclusion, short-term treatment with Fluorniz was found to be more effective than low-level laser radiation in reducing cervical dentin hypersensitivity.

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