scholarly journals Immunohistochemical localization of adropin in the small intestine of rats

2019 ◽  
Vol 75 (05) ◽  
pp. 6246-2019
Author(s):  
MARTA PAWŁOWSKA-OLSZEWSKA ◽  
IWONA PUZIO ◽  
MAŁGORZATA KAPICA ◽  
GRZEGORZ TYMICKI. ◽  
WIESŁAW ŚLEBODA ◽  
...  
Keyword(s):  
Small Intestine ◽  
Wistar Rats ◽  
Immunohistochemical Localization ◽  
Middle Part ◽  
Sham Operation ◽  
Submucosal Plexus ◽  
Immune Reactions ◽  
Immunofluorescence Studies ◽  
Potential Impact ◽  
Muscularis Externa

The aim of this study was to determine the occurrence and distribution of adropin in the small intestine in control and gastrectomized rats. The study was carried out on 12 Wistar rats. Six control rats (SHO) underwent a sham operation. Six rats were subjected to gastrectomy (Gx); i.e. resection of the antrum and the glandular part of the stomach. Six weeks after the surgery, the rats were sacrificed. The duodenum and middle part of the jejunum were collected for immunohistochemical and immunofluorescence procedures. An immunopositive reaction to ADR was detected in the duodenum and jejunum, in crypts, and in enterocytes located along the entire length of the villi. Furthermore, more intense ADR immunoreactivity was observed in the crypts and villi of the duodenum than in the jejunum. The presence of adropin was also detected in the submucosa and muscularis externa of the duodenum and jejunum, in the cells of Brunner’s gland of the duodenum, and in myenteric and submucosal plexus nerve. The ADR immunoreactivity in the crypts and villi in the duodenum and jejunum was lower in the Gx rats compared to the SHO rats. The ADR distribution in the duodenum and jejunum has also been confirmed in immunofluorescence studies. In conclusion, our data demonstrated ADR expression in all histological layers of the small intestine in the rats. Gx reduced ADR immunoreactivity in the crypts and enterocytes. This may suggest a potential impact of ADR on intestine function such as absorbability and immune reactions. However, further research is needed to explain the ADR role in gastrointestinal functions

A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

2001 ◽  
Vol 16 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Gracinda De Lourdes Jorge ◽  
Luiz Sergio Leonardi ◽  
Ilka de Fatima Santana Ferreira Boin ◽  
Orlando de Castro e Silva Jr ◽  
Cecilia Amelia Fazzio Escanhoela
Keyword(s):  
Bile Duct ◽  
Morphological Study ◽  
Wistar Rats ◽  
Hepatic Duct ◽  
Control Group ◽  
Sham Operation ◽  
Biliary Cirrhosis ◽  
Technical Failure ◽  
Duct Obstruction ◽  
Duct Ligation

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

scholarly journals Effect of Carica Papaya L Leaf Infusion on Transporting Glucose across Small Intestine Cell Membrane of Wistar Rats Model

2019 ◽  
Vol 6 (1) ◽  
pp. 42-45
Author(s):  
Srisathis Renganathan ◽  
Vycke Yunivita ◽  
Anna Martiana S ◽  
Nida Suraya
Keyword(s):  
Small Intestine ◽  
Cell Membrane ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Intestine Cell

scholarly journals Histogenesis and Histomorphometric study of Human Fetal Small Intestine

1970 ◽  
Vol 29 (6) ◽  
Author(s):  
Salva MN ◽  
Chandni Gupta ◽  
Arvind Kumar Pandey ◽  
Nitesh Kumar ◽  
Sushma R Kotian ◽  
...  
Keyword(s):  
Small Intestine ◽  
Normal Growth ◽  
Later Life ◽  
First Trimester ◽  
Second Trimester ◽  
Third Trimester ◽  
Histomorphometric Study ◽  
Microscopic Features ◽  
Early Life Events ◽  
Muscularis Externa

Background: Intestine plays a major role for the normal growth of the fetus during the prenatal period. The process of the embryonic development is not quantified histologically. Therefore the main aim of the study was to measure the thickness of all part of the wall of the small intestine that are mucosa, submucosa and muscularis externa and to look for the appearance of the Brunner’s glands and Peyer’s patches in the submucosa of duodenum and ileum.Methods: The present study was carried out on 30 fetuses of gestational ages ranging from 11-36 weeks. Ten fetuses from each trimester were used in the study. Fetal small intestine were dissected carefully, and were separated as duodenum, jejunum & ileum and fixed in formalin solution. The tissue was processed for histology and then slides were stained with Haematoxylin and Eosin. The microscopic features were noted using light microscope.Results: The thickness of the mucosa, submucosa and the muscularis externa was observed to be increased in first trimester, decreased in the second trimester and again increased in the third trimester, which could be because of the increase cell turnover and the arrangement of the collagen fibers as to support the mucosa and the muscularis externa.Conclusion: Thus, the knowledge of the histogenesis and histomorphometry of the human fetal small intestine is crucial for the adult gastroenterologist to appreciate, because of the potential for these early life events to affect the responsiveness of the intestine to physiological or pathological challenges in later life. 

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