Detection of metalloβ-lactamase genes in heteroresistant Pseudomonas aeruginosa isolated from clinical isolates in Egypt

The aim of the study is to isolate and characterize Pseudomonas aeruginosa recovered from different clinical specimens and then study the susceptibility of the isolated strains to different antibiotics, screening for heteroresistant isolates and detecting the metalloβ-lactamase genes in these isolates. A total of two hundred and fifty clinical isolates were collected, from which one hundred forty five isolates revealed Pseudomonas aeruginosa. They were collected from different clinical specimens applied for bacteriological testing from hospitalized in-patients admitted to Kasr Al Aini Hospital and Al-Demerdash Hospital, Egypt, in the period from February 2016 to December 2017. Antibiotic susceptibility testing was done using ten antibiotics. The study covered the heteroresistance of P.aeruginosa towards several classes of antibiotics to make the statistical analysis convenient and to overview the significance of this resistance. The minimum inhibitory concentration was detected for the heteroresistant P.aeruginosa resistant isolates. The polymerase chain reaction of the heteroresistant strains was performed to detect the metallo-β-lactamase genes SIM, IMP and SPM and then sequencing was done consequently. SIM, IMP and SPM metalloβ-lactam e genes were detected in the heteroresistant isolates. The isolates showed a high resistance pattern to ampicillin (98.62%) and a high sensitivity rate to imipenem (96.55%) and the IMP gene was the highly significant gene.

DETEKSICEMARAN BAKTERIOLOGIS DAN LOGAM BERAT DAGING LANGKITANG (Faunus ater)

This study aims to determine the level of bacteriological contamination and heavy metal lead (Pb) in langkitang (Faunus ater) meat. The sample were taken randomly as 15 samples from traders. Examination of microbial contamination in meat used a serial dilution method Most Probable Number (MPN) consisting of the presumptive test, confirmed test, and completed test. Measurement of Lead Heavy Metal (Pb) content used Atomic Absorption Spectrophotometry (AAS). The result of bacteriological testing of the MPN/ 100 ml sample showed that the tested Langkitang meat contained contaminating bacteria for each sample tested, and Lead metal was also detected, some of which had values that exceeded the threshold. Based on the results of the analysis, the langkitang meat has a microbial and lead metal contamination content that exceeds the health threshold so that it takes proper processing before the meat is consumed

Examining the factors related to bacteriological testing of private wells in Southern Ontario

The incidence of infectious waterborne disease in Canada continues to be a public health issue and can be associated with the source of drinking water. Millions of Canadians relying on unregulated private well water are at increased risk of disease. This study examined relationships between well and owner characteristics and the frequency of microbial testing of private wells in two southern-Ontario counties. Using multi-level logistic regression models, testing frequency (i.e., at least once per year vs. less) was modeled, as both self-reported and laboratory-validated, for associations with owner and well characteristics. For the self-reported outcome, a previous adverse test result significantly increased the odds of being classified as a frequent tester, and owners with a well-head more than 16 inches (40.6 cm) above the ground were at significantly higher odds of being classified as frequent testers compared to those with well-heads less than 16 inches above the ground and those below ground level. For the model based on the laboratory-validated outcome, the odds of an owner being a frequent tester significantly varied with the length of occupancy and the occurrence of a previous adverse result. The absence of associations between other well characteristics and testing frequency suggests that well safety education could benefit these communities.

Splenic abscess as a rare symptom of the extrapulmonary tuberculosis – case report

Introduction: Extrapulmonary tuberculosis can involve any organ or tissue. It is a rare disease in the Czech Republic with an incidence rate of 0.62 cases per 100.000 persons. It affects mostly immunocompromised patients. The most common sites include lymph nodes, the urogenital system, skin, joints, bones and serous epithelium – the peritoneum, pleura, and pericardium. Splenic involvement is rare. Mycobacterium is a slow growing intracellular parasite. The diagnostic process is very difficult; microbiological diagnosis is critical. Case report: An 84 years old female patient with subcapsular splenic rupture with no trauma history as a cause of anemia. Splenic abscess was diagnosed during surgical revision and splenectomy. Tuberculosis was suspected based on subsequent histological analysis, which was confirmed after nine weeks of peritoneal fluid culture. The surgical procedure and postoperative hospitalization were not associated with any complications. The patient was referred to the respiratory clinic for further treatment. Conclusion: The diagnosis of extrapulmonary tuberculosis including splenic localization should always be considered. A sample from the affected tissue or effusion must be collected in the case of unclear perioperative findings and sent for complete bacteriological testing, including mycobacterial culture. If a tuberculous splenic abscess is found, the therapeutic process should involve its complete drainage in combination with long-term anti-TB medication.