Finger Printing of Rahmani, Chios Sheep Breeds and Their Crosses Using Random Amplified Polymorphic Dna (Rapd)

The Australian-bred lucerne cultivars, Trifecta and Sequel, were found to possess useful levels of resistance to both Colletotrichum trifolii races 1 and 2. Race 2 has only been previously observed in the United States and surveys did not reveal its presence in Australia. Multilocus finger printing using random amplified polymorphic DNA (RAPDs) analysis revealed low diversity (<10% dissimilarity) within Australian C. trifolii collections, and between the Australian race 1 isolates and a US race 2 isolate. Studies on the inheritance of resistance to C. trifolii race 1 in individual clones from Trifecta and Sequel revealed the presence of 2 different genetic mechanisms. One inheritance was for resistance as a recessive trait, and the other indicated that resistance was dominant. The recessive system has never been previously reported, whereas in the US, 2 completely dominant and independent tetrasomic genes An1 and An2 have been reported to condition C. trifolii resistance. It was not possible to fit the observed segregations from our studies to a single-gene model. In contrast to US studies, clones of cv. Sequel exhibiting the recessive resistance reacted differently to spray and stem injection with C. trifolii inoculum, being resistant to the former and susceptible to the latter, providing additional evidence for the presence of a different genetic mechanism conditioning resistance to those previously reported in the US. As C. trifolii is one of the most serious diseases of lucerne worldwide, the future development of molecular markers closely linked to the dominant and recessive resistances identified in these studies, and the relationships between these resistances and An1 and An2 as determined by genetic mapping, appear to be useful areas of future study.

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GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT

Indonesian Aquaculture Journal ◽

10.15578/iaj.7.1.2012.1-10 ◽

2016 ◽

Vol 7 (1) ◽

pp. 1

Author(s):

Andi Parenrengi ◽

Andi Tenriulo ◽

Syamsul Alam Ali

Keyword(s):

Genetic Variability ◽

Random Amplified Polymorphic Dna ◽

Similarity Index ◽

Marine Species ◽

Published Data ◽

Arbitrary Primers ◽

Flying Fish ◽

Upgma Cluster Analysis ◽

Finger Printing ◽

Species Specific

Flying fish, Hirundichthy oxycephalus is one of economically important marine species to Indonesia, particularly in Makassar Strait and Flores Sea. However, there is a limited published data on genetic variation in molecular marker level of this species. Random Amplified Polymorphic DNA (RAPD) was employed in this study to determine the genetic variability of three populations of flying fish collected from Takalar, Pare-Pare, and Majene in Makassar Strait. Genomic DNA was isolated from preserved muscle tissue using phenol-chloroform technique. Two selected arbitrary primers (CA-01 and P-40) were performed to generate RAPD finger printing of flying fish populations. The two primers generated a total of 81 fragments (loci) and 50 polymorphic fragments with size ranging from 125 to 1,250 bp. There were no significant differences in number of fragment and number of polymorphic fragment among populations. The high polymorphism (63.5±7.4%) was obtained from Takalar population followed by Pare-Pare (58.3±19.6%) and Majene population (57.7±0.8%). Similarity index of individuals was 0.60±0.17 for Takalar, 0.63±0.17 for Majene and 0.75±0.21 for Pare-Pare population. Seven fragments were identified as species-specific markers of H. oxycephalus. The UPGMA cluster analysis showed that the Takalar population was genetically closer to Pare-Pare population (D= 0.0812) than to Majene population (D= 0.1873).

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Seasonal variation of wool fibre length in Karagouniko and Chios sheep in relation to meteorological factors

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2019.v31.i10.2020 ◽

2019 ◽

pp. 788

Author(s):

Aristidis Matsoukis

Keyword(s):

Meteorological Factors ◽

Fibre Length ◽

Meteorological Variables ◽

Wool Fibre ◽

New Horizons ◽

Sheep Breeds ◽

Length Growth ◽

Chios Sheep ◽

The Impact ◽

Opening Up

Karagouniko and Chios sheep breeds present a lot of advantages on the implementation of sheep farming in Greece, a country with diverse relief resulting in a microclimatic variety, but nothing is known about the impact of season, as the outcome of important meteorological variables, on the fibre length growth (FLG) of the aforementioned breeds. Thus, the effect of season (Winter, Spring, Summer, Autumn) on the FLG of these breeds (by using analysis of variance) was studied in the Artificial Insemination Center of Karditsa (39021΄18΄΄N, 21054΄19΄΄E), Periphery of Thessaly, Greece, combined with a correlation analysis between FLG of each examined sheep breed and each of the studied meteorological variables, air temperature (AIRT), relative humidity (RH), sunshine (SUNS) and rainfall (R) for a two-year period. It was found that the FLG of Karagouniko sheep was significantly higher than the respective growth of Chios sheep, for each examined season, while the descending order of seasonal FLG for both breeds was Winter>Spring>Autumn>Summer. Fibre length growth of Karagouniko and Chios breeds correlated negatively with AIRT and SUNS and positively with RH, implying a better FLG in cooler, more overcast and wetter time periods. Our study adds new knowledge concerning the effect of season, and particularly, the effect of the aforementioned meteorological variables on the wool growth of two considerable sheep breeds in Greece, Karagouniko and Chios, opening up new horizons for their exploitation.

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Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

Progressive Agriculture ◽

10.3329/pa.v20i1-2.16839 ◽

2013 ◽

Vol 20 (1-2) ◽

pp. 1-8

Author(s):

MM Rahman ◽

L Rahman ◽

SN Begum ◽

F Nur

Keyword(s):

Genetic Distance ◽

Gene Diversity ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Molecular Genetic Analysis ◽

Polymorphic Loci ◽

Rice Genotypes ◽

High Level ◽

Genetic Diversity Study ◽

Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Neis genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 8, 2009

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PERFORMANCE OF TORIA (BRASSICA COMPESTRIS SYN. RAPA L.) VARIETY: RAJ VIJAY TORIA-2 IN CENTRAL, EASTERN AND NORTH-EASTERN STATES OF INDIA UNDER RAINFED CONDITION

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp263-269 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

VIMLESH KUMAR TIWARI

Keyword(s):

Seed Yield ◽

Rainfed Condition ◽

Significant Difference ◽

North Eastern ◽

Finger Printing ◽

Quantitative And Qualitative Traits ◽

Central Eastern ◽

Dna Finger Printing ◽

Mature Seeds ◽

Weight Trait

Performance of developed genotype RMT 08-2 was evaluated in central, eastern and north-eastern states of India under rain-fed condition for quantitative and qualitative traits. It gave highest seed yield over checks in zone III and V. Morphologically plants were erect, medium spreading in nature and primary branches with dichotomous habit. Plants height ranged from 107124 (cm) which matured in 82-112 days. Mature seeds were round in shape and blackish brown in colour. No significant difference between RVT-2 and checks were observed for test weight trait. An average oil yield 485 (kg/h) was recorded over 7 places which was 10% higher than both checks i.e. 14.12% and 11.24% under AICRP trials. Maximum seed yield was obtained on farmers field during 2013-14 and 2014-15 which was 1500 (kg/h) and 1215 (kg/h) that is 33.42% and 26.30% respectively over farmers own seeds. At Morena center, highest seed yield (1753 kg/h) over Bhawani (1512 kg/h) was 15.94% higher than check whereas RVT-2 gave 2245 (kg/h) against Bhawani (1975 kg/h) which was 13.67% higher. DNA finger printing indicated that primers PUT-19, PUT-96, PUT-149, PUT-169, PUT-181 and PUT-271 are useful in generating unique profile of RVT-2 containing 27 bands for its discrimination from other varieties.

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EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

FLORA AND FAUNA ◽

10.33451/florafauna.v23i2pp461-467 ◽

2017 ◽

Vol 23 (2) ◽

Cited By ~ 1

Author(s):

SUNITA BORDE ◽

ASAWARI FARTADE ◽

AMOL THOSAR ◽

RAHUL KHAWAL

Keyword(s):

Fresh Water ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Band Pattern ◽

Genomic Variation ◽

Polymorphic Band ◽

Rapd Profile ◽

Band Size ◽

Pcr Product ◽

The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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