PENGARUH EKSTRAK KASAR BUAH PALA MYRISTICA ARGENTEA TERHADAP PERTUMBUHAN IKAN KOI CYPRINUS CAPRIO

Ikan koi di Indonesia merupakan ikan hias favorit dan banyak digemari masyarakat luas karena tubuhnya yang mempesona dan harganya relatif tidak terlalu mahal. Ikan koi sekarang ini masih menjadi salah satu komoditas perdagangan yang cukup baik dalam bidang perikanan. Tujuan dari penelitian ini adalah untuk mengetahui Pengaruh Ekstrak Kasar Buah Pala Myristica Argentea Terhadap Pertumbuhan Ikan Koi Cyprinus Caprio. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) ,dengan empat perlakuan dan tiga ulangan yaitu perlakuan A (Dosis 5% ekstrak kasar buah pala), perlakuan B (Dosis 10% ekstrak kasar buah pala), perlakuan C (Dosis 15% ekstrak kasar buah pala) dan perlakuan D ( Dosis 0 % ekstrak kasar buah pala). Parameter uji berupa lajui pertumhuhan bobot dan pertumbuhan panjang. Hasil penelitian menunjukkan bahwa ekstrak Kasar Daging Buah Pala myristica argentea memberikan pengaruh yang baik terhadap pertumbuhan ikan ko setelah diberikan perlakuan.. Dosis yang baik untuk pertumbuhan berat dan panjang ikan koi ialah dosis 10% - 5%. Koi fish in Indonesia is a favorite ornamental fish and much-loved by the wider community because of its charming body and relatively inexpensive price. Koi fish is still one of the trade commodities that is quite good in the field of fisheries. The purpose of this study was to determine the Effect of Crude Extract of Nutmeg Myristica Argentea on the Growth of Cyprinus Caprio Koi Fish. The research method used was a completely randomized design (CRD), with four treatments and three replications, namely treatment A (5% dose of nutmeg crude extract), treatment B (10% dose of nutmeg crude extract), treatment C (15% dose of nutmeg extract). crude nutmeg) and treatment D (Dose of 0% crude extract of nutmeg). Test parameters in the form of weight growth rate and length growth. The results showed that the crude extract of the Nutmeg myristica argentea had a good effect on the growth of koi fish after being given treatment. A good dose for the growth of weight and length of koi fish is a dose of 10% - 5%

The application of integrated multi-trophic aquaculture (IMTA) using floating net cages on Tilapia fish with native fish (Peres, Lemeduk, and Depik)

Environmental problems due to aquaculture occur along with the increase in aquaculture production. IMTA is one system used as a solution in dealing with environmental problems. The objective of this study was to analyze the growth performance, survival rate, and production of the main commodity (tilapia) and the supporting commodities (Peres, Lemeduk, and Depik) which are applied through the application of integrated multi-trophic aquaculture (IMTA) using floating net cages. This research was conducted at the Lukup Badak Fish Seed Center, Central Aceh from July to August 2020. The experimental design used was a completely randomized design (CRD) with 4 treatments and 3 replications, namely: A (tilapia), B (tilapia and peres), C (tilapia and lemeduk), and D (tilapia and depik). ANOVA test results showed that the IMTA system had a significant effect on absolute length growth, absolute weight growth, specific growth rate (SGR), survival rate, and biomass production of tilapia (P0.05). The results showed that maintenance for 42 days produced the highest value in treatment B with the increase in absolute length growth (4.26cm±0.24); absolute weight growth (5.47g±0.45), specific daily growth rate (2.28%/day±0.13) and the highest tilapia biomass production (480g/0.5m2±19.25). The highest survival rate was found in treatment C (82.42%±2.28). It was concluded that the treatment using the IMTA system was better than without the IMTA system.Keywords:IMTAWater qualityGrowth performanceTrophic interactionBiomass production

INNOVATION OF SINBIOTIC FORMULA FOR THE GROWTH OF WHITE SHRIMP LARVAE (Litopenaeus vannamei)

In the hatchery of white shrimp larvae, there are some problems, such as a decreased water quality and diseases caused by bacteria which cause a decrease in shrimp growth. The solution to reduce these problems is by applying synbiotics. This research aimed to make an innovative synbiotic formula for the absolute length growth and survival rate of white shrimp larvae (Litopenaeus vannamei) as well as the total number of bacteria and the number of Vibrio sp. In this study, there were four treatments, as follows: C-: negative control (without synbiotic application) C+: positive control (commercial synbiotic) P: probiotic 2.5 x 105 cfu/ml and prebiotic 1,5 ppm Q: probiotic 5 x 105 cfu/ml and prebiotic 1 ppm R: probiotic 7,5 x 105 cfu/ml and prebiotic 0,5 ppm S: probiotic 106 cfu/ml and prebiotic 2 ppm, each treatment with four repetitions. This research was set up in a completely randomized design experiment using twenty-four plastic tanks with 50 L total volume filled with 20 L sterile sea water and stocked with 4,000 nauplii in the PT. Citra Larva Cemerlang hatchery, Kalianda, Lampung. Variables observed in this research were survival rate, absolute length growth, total bacterial and Vibrio sp. counts, and water quality in the white shrimp larvae (Litopenaeus vannamei). The results showed that the best survival rate is in treatment S (probiotic 106 cfu/ml and prebiotic 2 ppm) (87.7%), the highest absolute length growth is in treatment P (3.8 mm), the highest total bacteria was in treatment S (11.1 log cfu/ml), and the best total Vibrio sp. is in treatment S (3.5 log cfu/ml). Water quality of the six treatments shows results that are still in normal conditions following Indonesian National Standard SNI 7311: 2009.

Time Course of Perceived Visual Distortion and Axial Length Growth in Myopic Children Undergoing Orthokeratology

Purpose: To establish the time course of the subjective visual function changes during the first month of orthokeratology treatment in myopic children, and to investigate how the time course variations are associated with the objective optical quality changes and the axial length growth (ALG) after 1 year of treatment.Methods: A total of 58 myopic children aged from 8 to 16 years participated in this self-controlled prospective study. All subjects were fitted with designed spherical four-zone orthokeratology lenses. Subjective visual function was evaluated with orientation discrimination threshold (ODT), and objective optical quality was quantified with the high-order aberration root-mean-square (HOA-RMS) and the changing speed of HOA. The measurements were done before the lens fitting and 1 day, 1-, 2-, and 4-weeks after lens wear. Axial length was obtained at baseline and 1-year follow-up, and ALG was defined as the difference. One-way ANOVA was conducted to compare the difference for statistical analysis.Results: After lens fitting, the ODT time courses peaked on day 1 in 28 children, 1 week in 15 children, 2 weeks in 11 children, and 4 weeks in 4 children. In contrast, the HOA-RMS steadily rose during the first month, and the changing speed of HOA was only transiently elevated on day 1 after the initial lens wear. The ALG was 0.12 ± 0.20 mm in subjects whose ODT peaked at day 1, 0.08 ± 0.09 mm in subjects whose ODT peaked on 1-week, and 0.12 ± 0.15 mm in subjects whose ODT peaked on 2-week or later. There was no difference in axial growth among the subjects whose ODT peaked at different days (P = 0.734).Conclusion: While half ODT time course resembled the changing speed of HOA with a transient elevation on day 1, about a quarter of the ODT time course resemble the steadily rising of HOA-RMS, and the rest was located in the middle. The ALGs in children with different types of ODT time courses were similar.

Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?

Fission yeast is commonly used as a model organism in eukaryotic cell growth studies. To describe the cells’ length growth patterns during the mitotic cycle, different models have been proposed previously as linear, exponential, bilinear and biexponential ones. The task of discriminating among these patterns is still challenging. Here, we have analyzed 298 individual cells altogether, namely from three different steady-state cultures (wild-type, wee1-50 mutant and pom1Δ mutant). We have concluded that in 190 cases (63.8%) the bilinear model was more adequate than either the linear or the exponential ones. These 190 cells were further examined by separately analyzing the linear segments of the best fitted bilinear models. Linear and exponential functions have been fitted to these growth segments to determine whether the previously fitted bilinear functions were really correct. The majority of these growth segments were found to be linear; nonetheless, a significant number of exponential ones were also detected. However, exponential ones occurred mainly in cases of rather short segments (<40 min), where there were not enough data for an accurate model fitting. By contrast, in long enough growth segments (≥40 min), linear patterns highly dominated over exponential ones, verifying that overall growth is probably bilinear.

The Influence of Feeding Combination Silkworm (Tubifex sp.) with Commercial Feed on Growth Performance of Catfish (Clarias sp.)

This study aims to determine the growth of catfish (Clarias sp.) Seeds by giving a combination of silkworms (Tubifex sp.) and commercial feed. This research was conducted in August - October 2020 in the fish farming group of PT ASABRI, West Ilir II Palembang, South Sumatera. The treatments used in this study were 100% silkworm (control) feeding and a combination of feed treatment consisting of 75% commercial feed and 25% silkworms. Parameters include absolute weight growth, absolute length growth, feed efficiency, survival, and water quality. The results obtained from this study indicate that combination feeding (P1) obtained better results than the control (P0) with absolute weight growth data of 8.38 g, absolute length 7.39 cm, feed efficiency 56.38% and survival 87.50%.Keywords: catfish, commercial feed, combination, growth silkworm

Use baseline axial length measurements in myopic patients to predict the control of myopia with and without atropine 0.01%

Purpose Identifying axial length growth rate as an indicator of fast progression before initiating atropine 0.01% for myopia progression in children. Method From baseline, axial length growth over six months was measured prospectively. Subjects were then initiated on atropine 0.01% if axial length growth was greater than 0.1mm per 6 months (fast progressors), axial length and spherical equivalent change measurements recorded every six months. The rate of change was compared to the baseline pre-treatment rate. If axial length change was below the threshold, subjects received monitoring only. Results 73 subjects were identified as fast progressors and commenced atropine 0.01%, (mean baseline refraction of OD -2.9±1.6, OS -2.9±1.8 and a mean baseline axial length OD 24.62 ± 1.00 mm, OS 24.53 ± 0.99 mm). At six months, the mean paired difference of axial length growth rate was significantly reduced by 50% of baseline (all 73 subjects, p<0.05). 53 subjects followed to 12 months, and 12 to 24 months maintained a reduced growth rate. Change in mean spherical equivalent was significantly reduced compared to pre-treatment refractive error (mean paired difference p<0.05) and at each subsequent visit. 91 children were slow progressors and remained untreated. Their axial length growth rate did not change significantly out to 24 months. Spherical equivalent changed less than -0.5D annually in this group. Conclusion Identifying fast progressors before treatment initiation demonstrated a strong treatment effect with atropine 0.01% reducing their individual rate of myopia progression by 50%. Another large group of myopic children, slow progressors, continued without medical intervention. A baseline axial length growth rate is proposed as a guideline to identify fast progressors who are more likely to benefit from atropine 0.01%.

The Effect of Different Size Recirculation Systems on the Quality of Water in Tilapia (Oreochromis niloticus) Culture

This study aims to analyze the effect of different cross-sectional areas of recirculation systems on water quality in tilapia (Oreochromis niloticus) rearing. The method used is an experimental method with a completely randomized design (CRD), 4 treatments and 3 replications each. The treatments are P0 (conventional), P1 (2 cross-sectional units with an area of 1,087 cm2), P2 (4 section units with an area of 2,174 cm2), P3 (6 section units with an area of 3,261 cm2). Each treatment used the same filter, namely cotton, zeolite, pumice stone, and bio balls. The results of the research the highest absolute weight growth was obtained in the P3 treatment with an average value of 4.56 g and followed respectively P2 4.38 g, P2 3.03, P0 2.59 g. The highest absolute length growth rate was obtained in treatment P3 2.71 cm, P2 of 2.54 cm, P1 of 1.87 cm, and the lowest absolute length was found in treatment P0 of 1.62 cm. The highest feed conversion value was at P0 at 3.91, followed by P1 at 2.84, P2 at 2.06, and the lowest at P3 at 1.94. The highest survival value was P3 of 85.00, P2 r 83.33, P1 73.33 and the lowest was P0 of 55.00. The highest diversity coefficient value was obtained in treatment P0 of 17.58, followed respectively by treatment P1 of 16.05, P2 of 12.91 and the lowest coefficient of diversity found in treatment P3 of 12.69. The conclusion of this study is the use of different recirculation cross-sectional areas has a significant effect on absolute weight growth, absolute length growth, feed conversion ratio, survival, and water quality. The recirculation cross-sectional area in P3 treatment with a cross-sectional area of 3.261 cm2 gave good results for the survival of tilapia (O. niloticus).

A retrospective analysis of the therapeutic effects of 0.01% atropine on axial length growth in children in a real-life clinical setting

Background Several randomized controlled studies have demonstrated the beneficial effects of 0.01% atropine eye drops on myopia progression in children. However, treatment effects may be different in a routine clinical setting. We performed a retrospective analysis of our clinical data from children to investigate the effect of 0.01% atropine eye drops on myopia progression in a routine clinical setting. Methods Atropine-treated children were asked to instill one drop of 0.01% atropine in each eye every evening at 5 days a week. Myopic children who did not undergo atropine treatment served as controls. Objective refraction and ocular biometry of 80 atropine-treated and 103 untreated children at initial visit and 1 year later were retrospectively analyzed. Results Myopic refractions in the treated and untreated children at initial visit ranged from −0.625 to −15.25 D (−4.21 ± 2.90 D) and from −0.125 to −9.375 D (−2.92 ± 1.77 D), respectively. Ages at initial visit ranged from 3.2 to 15.5 years (10.1 ± 2.7 years) in the treated and from 3.4 to 15.5 years (11.2 ± 3.0 years) in untreated children. Two-factor ANOVA for age and atropine effects on axial length growth confirmed that axial length growth rates declined with age (p<0.0001) and revealed a significant inhibitory effect of atropine on axial length growth (p<0.0015). The atropine effect on axial length growth averaged to 0.08 mm (28%) inhibition per year. Effects on refraction were not statistically significant. Conclusion The observed atropine effects were not very distinctive: Statistical analysis confirmed that atropine reduced axial length growth, but to an extent of minor clinical relevance. It was also shown that beneficial effects of 0.01% atropine may not be obvious in each single case, which should be communicated with parents and resident ophthalmologists.