scholarly journals Evaluation of anti-Schistosoma mansoni igG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment

2001 ◽  
Vol 43 (3) ◽  
pp. 153-159 ◽  
Author(s):  
Célia Maria V. VENDRAME ◽  
Márcia Dias T. CARVALHO ◽  
Célia Regina F. YAMAMOTO ◽  
Maria Cristina NAKHLE ◽  
Silvino Alves CARVALHO ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Specific Treatment ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Igg Antibodies ◽  
Schistosomiasis Mansoni ◽  
Before And After ◽  
Precipitin Test ◽  
Egg Antigen ◽  
Adult Worm Antigen

The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtained. Significant decreases in IgG1, IgG3 and, mainly, IgG4, but not anti-SEA IgG2 levels were observed six months after treatment, again without negativity. Analysis of anti-AWA IgG antibodies by immunoblotting before treatment showed a 31 kDa strand in 14 patients (82%) which disappeared in three cases up to six months after treatment; furthermore, anti-SEA IgG antibodies showed the same band in nine patients (53%) before treatment, which disappeared in only four cases up to six months after treatment.

scholarly journals Schistosoma mansoni circulating polysaccharide and protein antigens recognized by sheep antisera in patients with different clinical forms of schistosomiasis before and after treatment

1994 ◽  
Vol 36 (4) ◽  
pp. 321-325
Author(s):  
Julia Maria Costa-Cruz ◽  
Sumie Hoshino-Shimizu ◽  
Antonio Walter Ferreira ◽  
Mario E. Camargo ◽  
Thales de Brito ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Igg Antibodies ◽  
Protein Antigens ◽  
Clinical Forms ◽  
Schistosomiasis Mansoni ◽  
Before And After ◽  
Circulating Antigen ◽  
Circulating Antigens ◽  
Specific Igg ◽  
Specific Igg Antibodies

Two sheep antisera, one of which raised against polysaccharide (Po) and other against protein (Pt) components of Schistosoma mansoni adult worms, were assessed by ELISA for their ability to detect circulating parasite antigens in patients with different clinical forms of chronic schistosomiasis mansoni. The former antiserum detected parasite antigens in liver granulomata and the latter in renal glomeruli from schistosomiasis patients and mice experimentally infected with S. mansoni. In general, the levels and/or positivity rate of circulating antigens and specific IgG antibodies were significantly higher in patients with hepatointestinal (HI) and hepatosplenic (HS) forms than in mild intestinal (I) forms. An association between Po antigens and clinical features of the disease was observed, as the level of these antigens was low (137 ng/ml) as well as the positivity rate (7.9%) in patients with I forms; values that were intermediate (593 ng/ml and 33.3%) in those with HI forms, and high (1.563 ng/ml and 50.0%) in more severe HS forms. The Pt antigens were detected in the studied clinical forms not differing statistically but, the positivity rate was significantly higher in HS forms comparatively to I forms. The antisera studied revealed distinct circulating antigen profiles, and the prognostic value of Po and Pt antigens was suggested.

Oxydative stress markers and cytokine levels in rosuvastatin-medicated hypercholesterolemia patients

2018 ◽  
Vol 44 (4) ◽  
pp. 530-538
Author(s):  
Aysun Çetin ◽  
İhsan Çetin ◽  
Semih Yılmaz ◽  
Ahmet Şen ◽  
Göktuğ Savaş ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Interleukin 1 ◽  
Paraoxonase 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Phenotypic Effect ◽  
Necrosis Factor Alpha ◽  
Stress Markers ◽  
Tnf Α ◽  
Before And After

Abstract Background Limited research is available concerning the relationship between oxidative stress and inflammation parameters, and simultaneously the effects of rosuvastatin on these markers in patients with hypercholesterolemia. We aimed to investigate the connection between cytokines and oxidative stress markers in patients with hypercholesterolemia before and after rosuvastatin treatment. Methods The study consisted of 30 hypercholesterolemic patients diagnosed with routine laboratory tests and 30 healthy participants. The lipid parameters, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), paraoxonase-1 (PON1) and malondialdehyde (MDA) levels in controls and patients with hypercholesterolemia before and after 12-week treatment with rosuvastatin (10 mg/kg/day), were analyzed by means of enzyme-linked immunosorbent assay. Results It was found that a 12-week cure with rosuvastatin resulted in substantial reductions in IL-1β, IL-6 and TNF-α and MDA levels as in rising activities of PON1 in patients with hypercholesterolemia. Before treatment, the PON1 levels were significantly negatively correlated with TNF-α and IL-6 in control group, while it was positively correlated with TNF-α in patients. Conclusion Our outcomes provide evidence of protected effect of rosuvastatin for inflammation and oxidative damage. It will be of great interest to determine whether the correlation between PON1 and cytokines has any phenotypic effect on PON1.

scholarly journals PREPARATION AND EVALUATION OF CHEMICALLY INACTIVATED SALMONELLA ENTERITIDIS VACCINE IN CHICKENS

2017 ◽  
Vol 10 (11) ◽  
pp. 341
Author(s):  
Mounir M El-safty ◽  
Hala Mahmoud ◽  
Eman Sa Zaki ◽  
Howaida I Abd-alla
Keyword(s):  
Immune Responses ◽  
Salmonella Enteritidis ◽  
Serum Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Chemically Induced ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Cell Envelopes

  Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine).Results: Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH.Conclusion: It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

scholarly journals Vasculotoxicity of Chemotherapy: Assessment оf Endothelial Dysfunction Biomarkers’ Levels in Gastric Cancer Patients

Kardiologiia ◽  
2020 ◽  
Vol 60 (5) ◽  
pp. 35-40
Author(s):  
Yu. Yu. Kirichenko ◽  
Yu. N. Belenkov ◽  
E. V. Privalova ◽  
Yu. I. Naymann ◽  
E. P. Gitel ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Healthy Volunteers ◽  
Stomach Cancer ◽  
Specific Treatment ◽  
Study Groups ◽  
Main Group ◽  
Control Group ◽  
Instrumental Evaluation ◽  
Oncological Patients ◽  
Before And After

Aim To evaluate dynamics of biomarkers for endothelial dysfunction (ED), including endothelin-1 (ET-1) and von Willebrand factor (VWF) in patients with stomach cancer (adenocarcinoma) before and after polychemotherapy (PCT); to compare these results with respective values in healthy volunteers and patients with cardiovascular diseases (CVD); to study correlations of the ED biomarkers with indexes of instrumental evaluation of endothelial dysfunction.Material and methods The study included 75 participants, including 25 healthy volunteers (control group), 25 patients with documented CVDs (arterial hypertension + ischemic heart disease), and 25 patients of the main group with histologically documented stage II-IV stomach cancer (adenocarcinoma) who received different courses of PCT with platinum-based agents (oxaliplatin, cisplatin) and fluoropyrimidines (5 fluorouracil, capecitabin). Laboratory measurement of ED biomarkers, computerized nailfold video capillaroscopy (CNVC), and finger laser photoplethysmography (PPG) (methods for noninvasive evaluation of vascular wall and ED), electrocardiography, 24-h ECG Holter monitoring, and echocardiography (EchoCG) were performed for all patients of the main group prior to PCT and within one months after the last course completion. This evaluation was performed once for healthy volunteers and patients of the CVD group upon inclusion into the study.Results In the main group, ET-1 levels were non-significantly lower than normal and did not change during the courses of antitumor treatment (0.95 [0.6; 1.4] and 0.94 [0.7; 1.4] pg /ml (р<0.9) before and after PCT, respectively). Statistically significant differences were found between the control group and oncological patients after the treatment (р<0.04). Levels of VWF remained within the normal range in all examined participants and did not significantly differ between study groups, including oncological patients before and after the specific treatment (р>0.05 for all comparisons). The correlation analysis detected significant correlations of ET-1 levels with functional disorders of microcirculation, ET-1 with the occlusion index (rs=0.56; p=0.005), ЕТ-1 with percentage of capillary restoration (PCR, rs= –0.72; p=0.018) and with the incidence rate of supraventricular extrasystole (rs=0.48; p=0.032).Conclusion The dynamics of ED biomarkers was studied for the first time in patients with stomach cancer receiving a specific antitumor therapy. Although no significant changes in ЕТ-1 and VWF were observed during the PCT (probably due to exhaustion of the endothelial system and a small patient sample), these indexes can be considered as early vasculotoxicity markers due to the presence of significant correlations with indexes of impaired endothelial function according to the results of instrumental evaluation.

Comparison of salivary IgA and systemic IgA and IgG antibodies to Saccharomyces cerevisiae in HIV-infected subjects

2003 ◽  
Vol 14 (7) ◽  
pp. 458-462 ◽  
Author(s):  
Maria Belazi ◽  
Alexandra Fleva ◽  
Drakoulis Drakoulakos ◽  
Despina Panayiotidou
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid Phase ◽  
Systemic Disease ◽  
Test Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Antibody Activity ◽  
Igg Antibodies ◽  
Whole Saliva ◽  
Significant Difference

Our objective was to investigate the concentrations of IgA and IgG antibodies to Saccharomyces cerevisiae in whole saliva and serum samples from HIV-infected patients and to compare them with the corresponding antibody values of healthy controls. A cross-sectional design was used. The test group consisted of 23 HIV-infected male individuals, aged 20-41 years old, free of any other systemic disease. Twenty healthy subjects aged 27-43 years old served as controls. Whole unstimulated saliva and blood were collected from all subjects. IgA concentrations in saliva and IgA and IgG concentrations in serum were measured by solid-phase enzyme-linked immunosorbent assay. Salivary antibody concentrations were calculated by reference to a pooled standard saliva obtained from 10 healthy males with high levels of anti- S. cerevisiae antibody activity. Total IgA and IgG concentrations were measured by nephelometry/tholocymetry assay. No significant difference was observed in salivary specific IgA and serum specific IgG levels to S. cerevisiae, while serum specific IgA were significantly lower in HIV infected patients compared to control group. Opportunistic infections due to S. cerevisiae, although rare, cannot be dismissed. This yeast can show a potential virulence in debilitated patients, therefore, further extensive investigation should be considered.

scholarly journals IL-1b, IL-6 and IL-8 Levels in Gyneco-Obstetric Infections

2005 ◽  
Vol 13 (4) ◽  
pp. 207-211 ◽  
Author(s):  
Beatriz Basso ◽  
Francisco Giménez ◽  
Carlos López
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Urinary Infection ◽  
Specific Treatment ◽  
Elisa Test ◽  
Post Treatment ◽  
Control Group ◽  
Third Trimester ◽  
Therapeutic Success ◽  
Before And After

Objective.During pregnancy cytokines and inflammatory mediators stimulate the expression of prostaglandin, the levels of which determine the onset of labor. The aim of this work was to study interleukin IL-1β, IL-6 and IL-8 levels in the vaginal discharge, serum and urine of pregnant women with genitourinary infection before and after specific treatment. One hundred and fifty-one patients were studied during the second or third trimester of their pregnancy.Methods.The selected patients were: healthy or control group (n= 52), those with bacterial vaginosis (n= 47), those with vaginitis (n= 37), those with asymptomatic urinary infection (n= 15) and post-treatment. The level of cytokines was assayed by ELISA test. The Mann–Whitney U-test was used for statistical analysis.Results.The IL-1βlevels in vaginal discharge were: control 103.5 ± 24.2 pg/ml, bacterial vaginosis 1030 ± 59.5, vaginitis 749.14 ± 66.7l (p< 0.0001), post-treatment 101.4 ± 28.7. IL-6 values were similar in both control and infected groups, and there were no patients with chorioamnionitis. In vaginal discharge IL-6: control 14.2 ± 3.9 pg/ml, bacterial vaginosis 13.2 ±3.8, vaginitis 13 ±4.2. IL-8 levels were: control 1643 ± 130.3 pg/ml, bacterial vaginosis 2612.7 ±257.7, vaginitis 3437 ± 460 (p< 0.0001), post-treatment 1693 ±126.6. In urine the results were: control 40.2 ±17 pg/ml, asymptomatic urinary infection 1200.7 ±375 (p< 0.0001). In patients with therapeutic success both IL-1βand IL-8 returned to normal levels.Conclusions.Genitourinary infections induce a significant increase in IL-1βand IL-8 levels in vaginal secretions, and IL-8 in urine as well. Both cytokines could be useful as evolutive markers of infection.

scholarly journals Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients

1995 ◽  
Vol 37 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Pedro Luiz Silva Pinto ◽  
Herminia Y. Kanamura ◽  
Rita Maria Silva ◽  
Cristina Renata Nardoto Rossi ◽  
Heitor Franco de Andrade Jr. ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Parasite Burden ◽  
Epidemiological Studies ◽  
Immunofluorescence Assay ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Previous Contact ◽  
Dot Elisa ◽  
Stool Analysis ◽  
Egg Antigen

Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies.

Expression of Interleukins in Patients with Nasal Polyposis

2005 ◽  
Vol 132 (4) ◽  
pp. 613-619 ◽  
Author(s):  
Richard Louis Voegels ◽  
Francini Grecco Melo De Pádua
Keyword(s):  
Surgical Treatment ◽  
Nasal Obstruction ◽  
Study Design ◽  
Immunosorbent Assay ◽  
Nasal Polyposis ◽  
Clinical Symptoms ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Fourth Decade ◽  
Before And After

OBJECTIVE: To correlate the levels of interleukins 1 β, 3, 4, and 5 before and after surgery and compare the levels between patients with and without recurrence of nasal polyposis. STUDY DESIGN AND SETTING: Thirty-nine patients with NP were selected, 13 of them allergic and 26 nonallergic. A control group of 11 individuals was also studied. The concentrations of interleukins were measured by enzyme-linked immunosorbent assay. RESULTS: There was a higher incidence of NP after the fourth decade of life and among men. The clinical symptoms were similar in both groups of patients with nasal polyposis and characterized by nasal obstruction and anosmia. A significant reduction of all interleukins studied was observed after surgical treatment. CONCLUSION: Levels of interleukins 1β, 3, 4, and 5 were significantly reduced after surgery and the levels of interleukins 1β and 5 were significantly lower in patients without recurrence of nasal polyposis after surgery when compared to those with recurrence.

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