Chicken Anaemia Virus in Egyptian Broiler; Clinical Signs and Molecular Characterization

SUMMARYSpecies of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained. Domestic canaries Serinus canaria were susceptible after inoculation of infected blood, and the long-lasting light parasitemia developed in two exposed birds. Clinical signs of illness were not reported. Illustrations of blood stages of P. matutinum (pLINN1) are given, and phylogenetic analysis identified the closely related avian Plasmodium species. The phylogeny based on partial cytochrome b (cyt b) sequences suggests that this parasite is most closely related to Plasmodium tejerai (cyt b lineage pSPMAG01), a common malaria parasite of American birds. Both these parasites belong to subgenus Haemamoeba, and their blood stages are similar morphologically, particularly due to marked vacuolization of the cytoplasm in growing erythrocytic meronts. Molecular data show that transmission of P. matutinum (pLINN1) occurs broadly in the Holarctic, and the parasite likely is of cosmopolitan distribution. Passeriform birds and Culex mosquitoes are common hosts. This study provides first molecular markers for detection of P. matutinum.

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Corrigendum to “Biological and molecular characterization of chicken anaemia virus isolates of Indian origin” [Virus Res. 118 (1/2) (2006) 78-86]

Virus Research ◽

10.1016/j.virusres.2007.04.008 ◽

2007 ◽

Vol 129 (1) ◽

pp. 71 ◽

Cited By ~ 1

Author(s):

Senthilkumar Natesan ◽

J.M. Kataria ◽

K. Dhama ◽

S. Rahul ◽

N. Bhardwaj

Keyword(s):

Molecular Characterization ◽

Chicken Anaemia Virus ◽

Indian Origin ◽

Virus Isolates

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Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil

Veterinary Dermatology ◽

10.1111/j.1365-3164.2010.00934.x ◽

2011 ◽

Vol 22 (3) ◽

pp. 249-256 ◽

Cited By ~ 10

Author(s):

Lissandro Gonçalves Conceição ◽

Livia Maria Rosa Acha ◽

Alexandre Secorun Borges ◽

Fernanda G. Assis ◽

Fabricia Hallack Loures ◽

...

Keyword(s):

Retrospective Study ◽

Molecular Characterization ◽

Clinical Signs

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Epidemiology and molecular characterization of chicken anaemia virus from commercial and native chickens in Taiwan

Transboundary and Emerging Diseases ◽

10.1111/tbed.12886 ◽

2018 ◽

Vol 65 (6) ◽

pp. 1493-1501 ◽

Cited By ~ 5

Author(s):

S.-C. Ou ◽

H.-L. Lin ◽

P.-C. Liu ◽

H.-J. Huang ◽

M.-S. Lee ◽

...

Keyword(s):

Molecular Characterization ◽

Chicken Anaemia Virus ◽

Native Chickens

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Recovery and molecular characterization of live Camelpox virus from skin 12 months after onset of clinical signs reveals possible mechanism of virus persistence in herds

Veterinary Microbiology ◽

10.1016/j.vetmic.2012.04.022 ◽

2012 ◽

Vol 159 (3-4) ◽

pp. 320-326 ◽

Cited By ~ 8

Author(s):

A.A. Yousif ◽

A.A. Al-Naeem

Keyword(s):

Molecular Characterization ◽

Clinical Signs ◽

Virus Persistence

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Isolation and Molecular Characterization of Aspergillus fumigatus and Aspergillus flavus Isolated from Poultry Birds in Ado- Ekiti, Nigeria

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2020/v6i230078 ◽

2020 ◽

pp. 31-44

Author(s):

E. D. Fagbohun ◽

K. J. Ayantola ◽

A. J. Toyin-Famoroti

Keyword(s):

Aspergillus Fumigatus ◽

Molecular Characterization ◽

Aspergillus Flavus ◽

Clinical Signs ◽

Signs And Symptoms ◽

Aspergillus Species ◽

Exercise Intolerance ◽

State University ◽

Poultry Farms

Aim: The study was carried out to isolate and identify Aspergillus species from commercial birds with suspected aspergillosis in the poultry farms within Ado Ekiti metropolis Nigeria. Place and Period of Study: The study was carried out in the Department of Microbiology, Faculty of Science, Ekiti State University Ado Ekiti, Nigeria in August 2016. Methodology: A total of 35 sick/suspected birds were collected randomly from three poultry farms. At Ago-Aduloju poultry farms, 15 samples were randomly collected from 1000 birds while at Ekiti State University poultry farms, 10 samples were randomly collected from 500 birds. At Federal Polytechnic Ado Ekiti poultry farms, 10 samples were randomly collected from 700 birds. The bird’s selection was on the basis of clinical signs and symptoms such as difficulty in breathing, weight loss, drooping of wings and exercise intolerance. Swab samples were collected from each suspected/sick bird for mycological culture and molecular characterization of the isolates from each bird was carried out. The isolates were identified based on the color of the culture on Potato Dextrose Agar and microscopic examination. Molecular identification was done using 23S Ribosomal RNA Gene and Partial Sequence. Results: Six fungal strains that showed similar morphological and cultural characteristics of Aspergillus species were isolated. The isolates were coded ASP 1, ASP 2, ASP 3, ASP 4, ASP 5, and ASP 6. The identified organisms were; Aspergillus fumigatus qH 107 (ASP 1), Aspergillus fumigatus qH 107 (ASP 2), Aspergillus flavus M09 (ASP 3), Aspergillus flavus UOMS6 (ASP 4), Aspergillus fumigatus qH 107 (ASP 5), Aspergillus flavus qH 107 (ASP 6). Conclusion: It is evident that Aspergillus species were predominant in poultry farms selected in this study. Necessary precaution should be put in place to prevent the spread of aspergillosis. Poultry farmers are advised to avoid damp environments, moldy feeds, dry and dusty litters. Adequate ventilation should always be provided in poultry farms to prevent Aspergillosis.

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Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals ◽

10.3390/ani11010105 ◽

2021 ◽

Vol 11 (1) ◽

pp. 105

Author(s):

Eduardo Reséndiz ◽

Helena Fernández-Sanz ◽

José Francisco Domínguez-Contreras ◽

Amelly Hyldaí Ramos-Díaz ◽

Agnese Mancini ◽

...

Keyword(s):

Molecular Characterization ◽

Molecular Analysis ◽

Baja California ◽

Clinical Signs ◽

Chelonia Mydas ◽

Baja California Sur ◽

Epidermal Hyperplasia ◽

Routine Monitoring ◽

Hematological Values

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

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Effects of chicken anaemia virus on experimental leukosis, induced by avian myelocytomatosis virus Mc29

BULGARIAN JOURNAL OF VETERINARY MEDICINE ◽

10.15547/bjvm.2272 ◽

2021 ◽

Vol 24 (2) ◽

pp. 229-238

Author(s):

K. B Simeonov ◽

A. I. Kril ◽

P. S. Dimitrov ◽

E. E. Shikova ◽

R. V. Russev

Keyword(s):

Bone Marrow ◽

Clinical Manifestation ◽

Virus Strain ◽

Clinical Course ◽

Clinical Signs ◽

Standard Protocol ◽

White Leghorn ◽

Chicken Anaemia Virus ◽

Hepatocellular Carcinomas ◽

Incidence And Mortality

The effects of concomitant infection with chicken anaemia virus (CAV) on the incidence, clinical manifestation and mortality from leukosis, induced by the avian myelocytomatosis virus strain Mc29 were studied. Experimental one-day-old 15 I line White Leghorn chickens were inoculated simultaneously with Mc29 and CAV or with Mc29 alone and observed daily for clinical signs and mortality. Both groups of chickens inoculated with Mc29 virus strain alone or in combination with CAV developed tumours and died within 57 days. Necropsy has been performed on all dead birds following the standard protocol. Organ samples from thymuses, spleens, bone marrow, and livers were collected and histopathologically investigated. Neoplasms detected included myelocytomas, nephroblastomas and hepatocellular carcinomas. In addition, 50% of the CAV/Mc29-inoculated chickens developed epithelial type thymomas. However, no such lesions were found in chickens infected with Mc29 alone. No significant differences in the clinical course of leukosis between the two experimental groups of chickens were observed. The results indicated that CAV infection did not affect substantially the incidence and mortality from avian leukosis, induced by myelocytomatosis virus strain Mc29, but contributed to greater variety of the induced tumours.

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Detection and Molecular Characterization of Rotavirus and Picobirnavirus in Wild Avians from Amazon Forest

10.1101/2020.09.15.297689 ◽

2020 ◽

Author(s):

José Wandilson Barboza Duarte Júnior ◽

Elaine Hellen Nunes Chagas ◽

Ana Carolina Silva Serra ◽

Lizandra Caroline dos Santos Souto ◽

Edvaldo Tavares da Penha Júnior ◽

...

Keyword(s):

Molecular Characterization ◽

Clinical Signs ◽

Wild Birds ◽

Interspecies Transmission ◽

Amazon Forest ◽

Rotavirus A ◽

Exotic Birds ◽

Veterinary Hospital ◽

Nsp3 Gene

ABSTRACTThe present study reports the detection and molecular characterization of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG) and picobirnavirus (PBV) in fecal specimens of wild and exotic birds (n = 23) from different cities of Pará state, which were hospitalized at Veterinary Hospital of the Federal University of Pará, Brazil, between January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, equine-like G3 and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in 1 of the 23 samples tested and was classified in the Genogroup I. The findings represent the first report of the circulation of RVA, RVD, RVF, RVG and PBV genotypes in wild birds in Brazil and suggest the possible interspecies transmission of RVs and PBVs.

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Molecular Characterization of Muscle Infecting Myxobolus sp. Causing Outbreak in Labeo rohita, Rohu: First Report from Andaman Islands

Indian Journal of Animal Research ◽

10.18805/ijar.b-3931 ◽

2020 ◽

Author(s):

K Saravanan ◽

Shailesh Kumar ◽

J Praveenraj ◽

B L Meena ◽

B L Kasinath

Keyword(s):

Molecular Characterization ◽

Clinical Signs ◽

The Body ◽

Andaman And Nicobar Islands ◽

Phylogenetic Tree Analysis ◽

Spinal Curvatures ◽

First Report ◽

Freshwater Aquaculture ◽

Nicobar Islands ◽

Loss Of Appetite

Among the myxozoan parasites, the genus Myxobolus is considered as the emerging parasite in freshwater aquaculture. In the present study, a disease outbreak associated with mass mortality was investigated in a carp farm located at North and Middle Andaman district of Andaman and Nicobar Islands. The affected rohu fry exhibited the clinical signs such as lethargic movement, dark body colouration, loss of appetite, excess mucus secretion, 1-2 mm multifocal white nodules on the body surface and skeletal deformities including spinal curvatures. Molecular characterization using 18S rRNA along with clinical signs identified the causative agent as muscle infecting Myxobolus sp. (GenBank accession number MK128509) which showed 95.45% identity with Myxobolus musculi and 95.23% identity with Myxobolus pseudodispar. Genetic distance and phylogenetic tree analysis were performed to elucidate the relationship between the Myxobolus sp. obtained in the present study and the members of other congeners. This investigation serves as the first report of Myxobolus sp. outbreak from Andaman and Nicobar Islands and also reiterates the need for implementation of strict biosecurity measures to preserve the freshwater aquatic fauna of these Islands.

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