Germinability and Seedling Growth Performance of Chilli (Capsicum annuum) Seeds in Response to Different Gibberellic Acid Concentrations Pre-Treatment

2021 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Ryn Brenda Junaidy ◽  
Shafeeqa Shahruddin
Keyword(s):  
Gibberellic Acid ◽  
Growth Performance ◽  
Seedling Growth ◽  
Crop Production ◽  
Germination Rate ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Seedling Vigour ◽  
Seed Germinability ◽  
Significant Difference

Chilli seeds are always associated with physiological dormancy characteristics or endogenous dormancy that has affected their germination potential. Problems noticed on the low seed germination rate, vigour growth, further may lead to the poor seedling growth pattern of chilli crop production. Gibberellic Acid (GA3) which is usually used as a treatment solution is not a new advent of seed dormancy breaking methods for chilli. But, looking forwards to a better GA3 concentration is still needed for the discovery of this treatment potential effect on chilli seed germinability and seedling growth. Thus, this study aimed to evaluate the effect of different GA3 concentrations on seed emergence and seedling growth performance in chilli. In this experiment, chilli seeds were imbibed with 25 mg/L, 75 mg/L and 125 mg/L concentration of GA3 with three replications each; for 24 hours at room temperature (32±4°C) and the untreated seeds as a control. The data collection on final germination percentage was taken daily for 21 days after sowing. While the 15 germinated seeds were directly planted on the 5 polybags arranged in the net house of Junaidy Jonik Farm, Sabah, Malaysia. The experiment was arranged in a completely randomized design with three replications. Data were subjected to analysis of variance with SAS version 9.4 and the significant means were separated by the least significant difference test at P<0.05. Significant differences were observed in the seed germinability measured; germination rate index (GRI), mean germination time (MGT) and final germination percentage (FGP), as well as on seedling vigour index (SVI) between treated and control seeds. Conversely, plant height, number of internodes, number of leaves and fresh weight of seedlings showed no significant differences among treatments. It was concluded that the use of GA3 was able to enhance chilli seeds germinability and could display a better SVI than the control. It is recommended to use GA3 treatment at 25 mg/L of concentration, as it may give an advantage to both economic and biological importance in producing higher germinability and seedling growth performance in chilli.

scholarly journals Effect of Soaking Treatment on Germination of Hard Coated Tropical Forest Tree Seeds

2021 ◽  
Vol 19 (2) ◽  
pp. 1-9
Author(s):  
Juventine Boaz Odoi ◽  
David Mugeni ◽  
Robert Kiiza ◽  
Betty Apolot ◽  
Samson Gwali
Keyword(s):  
Seed Germination ◽  
Growth Performance ◽  
Seedling Growth ◽  
Cold Water ◽  
Block Design ◽  
Forest Tree ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Terminalia Catappa ◽  
Maesopsis Eminii

Seed germination and seedling growth performance of Maesopsis eminii and Terminalia catappa under different water soaking treatments were evaluated for 120 days under nursery conditions. A total of 1400 seeds were pre-treated with hot (95oC) and cold water (ambient temperature) by soaking for 12, 24 and 48 hours with a control of no soaking. The seeds were sown directly into polythene pots filled with uniform growth medium (top forest soil, sand and clay soilmixed in a ratio of 5:3:2) to avoid disturbance of the root system after germination. The seeds were sown in a randomized block design with seven treatments and three replicates. Data were analysed using ANOVA in GenStat v18. Results indicated that soaking enhanced seed germination. Soaking of seeds in cold water for 12 hours resulted into higher germination (90% for Terminalia catappa and 85% for Maesopsis eminii) than the control (48%). Soaking period and water temperature significantly influenced seedling vigour (F value = 0.962; p = 0.038). Soaking seeds in cold water for 24 hours enhanced Maesopsis eminii seedling growth by 8.0 cm Terminalia catappa seedlings by 7.4 cm. Seed dormancy, germination percentage and growth performance in hard coated seeds such as Maesopsis eminii and Terminalia catappa can be broken by soaking in cold water for 12-24 hours. Pre-germination treatments significantly influences the germination and seedling growth. Keywords: Maesopsis eminii, Terminalia catappa, seeds, pre-germination, soaking.

scholarly journals Effect of desiccation and salinity stress on seed germination and initial plant growth of Cucumis melo

2013 ◽  
Vol 31 (4) ◽  
pp. 833-841 ◽  
Author(s):  
S. Sohrabikertabad ◽  
A. Ghanbari ◽  
H.R. Mohassel, Mohamad ◽  
M.N. Mahalati ◽  
J. Gherekhloo
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Cucumis Melo ◽  
Germination Rate ◽  
Germination Percentage ◽  
Sodium Content ◽  
Invasive Weed ◽  
The North ◽  
Root And Shoot Length ◽  
Significant Difference

Smellmelon, an annual invasive weed of soybean production fields in the north of Iran, reproduces and spreads predominately through seed production. This makes seed bank survival and successful germination essential steps in the invasive process. To evaluate the potential of Smellmelon to invade water-stressed environments, laboratory studies were conducted to investigate the effect of desiccation and salinity at different temperatures on seed germination and seedling growth of Cucumis melo. Seeds were incubated at 25, 30, 35 and 40 ºC in the darkness in a solution (0, -0.2, -0.4, -0.6, -0.8, 1 and 1.2 MPa) of a salt (NaCl), and in a solution (0, -2, -4, -6, -8, -10, -12 bar) of PEG-6000 (Polyethylene glycol), in two separate experiments. The results showed that the highest percentage and rate of germination occurred at 35 ºC in salt concentrations of 0, -0.2, -0.4 MPa and PEG concentrations of 0, -2, -4 bar. Increasing the concentration of salt (NaCl) and PEG limited germination, seedling growth and water uptake but increased the sodium content in the seedlings. No significant difference was observed among 0, -0.2 and -0.4 MPa of NaCl and among 0, -2 and -4 bar of PEG concentration at 35 ºC. The negative effects of PEG were more than those of NaCl on germination percentage and germination rate. Increased stress levels lead to reduction of root and shoot length, and SVL of seedlings. Na+ content of seedling decreased with limited seedling growth of C. melo.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals GIBBERELLIC ACID DURING PRIMING OF PURPLE CONEFLOWER [ECHINACEA PURPUREA (L) MOENCH.] SEEDS IMPROVES GERMINATION AND SEEDLING EMERGENCE

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 186c-186
Author(s):  
Janine O. Haynes ◽  
Wallace G. Pill
Keyword(s):  
Gibberellic Acid ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Purple Coneflower ◽  
Percentage Points

Purple coneflower seeds following priming (-0.04 MPa, 10 days, 15C, darkness) osmotically in polyethylene glycol 8000 (PEG) or matrically in expanded no. 5 vermiculite had greater germination rate and synchrony at continuous 20C or 30C than untreated seeds, but germination percentage was unaffected. Inclusion of 5.5 × 10-2 M gibberellic acid (GA3 as ProGibb Plus 2X, Abbott Laboratories, N. Chicago, Ill.) further improved germination rate and synchrony at 20C, but not at 30C. In a greenhouse study (30C day/27C night, July-August natural light), seeds primed in PEG or vermiculite containing G A3 compared to untreated seeds had 6 percentage points higher maximum emergence (ME), 3.3 fewer days to 50% ME, 1.9 fewer days between 10% and 90% ME, 116% greater shoot dry weight, and 125% longer leaves at 16 days after planting in peat-lite. Inclusion of ethephon (0.01 m, as Florel) either alone or with GA3 during priming provided no benefit to seed germination or seedling emergence. Moistened vermiculite substituted for PEG solution as a priming medium for purple coneflower seeds, the priming benefit on seedling emergence and growth being enhanced by 5.5 × 10-2m G A3 inclusion in the priming media.

scholarly journals Germination and Seedling Growth of Desmanthus illinoensis and Desmodium canadense in Response to Mechanical Scarification

HortScience ◽  
2010 ◽  
Vol 45 (10) ◽  
pp. 1554-1558 ◽  
Author(s):  
Michael W. Olszewski ◽  
Courtney A. Young ◽  
Joel B. Sheffield
Keyword(s):  
Electrical Conductivity ◽  
North America ◽  
Seedling Growth ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Physical Damage ◽  
Desmanthus Illinoensis ◽  
Illinois Bundleflower

Illinois bundleflower [Desmanthus illinoensis (Michx.) MacMill. ex B.L. Rob. & Fernald] and showy ticktrefoil [Desmodium canadense (L.) DC.] are legumes native to North America used during meadow restoration efforts. However, insufficient or slow germination or reduced emergence may result attributable to seedcoat-mediated reductions in permeability to water. The objective of this research was to determine the effectiveness of a single-speed electric scarifier lined with 40-grit sandpaper for increasing germination and seedling growth of two native legumes. Seeds of Illinois bundleflower and showy ticktrefoil were mechanically scarified for 3, 6, or 12 s before they were subjected to germination and vigor testing. After scarification, final germination percentage (FGP), germination rate, and uniformity at multiple temperatures (15, 20, and/or 20 to 30 °C) were improved for Illinois bundleflower. However, FGP decreased for showy ticktrefoil, whereas germination rate and uniformity increased. For both species, there was a decline in FGP with longer scarification durations. Illinois bundleflower seed subjected to scarification and accelerated aging (AA) had higher FGP than non-scarified seed subjected to AA (59% and 6%, respectively), whereas both scarified and non-scarified seed of showy ticktrefoil subjected to AA had low FGP (11% and 18%, respectively). Mechanical scarification increased electrical conductivity (EC) of leachates for both species, but scarified showy ticktrefoil seed subjected to AA resulted in the highest EC compared with all other treatments, indicating a reduction of vigor. Evaluation of a seedling grow-out test 3 weeks after sowing confirmed that emergence was enhanced after 3 s of mechanical scarification of Illinois bundleflower seed but that scarification of showy ticktrefoil seed decreased emergence and increased the number of abnormal seedlings. Mechanical scarification resulted in peripheral damage and seed tissue obliteration of both species as indicated by viewing with a stereomicroscope. We conclude that scarifier limitations caused excessive physical damage of showy ticktrefoil. For Illinois bundleflower, however, mechanical scarification using an electric scarifier increased emergence from 18% (non-scarified seed) to 77% after 3 s of scarification and FGP from 32% (non-scarified seed) to 87% after 3 s of scarification. Compared with responses from non-scarified Illinois bundleflower seeds, scarification treatment also resulted in 24% faster germination and 37% more uniformity.

scholarly journals The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Tomato Seeds ◽  
Germination Characteristics ◽  
Salinity Levels ◽  
Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

Impact of Seed Maturation and Drought on Seed Germination and Early Seedling Growth in White Clover (Trifolium repens L.)

2021 ◽  
Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  
Keyword(s):  
Seedling Growth ◽  
White Clover ◽  
Germination Percentage ◽  
Seed Maturation ◽  
Percentage Germination ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling ◽  
The Mean

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.

scholarly journals Utilization of Grape Fruit Waste-based Substrates for Seed Germination and Seedling Growth of Lemon Basil

2017 ◽  
Vol 27 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Mohammed El-Sayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Salah El-Hendawy
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Low Cost ◽  
Germination Rate ◽  
Germination Percentage ◽  
Waste Recycling ◽  
Grape Fruit ◽  
Fruit Waste ◽  
One To One

Grape (Vitis vinifera) waste management is a major problem in juice production, but it could be transformed into a major opportunity if the waste was recycled and used as a nursery growing medium. The aim of this study was to evaluate the suitability of four composts based on squeezed grape fruit waste (SGFW), mixed with coir or vermiculite in a one-to-one ratio by volume to form 13 growing media, for seed germination and seedling growth of ‘Mrs. Burns’ lemon basil (Ocimum basilicum var. citriodora). The final germination percentage (FGP), corrected germination rate index (CGRI), survival percentage, and seedling growth of ‘Mrs. Burns’ lemon basil were the variables measured. Pure SGFW reduced seed germination and seedling growth. The medium combining pure SGFW with vermiculite in a one-to-one ratio by volume was optimal for seed germination and seedling growth; in this medium the highest FGP, CGRI, survival rate, and growth parameters were recorded. The negative effects of pure SGFW composts were eliminated by mixing all composts with coir or vermiculite. These waste recycling media are low-cost products that can be beneficially used in nurseries on a commercial scale.

Application of the hydrotime model to assess seed priming effects on the germination of rapeseed (Brassica napus L.) in response to water stress

Botany ◽  
2020 ◽  
Vol 98 (5) ◽  
pp. 283-291
Author(s):  
Samaneh Tatari ◽  
Farshid Ghaderi-Far ◽  
Ahad Yamchi ◽  
Asieh Siahmarguee ◽  
Ali Shayanfar ◽  
...  
Keyword(s):  
Water Stress ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
The Other ◽  
Dormancy Breaking ◽  
Brassica Napus L ◽  
Hydrotime Model ◽  
Response To Water ◽  
Base Water Potential

Our aim was to determine whether the parameters of the hydrotime model can be used to compare the effects of seed priming treatments on germination. Seeds of three rapeseed cultivars (‘H61’, ‘H4815’, and ‘DK003’) were exposed to priming treatments and then their germination was tested at different water potentials. Seed priming improved the germination percentage, which was higher in ‘H4815’ than in the other two cultivars, and it increased germination rate (decreased θH) and uniformity (decreased σψb). The increase in germination rate was higher in PEG-osmoprimed than in NaCl-osmoprimed and hydroprimed seeds. The base water potential allowing 50% germination ([Formula: see text]) also was affected by seed priming, but the response of cultivars to different priming treatments varied. Compared with the control seeds, hydropriming of ‘H61’ seeds shifted ψb(50) toward negative values, whereas NaCl-osmopriming of ‘H4815’ seeds shifted ψb(50) toward negative values. On the other hand, priming of ‘DK003’ seeds shifted ψb(50) to more positive values and increased susceptibility to water stress. After dormancy-breaking and dormancy-induction treatments, ψb(50) shifted to more negative or positive values, indicating the release of primary dormancy and induction of dormancy, respectively. Therefore, priming treatments should be selected, depending on the environmental conditions anticipated to prevail during the germination of rapeseed seeds.

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