scholarly journals Effect of Soaking Treatment on Germination of Hard Coated Tropical Forest Tree Seeds

2021 ◽  
Vol 19 (2) ◽  
pp. 1-9
Author(s):  
Juventine Boaz Odoi ◽  
David Mugeni ◽  
Robert Kiiza ◽  
Betty Apolot ◽  
Samson Gwali

Seed germination and seedling growth performance of Maesopsis eminii and Terminalia catappa under different water soaking treatments were evaluated for 120 days under nursery conditions. A total of 1400 seeds were pre-treated with hot (95oC) and cold water (ambient temperature) by soaking for 12, 24 and 48 hours with a control of no soaking. The seeds were sown directly into polythene pots filled with uniform growth medium (top forest soil, sand and clay soilmixed in a ratio of 5:3:2) to avoid disturbance of the root system after germination. The seeds were sown in a randomized block design with seven treatments and three replicates. Data were analysed using ANOVA in GenStat v18. Results indicated that soaking enhanced seed germination. Soaking of seeds in cold water for 12 hours resulted into higher germination (90% for Terminalia catappa and 85% for Maesopsis eminii) than the control (48%). Soaking period and water temperature significantly influenced seedling vigour (F value = 0.962; p = 0.038). Soaking seeds in cold water for 24 hours enhanced Maesopsis eminii seedling growth by 8.0 cm Terminalia catappa seedlings by 7.4 cm. Seed dormancy, germination percentage and growth performance in hard coated seeds such as Maesopsis eminii and Terminalia catappa can be broken by soaking in cold water for 12-24 hours. Pre-germination treatments significantly influences the germination and seedling growth. Keywords: Maesopsis eminii, Terminalia catappa, seeds, pre-germination, soaking.

2021 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Ryn Brenda Junaidy ◽  
Shafeeqa Shahruddin

Chilli seeds are always associated with physiological dormancy characteristics or endogenous dormancy that has affected their germination potential. Problems noticed on the low seed germination rate, vigour growth, further may lead to the poor seedling growth pattern of chilli crop production. Gibberellic Acid (GA3) which is usually used as a treatment solution is not a new advent of seed dormancy breaking methods for chilli. But, looking forwards to a better GA3 concentration is still needed for the discovery of this treatment potential effect on chilli seed germinability and seedling growth. Thus, this study aimed to evaluate the effect of different GA3 concentrations on seed emergence and seedling growth performance in chilli. In this experiment, chilli seeds were imbibed with 25 mg/L, 75 mg/L and 125 mg/L concentration of GA3 with three replications each; for 24 hours at room temperature (32±4°C) and the untreated seeds as a control. The data collection on final germination percentage was taken daily for 21 days after sowing. While the 15 germinated seeds were directly planted on the 5 polybags arranged in the net house of Junaidy Jonik Farm, Sabah, Malaysia. The experiment was arranged in a completely randomized design with three replications. Data were subjected to analysis of variance with SAS version 9.4 and the significant means were separated by the least significant difference test at P<0.05. Significant differences were observed in the seed germinability measured; germination rate index (GRI), mean germination time (MGT) and final germination percentage (FGP), as well as on seedling vigour index (SVI) between treated and control seeds. Conversely, plant height, number of internodes, number of leaves and fresh weight of seedlings showed no significant differences among treatments. It was concluded that the use of GA3 was able to enhance chilli seeds germinability and could display a better SVI than the control. It is recommended to use GA3 treatment at 25 mg/L of concentration, as it may give an advantage to both economic and biological importance in producing higher germinability and seedling growth performance in chilli.


2021 ◽  
Vol 56 ◽  
pp. 113-127
Author(s):  
M. Zahidul Islam Hawlader TUHIN ◽  
◽  
Taufika Islam ANEE ◽  
Naznin AHMED ◽  
Mira RAHMAN ◽  
...  

An experiment was conducted to investigate the effect of different levels of cadmium (Cd) stress on germination, morphology, physiology and yield of rice. There were five treatments, viz. control (no Cd), 0.5 mM CdCl2, 1 mM CdCl2, 1.5 mM CdCl2 and 2 mM CdCl2, carried out by a randomized complete block design (RCBD) with three replications. Cadmium stress caused a significant reduction of plant height and tillers hill-1 in a dose-dependent manner. The highest decline of relative water content and SPAD value was found at 1.5 mM (11 and 16%) and 2 mM (14 and 19%) Cd stress compared to the control. The number of effective tillers hill-1, filled grains panicle-1, 1000-grain weight, length of panicle, grain yield and straw yield reduced significantly by 44, 32, 15, 24, 37 and 45%, respectively, over the control. Germination and seedling-related parameters were recorded 10 days after germination. The lowest germination percentage (27%), germination index (27%) and seedling vigour index (49%) were observed at the highest level of Cd stress in comparison to the control. Cadmium stress resulted in maximum abnormal seedlings and markedly reduced length of root and shoot as well as their fresh and dry weight. Root shoot ratio showed differential responses to Cd stress. Thus, it can be concluded that a higher level of Cd in soil has a detrimental effect on seed germination, morphology, physiology and yield of rice.


2019 ◽  
Vol 17 (3) ◽  
pp. 281-287
Author(s):  
Shaila Shermin Tania ◽  
Md. Mokter Hossain ◽  
M Abul Hossain

The experiment was conducted to find out the effects of hydropriming on seed germination, seedling growth and yield of bitter. Two single factor experiments were conduced at the laboratory and field of Seed Science and Technology Department following completely randomized design with three replications and randomized complete block design with five replications, rspectively. The experiments comprised six soaking treatments viz. T0: untreated (control), T1: tap water (around 25º ± 2º C) for overnight, T2:cold water (12ºC) for 5 min, T3: cold water (12ºC) for 10min, T4:cold water (12ºC) for 15min, T5: hot water (45ºC) for 5 min and T6: hot water (45ºC) for 10min soaking. The highest germination (88.0%) was obtained from T5 treatment and the lowest germination  (30.0%) was recorded in control treatment. It was also observed that T5 treatment exehibited superior performances in respect of most of the parameters studied in the laboratory as well as in fiield experiment as compeared to control treatment. In respect of fruit yield per plant, maximum number of fruits obtained from T5 treatment (16 fruits/plant) followed by T6 (14 fruit/plant), T1 (12 fruit/plant), T2 (12.0 fruit/plant), T3 (11.2 fruit/plant) and T4 (11.2 fruit/plant) and the minimum number of fruits per plant recorded from the control treatment (7.6). From the findings of these study it can be concluded that bitter gourd seeds soaked in hot water at 45ºC for 5 min could improve seed germination, expedite seedling growth and eventually increase the yield of fruit. J Bangladesh Agril Univ 17(3): 281–287, 2019


2018 ◽  
Vol 25 (1) ◽  
pp. 57-62
Author(s):  
Dharam Chand Attri ◽  
◽  
Mohan Chandra Nautiyal ◽  
Vijay Laxmi Trivedi ◽  
Jyoti Sati ◽  
...  

An experimental trial was conducted to access the impact of cold stratification and different growth media on seed germination and seedling growth performance of Picrorhiza kurrooa under ex-situ conditions at lower altitude. The experiment was carried out in CRD (Completely Randomized Design) with 20 treatments, three replications and 60 seeds per treatment. Influence of growth media on seed germination was measured by thirteen parameters. Parameters such as minimum and maximum days required for initial (8.00±1.00) and final (28.67±2.08) germination was observed in T10 (Soil+Sand+Azotobacter+Cocopeat) and T18 (Soil+FYM+Cocopeat+Rockphosphate+Vermicomposte). The maximum germination percentage (83.33±8.82%), plant height (2.50±0.10) and number of shoots (3.67±0.58) were recorded in T5 (Soil+Sand+FYM+Moss). The maximum number of leaves (9.00±3.46), leaf length (1.53±0.35), leaf width (0.93±0.15), shoot length (2.70±0.26), number of roots (4.33±2.31) and root length (2.63±0.15) was found significantly superior in T18 (Soil+FYM+Cocopeat+Rockphosphate +Vermicomposte).


2019 ◽  
pp. 25-38
Author(s):  
M. A. Dorri ◽  
B. Kamkar ◽  
M. Aghdasi ◽  
A. R. Safahani

Milk thistle (Silybum marianum) is used as a medicinal plant in the treatment of liver diseases. In order to investigate germination and seedling growth in S. marianum subjected to NaCl, a three replicated experiment was carried out in a randomized complete block design in the laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included seven salinity levels (50, 75, 100, 125, 150, 175 and 200 mM) and a control (distilled water). Germination percentage (GP), mean germination time (MGT), seed-ling vigour index (SVI), shoot, root and seedling weight were measured. The effect of salinity levels was significant on GP, SVI, MGT and seedling weight and length (p ≤ 0.01). However, the NaCl concentration effect was not significant on shoot : root length ratio and or shoot : root weight ratios. Results showed that germination decreased when salinity increased, while MGT increased. MGT was 1.75 times higher than in the control at the highest salt concentration. MGT difference was not significant between 75 mM NaCl and con-trol, while it dramatically increased by increasing the NaCl concentration from 150 to 200 mM NaCl. Reduc-tion slope and salt tolerance index (STI) were estimated for germination (0.54 and 231.9) and seedling stage (0.24 and 237.4). According to the results, milk thistle could be considered as a valuable medicinal plant in fairly salinized areas.


2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Eric B. Kouam ◽  
Simon M. Ndo ◽  
Marie S. Mandou ◽  
Asafor H. Chotangui ◽  
Christopher M. Tankou

AbstractThis study was carried out to determine effects of salinity on germination and seedling development of commercially important common bean genotypes in Cameroon. The seeds of eight genotypes were used for both trials. The germination experiments were carried out on petri dishes in the laboratory while early growth trials continued in plastic pots in the screenhouse. Both stages were setup using a randomized complete block design with three replications. Germination and early growth trials of the different genotypes were studied using distilled water as control or osmotic potentials of 50, 100, 150 and 200mM NaCl to study the effects of salinity on germination and seedling growth characteristics. Leaf concentrations of Na+ and K+ were determined. At the germination level, germination percentage, germination index and the coefficient of velocity of germination decreased with increasing salinity while the mean germination time and time to 50% germination increased with increasing level of salt. All the growth variables decreased with increasing salinity with the exception of the root length which was not affected by salinity. It was observed that increasing salinity induced a significant increase in leaf Na+ and substantial reduction in the accumulation of K+ in the leaves. For ions accumulation, germination variables and growth parameters, significant differences at 0.001 probability levels were found among salinity treatments, common bean genotypes and most of their interactions. Significant correlations were found between all germination variables and between most growth parameters. From the effect of salt application, the common bean genotypes namely KEBCB049, KEB-CB053 and Mac-33 were the most tolerant while KEB-CB055 and KEB-CB050 were the most sensitive. The results confirm that there is genotypic variation in salinity tolerance and that the most tolerant genotypes should be further explored in selection programs, with the aim that they should be promoted for cultivation in tropical zones affected by salinity.


2010 ◽  
Vol 62 (10) ◽  
pp. 2459-2466 ◽  
Author(s):  
Han Peng ◽  
Wu Geng ◽  
Wu Yong-quan ◽  
Li Mao-teng ◽  
Xiang Jun ◽  
...  

In this paper, we report the effects of heavy metals (HMs) (cadmium and mercury) on seed germination and seedling growth of Phragmites australis and Triarrhena sacchariflora, which are the two main typical emerging plants in Hongze Lake wetland. The results showed that there was a reduction in germination percentage, germination index and seedling length as HM concentration in the growing media increased for both treatments. The effect of HMs toxicity on seed germination and seedling growth of T. sacchariflora was more obvious than of P. australis. At the stage of seed germination, P. australis and T. sacchariflora were sensitive to Hg2 +  and Cd2 + , respectively, and Hg2 +  was more toxic than Cd2 +  at the stage of seedling growth. The effect of HMs toxicity is not invariable during plant growth. Compared to the stage of seedling growth, P. australis and T. sacchariflora are more susceptible to HMs at the stage of seed germination. In addition, we calculated the ecological thresholds of P. australis to Cd and Hg are 19.32 and 1.08 mg kg−1, and that of T. sacchariflora to Cd is 4.62 mg kg−1 based on the lab simulation. The results also indicated that the species of P. australis is more tolerant than T. sacchariflora to the HMs and is a better candidate for restoration in Hongze Lake wetland ecosystem.


Weed Science ◽  
1984 ◽  
Vol 32 (6) ◽  
pp. 781-785 ◽  
Author(s):  
Megh Singh ◽  
Nagi Reddy Achhireddy

The germination of milkweedvine (Morrenia odorataLindl. ♯3MONOD) seed at 20 or 25 C was unaffected by a 12-h photoperiod. The 12-h photoperiod, however, decreased germination by 50% at 15 C. No germination occurred at 35 C regardless of photoperiod. By alternating 35 C for 12 h with 20 C for 12 h, the germination percentage was 57%. Seedling growth was maximum at alternating temperatures of 30/20 C. Optimum pH for germination and seedling growth was 7 and germination did not occur at pH levels below 6. Seed germination declined steadily at osmotic stress below −0.12 MPa; no germination occurred at −0.5 MPa. Seedling growth was not influenced by osmotic stress down to −0.18 MPa. Germination percentages of seeds kept under aerated water and nonaerated water were similar, but the seedling growth was greater in aerated water. Seedling emergence was maximum from depths of 0.5 to 2.5 cm, but no seedling emerged from 0 or 10 cm. Planting depth was negatively correlated (r = −0.7) with shoot growth but positively correlated (r = +0.98) to root growth.


Weed Science ◽  
1984 ◽  
Vol 32 (6) ◽  
pp. 757-761 ◽  
Author(s):  
Nagi Reddy Achhireddy ◽  
Megh Singh

Allelopathic effects of lantana (Lantana camaraL. ♯3LANCA) residues (root, shoot), foliar leachates, and the soil (where lantana was grown) on milkweedvine (Morrenia odorataLindl. ♯3MONOD) seed germination and growth over a 30-day period were examined. Foliar leachates or the soil collected from the field where lantana had been growing had no effect on the final germination percentage or the seedling growth of milkweedvine. Incorporation of dried lantana shoot or root material into soil had no effect on the final percentage germination but caused significant reductions in milkweedvine growth over a 30-day test period. Roots were more inhibitory than shoots. Fifty percent of milkweedvine seedlings died within 15 days after germination at 1% (w/w) dried lantana root incorporation into the soil, and higher concentrations increased seedling death. Lantana roots incorporated into the soil produced foliar symptoms such as wilting and desiccation, whereas lantana shoots incorporated into the soil produced yellowing of the foliage of milkweedvine. Allelopathic activity of lantana residues was still strong even after decomposition of lantana residues for 4 weeks prior to the planting of milkweedvine seeds.


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