scholarly journals Development of aerobic granules in sequencing batch reactor with p-nitrophenol as sole carbon source

2012 ◽  
Vol 2 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Farrukh Basheer ◽  
I. H. Farooqi
Keyword(s):  
Removal Efficiency ◽  
Degradation Rate ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Substrate Inhibition ◽  
Gas Chromatography Mass Spectrometry ◽  
Aerobic Granules ◽  
Compact Structure ◽  
Inhibitory Compounds ◽  
Haldane Model

The present study was aimed at the development of aerobic granules in sequencing batch reactor (SBR) for the biodegradation of p-nitrophenol (PNP). The reactor was started with 50 mg L−1 of PNP. Aerobic granules first appeared within 1 month of the start up of the reactor. The granules were large and strong and had a compact structure. The diameter of stable granules on day 200 was in the range of 2–3 mm. The integrity coefficient and granule density was found to be 98% and 1,031 kg m−3 respectively. The settling velocity of granules was found to be in the range of 3 × 10−2 to 4 × 10−2m s−1. The aerobic granules were able to degrade PNP up to 700 mg L−1 at a removal efficiency of 87%. Gas chromatography–mass spectrometry studies confirmed that the biodegradation of PNP occurred by an initial oxygenase attack that resulted in the release of nitrite and the accumulation of hydroquinone. The specific PNP degradation rate in aerobic granules followed the Haldane model for substrate inhibition. A high specific PNP degradation rate up to 0.872 g PNP g−1 VSS−1 d−1 was sustained up to PNP concentration of 200 mg L−1. Higher removal efficiency, good settling characteristics of aerobic granules, makes a SBR suitable for enhancing the microorganism potential for biodegradation of inhibitory compounds.

Biodegradation of Paracetamol by Aerobic Granules in a Sequencing Batch Reactor (SBR)

2012 ◽  
Vol 441 ◽  
pp. 531-535 ◽  
Author(s):  
Jun Hu ◽  
Lan Zhou ◽  
Qing Wei Zhou ◽  
Fang Wei ◽  
Li Li Zhang ◽  
...  
Keyword(s):  
Degradation Rate ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Substrate Inhibition ◽  
Energy Resource ◽  
Granular Sludge ◽  
Aerobic Granules ◽  
Dominant Form ◽  
Start Up ◽  
Insight Into

Aerobic granules efficient at degrading paracetamol as the sole carbon and energy resource were successfully developed in a sequencing batch reactor (SBR). Aerobic granules were first observed about 220 days after reactor start-up. The images SEM showed the aerobic granules typically consisted of coccus and bacillus. Meanwhile, the size distribution of aerobic granules was analyzed on day 200. The result indicated that the granules eventually grew to become the dominant form of biomass in the reactor. The granular sludge on day 80 and 200 degraded paracetamol completely in 48 h and 28 h, respectively, indicating that granulation contributed to paracetamol degradation. The specific paracetamol degradation rate was observed to increase with increasing paracetamol initial concentration from 500 to 5000 mg/L, peaked at 1200 mg-MTBE/g-VSS·h, and declined with further increases in MTBE concentration as substrate inhibition effects became significant. This study demonstrates that paracetamol can be effectively degraded by aerobic granules and gives insight into the microorganisms potentially involved in the process.

Biodegradation of o-nitrophenol by aerobic granules with glucose as co-substrate

2012 ◽  
Vol 65 (12) ◽  
pp. 2132-2139 ◽  
Author(s):  
Farrukh Basheer ◽  
M. H. Isa ◽  
I. H. Farooqi
Keyword(s):  
Activated Sludge ◽  
Kinetic Parameters ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Aerobic Granules ◽  
Haldane Model

Aerobic granules to treat wastewater containing o-nitrophenol were successfully developed in a sequencing batch reactor (SBR) using activated sludge as inoculum. Stable aerobic granules were obtained with a clearly defined shape and diameters ranging from 2 to 6 mm after 122 days of operation. The integrity coefficient (IC) and granules density was found to be 98% and 1,054 kg m−3 respectively. After development of aerobic granules, o-nitrophenols were successfully degraded to an efficiency of 78% at a concentration of 70 mg L−1. GC-MS study revealed that the biodegradation of o-nitrophenol occured via catechol via nitrobenzene pathway. Specific o-nitrophenol biodegradation rates followed the Haldane model and the associated kinetic parameters were found as follows: Vmax = 3.96 g o-nitrophenol g−1VSS−1d−1, Ks = 198.12 mg L−1, and Ki = 31.16 mg L−1. The aerobic granules proved to be a feasible and effective way to degrade o-nitrophenol containing wastewater.

Characterization of aerobic granules formed in an aspartic acid fed sequencing batch reactor under unfavorable hydrodynamic selection conditions

Chemosphere ◽  
2020 ◽  
Vol 260 ◽  
pp. 127600
Author(s):  
Bin-Bin Wang ◽  
Qin Luo ◽  
Hui-Juan Li ◽  
Qian Yao ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Aerobic Granules

scholarly journals Bacterial Diversity and Function of Aerobic Granules Engineered in a Sequencing Batch Reactor for Phenol Degradation

2004 ◽  
Vol 70 (11) ◽  
pp. 6767-6775 ◽  
Author(s):  
He-Long Jiang ◽  
Joo-Hwa Tay ◽  
Abdul Majid Maszenan ◽  
Stephen Tiong-Lee Tay
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Bacterial Diversity ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Phenol Degradation ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Aerobic Granules ◽  
And Function

ABSTRACT Aerobic granules are self-immobilized aggregates of microorganisms and represent a relatively new form of cell immobilization developed for biological wastewater treatment. In this study, both culture-based and culture-independent techniques were used to investigate the bacterial diversity and function in aerobic phenol- degrading granules cultivated in a sequencing batch reactor. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes demonstrated a major shift in the microbial community as the seed sludge developed into granules. Culture isolation and DGGE assays confirmed the dominance of β-Proteobacteria and high-G+C gram-positive bacteria in the phenol-degrading aerobic granules. Of the 10 phenol-degrading bacterial strains isolated from the granules, strains PG-01, PG-02, and PG-08 possessed 16S rRNA gene sequences that matched the partial sequences of dominant bands in the DGGE fingerprint belonging to the aerobic granules. The numerical dominance of strain PG-01 was confirmed by isolation, DGGE, and in situ hybridization with a strain-specific probe, and key physiological traits possessed by PG-01 that allowed it to outcompete and dominate other microorganisms within the granules were then identified. This strain could be regarded as a functionally dominant strain and may have contributed significantly to phenol degradation in the granules. On the other hand, strain PG-08 had low specific growth rate and low phenol degradation ability but showed a high propensity to autoaggregate. By analyzing the roles played by these two isolates within the aerobic granules, a functional model of the microbial community within the aerobic granules was proposed. This model has important implications for rationalizing the engineering of ecological systems.

Start-Up and Cultivation of A2N Denitrifying Phosphorus and Nitrogen Removal System

2012 ◽  
Vol 610-613 ◽  
pp. 1454-1458
Author(s):  
Ming Fen Niu ◽  
Hong Jing Jiao ◽  
Li Xu ◽  
Yan Yu ◽  
Jian Wei
Keyword(s):  
Activated Sludge ◽  
Removal Efficiency ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Nitrogen And Phosphorus ◽  
Start Up ◽  
Two Phases ◽  
Removal System

A2N is two-sludge system, by using the method that first bringing up the cultivation of denitrifying phosphorus removing bacteria (DPB) and nitrification biofilm separately then connecting them, which can start up A2N system successfully. Nitrification biofilm was cultivated in a sequencing batch reactor (SBR). After 30 days, NH4+-N effluent concentration steadily stayed below 0.5mg·L-1.In another SBR, the activated sludge for the enrichment of DPB is from the anaerobic tank, which was firstly operated under anaerobic/aerobic (A/O) condition. After 20 days, PAOs was successfully enriched. Then, the activated sludge was conducted under anaerobic/anoxic/aerobic (A/A/O) condition, maintaining the anaerobic time, gradually increased anoxic time and induced aerobic time. After 30 days DPB was successfully enriched, two phases totally take 50 days. The removal efficiency of total nitrogen and phosphorus are above 85 % and 95 %, so that A2N system was started up successfully.

Advanced nitrogen removal via nitrite from municipal wastewater in a pilot-plant sequencing batch reactor

2009 ◽  
Vol 59 (12) ◽  
pp. 2371-2377 ◽  
Author(s):  
Q. Yang ◽  
X. H. Liu ◽  
Y. Z. Peng ◽  
S. Y. Wang ◽  
H. W. Sun ◽  
...  
Keyword(s):  
Carbon Source ◽  
Process Control ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Pilot Plant ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Nitrite Accumulation ◽  
Advanced Nitrogen Removal

To obtain economically sustainable wastewater treatment, advanced nitrogen removal from municipal wastewater and the feasibility of achieving and stabilizing short-cut nitrification and denitrification were investigated in a pilot-plant sequencing batch reactor (SBR) with a working volume of 54 m3. Advanced nitrogen removal, from summer to winter, with effluent TN lower than 3 mg/L and nitrogen removal efficiency above 98% was successfully achieved in pulsed-feed SBR. Through long-term application of process control in pulsed-feed SBR, nitrite accumulation reached above 95% at normal temperature of 25°C. Even in winter, at the lowest temperature of 13°C, nitrite was still the end production of nitrification and nitrite accumulation was higher than 90%. On the basis of achieving advanced nitrogen removal, short-cut nitrification and denitrification was also successfully achieved. Compare to the pulse-feed SBR with fixed time control, the dosage of carbon source and energy consumption in pulsed-feed SBR with process control were saved about 30% and 15% respectively. In pulsed-feed SBR with process control, nitrogen removal efficiency was greatly improved. Moreover, consumption of power and carbon source was further saved.

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