Population Dynamics of Bacteria for Phosphorus Removal in Sequencing Batch Reactor (SBR) Activated Sludge Processes

1991 ◽  
Vol 23 (4-6) ◽  
pp. 755-763 ◽  
Author(s):  
M. Okada ◽  
A. Murakami ◽  
C. K. Lin ◽  
Y. Ueno ◽  
T. Okubo
Keyword(s):  
Activated Sludge ◽  
Retention Time ◽  
Growth Rates ◽  
Sodium Acetate ◽  
Sequencing Batch Reactor ◽  
Specific Growth ◽  
Batch Reactor ◽  
Organic Substrates ◽  
Specific Growth Rates ◽  
Activated Sludge Processes

Laboratory-scale sequencing batch reactor (SBR) activated sludge processes were operated using synthetic wastewater to clarify the effects of sludge retention time(SRT) and organic substrates on the accumulation of bio-P-bacteria. The accumulation of bio-P-bacteria could be enhanced by wide variation in concentration of organic substrates by giving a short fill period and sufficient anaerobic conditions. However, the accumulation could not be enhanced in the reactor operated with SRT less than 25 d in spite of the higher, more than 0.1 d−1, specific growth rates observed in the isolated strains of bio-P-bacteria. The specific growth rates of bio-P-bacteria were estimated at 0.040 d−1, 0.030 d−1 and 0.035 d−1 in the SBR activated sludge processes fed with sodium acetate (A), glucose and polypeptone (GP) and polypeptone (P), respectively. Therefore, a large sludge retention time would be necessary for the accumulation of bio-P-bacteria. Volatile fatty acids (VFA), such as sodium acetate (A), seemed to be more effective than other organic substrates (GP and P) for the accumulation of bio-P-bacteria in activated sludge ecosystems.

scholarly journals Investigation of the Biological Treatability of Pistachio Processing Industry Wastewaters in a Batch-operated Aerobic Bioreactor

2021 ◽  
Author(s):  
Sevtap Tırınk ◽  
Alper Nuhoğlu ◽  
Sinan Kul
Keyword(s):  
Kinetic Parameters ◽  
Growth Rates ◽  
Specific Growth ◽  
Batch Reactor ◽  
The Other ◽  
Batch Experiments ◽  
Processing Industry ◽  
Specific Growth Rates ◽  
Biological Treatability ◽  
Best Fit

Abstract This study encompasses investigation of treatment of pistachio processing industry wastewaters in a batch reactor under aerobic conditions, calculation of kinetic parameters and comparison of different inhibition models. The mixed microorganism culture used in the study was adapted to pistachio processing industry wastewaters for nearly one month and then concentrations from 50-1000 mg L− 1 of pistachio processing industry wastewaters were added to the medium and treatment was investigated in batch experiments. The Andrews, Han-Levenspiel, Luong and Aiba biokinetic equations were chosen for the correlations between the concentration of pistachio processing industry wastewaters and specific growth rates, and the kinetic parameters in these biokinetic equations were calculated. The µmax, Ks and Ki parameters, included in the Aiba biokinetic equation providing best fit among the other equations, had values calculated as 0.25 h− 1, 19 mg L− 1, and 516 mg L− 1, respectively.

Decrease in Toxicity of Pentachlorophenol and Toluene to Growth of Selected Microbial Consortia and Activated Sludge by Pretreatment with Phanerochaete chrysosporium

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2125-2128 ◽  
Author(s):  
J. Bower Carberry ◽  
L. E. Kovach
Keyword(s):  
Activated Sludge ◽  
Phanerochaete Chrysosporium ◽  
Growth Rates ◽  
Specific Growth ◽  
White Rot ◽  
Microbial Consortia ◽  
Fungal Culture ◽  
Model Compounds ◽  
Fungal Pretreatment ◽  
Specific Growth Rates

The white rot fungus Phanerochaete chrysosporium can degrade toxic compounds under specific nutrient conditions. This attribute was utilized in order to determine the effect of fungal pretreatment on model compounds pentachlorophenol and toluene. The fungal culture was purchased from ATCC, cultured on dextrose agar, and the mycelia harvested to degrade the model compounds. P. chrysosporium was able to degrade up to 74% of initial pentachlorophenol concentration in eight days and up to 31% initial toluene in 31 hours. Specific growth rates of activated sludge and selected microbial consortia were determined on untreated and fungal-pretreated parent model compounds. Specific growth rates for activated sludge and selected microbial consortia were enhanced by fungal pretreatment of both model compounds. Specific growth rates indicated more efficient use of toluene by activated sludge than by toluene-selected microbial consortia, while pentachlorophenol- selected microbial consortia exhibited more favorable growth rates on pentachlorophenol than did activated sludge. Pretreatment results indicated that the toxicity of model compounds was reduced by fungal pretreatment. The growth rates were compared and used as an indication of toxicity reduction which can be exploited at contaminated waste sites or at industrial pretreatment facilities.

scholarly journals Modeling the pH effects on nitrogen removal in the anammox-enriched granular sludge

2016 ◽  
Vol 75 (2) ◽  
pp. 378-386 ◽  
Author(s):  
Xi Lu ◽  
Zhixuan Yin ◽  
Dominika Sobotka ◽  
Kamil Wisniewski ◽  
Krzysztof Czerwionka ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Growth Rates ◽  
Specific Growth ◽  
Batch Reactor ◽  
Mechanistic Model ◽  
Granular Sludge ◽  
Anammox Bacteria ◽  
Ph Effects ◽  
Ammonia Oxidizing Bacteria ◽  
Specific Growth Rates

The aim of the study was to determine the pH effects on nitrogen removal in the anammox-enriched granular sludge. The experimental data were extracted from a 4 L completely-mixed batch reactor with the granular sludge at different initial pH values (6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5) and constant temperature T = 30 °C. Simulations were run in GPS-X 6.4 using a comprehensive mechanistic model Mantis2. Two kinetic parameters, the maximum specific growth rates of ammonia oxidizing bacteria (AOB) and anammox bacteria, were optimized at different pH scenarios. The inhibitory effects of the pH extremes on the anammox-enriched sludge were discussed in terms of the inhibition of free nitrous acid and free ammonia and metabolic mechanisms. Two different pH functions were used to examine the pH effects on the nitrogen removal kinetics. The pH optima for AOB and anammox bacteria were 7.4 and 7.6, respectively. The maximum specific growth rates of AOB and anammox bacteria at the pH optima were 0.81–0.85 d−1 and 0.36–0.38 d−1 (at T = 30 °C). The measured specific anammox activities (SAAs), predicted SAAs by Mantis2 and fitted SAAs by the Michaelis pH function at the pH optima were 0.895, 0.858 and 0.831 gN/(gVSS·d), respectively (VSS: volatile suspended solids).

scholarly journals Ecology of the Microbial Community Removing Phosphate from Wastewater under Continuously Aerobic Conditions in a Sequencing Batch Reactor

2007 ◽  
Vol 73 (7) ◽  
pp. 2257-2270 ◽  
Author(s):  
Johwan Ahn ◽  
Sarah Schroeder ◽  
Michael Beer ◽  
Simon McIlroy ◽  
Ronald C. Bayly ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Sequencing Batch Reactor ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Oxygen Demand ◽  
Extended Period ◽  
Histochemical Staining ◽  
Phosphate Removal ◽  
Organic Substrates ◽  
Aerobic Process

ABSTRACT All activated sludge systems for removing phosphate microbiologically are configured so the biomass is cycled continuously through alternating anaerobic and aerobic zones. This paper describes a novel aerobic process capable of decreasing the amount of phosphate from 10 to 12 mg P liter−1 to less than 0.1 mg P liter−1 (when expressed as phosphorus) over an extended period from two wastewaters with low chemical oxygen demand. One wastewater was synthetic, and the other was a clarified effluent from a conventional activated sludge system. Unlike anaerobic/aerobic enhanced biological phosphate removal (EBPR) processes where the organic substrates and the phosphate are supplied simultaneously to the biomass under anaerobic conditions, in this aerobic process, the addition of acetate, which begins the feed stage, is temporally separated from the addition of phosphate, which begins the famine stage. Conditions for establishing this process in a sequencing batch reactor are detailed, together with a description of the changes in poly-β-hydroxyalkanoate (PHA) and poly(P) levels in the biomass occurring under the feed and famine regimes, which closely resemble those reported in anaerobic/aerobic EBPR processes. Profiles obtained with denaturing gradient gel electrophoresis were very similar for communities fed both wastewaters, and once established, these communities remained stable over prolonged periods of time. 16S rRNA-based clone libraries generated from the two communities were also very similar. Fluorescence in situ hybridization (FISH)/microautoradiography and histochemical staining revealed that “Candidatus Accumulibacter phosphatis” bacteria were the dominant poly(P)-accumulating organisms (PAO) in both communities, with the phenotype expected for PAO. FISH also identified large numbers of betaproteobacterial Dechloromonas and alphaproteobacterial tetrad-forming organisms related to Defluviicoccus in both communities, but while these organisms assimilated acetate and contained intracellular PHA during the feed stages, they never accumulated poly(P) during the cycles, consistent with the phenotype of glycogen-accumulating organisms.

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