Kinetic aspects of biofilm formation on surfaces exposed to drinking water

1995 ◽  
Vol 32 (8) ◽  
pp. 61-65 ◽  
Author(s):  
D. van der Kooij ◽  
H. S. Vrouwenvelder ◽  
H. R. Veenendaal
Keyword(s):  
Drinking Water ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Formation Rate ◽  
Water Distribution Systems ◽  
Acetate Concentration ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution ◽  
The Relationship

Biofilm formation in drinking water distribution systems should be limited to prevent the multiplication of undesirable bacteria and other organisms. Certain types of drinking water with an AOC concentration below 10 μg of acetate-C eq/l can support the growth of Aeromonas. Therefore, the effect of acetate at a concentration of 10 μg of C/l on the biofilm formation rate (BFR) of drinking water with a low AOC concentration (3.2 μg C/l) was determined. Drinking water without acetate had a BFR of 3.9 pg ATP/cm2.day, whereas a BFR value of 362 pg ATP/cm2.day was found with acetate added. These data indicate that a low acetate concentration strongly affects biofilm formation, and that only a small fraction of AOC is available for biofilm formation. Aeromonads did not multiply in the biofilm despite their ability to grow at a concentration of 10 μg of acetate-C/l. Further investigations are needed to elucidate the relationship between substrate concentration and biofilm formation in drinking water distribution systems and the growth of undesirable bacteria in these biofilms.

scholarly journals Biofilm formation potential and chlorine resistance of typical bacteria isolated from drinking water distribution systems

RSC Advances ◽  
2020 ◽  
Vol 10 (52) ◽  
pp. 31295-31304 ◽  
Author(s):  
Zebing Zhu ◽  
Lili Shan ◽  
Fengping Hu ◽  
Zehua Li ◽  
Dan Zhong ◽  
...  
Keyword(s):  
Drinking Water ◽  
Microbial Communities ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Formation Potential ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution ◽  
Safety Of Drinking Water

Biofilms are the main carrier of microbial communities throughout drinking water distribution systems (DWDSs), and strongly affect the safety of drinking water.

scholarly journals Linking flow velocity-regulated EPS production with early-stage biofilm formation in drinking water distribution systems

2020 ◽  
Vol 20 (4) ◽  
pp. 1253-1263
Author(s):  
Yanyan Liu ◽  
Rongrong Shan ◽  
Guowei Chen ◽  
Li Liu
Keyword(s):  
Drinking Water ◽  
Flow Velocity ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Extracellular Polymeric Substances ◽  
Early Stage ◽  
Water Distribution Systems ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

Abstract Hydrodynamics impacts interactions between microbes and their micro-habitats in aqueous systems, thus the study of hydrodynamics is key to understanding the formation and dynamics of biofilms. Yet mechanisms of how microbial responses to hydrodynamics regulate biofilm formation in drinking water distribution systems (DWDS) are underappreciated. Here, we investigated the linkage between early-stage biofilm formation and flow velocity fluctuations in a model DWDS. Results showed that an intermediate velocity (1.0 m/s) enhanced biofilm formation, with the highest biofilm/total cells ratio of 96.91% ± 2.26%. Moreover, the intermediate velocity promoted extracellular polymeric substances (EPS) release, accompanied with lowered zeta potential and elevated hydrophobicity of suspended cells, which could be responsible for surface aggregation. Shifts in biofilm community were observed along with hydrodynamics fluctuations. Intermediate velocity (1.0 m/s) stimulated the dominance of Proteobacteria (78.16%) along with the genus predominance of Pseudomonas, known to secrete large amounts of EPS favoring biofilm formation. Overall, this study provides new understanding of biofilm formation responding to hydrodynamic fluctuations in DWDS.

scholarly journals Influence of phosphate dosing on biofilm development on Drinking Water Distribution Systems infrastructure surfaces

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Gonzalo Del Olmo ◽  
Esther Rosales ◽  
Esther Karunakaran ◽  
Henriette Jensen ◽  
Carolina Calero ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Drinking Water Distribution Systems ◽  
Physico Chemical ◽  
Drinking Water Distribution ◽  
Biofilm Development

Phosphate is added to drinking water by UK water companies as a treatment to prevent the corrosion and metal leaching, like lead, in pipes. However, phosphate is a nutrient for microorganisms, and it can favour biofilm formation in Drinking Water Distribution Systems (DWDS), which can alter the water quality and safety. This study analyses the effect of phosphate addition on biofilm formation over different materials and its consequences for drinking water quality by i) using controlled experimental pipeline facility representative of a real-scale DWDS with high-density polyethylene coupons and ii) using a small-scale DWDS biofilm reactors with lead coupons. Biofilms developed over one month were exposed to the effect of different phosphate dosing and compare with UK normal water phosphate concentrations. During the experiment, physico-chemical analysis of water and microbial analysis of biofilms was carried out. Sequencing analysis of the 16s rRNA gene, from extracted DNA obtained from biofilms, provided information on any bacterial changes, and Scanning Electron Microscopy gave information about the biofilm organization. The results indicate that microorganisms find more difficult to establish and develop biofilms under high phosphate dosing, resulting in biofilms with less cells. Also, some physico-chemical parameter seems to be affected by phosphate dosing, like chlorine and lead. It is expected that differences in the biofilm community will be found depending on phosphate dosing. This study will provide information on the effect of phosphate on biofilm development in different pipes materials, which will facilitate to adjust an optimal phosphate dose to prevent plumbosolvency in DWDS.

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