Comparison of nutrient-removing microbial communities in activated sludge from full-scale MBRs and conventional plants

2013 ◽  
Vol 68 (2) ◽  
pp. 366-371 ◽  
Author(s):  
A. M. Saunders ◽  
P. Larsen ◽  
P. H. Nielsen
Keyword(s):  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Membrane Bioreactors ◽  
Full Scale ◽  
Biological Phosphorus Removal ◽  
Conventional Activated Sludge ◽  
16S Rrna Pyrosequencing ◽  
Biological Phosphorus

The composition of nutrient-removing microbial communities in five full-scale membrane bioreactors (MBRs) was investigated using fluorescence in situ hybridization and 16S rRNA pyrosequencing and compared to similar analyses of conventional activated sludge (CAS) communities. The communities were highly similar but some genera that are always present in enhanced biological phosphorus removal (EBPR) (core groups) were absent in the MBRs. The overall phylogenetic similarity of the communities indicated that these differences were primarily closely related groups. More research is needed to establish the operational significance of the observed differences between MBR and CAS sludge.

Morphology, In-Situ characterization with rRNA targetted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge

1998 ◽  
Vol 38 (8-9) ◽  
pp. 69-76 ◽  
Author(s):  
I. M. Sudiana ◽  
T. Mino ◽  
H. Satoh ◽  
T. Matsuo
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
In Situ Characterization ◽  
Respiratory Quinone ◽  
Biological Phosphorus

The microbial communities in activated sludge acclimated with either acetate or glucose as the major carbon source under phosphorus limited or rich conditions were investigated morphologically, phylogenetically and chemotaxonomically. The sludge with a minimized polyphosphate content was dominated by tetrad shaped bacteria, which were suspected to be ‘glycogen accumulating bacteria (GAOs) or G bacteria’ The sludge containing high polyphosphate was dominated by cluster forming coccus bacteria. Quinone analyses suggested that all the sludge tested contained various ubiquinones and menaquinones, of which the ubiquinones Q-8 and Q-10 were dominant. Analyses with rRNA targeted probes showed that beta sub class of Proteobacteria was most predominant in all sludges tested. Morphological, phylogenetic and chemotaxonomic investigation all indicated that both high and low P sludges are microbiologically diverse.

Upgrading Wastewater Treatment Plants with Anaerobic Selectors

1990 ◽  
Vol 22 (7-8) ◽  
pp. 35-43
Author(s):  
K. D. Tracy ◽  
S. N. Hong
Keyword(s):  
Phosphorus Removal ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
High Rate ◽  
Nitrogen Control ◽  
Biological Phosphorus Removal ◽  
Conventional Activated Sludge ◽  
And Performance ◽  
Activated Sludge Processes ◽  
Biological Phosphorus

The anaerobic selector of the A/0™ process offers many advantages over conventional activated sludge processes with respect to process performance and operational stability. This high-rate, single-sludge process has been successfully demonstrated in full-scale operations for biological phosphorus removal and total nitrogen control in addition to BOD and TSS removal. This process can be easily utilized in upgrading existing treatment plants to meet stringent discharge limitations and to provide capacity expansion. Upgrades of two full-scale installations are described and performance data from the two facilities are presented.

Full-Scale Cyclic Activated Sludge System Phosphorus Removal

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2253-2256 ◽  
Author(s):  
M. C. Goronszy
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Full Scale ◽  
Biological Phosphorus Removal ◽  
Fill Ratio ◽  
Variable Volume ◽  
Activated Sludge System ◽  
Sludge Bulking ◽  
Volume Mode ◽  
Biological Phosphorus

The performance of three full-scale cyclic activated sludge facilities, operated for biological phosphorus removal is demonstrated. The facilities are operated without formal mixed anoxic or anaerobic sequences in a variable volume mode. The system is independent of fill-ratio operation for filamentous sludge bulking control.

Background to Biological Phosphorus Removal

1983 ◽  
Vol 15 (3-4) ◽  
pp. 1-13 ◽  
Author(s):  
James L Barnard
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Plug Flow ◽  
Full Scale ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Paper Briefly ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Biological Phosphorus

This paper briefly summarizes the early work on phosphorus removal in activated sludge plants in the U.S.A. and observed that such removals only occurred in low SRT plants of the plug flow type and in the Phostrip plants, neither designed for full nitrification. The discovery of simultaneous nitrogen and phosphorus removal, as well as full-scale experiments are discussed. The Phoredox process was proposed utilizing internal recycling for the removal of nitrates and an anaerobic first stage in which the incoming feed is used to obtain the necessary anaerobic conditions, essential as a conditioning step for the uptake of phosphorus. Proposed mechanisms are discussed.

Applicability of experience from laboratory reactors with biological phosphorus removal in full-scale plants

2006 ◽  
Vol 54 (1) ◽  
pp. 267-275 ◽  
Author(s):  
E. Tykesson ◽  
L.L. Blackall ◽  
Y. Kong ◽  
P.H. Nielsen ◽  
J. la Cour Jansen
Keyword(s):  
Phosphorus Removal ◽  
Wastewater Treatment Plants ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Fluorescence In Situ Hybridisation ◽  
Biological Phosphorus Removal ◽  
Batch Tests ◽  
P Release ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) has been used at many wastewater treatment plants all over the world for many years. In this study a full-scale sludge with good EBPR was tested with P-release batch tests and combined FISH/MAR (fluorescence in situ hybridisation and microautoradiography). Proposed models of PAOs and GAOs (polyphosphate- and glycogen-accumulating organisms) and microbial methods suggested from studies of laboratory reactors were found to be applicable also on sludge from full-scale plants. Dependency of pH and the uptake of both acetate and propionate were studied and used for calculations for verifying the models and results from microbial methods. All rates found from the batch tests with acetate were higher than in the batch tests with propionate, which was explained by the finding that only those parts of the bacterial community that were able to take up acetate anaerobically were able to take up propionate anaerobically.

Towards exposure of elusive metabolic mixed-culture processes: the application of metaproteomic analyses to activated sludge

2006 ◽  
Vol 54 (1) ◽  
pp. 217-226 ◽  
Author(s):  
P. Wilmes ◽  
P.L. Bond
Keyword(s):  
Protein Expression ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Batch Reactor ◽  
Biological Phosphorus Removal ◽  
Microbial Community Structures ◽  
Proteomic Investigation ◽  
Two Phases ◽  
Biological Phosphorus

Protein expression is a direct reflection of specific microbial activities in any ecosystem. In order to assess protein expression in mixed microbial communities, the feasibility of applying proteomic techniques to activated sludge samples has recently been demonstrated. We report the application of metaproteomics to two activated sludges from a laboratory-scale sequencing batch reactor with dissimilar phosphorus removal performances. Fluorescence in situ hybridization (FISH) revealed that the sludge with good enhanced biological phosphorus removal performance (EBPR) was dominated by Betaproteobacteria (65% of EUBMIX binding cells) and gave positive signals for the Rhodocyclus-type PAO specific probe (59%). The non-EBPR sludge was dominated by tetrad-forming Alphaproteobacteria (75%). With regard to the proteomic investigation, 630 individual protein spots were matched across the replicate groups of the anaerobic and aerobic phases of the EBPR sludge with 9.4% of all spots being statistically different between the two phases. The non-EBPR metaproteomic maps exhibited 590 matched spots with 14.7% statistical differences between the two phases. Overall, the non-EBPR sludge expressed around 30% more significant differences than the EBPR sludge. The comparison of protein expression in the two sludges showed that their metaproteomes were substantially different and this was reflected in their microbial community structures and metabolic transformations.

Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
J.L. Zilles ◽  
C.-H. Hung ◽  
D.R. Noguera
Keyword(s):  
Wastewater Treatment ◽  
Phosphorus Removal ◽  
Fluorescent In Situ Hybridization ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

The objective of this research was to assess the relevance of organisms related to Rhodocyclus in enhanced biological phosphorus removal in full-scale wastewater treatment plants. The presence of these organisms in full-scale plants was first confirmed by fluorescent in situ hybridization. To address which organisms were involved in phosphorus removal, a method was developed which selected polyphosphate-accumulating organisms from activated sludge samples by DAPI staining and flow cytometry. Sorted samples were characterized using fluorescent in situ hybridization. The results of these analyses confirmed the presence of organisms related to Rhodocyclus in full-scale wastewater treatment plants and supported the involvement of these organisms in enhanced biological phosphorus removal. However, a significant fraction of the polyphosphate-accumulating organisms were not related to Rhodocyclus.

The effect of GAOs (glycogen accumulating organisms) on anaerobic carbon requirements in full-scale Australian EBPR (enhanced biological phosphorus removal) plants

2003 ◽  
Vol 47 (11) ◽  
pp. 37-43 ◽  
Author(s):  
A.M. Saunders ◽  
A. Oehmen ◽  
L.L. Blackall ◽  
Z. Yuan ◽  
J. Keller
Keyword(s):  
Phosphorus Removal ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Fluorescence In Situ Hybridisation ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Accumulibacter Phosphatis ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

Glycogen-accumulating organisms (GAOs) were present in six full-scale plants investigated and in all but one made a significant contribution to the amount of volatile fatty acid (VFA) taken up anaerobically. While most plants surveyed contain GAOs, it was demonstrated that it is possible for a full-scale plant to operate with an insignificant GAO population.“Candidatus Accumulibacter phosphatis”were the significant polyphosphate-accumulating organisms (PAOs) in all plants surveyed. “Candidatus Competibacter phosphatis” were found in all plants along with other possible GAOs that were observed but not identified. A significant GAO population will increase the carbon requirements by removing VFA that could otherwise have been used by PAOs. Process optimization minimizing GAOs in full-scale plants would lead to a more efficient use of VFA. Enhanced biological phosphorus removal (EBPR), fluorescence in situ hybridisation (FISH), glycogen accumulating organism (GAO); polyphosphate accumulating organism (PAO);

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