Identification and characterization of anaerobic mercury-resistant bacteria isolated from mercury-polluted sediment

2000 ◽  
Vol 42 (3-4) ◽  
pp. 109-114 ◽  
Author(s):  
M. Narita ◽  
C.C. Huang ◽  
T. Koizumi ◽  
T. Yamagata ◽  
G. Endo
Keyword(s):  
Nucleotide Sequence ◽  
Inorganic Mercury ◽  
Mercury Resistance ◽  
Nucleotide Sequence Analysis ◽  
Resistant Bacteria ◽  
Chromosomal Dna ◽  
Genetic Characteristics ◽  
Polluted Sediment ◽  
Minamata Bay ◽  
Mercury Resistant Bacteria

To develop biotechnology for biological treatment of mercury-contaminated wastes or for bioremediation of mercury-polluted sites, mercury-resistant microorganisms have been isolated and characterized. However, understanding of the mercury resistance mechanism by anaerobic bacteria is lacking. In this study, we tried to isolate anaerobic mercury-resistant bacteria from mercury-polluted sediment in Minamata Bay, Japan. One strain of the bacterial isolates, designated Mersaru, was used for the identification and for the growth capability of mercurials. We also analyzed genetic characteristics of mercury resistance genes (merA and merB gene) from the strain Mersaru. The strain Mersaru, which was isolated from Minamata Bay sediment, was identified as Clostridium butyricum and showed resistance to both inorganic mercury and organomercurials. Furthermore, nucleotide sequence analysis showed that merA and merB genes of the strain Mersaru were identical to those of the B. cereus RC607 an aerobic mercury-resistant bacterium in the nucleotide sequence level. From the pulsed field gel electrophoresis analysis, it is suggest that the mer operon of the strain Mersaru is located on chromosomal DNA.

Mercury Reduction by Bacteria Isolated from Informal Mining Zones

2009 ◽  
Vol 71-73 ◽  
pp. 637-640 ◽  
Author(s):  
J. Sánchez Dávila ◽  
J. Hurtado Custodio
Keyword(s):  
Mercury Resistance ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Specific Primers ◽  
Pure Cultures ◽  
Mercury Reduction ◽  
Mera Gene ◽  
Mercury Resistant Bacteria ◽  
Bioremediation Processes ◽  
Selection Of

The aim of this research was the selection of bacterial strains resistant to mercury, as well as to demonstrate their capacity to reduce mercury in solution when they are inoculated in pure and mixed cultures. Samples of soil from informal mining gold sites in Peru were collected and fifteen mercury resistant bacteria were isolated. Strains RM6, RM7, RM9, RM11, RM12 and RM13 were selected for their capacity to reduce mercury in solution. The six bacterial strains belong to the genus Pseudomonas. Inoculated in pure cultures, these strains reduce mercury in solution although in different percentages: RM9, RM11 an RM12 reduce 93% to 97% of the mercury, while strains RM6, RM7 y RM13 reduce 80% to 85%. The consortium of all six bacterial strains showed a mercury reduction of 84%. Approximately 91% of mercury in solution was reduced in 1 hour and this reaction was not associated to bacterial growth. Using specific primers, the merA gene was amplified from genomic DNA of the bacterial strains, which would suggest the activity of the mer operon as a mechanism of mercury resistance. Due to their ability to reduce mercury in solution, it is advisable to carry out more research on the selected strains since they could be useful in future bioremediation processes.

scholarly journals Mercury-resistant bacteria in the sediment of Minamata Bay.

1988 ◽  
Vol 54 (8) ◽  
pp. 1359-1363 ◽  
Author(s):  
Kunihiko Nakamura ◽  
Tadashi Fujisaki ◽  
Yoshisada Shibata
Keyword(s):  
Resistant Bacteria ◽  
Minamata Bay ◽  
Mercury Resistant Bacteria

scholarly journals Pengaruh pH dan Suhu terhadap Aktivitas Pereduksi Merkuri Bakteri Resisten Merkuri Tinggi Bacillus cereus yang Diisolasi dari Urin Pasien dengan Amalgam Gigi

e-GIGI ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Billy J. Kepel ◽  
Widdhi Bodhi ◽  
. Fatimawali
Keyword(s):  
Bacillus Cereus ◽  
Incubation Temperature ◽  
Mercury Resistance ◽  
Resistant Bacteria ◽  
Growth Media ◽  
Mercury Compounds ◽  
Reducing Activity ◽  
Mercury Detoxification ◽  
Incubation Temperatures ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a very toxic compound to humans, therefore, a method to overcome its presence in the environment is required. Detoxification of mercury can be done by using mercury resistant bacteria. Mercury-resistant bacteria Bacillus cereus isolate FUA have been obtained from the urine of patients with dental mercury amalgam. This study was aimed to determine the mercury detoxification activity of Bacillus cereus isolate FUA at varying pH medium and incubation temperature. The study was carried out by growing Bacillus cereus isolate FUA on oblique media, then were planted in the growth media of LB broth containing mercury compounds of 10 ppm HgCl2 with varying pHs of 5, 7, and 9 and incubation temperatures of 15, 25 and 35oC. The amount of bacterial growth was analyzed by using spectrophotometer and mercury levels were analyzed by using CV-AAS method. The results showed that the growth and mercury reducing activity of Bacillus cereus isolate FUA were optimum at pH 7 and incubation temperature of 35oC. In conclusion, the growth of Bacillus cereus isolate FUA and its mercury reducing activity were optimum at pH 7 and temperature of 35oC. It is expected that the results of this study can be the basis for further research on the process of mercury detoxificationKeywords: Bacillus cereus, urine, mercury resistance, pH, temperature Abstrak: Merkuri adalah senyawa yang sangat beracun bagi manusia sehingga diperlukan metode untuk mengatasi keberadaannya di lingkungan. Detoksifikasi merkuri dapat dilakukan dengan menggunakan bakteri resisten merkuri. Bakteri yang resisten merkuri Bacillus cereus isolat FUA telah diperoleh dari urin pasien dengan amalgam gigi. Penelitian ini bertujuan untuk mengetahui aktivitas detoksifikasi merkuri Bacillus cereus isolat FUA pada berbagai variasi pH medium dan suhu inkubasi. Bakteri Bacillus cereus isolat FUA ditumbuhkan pada media miring, kemudian ditanam pada media pertumbuhan bakteri LB broth yang mengandung senyawa merkuri 10 ppm HgCl2 dengan berbagai pH 5, 7 dan 9 dan suhu inkubasi 15, 25 dan 35oC. Jumlah pertumbuhan bakteri dianalisis menggunakan spektrofotometer dan kadar merkuri dianalisis menggunakan metode CV-AAS. Hasil penelitian menunjukkan bahwa aktivitas pertumbuhan dan aktivitas pereduksi merkuri Bacillus cereus isolat FUA optimum pada lingkungan pertumbuhan dengan pH 7 dan suhu inkubasi 35oC. Simpulan penelitian ini ialah aktivitas pertumbuhan dan pereduksi merkuri Bacillus cereus isolat FUA yang optimum pada pH 7 dan suhu 35oC. Diharapkan hasil penelitian ini dapat menjadi dasar untuk penelitian lebih lanjut tentang proses detoksifikasi merkuri.Kata kunci: Bacillus cereus, urin, resistensi merkuri, pH, suhu

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI

2011 ◽  
Vol 15 (1) ◽  
pp. 282 ◽  
Author(s):  
Fatimawali Fatimawali ◽  
Fatmawaty Badaruddin ◽  
Irawan Yusuf
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pure Culture ◽  
Culture Media ◽  
Inorganic Mercury ◽  
River Estuary ◽  
Nutrient Broth ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mercury Resistant Bacteria

ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI Fatimawali1), Fatmawaty Badaruddin2), Irawan Yusuf 2) 1)Dosen Fakultas  Kedokteran Unsrat Manado dan Mahasiswa Program Doktor Fakultas Kedokteran Universitas Hasanuddin Makassar; 2)Dosen Fakultas Kedokteran Universitas Hasanuddin Makassar ABSTRAK Merkuri adalah logam berat yang bersifat sangat toksik, mempunyai afinitas terhadap gugus thiol protein.  Keberadaan merkuri  dilingkungan, karena secara alamiah maupun karena aktifitas antrofogenik. Di Kabupaten Bolaang Mongondow terdapat beberapa penambang emas rakyat yang menggunakan merkuri untuk mengekstrak emas dari batuan atau ore dan membuang limbah merkuri secara bebas ke lingkungan dan terbawa air hujan sampai kepada perairan.  Sedimen perairan yang terkontaminasi merkuri dapat ditumbuhi bakteri resisten merkuri.  Bakteri resisten merkuri anorhanik dapat digunakan untuk bioremediasi perairan, karena bakteri tersebut mampu mereduksi merkuri anorganik menjadi logam merkuri yang mudah menguap dan kurangtoksik.  Dan merupakan sumber pencemaran merkuri.  Bakteri resisten merkuri mempunyai operon mer yang biasanya terkandung dalam plasmid.  Penelitian ini bertujuan untuk mengisolasi bakteri resisten merkuri anorganik, dan menganalisis kemampuannya dalam mereduksi HgCl2 dalam media nutrient broth.  Bakteri resisten merkuri tinggi, dilakukan analisis Gen 16S rRNAnya untuk mengetahuis pesiesnya.  Hasil penelitian menunjukkan bahwa isolat A1.1.1 merupakan bakteri spesies Klebsiella pneumoniae, mampu mereduksi HgCl2 75% dalam waktu 1 jam, 92% dalam waktu 12 jam dan 99,4%  dalam waktu 24 jam Kata Kunci: bakteri, detoksifikasi,  HgCl2,  Klebsiella pneumoniae   ISOLATION  AND  IDENTIFICATION OF MERCURY-RESISTANT BACTERIUM FROM SARIO RIVER ESTUARY THAT CAN BE USED TO DETOXIFY INORGANIK MERCURY WASTES ABSTRACT Mercury (Hg) is well known for its high toxicity and strong affinity toward the thiol group of proteins. When Hg released into the environment in substantial quantities through natural events and anthropogenic activities . In District of Bolaang Mongondow, many miners who are a source of mercury pollution of aquatic environments to which they use to extract gold from the rock or ore. Waters sediment contaminated with mercury, can be overgrown with microorganism such as bacteria that are resistant to mercury.  Mercury-resistant bacteria can be used for bioremediation of waters due to the ability of these bacteria to reduce toxic inorganic mercury into mercury metal which is volatile and less toxic. Mercury-resistant bacteria has mer Operon which usually contained in the plasmid.  The aim of this study was the selection of bacterial strains resistant to inorganic mercury, as well as to show their capacity to reduce mercury  in pure culture media Nutrient Broth. Twelve isolate bacteria was selected for its capacity to reduce mercury HgCl2 in culture media nutrient broth. The one bacterial strains belong to the spesies Klebsiella pneumoniae. Inoculated in pure culture, these strain showed a mercury reduction of 75% in 1 hour, 92% in 12 hours, and  99,4% in 24 hours. Keywords : bacteria , detoxification, HgCl2 ,  Klebsiella pneumoniae

Nucleotide sequence analysis of a mouse Y chromosomal DNA fragment containing Bkm and LINE elements

Genetica ◽  
1992 ◽  
Vol 87 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Y. Nishioka ◽  
B. M. Dolan ◽  
A. Fiorellino ◽  
V. F. Prado
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Chromosomal Dna ◽  
Dna Fragment ◽  
Line Elements

scholarly journals Genetic Characterisation of Spring Viraemia of Carp Virus Strains in Russia

2021 ◽  
Author(s):  
Aleksey Perchun ◽  
Dmitry Pavlov ◽  
Vladimir Melnikov ◽  
Aleksey Antonychev ◽  
Nikolay Zinyakov ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Genetic Characteristics ◽  
Republic Of Moldova ◽  
The Russian Federation ◽  
The Republic ◽  
High Level ◽  
Virus Strains

Abstract The paper presents genetic characteristics of two strains of the spring viraemia of carp virus strains Kirov/08 and Orenburg/14 isolated in the Kirov and Orenburg Oblasts of the Russian Federation, respectively. The nucleotide sequence analysis of the 516-bp fragment of Kirov/08 and Orenburg/14 G gene showed 9.5% difference. The phylogenetic analysis demonstrated a high level of homology of these strains to SVC viruses, isolated earlier in Russia, Ukraine and the Republic of Moldova.

scholarly journals STUDI POPULASI BAKTERI RESISTEN MERKURI DI DAERAH ALIRAN SUNGAI TONDANO, KELURAHAN KETANG BARU, MANADO

2011 ◽  
Vol 11 (1) ◽  
pp. 26
Author(s):  
Aaltje E. Manampiring ◽  
Billy J. Keppel
Keyword(s):  
Escherichia Coli ◽  
Mercury Resistance ◽  
Resistant Bacteria ◽  
Cross Sectional ◽  
Watershed Area ◽  
Descriptive Research ◽  
Mercury Resistant Bacteria

Kadar merkuri yang tinggi di perairan umumnya dapat mempengaruhi keadaan biota termasuk bakteri yang resisten terhadap merkuri. Penelitian deskriptif yang menggunakan metode cross sectional bertujuan untuk menguji resistensi bakteri di aliran sungai Tondano, Kelurahan Ketang Baru, Manado terhadap merkuri. Escherichia coli dan Bacillus careus (isolat1,1 dan 2,2) hanya dapar tumbuh pada konsentrasi HgCl2 0,02%. Lactobacillus sp. dan Veillonella parvula (isolat 1,2 2,1 3,1 dan 3,2) tumbuh pada konsentrasi HgCl2 0,06%. Lactobacillus sp. (isolat 3,1) saja yang tumbuh pada konsentrasi HgCl2 0,1% dan tidak ada bakteri yang mampu tumbuh pada konsentrasi HgCl2 0,2%. STUDY ON POPULATION OF MERCURY-RESISTANT BACTERIAIN THE WATERSHED AREA OF TONDANO RIVER,KELURAHAN KETANG BARU, MANADOHigh concentration of mercury in the watershed area can affect biota condition, including mercury-resistant bacteria. This descriptive research with cross-sectional method aimed to evaluate the mercury-resistance of bacteria in watershed area of Tondano, Kelurahan Ketang Baru, Manado. Escherichia coli and Bacillus careus (isolates 1,1, dan 2,2) were able to grow in HgCl2 0,02%. Lactobacillus sp. and Veillonella parvula (isolates 1,2 2,1 3,1 and 3,2) grow in HgCl2 0,06%. Lactobacilus sp. (isolate 3,1) only grow in HgCl2 0,1% and none of bacteria could grow in HgCl2 0,2%.

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