scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI

2011 ◽  
Vol 15 (1) ◽  
pp. 282 ◽  
Author(s):  
Fatimawali Fatimawali ◽  
Fatmawaty Badaruddin ◽  
Irawan Yusuf
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pure Culture ◽  
Culture Media ◽  
Inorganic Mercury ◽  
River Estuary ◽  
Nutrient Broth ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mercury Resistant Bacteria

ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI Fatimawali1), Fatmawaty Badaruddin2), Irawan Yusuf 2) 1)Dosen Fakultas  Kedokteran Unsrat Manado dan Mahasiswa Program Doktor Fakultas Kedokteran Universitas Hasanuddin Makassar; 2)Dosen Fakultas Kedokteran Universitas Hasanuddin Makassar ABSTRAK Merkuri adalah logam berat yang bersifat sangat toksik, mempunyai afinitas terhadap gugus thiol protein.  Keberadaan merkuri  dilingkungan, karena secara alamiah maupun karena aktifitas antrofogenik. Di Kabupaten Bolaang Mongondow terdapat beberapa penambang emas rakyat yang menggunakan merkuri untuk mengekstrak emas dari batuan atau ore dan membuang limbah merkuri secara bebas ke lingkungan dan terbawa air hujan sampai kepada perairan.  Sedimen perairan yang terkontaminasi merkuri dapat ditumbuhi bakteri resisten merkuri.  Bakteri resisten merkuri anorhanik dapat digunakan untuk bioremediasi perairan, karena bakteri tersebut mampu mereduksi merkuri anorganik menjadi logam merkuri yang mudah menguap dan kurangtoksik.  Dan merupakan sumber pencemaran merkuri.  Bakteri resisten merkuri mempunyai operon mer yang biasanya terkandung dalam plasmid.  Penelitian ini bertujuan untuk mengisolasi bakteri resisten merkuri anorganik, dan menganalisis kemampuannya dalam mereduksi HgCl2 dalam media nutrient broth.  Bakteri resisten merkuri tinggi, dilakukan analisis Gen 16S rRNAnya untuk mengetahuis pesiesnya.  Hasil penelitian menunjukkan bahwa isolat A1.1.1 merupakan bakteri spesies Klebsiella pneumoniae, mampu mereduksi HgCl2 75% dalam waktu 1 jam, 92% dalam waktu 12 jam dan 99,4%  dalam waktu 24 jam Kata Kunci: bakteri, detoksifikasi,  HgCl2,  Klebsiella pneumoniae   ISOLATION  AND  IDENTIFICATION OF MERCURY-RESISTANT BACTERIUM FROM SARIO RIVER ESTUARY THAT CAN BE USED TO DETOXIFY INORGANIK MERCURY WASTES ABSTRACT Mercury (Hg) is well known for its high toxicity and strong affinity toward the thiol group of proteins. When Hg released into the environment in substantial quantities through natural events and anthropogenic activities . In District of Bolaang Mongondow, many miners who are a source of mercury pollution of aquatic environments to which they use to extract gold from the rock or ore. Waters sediment contaminated with mercury, can be overgrown with microorganism such as bacteria that are resistant to mercury.  Mercury-resistant bacteria can be used for bioremediation of waters due to the ability of these bacteria to reduce toxic inorganic mercury into mercury metal which is volatile and less toxic. Mercury-resistant bacteria has mer Operon which usually contained in the plasmid.  The aim of this study was the selection of bacterial strains resistant to inorganic mercury, as well as to show their capacity to reduce mercury  in pure culture media Nutrient Broth. Twelve isolate bacteria was selected for its capacity to reduce mercury HgCl2 in culture media nutrient broth. The one bacterial strains belong to the spesies Klebsiella pneumoniae. Inoculated in pure culture, these strain showed a mercury reduction of 75% in 1 hour, 92% in 12 hours, and  99,4% in 24 hours. Keywords : bacteria , detoxification, HgCl2 ,  Klebsiella pneumoniae

scholarly journals Microscopy and Microanalysis of an Extreme Case of Salt and Biodegradation in 17th Century Wall Paintings

2015 ◽  
Vol 21 (3) ◽  
pp. 606-616 ◽  
Author(s):  
Milene Gil ◽  
Maria Rosário Martins ◽  
Maria Luisa Carvalho ◽  
Cátia Souto ◽  
Stephane Longelin ◽  
...  
Keyword(s):  
Culture Media ◽  
Extreme Case ◽  
Biological Assessment ◽  
Salt Weathering ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mineralogical Characterization ◽  
Fungal Isolation ◽  
X Ray ◽  
Standard Culture

AbstractThe present study characterizes the main deterioration mechanisms affecting the early 17th frescoes of Casa de Fresco, the only known example in Portugal of a semi-underground leisure room richly decorated with a balcony over a water well. Frescoes from the vault are at risk due to salt weathering and biodeterioration. The aim of the research was identification of the deterioration materials, determination of their origin, and their effect on the frescoes before future intervention. Scanning electron microscopy with an energy-dispersive X-ray detector (SEM-EDS) was used to determine salt morphology and microanalysis. The mineralogical characterization was performed by X-ray powder diffraction, complemented with µ-Raman and µ-Fourier transform infrared spectroscopy. Biological assessment was evaluated with optical microscopy and SEM-EDS. Bacterial and fungal isolation and identification were performed using standard culture media and methods according to Bergey’s Manual of Systematic Bacteriology and from the Compendium of Soil Fungi. The results show that Ca and Ca-Mg carbonates from the paint renderings are the predominant salt species affecting the site. Bacterial strains from the genera Bacillus and Pseudomonas and fungal strains from the Cladosporium spp. and Penicillium spp. were isolated in the salt formations, within and between the mortar layers. Azurite, malachite, and smalt paint layers are the most affected by the weathering conditions.

Mercury Reduction by Bacteria Isolated from Informal Mining Zones

2009 ◽  
Vol 71-73 ◽  
pp. 637-640 ◽  
Author(s):  
J. Sánchez Dávila ◽  
J. Hurtado Custodio
Keyword(s):  
Mercury Resistance ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Specific Primers ◽  
Pure Cultures ◽  
Mercury Reduction ◽  
Mera Gene ◽  
Mercury Resistant Bacteria ◽  
Bioremediation Processes ◽  
Selection Of

The aim of this research was the selection of bacterial strains resistant to mercury, as well as to demonstrate their capacity to reduce mercury in solution when they are inoculated in pure and mixed cultures. Samples of soil from informal mining gold sites in Peru were collected and fifteen mercury resistant bacteria were isolated. Strains RM6, RM7, RM9, RM11, RM12 and RM13 were selected for their capacity to reduce mercury in solution. The six bacterial strains belong to the genus Pseudomonas. Inoculated in pure cultures, these strains reduce mercury in solution although in different percentages: RM9, RM11 an RM12 reduce 93% to 97% of the mercury, while strains RM6, RM7 y RM13 reduce 80% to 85%. The consortium of all six bacterial strains showed a mercury reduction of 84%. Approximately 91% of mercury in solution was reduced in 1 hour and this reaction was not associated to bacterial growth. Using specific primers, the merA gene was amplified from genomic DNA of the bacterial strains, which would suggest the activity of the mer operon as a mechanism of mercury resistance. Due to their ability to reduce mercury in solution, it is advisable to carry out more research on the selected strains since they could be useful in future bioremediation processes.

scholarly journals Isolasi dan Identifikasi Bakteri Resisten Merkuri pada Plak Gigi dan Urin

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Priscilla R. Lumanto ◽  
Aaltje Manampiring ◽  
Fona Budiarso
Keyword(s):  
Dental Plaque ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Microbiology Laboratory ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Metal Compounds ◽  
Pharmacy Department ◽  
Cross Sectional Design ◽  
Mercury Resistant Bacteria

Abstract: Mercury toxic effects could be reduced by the presence of mercury resistant microorganisms, such as mercury resistant bacteria. These bacteria possess mercury resistant gene, the operon mere gene. Resistance could also occur through genes that code mercury resistant characteristics and of other heavy metal compounds that generally located within the plasmid. This study was aimed to obtain the profile of bacteria in dental plaque and urine that were resistant to mercury. This was a descriptive prospective study with a cross-sectional design. Isolation and identification of the bacteria were conducted at Microbiology Laboratory of Pharmacy Department, Sam Ratulangi University. The results obtained bacteria of Bacillus sp. which were sensitive to antibiotics ciprofloxacin, erythromycin, and doxycyclin. Conclusion: Mercury resistent bacteria in dental plaque and urine were sensitive to antibiotics ciprofloxacin, erythromycin, and doxycyclin.Keywords: mercury resistent bacteria, dental plaque, urine Abstrak: Toksisitas merkuri dapat berkurang oleh adanya mikroorganisme resisten merkuri, misalnya bakteri resisten merkuri. Hal ini terjadi karena bakteri memiliki gen yang resisten terhadap merkuri, yaitu gen operon mer. Resistensi juga dapat melalui gen yang mengkode sifat resisten terhadap merkuri dan senyawa logam berat lainnya yang umumnya terletak pada plasmid. Penelitian ini bertujuan untuk mengetahui gambaran bakteri dari plak gigi dan urin yang resistensi terhadap merkuri. Jenis penelitian ialah deskriptif prospektif dengan desain potong lintang. Isolasi dan identifikasi bakteri dari plak gigi dan urin yang resisten terhadap merkuri dilakukan di Laboratorium Mikrobiologi Farmasi FMIPA Unsrat. Hasil penelitian mendapatkan bakteri dari sampel gigi dan urine ialah bakteri Bacillus sp. yang sensitif terhadap antibiotik siprofloksasin, eritromisin, dan doksisiklin. Simpulan: Bakteri resisten terhadap merkuri dalam sampel plak gigi dan urin ialah Bacillus sp. yang sensitif terhadap antibiotik siprofloksasin, eritromisin, dan doksisiklin.Kata kunci: bakteri resisten merkuri, plak gigi, urin

Microbiological status of some street iftar items collected from chalk bazar in Dhaka city, Bangladesh

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 236-240
Author(s):  
I.T. Nur ◽  
M. Talukder ◽  
T.R. Das ◽  
M. Asaduzzaman ◽  
F. Feroz ◽  
...  
Keyword(s):  
Culture Media ◽  
Microbiological Quality ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Street Food ◽  
Food Items ◽  
Viable Bacteria ◽  
Staphylococcus Spp

In Dhaka, the capital of Bangladesh and one of the most densely populated cities of the world, different categories of street foods are widely consumed by all classes of people, especially for iftar during the holy month of Ramadan. The objective of this research was to assess the microbiological quality of street iftar food items collected from a street in Chalk Bazar locality of Dhaka along with the antibiogram profile of the bacterial isolates. A total of 74 samples belong to ten different categories of street food items and 8 different types of street vended juices were collected aseptically. The bacteria were isolated by using different culture media. The antibiotic susceptibility of the bacterial isolates was determined by the disc diffusion method. In case of street food total viable bacteria (on average of 6 log10 CFU/g). On the other hand, an extended number of total viable bacteria were encountered in all juices samples which also on an average of 6 log10 CFU/mL. Fungi, Pseudomonas spp. and Staphylococcus spp. were found in the majority of the samples irrespective of the categories. Few samples were contaminated with Escherichia coli and Klebsiella spp. Most of the cultivated bacterial strains exhibited resistance against commonly used antibiotics, while several isolates were noted to be multi-drug resistant. The present study revealed a huge array of microbial load which indicates a high risk to public health. Presence of antibiotic-resistant bacteria heightened the risk by many folds and urges the need for frequent surveillance.

scholarly journals Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from poultry farms in Blitar, Indonesia

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
DIAN AYU PERMATASARI ◽  
ADIANA MUTAMSARI WITANINGRUM ◽  
FRESHINTA JELLIA WIBISONO ◽  
Mustofa Helmi Effendi
Keyword(s):  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Isolation And Identification ◽  
Layer Chicken ◽  
Confirmation Test ◽  
Poultry Farms ◽  
The Impact

Abstract. Permatasari DA, Witaningrum AM, Wibisono FJ, Effendi MH. 2020. Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strain isolated from poultry farms in Blitar, Indonesia. Biodiversitas 21: 4642-4647. Antibiotics are commonly used as therapy and disease control in humans and animals. However, the widespread use of antibiotics may also trigger the rise of antibiotic resistance. Therefore, the aim of this study was to study the occurrence of Multidrug-Resistant (MDR) of Klebsiella pneumoniae from poultry farms in Blitar. Chicken cloacal swabs were collected from 63 poultry farms (32 broilers and 31 layers) were taken randomly, using total samples 160 broilers and 155 layers. The collected swab samples were inoculated on MacConkey agar medium for isolation and identification. Single colonies were isolated after primary positive cultures and identified by using the IMViC test and TSIA. 28 (8.88%) out of samples were positive for Klebsiella pneumoniae. The antimicrobial confirmation test showed that 53.57% of the K. pneumoniae isolates were Multi-Drug Resistance (MDR) bacteria. The percentage of MDR bacteria against different antibiotics included ampicillin 75%, Erythromycin 42.86%, Tetracycline 35.72%, Sulfamethoxazole 32.14% and Streptomycin 21.4%. This study confirms that the percentage of resistant isolates of Klebsiella pneumoniae from 5 antimicrobial agents of broiler chicken is higher than layer chicken. The presence of multidrug-resistant bacteria is a threat to public health and livestock. The impact of these conditions leaves a limited treatment option as chicken farmers in Indonesia still using antibiotics without veterinarian supervision.

Mechanisms of mercury bioremediation

2002 ◽  
Vol 30 (4) ◽  
pp. 672-674 ◽  
Author(s):  
A. M. M. Essa ◽  
L. E. Macaskie ◽  
N. L. Brown
Keyword(s):  
Heavy Metals ◽  
Klebsiella Pneumoniae ◽  
Capture Efficiency ◽  
Mercury Pollution ◽  
Resistant Bacteria ◽  
Toxic Heavy Metals ◽  
Metallic Mercury ◽  
Mercury Volatilization ◽  
Mercury Detoxification ◽  
Mercury Resistant Bacteria

Mercury is one of the most toxic heavy metals, and has significant industrial and agricultural uses. These uses have led to severe localized mercury pollution. Mercury volatilization after its reduction to the metallic form by mercury-resistant bacteria has been reported as a mechanism for mercury bioremediation [Brunke, Deckwer, Frischmuth, Horn, Lunsdorf, Rhode, Rohricht, Timmis and Weppen (1993) FEMS Microbiol. Rev. 11, 145–152; von Canstein, Timmis, Deckwer and Wagner-Dobler (1999) Appl. Environ. Microbiol. 65, 5279–5284]. The reduction/volatilization system requires to be studied further, in order to eliminate the escape of the metallic mercury into the environment. Recently we have demonstrated three different mechanisms for mercury detoxification in one organism, Klebsiella pneumoniae M426, which may increase the capture efficiency of mercury.

Identification and characterization of anaerobic mercury-resistant bacteria isolated from mercury-polluted sediment

2000 ◽  
Vol 42 (3-4) ◽  
pp. 109-114 ◽  
Author(s):  
M. Narita ◽  
C.C. Huang ◽  
T. Koizumi ◽  
T. Yamagata ◽  
G. Endo
Keyword(s):  
Nucleotide Sequence ◽  
Inorganic Mercury ◽  
Mercury Resistance ◽  
Nucleotide Sequence Analysis ◽  
Resistant Bacteria ◽  
Chromosomal Dna ◽  
Genetic Characteristics ◽  
Polluted Sediment ◽  
Minamata Bay ◽  
Mercury Resistant Bacteria

To develop biotechnology for biological treatment of mercury-contaminated wastes or for bioremediation of mercury-polluted sites, mercury-resistant microorganisms have been isolated and characterized. However, understanding of the mercury resistance mechanism by anaerobic bacteria is lacking. In this study, we tried to isolate anaerobic mercury-resistant bacteria from mercury-polluted sediment in Minamata Bay, Japan. One strain of the bacterial isolates, designated Mersaru, was used for the identification and for the growth capability of mercurials. We also analyzed genetic characteristics of mercury resistance genes (merA and merB gene) from the strain Mersaru. The strain Mersaru, which was isolated from Minamata Bay sediment, was identified as Clostridium butyricum and showed resistance to both inorganic mercury and organomercurials. Furthermore, nucleotide sequence analysis showed that merA and merB genes of the strain Mersaru were identical to those of the B. cereus RC607 an aerobic mercury-resistant bacterium in the nucleotide sequence level. From the pulsed field gel electrophoresis analysis, it is suggest that the mer operon of the strain Mersaru is located on chromosomal DNA.

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