Genetic Characterisation of Spring Viraemia of Carp Virus Strains in Russia

Abstract The paper presents genetic characteristics of two strains of the spring viraemia of carp virus strains Kirov/08 and Orenburg/14 isolated in the Kirov and Orenburg Oblasts of the Russian Federation, respectively. The nucleotide sequence analysis of the 516-bp fragment of Kirov/08 and Orenburg/14 G gene showed 9.5% difference. The phylogenetic analysis demonstrated a high level of homology of these strains to SVC viruses, isolated earlier in Russia, Ukraine and the Republic of Moldova.

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Complete Nucleotide Sequence Analysis of Plasmids in Strains ofStaphylococcus aureusClone USA300 Reveals a High Level of Identity among Isolates with Closely Related Core Genome Sequences

Journal of Clinical Microbiology ◽

10.1128/jcm.01050-10 ◽

2010 ◽

Vol 48 (12) ◽

pp. 4504-4511 ◽

Cited By ~ 43

Author(s):

Adam D. Kennedy ◽

Stephen F. Porcella ◽

Craig Martens ◽

Adeline R. Whitney ◽

Kevin R. Braughton ◽

...

Keyword(s):

Sequence Analysis ◽

Nucleotide Sequence ◽

Complete Nucleotide Sequence ◽

Core Genome ◽

Nucleotide Sequence Analysis ◽

Genome Sequences ◽

High Level

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THE OCCURRENCE AND SEQUENCE ANALYSIS OF MILKY HEMOLYMPH DISEASE IN LOBSTER PANULIRUS HOMARUS FROM DIFFERENT LOCATIONS IN INDONESIA

Journal of Aquaculture and Fish Health ◽

10.20473/jafh.v9i1.15913 ◽

2020 ◽

Vol 9 (1) ◽

pp. 48

Author(s):

Sudewi Sudewi ◽

Zeny Widiastuti ◽

Ida Komang Wardana ◽

Ketut Mahardika

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Nucleotide Sequence ◽

Spiny Lobster ◽

Pcr Analysis ◽

Nucleotide Sequence Analysis ◽

Disease Occurrence ◽

Geographical Regions ◽

Disease Agent ◽

Relationship Of

Among the high significance diseases in spiny lobster farming, Milky Hemolymph Disease of Spiny Lobster (MHD-SL) is considered as the most devastating disease which caused in mortality up to 80 % and morbidity to 100%. Investigations of this disease were mostly performed for farmed lobster and no observation was conducted for wild lobster. This study was carried out first, to investigate milky hemolymph disease occurrence both in wild and farmed spiny lobster Panulirus homarus from different locations by PCR analysis. Second, to perform nucleotide sequence analysis in order to identify, and to describe relationship of the milky disease agent obtained in this study with the disease agent from several geographical regions by phylogenetic analysis. Adult farmed lobsters were collected from Lombok, Pangandaran and Pegametan, while wild lobsters were obtained from Jembrana, Lombok, and Banyuwangi coastal areas. One farmed lobster from Pegametan and two farmed lobsters from Lombok were infected with the milky disease. Nucleotide sequence analysis demonstrated that the causative agent of milky disease obtained in this study (MHD-1, 2, and 3) exhibited 99% homologous nucleotide sequence with milky disease agent from Vietnam that was uncultured Rickettsia-like Bacteria (RLB). As a consequence, further work is needed most importantly on how to cultivate milky disease agent in order to find proper methods to alleviate milky disease problems. This paper is the first report on phylogenetic analysis of milky disease from Indonesia that pointed out a closed relationship between milky disease in the present study and from Vietnam, Madagascar, Mozambique and Tanzania.

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