The use of rotifers for limiting filamentous bacteria Type 021N, a bacteria causing activated sludge bulking

2013 ◽  
Vol 67 (7) ◽  
pp. 1557-1563 ◽  
Author(s):  
Wioleta Kocerba-Soroka ◽  
Edyta Fiałkowska ◽  
Agnieszka Pajdak-Stós ◽  
Beata Klimek ◽  
Ewa Kowalska ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Filamentous Bacteria ◽  
Sludge Bulking ◽  
Microthrix Parvicella ◽  
Lecane Inermis

The excessive growth of filamentous bacteria and the resultant bulking of activated sludge constitute a serious problem in numerous wastewater treatment plants. Lecane inermis rotifers were previously shown to be capable of reducing the abundance of Microthrix parvicella and Nostocoida limicola in activated sludge. In the present study, the effectiveness of four Lecane clones in reducing the abundance of Type 021N filamentous bacteria was investigated. Three independent experiments were carried out on activated sludge from three different treatment plants. We found that Lecane rotifers are efficient consumers of Type 021N filaments.

scholarly journals Deep learning-based morphology classification of activated sludge flocs in wastewater treatment plants

2021 ◽  
Author(s):  
Hisashi Satoh ◽  
Yukari Kashimoto ◽  
Naoki Takahashi ◽  
Takashi Tsujimura
Keyword(s):  
Wastewater Treatment ◽  
Deep Learning ◽  
Activated Sludge ◽  
Morphological Change ◽  
Wastewater Treatment Plants ◽  
Filamentous Bacteria ◽  
Sludge Flocs

A deep learning-based two-label classifier 1 recognized a 20% morphological change in the activated flocs. Classifier-2 quantitatively recognized an abundance of filamentous bacteria in activated flocs.

Performances of three R-AN-D-N wastewater treatment plants in the Czech Republic

2004 ◽  
Vol 50 (7) ◽  
pp. 249-255 ◽  
Author(s):  
Z. Bejvl ◽  
P. Matuška ◽  
J. Stara ◽  
P. Chudoba
Keyword(s):  
Wastewater Treatment ◽  
Czech Republic ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Industrial Effluents ◽  
Filamentous Bacteria ◽  
The Czech Republic ◽  
Treated Sewage ◽  
Nutrient Removal Efficiency

The evolution of Czech standards requires higher efficiency of nutrient removal from municipal wastewaters. At the beginning of the last decade of 20th century, a new activated sludge configuration called R-AN-D-N process has been described, successfully tested and now largely used at several wastewater treatment plants (WWTP) in the Czech republic. The main feature of the R-AN-D-N process is the introduction of a regeneration zone in sludge recycle, which enables to increase sludge age in the system without any substantial increase in WWTP volume. Performances of three Czech large WWTP with R-AN-D-N configuration have been monitored and compared within a period of one and a half years. The results confirmed excellent nutrient removal efficiency for wastewaters with different proportion between sewage and industrial effluents. Two of three monitored WWTP received wastewaters from breweries (Budweiser and Pilsner Urquell). The settleability of activated sludge from all three WWTP was correct, with SVI values usually ranging from 50 to 150 ml/g. Monitoring of sludge composition indicated proliferation of several filamentous bacteria, particularly types 0581, 0092 and M. parvicella. No severe bulking events were observed. Finally, the operational costs expressed in CZK (Czech crown: 1CZK = €0.0322) per cubic metre of treated sewage or per capita amounts respectively from 2.24 to 6.52, and from 285 to 342.

Control of Activated Sludge Bulking: From the Lab to the Plant

1989 ◽  
Vol 21 (6-7) ◽  
pp. 717-726 ◽  
Author(s):  
R. Pujol ◽  
P. Boutin
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Common Type ◽  
Test Method ◽  
Mixing Zone ◽  
Operational Conditions ◽  
Sludge Bulking ◽  
Technical Aid

Activated sludge bulking often occurs in French wastewater treatment plants. Precise identification of filaments and better knowledge of their ecophysiology helped to clarify their re1ations with the operational conditions of plants. Effective techniques were therefore developed for control. The “mixing zone” is suitable for many cases of bulking ocurring in extended aeration plants (the most common type in France). A test method was developed to provide the elements for dimensioning the system. Unreserved cooperation between the owner of the plant, the manager, the SATESE (Service of technical aid to wastewater treatment plant managers) and specialized laboratories is required to implement such measures. Some cases, about which CEMAGREF was consulted, were examined; three of them are detailed and reasons for success or fai1ure are analysed. Seeking funds for the works too often leads to excessive delays.

Phylogenetic analysis and in situ identification of “Nostocoida limicola”-like filamentous bacteria in activated sludge from industrial wastewater treatment plants

2002 ◽  
Vol 46 (1-2) ◽  
pp. 99-104 ◽  
Author(s):  
J. Snaidr ◽  
C. Beimfohr ◽  
C. Levantesi ◽  
S. Rossetti ◽  
J. van der Waarde ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
Molecular Probes ◽  
Special Focus ◽  
Filamentous Bacteria ◽  
Industrial Wastewater Treatment ◽  
Biological Methods

The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened activated sludge samples from different industries with sometimes severe bulking sludge problems. A special focus was paid to filaments which resembled “Nostocoida limicola”, a filamentous bacterium which was found to be present in many WWTPs. These filamentous bacteria are hardly cultivable and only one strain was obtained and maintained in co-culture with a yeast. The 16S rRNA sequences of several other “Nostocoida limicola”-like filamentous bacteria from different sludge samples were obtained by micromanipulation and different molecular-biological methods. The sequences were phylogenetically analyzed and specific molecular probes were developed and applied. The results clearly demonstrate that “Nostocoida limicola”-like filaments from industrial WWTPs are different from all other “Nostocoida limicola” types investigated so far. Our strains are affiliated to the alpha-subclass of Proteobacteria.

scholarly journals Analysis of Bacterial Community Structure of Activated Sludge from Wastewater Treatment Plants in Winter

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Shuang Xu ◽  
Junqin Yao ◽  
Meihaguli Ainiwaer ◽  
Ying Hong ◽  
Yanjiang Zhang
Keyword(s):  
Wastewater Treatment ◽  
Community Structure ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Bacterial Community Structure ◽  
Redundancy Analysis ◽  
Wastewater Treatment Plants ◽  
Effluent Treatment ◽  
Autonomous Region ◽  
Sludge Bulking

Activated sludge bulking is easily caused in winter, resulting in adverse effects on effluent treatment and management of wastewater treatment plants. In this study, activated sludge samples were collected from different wastewater treatment plants in the northern Xinjiang Uygur Autonomous Region of China in winter. The bacterial community compositions and diversities of activated sludge were analyzed to identify the bacteria that cause bulking of activated sludge. The sequencing generated 30087–55170 effective reads representing 36 phyla, 293 families, and 579 genera in all samples. The dominant phyla present in all activated sludge were Proteobacteria (26.7–48.9%), Bacteroidetes (19.3–37.3%), Chloroflexi (2.9–17.1%), and Acidobacteria (1.5–13.8%). Fifty-five genera including unclassified_f_Comamonadaceae, norank_f_Saprospiraceae, Flavobacterium, norank_f_Hydrogenophilaceae, Dokdonella, Terrimonas, norank_f_Anaerolineaceae, Tetrasphaera, Simplicispira, norank_c_Ardenticatenia, and Nitrospira existed in all samples, accounting for 60.6–82.7% of total effective sequences in each sample. The relative abundances of Saprospiraceae, Flavobacterium, and Tetrasphaera with the respective averages of 12.0%, 8.3%, and 5.2% in bulking sludge samples were higher than those in normal samples. Filamentous Saprospiraceae, Flavobacterium, and Tetrasphaera multiplied were the main cause for the sludge bulking. Redundancy analysis (RDA) indicated that influent BOD5, DO, water temperature, and influent ammonia had a distinct effect on bacterial community structures.

Modelling seasonal dynamics of Microthrix parvicella

2006 ◽  
Vol 54 (1) ◽  
pp. 189-198 ◽  
Author(s):  
T. Hug ◽  
W. Gujer ◽  
H. Siegrist
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Future Research ◽  
Model Structure ◽  
Filamentous Bacteria ◽  
Selection Mechanism ◽  
Research Activities ◽  
Microthrix Parvicella ◽  
Selection Mechanisms ◽  
Activated Sludge Systems

The filamentous bacteria “Microthrix parvicella” can cause serious bulking and scumming in wastewater treatment plants (WWTPs) all over the world. Decades of research have identified Microthrixas a specialized lipid consumer but could not clarify the processes that allow this organism to successfully compete in activated sludge systems. In this study we developed a model, based on ASM3, that describes the pronounced seasonal variations of Microthrix abundance observed in a full-scale WWTP. We hypothesize that low temperatures reduce the solubility of lipids and inhibit their uptake by non-specialized bacteria. The presented model structure and parameters successfully fit the measured data; however they do not necessarily reflect the only and true selection mechanism for Microthrix. This model is not yet to be used for prediction; it is rather a valuable research tool to coordinate the discussion and plan future research activities in order to identify the relevant selection mechanisms favoring Microthrix in activated sludge systems.

Identity, abundance and physiology of Aquaspirillum-related filamentous bacteria in activated sludge

2006 ◽  
Vol 54 (1) ◽  
pp. 237-245 ◽  
Author(s):  
T.R. Thomsen ◽  
C. Kragelund ◽  
P.H. Nielsen
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Oligonucleotide Probe ◽  
Filamentous Bacteria ◽  
Type B ◽  
Anaerobic Conditions ◽  
Almost All

Microcolony-forming bacteria closely related to the genus Aquaspirillum in the Betaproteobacteria were recently observed to be abundant in many nutrient removal wastewater treatment plants. The developed oligonucleotide probe, Aqs997, however, occasionally also targeted some filamentous bacteria in activated sludge samples when fluorescence in situ hybridization was performed. In this study, the identity, abundance, and ecophysiology of these Aqs997-positive filamentous bacteria were studied in detail. Most of the Aqs997-positive filamentous bacteria could morphologically be identified as either Eikelboom Type 1701, Type 0041/0675 or possibly Type 1851, all characterized by epiphytic growth. They were found in almost all 21 wastewater treatment plants investigated. Two morphotypes were found. Type A filaments, which seemed to be the same genotype as the microcolony-forming bacteria targeted by probe Aqs997.Type B filaments also hybridized with probe GNS941, specific for the Chloroflexi phylum, so the true identity remains unclear. Aqs997-positive filaments usually stained Gram-negative, but Gram-positive filaments were also found, stressing the difficulties in identifying bacteria from morphology and simple staining results. Studies on the ecophysiology by microautoradiography showed that Aqs997-positive filamentous bacteria did not consume acetate and glucose, while some took up butyrate, mannose, and certain amino acids. Most likely, some Aqs997-positive filamentous bacteria were able to perform full denitrification such as the Aqs997-positive microcolony-forming bacteria, and some were able to store polyhydroxyalkanoates under anaerobic conditions, potentially being glycogen accumulating organisms.

Influence of temperature and sludge loading on activated sludge settling, especially on Microthrix parvicella

1998 ◽  
Vol 37 (4-5) ◽  
pp. 27-35 ◽  
Author(s):  
S. Knoop ◽  
S. Kunst
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Loading Rate ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Causative Organism ◽  
Foam Formation ◽  
Microthrix Parvicella

During recent years modern full scale wastewater treatment plants with biological nitrification, denitrification and phosphorus removal have had increasing problems with foam formation on the surfaces of aerobic tanks and with bulking activated sludge. The results of a survey in 1995 (Kunst and Knoop, 1996) showed that most often the filamentous bacterium Microthrix parvicella is responsible for these problems. Up to today there is only little knowledge about its selection criteria in activated sludge. Therefore several experiments were done in full scale activated sludge plants and in laboratory systems under defined conditions to investigate the influence of low (< 0.1 kg/(kg·d)) and high (≤ 0.2 kg/(kg·d)) BOD5-sludge loading rates on the growth and morphology of M. parvicella and the settlement of activated sludge. Furthermore the influence of temperatures of 5°C, 12°C and 20°C on the growth of M. parvicella was investigated. It was shown that M. parvicella grows at low BOD5-sludge loading rate and low temperature and is the main causative organism of bulking and foaming sludge in nutrient removal plants. On the basis of this investigation it was concluded that the growth of M. parvicella and the settling problems of the activated sludge resulting from excessive growth of this filament will always appear in modern municipal wastewater treatment plants with BOD5-sludge loading rate ≤ 0.1 kg/(kg·d) especially under low temperature conditions.

Foaming in anaerobic digesters caused by Microthrix parvicella

1998 ◽  
Vol 37 (4-5) ◽  
pp. 51-55 ◽  
Author(s):  
Åsa Dillner Westlund ◽  
Eva Hagland ◽  
Maria Rothman
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Anaerobic Digester ◽  
Foam Formation ◽  
Anaerobic Conditions ◽  
Anaerobic Digesters ◽  
Operating Strategy ◽  
Microscopic Studies ◽  
Microthrix Parvicella

Three large wastewater treatment plants in the greater Stockholm area have experienced serious anaerobic digester foaming. Microscopic studies of the sludge from the foam phase showed a network of the filamentous organism Microthrix parvicella. The morphology of the long, coiled filament appeared to be affected by the anaerobic conditions where it became broken up into to shorter and thicker filaments. The operating strategy to prevent foam in the anaerobic digesters at these plants is to control the growth of M. parvicella in the activated sludge tanks by increasing the sludge load. Top installed stirrers and the addition of poly-aluminium salt have also been used to prevent foam formation.

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