A novel optical panel photobioreactor for cultivation of microalgae

2013 ◽  
Vol 67 (11) ◽  
pp. 2543-2548 ◽  
Author(s):  
Hee-Jeong Choi ◽  
Jae-Min Lee ◽  
Seung-Mok Lee
Keyword(s):  
Flat Plate ◽  
Light Source ◽  
Cell Concentration ◽  
Fluorescent Light ◽  
Initial Cell Concentration ◽  
Light Emitting ◽  
Initial Cell ◽  
Fluorescent Lamps ◽  
Parametric Studies ◽  
Optical Panel Photobioreactor

In this study, a novel optical panel photobioreactor (OPPBR) equipped with a V-cut/or flat optical panel (OP) and a light source, i.e., light-emitting diodes (LEDs) was developed. The performance of this OPPBR was assessed using cultures of Chlorella vulgaris. Growth rates of biomass were compared in bioreactors operated separately using a V-cut OP, or a flat-plate OP both equipped with LEDs or a fluorescent light source without any OP. The experiments were conducted at neutral pH (7.2 ± 0.3) with an initial cell concentration of 0.15 ± 0.05 g L−1, at 23 ± 1 °C under dark and light cycles of 8 and 16 h, respectively, using LEDs and fluorescent lamps for 11 days. The results demonstrated that the amount of biomass produced using the V-cut OP was three times higher than the flat-plate OP and five times higher than without the OP. Parametric studies demonstrated that a distance of 3 mm between the OP and the LEDs produced the highest illumination uniformity, i.e., 65.7% for the flat-plate OP and 87.6% for the V-cut OP. The OPPBR system can be scaled up and could be used to enhance biomass production using an LED and OP combination.

Performance of Arabidopsis thaliana under different light qualities: comparison of light-emitting diodes to fluorescent lamp

2017 ◽  
Vol 44 (7) ◽  
pp. 727 ◽  
Author(s):  
Karin Köhl ◽  
Takayuki Tohge ◽  
Mark Aurel Schöttler
Keyword(s):  
Arabidopsis Thaliana ◽  
Light Source ◽  
Light Emitting Diodes ◽  
Seed Mass ◽  
Fluorescent Light ◽  
Chlorophyll Fluorescence Parameters ◽  
Light Emitting ◽  
Led Light ◽  
Improve Energy Efficiency ◽  
Fluorescent Lamps

For precise phenotyping, Arabidopsis thaliana (L.) Heynh. is grown under controlled conditions with fluorescent lamps as the predominant light source. Replacement by systems based on light emitting diodes (LED) could improve energy efficiency and stability of light quality and intensity. To determine whether this affects the reproducibility of results obtained under fluorescent lamps, four Arabidopsis accessions and a phytochrome mutant were grown and phenotyped under two different LED types or under fluorescent lamps. All genotypes had significantly higher rosette weight and seed mass and developed faster under LED light than under fluorescent lamps. However, differences between genotypes were reproducible independent of the light source. Chlorophyll content, photosynthetic complex accumulation and light response curves of chlorophyll fluorescence parameters were indistinguishable under LED and fluorescent light. Principal component analysis of leaf metabolite concentrations revealed that the effect of a change from fluorescent light to LED light was small compared with the diurnal effect, which explains 74% of the variance and the age effect during vegetative growth (12%). Altogether, the replacement of fluorescent lamps by LED allowed Arabidopsis cultivation and reproduction of results obtained under fluorescent light.

The comparison of trichloroethylene removal rates by methane- and aromatic-utilizing microorganisms

1998 ◽  
Vol 38 (7) ◽  
pp. 19-24 ◽  
Author(s):  
C.-J. Lu ◽  
C. M. Lee ◽  
M.-S. Chung
Keyword(s):  
Cell Concentration ◽  
Removal Rate ◽  
Carbon Sources ◽  
Batch Reactors ◽  
Initial Cell Concentration ◽  
Removal Rates ◽  
Initial Cell ◽  
Toluene Concentration ◽  
Cell Source ◽  
Removal Efficiencies

The comparison of TCE cometabolic removal by methane, toluene, and phenol utilizers was conducted with a series of batch reactors. Methane, toluene, or phenol enriched microorganisms were used as cell source. The initial cell concentration was about 107 cfu/mL. Methane, toluene, and phenol could be readily biodegraded resulting in the cometabolic removal of TCE. Among the three primary carbon sources studied, the presence of phenol provided the best cometabolic removal of TCE. When the concentration of carbon source was 3 mg-C/L, the initial TCE removal rates initiated by methane, toluene, and phenol utilizers were 1.5, 30, and 100 μg/L-hr, respectively. During the incubation period of 80 hours, TCE removal efficiencies were 26% and 96% with the presence of methane and toluene, respectively. However, it was 100% within 20 hours with the presence of phenol. For phenol utilizers, the initial TCE removal rates were about the same, when the phenol concentrations were 1.35, 2.7, and 4.5 mg/L. However, TCE removal was not proportional to the concentrations of phenol. TCE removal was hindered when the phenol concentration was higher than 4.5 mg/L because of the rapid depletion of dissolved oxygen. The presence of toluene also initiated cometabolic removal of TCE. The presence of toluene at 3 and 5 mg/L resulted in similar TCE removal. The initial TCE removal rate was about 95 μg/L-hr at toluene concentrations of 3 and 5 mg/L compared to 20 μg/L-hr at toluene concentration of 1 mg/L.

Quality and Intensity of Light in the In Vitro Development of Microstumps of Eucalyptus urophylla in a Photoautotrophic System

2020 ◽  
Vol 66 (6) ◽  
pp. 754-760 ◽  
Author(s):  
Natane A Miranda ◽  
Aloisio Xavier ◽  
Wagner C Otoni ◽  
Ricardo Gallo ◽  
Kellen C Gatti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Light Quality ◽  
Stomatal Density ◽  
Carotenoid Content ◽  
Fluorescent Light ◽  
Eucalyptus Urophylla ◽  
Light Emitting ◽  
Fluorescent Lamps ◽  
Number Of Shoots

Abstract The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.

scholarly journals THE EFFECT OF THE INITIAL CELL CONCENTRATION UPON SURVIVAL OF BACTERIA AT −22 C

1955 ◽  
Vol 69 (3) ◽  
pp. 244-249 ◽  
Author(s):  
Cecil P. Major ◽  
John D. McDougal ◽  
Arthur P. Harrison
Keyword(s):  
Cell Concentration ◽  
Initial Cell Concentration ◽  
Initial Cell

The Effect of Initial Cell Concentration on Xylose Fermentation by Pichia stipitis

2007 ◽  
pp. 653-662 ◽  
Author(s):  
Frank K. Agbogbo ◽  
Guillermo Coward-Kelly ◽  
Mads Torry-Smith ◽  
Kevin Wenger ◽  
Thomas W. Jeffries
Keyword(s):  
Xylose Fermentation ◽  
Cell Concentration ◽  
Pichia Stipitis ◽  
Initial Cell Concentration ◽  
Initial Cell

scholarly journals The Growth ofMonoraphidiumsp. andScenedesmussp. Cells in the Presence of Thorium

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Juliana Cristina de Queiroz ◽  
Ana Cristina de Melo Ferreira ◽  
Antonio Carlos Augusto da Costa
Keyword(s):  
Cell Concentration ◽  
Direct Count ◽  
Initial Cell Concentration ◽  
Initial Cell ◽  
Count Method ◽  
Low Concentrations ◽  
Direct Counting ◽  
Presence And Absence ◽  
Positive Effect

Toxicity of thorium byMonoraphidiumsp. andScenedesmussp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days,Monoraphidiumcells decreased their concentration from4.23×106to4.27×105and8.57×105 cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively.Scenedesmussp. cells were more resistant to thorium: for an initial cell concentration of7.65×104 cells/mL it was observed a change to5.25×105and5.12×105 cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and thatScenedesmuscells are more resistant to thorium thanMonoraphidiumcells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies.

The effect of initial cell concentration on xylose fermentation by Pichia stipitis

2007 ◽  
Vol 137-140 (1-12) ◽  
pp. 653-662 ◽  
Author(s):  
Frank K. Agbogbo ◽  
Guillermo Coward-Kelly ◽  
Mads Torry-Smith ◽  
Kevin Wenger ◽  
Thomas W. Jeffries
Keyword(s):  
Xylose Fermentation ◽  
Cell Concentration ◽  
Pichia Stipitis ◽  
Initial Cell Concentration ◽  
Initial Cell
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