Organic matter stabilization in reed bed systems: Danish and Italian examples

2013 ◽  
Vol 68 (8) ◽  
pp. 1888-1894 ◽  
Author(s):  
Eleonora Peruzzi ◽  
Steen Nielsen ◽  
Cristina Macci ◽  
Serena Doni ◽  
Renato Iannelli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Alkyl Benzene ◽  
Sludge Stabilization ◽  
Organic Matter Stabilization ◽  
Study Results ◽  
Polycyclic Aromatic ◽  
Reed Bed ◽  
Landscape Ecosystems ◽  
Ethylhexyl Phthalate

In this study, results about sludge stabilization in reed bed systems (RBSs) after the entire period of operation in two different systems situated in Denmark (Helsinge 42,000 population equivalent (p.e.) – 10 years) and in Italy (La Fontina, 30,000 p.e. – 6 years) were presented. In order to evaluate the process of sludge stabilization, parameters that highlighted the biochemical and chemico-structural properties of organic sludge matter have been determined. The level of total and soluble nutrients, and enzyme activities, parameters related to overall microbial activity, showed that stabilization of the sludge similarly occurred in both RBSs, even though in different landscape ecosystems. The chemical–structural characterization of sludge organic matter highlighted how the processes of stabilization have occurred satisfactorily in both RBSs; in fact, significant levels of pyrolytic indices for mineralization and humification were reached. The successful stabilization of organic matter occurred in both RBSs and was confirmed by the absence of Escherichia coli, and also by the results of organic compounds (polycyclic aromatic hydrocarbons, linear alkyl benzene sulfonates, nonylphenol ethoxylates, di-2-ethylhexyl-phthalate) and heavy metals.

scholarly journals Sewage sludge and waterworks sludge stabilization in sludge treatment reed bed systems

2017 ◽  
Vol 76 (2) ◽  
pp. 355-363 ◽  
Author(s):  
Grazia Masciandaro ◽  
Eleonora Peruzzi ◽  
Steen Nielsen
Keyword(s):  
Organic Matter ◽  
Point Of View ◽  
Pyrolysis Gas ◽  
Organic Matter Quality ◽  
Sludge Treatment ◽  
Chromatography Analysis ◽  
Sludge Stabilization ◽  
Study Results ◽  
Reed Bed ◽  
Waterworks Sludge

In this study, results about sludge stabilization in sludge treatment reed bed (STRB) systems in two different systems, Hanningfield STRB 1 (England), treating waterworks sludge, and Stenlille STRB 2 (Denmark), treating surplus activated sludge, are presented. The study mainly focused on the effectiveness of the STRBs systems in stabilizing sludge organic matter; in fact, parameters correlated to biochemical and chemico-structural properties of organic sludge matter were determined. Dewatering and sludge stabilization were effective in both STRBs, as highlighted by total and volatile dry solids trend. β-glucosidase, phosphatase, arylsulphatase, leucine amino-peptidase and butyrate esterase activities, enzymes related to C, P, S, N and overall microbial activity, respectively, significantly declined along the profile in both STRBs. The determination of humic carbon highlighted the formation of a stable nucleus of humified organic matter in both STRBs in the deepest layers, thus meaning the successful stabilization of sludge organic matter for both kind of sludges. Similar conclusions can be drawn from pyrolysis gas chromatography analysis (Py-GC), which enables the characterization of soil organic matter quality from a chemical-structural point of view. The pyrolytic indices of mineralization and humification showed that in both STRBs the sludge organic matter is well stabilized.

Organic matter and pollutants monitoring in reed bed systems for sludge stabilization: a case study

2014 ◽  
Vol 22 (4) ◽  
pp. 2447-2454 ◽  
Author(s):  
Eleonora Peruzzi ◽  
Cristina Macci ◽  
Serena Doni ◽  
Maurizio Volpi ◽  
Grazia Masciandaro
Keyword(s):  
Organic Matter ◽  
Sludge Stabilization ◽  
Reed Bed

Pollutant monitoring in sludge treatment wetlands

2011 ◽  
Vol 64 (7) ◽  
pp. 1558-1565 ◽  
Author(s):  
E. Peruzzi ◽  
G. Masciandaro ◽  
C. Macci ◽  
S. Doni ◽  
B. Ceccanti
Keyword(s):  
Heavy Metals ◽  
Organic Matter ◽  
Organic Compounds ◽  
Wastewater Treatment Plants ◽  
Land Application ◽  
Water Soluble ◽  
Sludge Stabilization ◽  
Organic Matter Stabilization ◽  
Urban Wastewater Treatment ◽  
Toxic Organic Compounds

Phragmites australis for sludge dewatering and stabilization processes have been widely proved. The presence of reeds, indeed, efficiently allows solids dewatering and organic matter stabilization in order to obtain a stabilised product that can be suitable for land application, even if its environmental impact has to be considered. The actual revision of the European Union's Working Document on Sludge (2000), in fact, seems to be addressed to detect two principal categories of pollutants in sludge for agricultural use: heavy metals and toxic organic compounds. In this study are presented results about sludge stabilization and monitoring of heavy metal fractionation and organic compounds in four urban wastewater treatment plants managed by Acque S.p.A., (Tuscany, Italy). To evaluate the process of sludge stabilization parameters were determined that highlight the biochemical and chemico-structural properties of sludge organic matter. The results showed that stabilization of the sludge over time occurred as shown by the low content of water soluble carbon and dehydrogenase activity, and by the re-synthesis of humic-like matter highlighted by the pyrolytic indices of mineralization and humification. Results about fractionation showed that heavy metals were retained in fractions related to the stabilized organic matter. Moreover, toxic organic compounds showed a drastic reduction at the end of the monitoring period.

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

Immunochemical Characterization of Setaria cervi Microfilarial Antigens Using Novel Antibodies

2019 ◽  
Vol 19 (14) ◽  
pp. 1263-1274 ◽  
Author(s):  
Anuradha Kalani ◽  
Komal Kalani ◽  
Poonam Chaturvedi ◽  
Pankaj Chaturvedi
Keyword(s):  
Adult Worm ◽  
Diagnostic Tests ◽  
Protective Immunity ◽  
Complex Nature ◽  
Immune Recognition ◽  
Setaria Cervi ◽  
Crossed Immunoelectrophoresis ◽  
Study Results ◽  
Immune Rabbit

Background:Filariasis affects millions of people in tropical and subtropical regions of the world and is caused by nematode roundworm. In order to develop a vaccine and specific diagnostic tests, it is important to characterize different stages of the filarial worms. Microfilariae (Mf) stage of the roundworm is found in host’s blood or lymph vessels and can be important not only for developing better immunodiagnostics but also for understanding immune recognition and its relevance to immunepathogenesis and protective immunity.Objective:The present study aimed to immunocharacterize Mf and adult worm antigens that could be helpful in future diagnostic tests.Method:Four different immune sera against Setaria cervi intact live, intact live with adjuvant, intact glutaraldehyde fixed with adjuvant and total somatic Mf were prepared and used for the immunocharacterization of Mf antigens.Results:Our study results suggest that compared to fixed intact Mf, live intact Mf are more immunogenic, as the immune sera generated against intact live Mf showed high ELISA reactivity with Setaria cervi Mf and adult worm antigens. All the four immune sera IgG fractions had surface specificity as determined through considerable ELISA reactivity with S. cervi intact Mf. When tested under native conditions (immunoelectrophoresis and crossed immunoelectrophoresis), all the four immune rabbit sera were able to detect antigens of S. cervi Mf and adult stages.Conclusion:These results can be useful in detailed understanding of the complex nature of the Mf and adult antigens, which are prerequisites in the development of vaccine and more specific diagnostic tests.

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